Spleen - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Mouse Mouse, 4 week Trophoblast Collagenase Type 1: 0.1%
Collagenase Type 2: 0.25%
 DMEM Hou, M., Han, J., Li, G., Kwon, M., Jiang, J., Emani, S., Taglauer, E., Park, J., Choi, E., Vodnala, M., Fong, Y., Emani, S., Rosas, I., Perrella, M. and Liu, X.: Multipotency of Mouse Trophoblast Stem Cells, Stem Cell Res Ther 11, 55, 2020
Mouse Mouse Speen Collagenase Type 5: 5%
Hyaluronidase: 2.5%
Elastase: 1.25%
 HBSS Villarreal, E., Hewgley, W., Lang, W., Morton, C., Mao, S., Wu, J. and Sandoval, J.: In-bag Enzymatic Splenic Digestion: A Novel Alternative to Manual Morcellation?, J Surg Res 218, 209-216, 2017
Mouse Mouse Splenic Collagenase Type 2: 0.1%
 PBS Klebanoff, C., Spencer, S., Torabi-Parizi, P., Grainger, J., Roychoudhuri, R., Ji, Y., Sukumar, M., Muranski, P., Scott, C., Hall, J., Ferreyra, G., Leonardi, A., Borman, Z. et al.: Retinoic Acid Controls the Homeostasis of Pre-cDC-Derived Splenic and Intestinal Dendritic Cells., J Exp Med 210, 1961, 2013
Mouse Mouse Splenocytes Collagenase Type 2: 0.16%
Deoxyribonuclease I: 0.002%
 PBS Yi, T. and Cyster, J.: EBI2-Mediated Bridging Channel Positioning Supports Splenic Dendritic Cell Homeostasis and Particulate Antigen Capture., Elife 2, e00757, 2013
Mouse Mouse Spleen, bone marrow endothelial Collagenase Type 4: 0.3-1.0%
Deoxyribonuclease I: 20 u/ml
 PBS Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands., Immunity 34, 590, 2011
Mouse Mouse, female, 6-8 week Dendritic Collagenase Type 4: 0.05%
 RPMI 1640 Abou Fakher, F., Rachinel, N., Klimczak, M., Louis, J. and Doyen, N.: TLR9-Dependent Activation of Dendritic Cells by DNA from Leishmania Major Favors Th1 Cell Development and the Resolution of Lesions., J Immunol 182, 1386, 2009
Mouse Mouse Dendritic Collagenase Type 1: 0.5%
 HBSS Flano, E., Jewell, N., Durbin, R. and Durbin, J.: Methods Used to Study Respiratory Virus Infection.,Curr Protoc Cell Biol Vol. Chapter 26,, , Unit 26.3, 2009
Mouse Mouse Dentritic Collagenase: 300 u/ml
Deoxyribonuclease I: 0.002%
 RPMI 1640 Abe, K., Nguyen, K., Fine, S., Mo, J., Shen, C., Shenouda, S., Corr, M., Jung, S., Lee, J., Eckmann, L. and Raz, E.: Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells., Proc Natl Acad Sci U S A 104, 17022, 2007
Mouse Mouse Splenic Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.1%
 RPMI Mueller, S., Matloubian, M., Clemens, D, Sharpe, A., Freeman, G., Gangappa, S., Larsen, C. and Ahmed, R.: Viral Targeting of Fibroblastic Reticular Cells Contributes to Immunosuppression and Persistence During Chronic Infection., Proc Natl Acad Sci U S A 104, 15430, 2007
Mouse Mouse Splenic dendritic Collagenase: 0.1%
Deoxyribonuclease I: 0.005%
 DMEM Kang, H., Liu, M. and Datta, S.: Low-dose Peptide Tolerance Therapy of Lupus Generates Plasmacytoid Dendritic Cells That Cause Expansion of Autoantigen-Specific Regulatory T Cells and Contraction of Inflammatory Th17 Cells, J Immunol 178, 7849-58, 2007
Mouse Mouse Splenic stromal Collagenase Type 3: 100-400 u/ml
 HBSS Benedict Chris A, De Trez Carl, Schneider Kirsten, Ha Sukwon, Patterson Ginelle, Ware Carl F: Specific remodeling of splenic architecture by cytomegalovirus, PLoS Pathog 2, e16, 2006
Mouse Mouse Leukocytes Collagenase Type 4: 43 u/ml
 RPMI 1640 Siragam, V., Crow, A., Brinc, D., Song, S., Freedman, J. and Lazarus, A.: Intravenous Immunoglobulin Ameliorates ITP via Activating Fc Gamma Receptors on Dendritic Cells., Nat Med 12, 688, 2006
Mouse Mouse, 4-6 week Dentritic Collagenase Type 1: 0.1%
Deoxyribonuclease I: 0.001%
 RPMI-1640 McLellan, A., Kapp, M., Eggert, A., Linden, C., Bommhardt, U., Brocker, E., Kammerer, U. and Kampgen, E.: Anatomic Location and T-cell Stimulatory Functions of Mouse Dendritic Cell Subsets Defined by CD4 and CD8 Expression, Blood 99, 2084-93, 2002
Mouse Mouse Dentritic Collagenase Type 3: 0.1%
Deoxyribonuclease I: 325 u/ml
 RPMI 1640 Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells, J Exp Med 196, 1415, 2002
Mouse Mouse Dentritic cells Collagenase Type 4: 0.1%
Deoxyribonuclease I: 20 ug/ml
 HEPES/RPMI 1640 Stagg, AJ, Burke, F, Hill, S and Knight, S.: Isolation of Mouse Spleen Dendritic Cells., Methods Mol Med 64, 9-22, 2001
Mouse Mouse Dentritic Collagenase: 100 u/ml
 HBSS Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures, Blood 96, 3029-39, 2000