Worthington Tissue Dissociation Guide

Dissociating Enzymes: Chymotrypsin

Chymotrypsin is a protease which preferentially catalyzes the hydrolysis of peptide bonds involving the aromatic amino acids tyrosine, phenylalanine, and tryptophan. In addition it acts upon the peptide bonds of leucyl, methionyl, asparagenyl and glutamyl residues, and the amides and esters of susceptible amino acids.

Chymotrypsin is used to a limited extent in tissue dissociation, usually in combination with trypsin and elastase.

More Information: Worthington Chymotrypsin

Next: Deoxyribonuclease I

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat Adrenal Bone Brain
Cartilage Colon Endothelial Epithelial
Eye Heart Intestine Kidney
Liver Lung Lymph nodes Mammary
Miscellaneous Muscle Neural Pancreas
Parotid Pituitary Prostate Reproductive
Scales Skin Spleen Stem
Thymus Thyroid/Parathyroid Tonsil Tumor

Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.