Worthington Announcements

(last updated 05/14/12)

AAI Survey Winner!

Kristine-Ann Buela of the Eye and Ear Institute of Pittsburgh, PA is the winner of the iPod for participating at the 2012 Immunology (AAI) 2012 annual meeting cell isolation survey held in Boston. Congratulations to Kristine and thanks to all survey participants!

Be sure to watch for additional Worthington surveys and contests at upcoming meetings.

EB 2012 Survey Winner!

Veronica Azcutia of Brigham Women's Hospital/Harvard is the winner of the iPod for participating at the 2012 Experimental Biology (EB) 2012 annual meeting cell isolation survey held in San Diego. Congratulations to Veronica and thanks to all survey participants!

Be sure to watch for additional Worthington surveys and contests at upcoming meetings.

Hepatocyte Isolation System Available!

Optimized and Functionally Tested Enzyme System For Hepatocyte Isolation 
The Worthington Hepatocyte Isolation System has been developed to provide researchers with a reliable, convenient, and consistent hepatocyte cell isolation system.

By using the pre-optimized combination of enzymes contained in this kit, it is possible to minimize the lot-to-lot variation and improve the quality of the isolated hepatocytes.In addition, Worthington use-tests each lot by isolating hepatocytes from adult rat to assure performance, reliability, and consistent yield of viable cells.

Each kit includes single-use enzyme vials, balanced salt solution, buffer, culture media and an optimized protocol for five separate adult rat liver perfusions. The method is based on that described by Berry, M.N., modified by Seglen, P.O., and further optimized in conjunction with several collaborating researchers.

Available on-line at www.worthington-biochem.com, e-mail: techservice@worthington-biochem.com , Call: 800.445.9603/732.942.1660 or Fax: 732-942-9270 for additional information!

ISO9001:2008 Certification

Worthington Biochemical Corporation is proud to announce their successful company-wide ISO9001:2008 upgrade recertification upon completion of the three year reassessment audit by ANAB-accredited SGS US Testing Company, Systems & Services Certification.

Worthington Biochemical develops and produces high quality purified enzymes, proteins, nucleic acids and cell isolation kits for applications in life science research, diagnostics, bioprocessing, biopharm & biotechnology. Founded in 1947 and privately held, Worthington has maintained a reputation for quality products and excellent customer service which their ISO9001:2008 recertification confirms.

With worldwide distribution, Worthington markets products direct to domestic customers and internationally through a network of distributors. Products are supplied for primary cell isolation & cell culture, protein research, molecular biology and many other research applications. As a primary manufacturer, Worthington can meet enzyme requirements from research-scale to bulk bioprocessing quantities with all manufacturing taking place at their facility located in Lakewood, NJ. 

For additional information: Ph: 800.445.9603 • 732.942.0660, Fax: 800.368.3108, 732.942.9270 or visit www.worthington-biochem.com.

Contact: Jim Zacka, VP, ph: 732.942.1660, e-mail: jzacka@worthington-biochem.com

ASCB 2011 iPod Winner

Kari Eckland of Colorado State University is the winner of the iPod for participating at the 2011 ASCB annual meeting cell isolation survey drawing held in Denver. Congratulations to Kari and thanks to all participants!

Be sure to watch for additional Worthington surveys and contests at upcoming meetings.

ISSCR 2011 STEM & PRIMARY CELL ISOLATION WINNER!

Brenda Takabe from the University of Toronto is the winner of the iPod for participating in the 2011 International Society for Stem Cell Research (ISSCR) annual meeting Stem & Primary Cell Isolation Survey drawing. Congratulations to Brenda and thanks to all participants!

Be sure to watch for additional surveys and contests at upcoming meetings.

CYTO2011 iPod Winner

Bunny Anne Cotleua of The Cleveland Clinic is the winner of the iPod for participating in the 2011 Cytometry (ISAC) annual meeting Primary Cell Isolation Survey drawing. Congratulations to Bunny and thanks to all participants!

Be sure to watch for additional surveys and contests at upcoming meetings.

Experimental Biology 2011 iPod Winner!

Praveen Shukla of North Dakota State University is the winner of the iPod for participating in the 2011 Experimental Biology annual meeting Primary Cell Isolation Survey drawing. Congratulations to Praveen and thanks to all participants!

Be sure to watch for additional surveys and contests at upcoming meetings.

ASCB iPod Winner!

Paul Fletcher of East Carolina University is the winner of the iPod for participating at the 2010 ASCB annual meeting Primary Cell Isolation Survey drawing. Congratulations to Paul and thanks to all participants!

Be sure to watch for additional surveys and contests at upcoming meetings.

Neuroscience Survey Winner!

Rajashekar Iyer of the University of Illinois, Urbana is the winner of the iPod for participating at the 2010 Neuroscience annual meeting Neural Cell Isolation Survey drawing. Congratulations to Raj and thanks to all participants!

Be sure to watch for additional surveys and contests at upcoming meetings.

ISSCR Survey Winner!

Suzanne Pontow of the University of California, Davis is the winner of the iPod for participating at the 2010 ISSCR annual meeting Stem Cell Isolation Survey drawing. Congratulations to Suzanne and thanks to all participants!

Be sure to watch for additional surveys and contests at upcoming meetings.

Experimental Biology Survey Winner!

Rachael Farah-Abraham of the Georgia State University is the winner of the iPod for participating at the 2010 Experimental Biology annual meeting Cell Isolation Survey drawing held in Anaheim, CA. Congratulations to Rachael and thanks to all participants!

Be sure to watch for additional surveys and contests at upcoming meetings.

New Sales Rep for Texas

Worthington Biochemical is pleased to announce the appointment of Paulette Kovar as Sales Representative for Texas. Worthington is well-known as a primary producer of high-quality enzymes, related biochemicals and kits for cell culture, molecular biology and other life-science research applications. Paulette has extensive sales experience and will be visiting soon to meet and learn more about researcher's enzyme needs within Texas. We have recognized and combined these strengths in order to provide Worthington products to Texas researchers with even better service. Please join us in welcoming Paulette to the Worthington team!

Paulette can be reached at cell#: 281.455.4507 or Email: paulette.kovar@earthlink.net

New Sales Rep for the Midwest

Worthington Biochemical is pleased to announce the appointment of Angela Slepicka as Sales Representative for Iowa, Illinois, Missouri, Wisconsin and Kansas City, KS. Worthington is well-known as a primary producer of high-quality enzymes, related biochemicals and kits for cell culture, molecular biology and other life-science research applications. Angela has extensive sales experience and will be visiting soon to meet and learn more about  researcher's enzyme needs within her territory. We have recognized and combined these strengths in order to provide Worthington products to local researchers with even better service. Please join us in welcoming Angela to the Worthington team!

Angela can be reached at Cell#: 630.378.4757 or Email: angsam428@yahoo.com

Congratulations to the ASCB Survey iPod Winner!

Karen Vikstrom of the University of Michigan is the winner of the iPod for participating at the 2009 ASCB annual meeting cell isolation survey drawing held in San Diego. Congratulations to Karen and thanks to all participants!

Be sure to watch for additional surveys and contests at upcoming meetings.

Worthington #19 on NJBIZ New Jersey's Top Biotechnology Firms

Worthington Biochemical Corporation, Lakewood, NJ, has been listed as New Jersey's 19th largest biotechnology company in NJBIZ's October 5, 2009 issue.

Founded in 1947 by Charles Worthington and privately held, the company gained one spot in the ranking from the 2010 NJBIZ list, currently employing 55 in the Garden State.

Worthington is an ISO9001 certified producer of purified enzymes, proteins, nucleic acids and kits for life science research, diagnostic, biopharmaceutical, biotechnology and bioprocessing applications.

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Congratulations To The ENDO '09 iPod Winner!

Erika Ginsburg from the NIH/NCI is the winner of the iPod for participating at the 2009 ENDO annual meeting drawing held in Washington, DC, in June, 2009. Congratulations to Erika and thanks to all participants!

Be sure to watch for additional survey contests at upcoming meetings.

Congratulations To The EB '09 iPod Winner!

Tim Angelotti of Stanford University is the winner of the iPod for participating at the 2009 Experimental Biology annual meeting drawing held in New Orleans, LA, in April, 2009. Congratulations to Tim and thanks to all participants!

Be sure to watch for additional contests at upcoming meetings.

Animal Origin Free Collagenase Available!

Researchers interested in regenerative tissue applications and/or avoiding potential viral and prion risks from commonly used animal sourced fermentation media components now have Animal Origin Free (AOF) Collagenase produced by Worthington.

Type AFA collagenase is derived from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of potential animal derived pathogens must be prevented is now available from Worthington. Levels of secondary proteases are similar to Types 1 and 2 collagenase.

Several lots of AOF Collagenase Type AFA are now available for sampling and evaluation in your specific application(s). Please contact Technical Service at 800.445.9603, e-mail: techservice@worthington-biochem.com or request samples on-line, Collagenase Sampling Program.

For more information.

Animal Origin Free Recombinant (AOF) DNase I Available!

Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast, Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials is now produced by Worthington.

Bovine pancreas is a rich source of RNase A which is often found in many commercial DNase preparations. Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNase greatly facilitates purification of an enzyme with undetectable levels of RNase. The processes involved in the production and isolation of recombinant DNase I are completely devoid of animal based components which eliminates the possibility of introducing animal derived pathogens into bioprocessing procedures.

Recombinant DNase I is suitable for such applications as:
• Removing genomic DNA from RNA preparations prior to RT-PCR
• Degradation of DNA templates after transcription reactions
• Removing unwanted DNA from samples prior to Northern blotting
• Removing DNA during biopharma and bioprocessing procedures

For more information.

Congratulations To The ASCB '08 Survey Winner!

Tamlyn Thomas of the University of Rochester is the winner of the iPod for participating in Worthington's Cell Isolation Survey at the 2008 American Society for Cell Biology (ASCB) annual meeting held in San Francisco, CA December, 2008. The survey information will help us better meet the needs of all primary cell isolation researchers. Congratulations to Tamlyn and thanks to all survey participants!

New Sequencing Proteases Now Available for Proteomics

Worthington now offers specially packaged and characterized forms of Staph Protease (Endo-Glu-C) and Chymotrypsin for sequencing and proteomics applications.

Protease, S. aureus (Endo-Glu-C), Sequencing Grade is chromatographically purified according to Drapeau, G, et.al, JBC, 247, 6720 (1972). Supplied in multiple vials containing 10µg or 50µg lyophilized powder.

Chymotrypsin, Alpha, TLCK Treated, Sequencing Grade is 3X crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed to remove autolysis products and low molecular weight contaminants. Supplied as lyophilized powder in multiple 25ug & 100ug vials.

These and all Worthington products may be ordered by phone (800.445.9603), fax (800.368.3108) or online at www.worthington-biochem.com.

New Sales Rep For Colorado & Utah!

Worthington Biochemical is pleased to announce the appointment of Dave Koehler and Alphatek, Inc. as Sales Representative for Colorado and Utah. Worthington is well-known as a primary producer of high-quality enzymes, related biochemicals and kits for cell culture, molecular biology and other life-science research applications. Dave has extensive sales experience and will visiting soon to meet and learn more about Colorado and Utah researcher's enzyme needs . We have recognized and combined these individual strengths in order to provide Worthington products to Colorado researchers with even better service.

Please join us in welcoming Dave to the Worthington team!

Dave can be reached at Ph: 303.845.1836, Cell: 303.588.8908 or Email: dave@alphatek-inc.com

ISO9001:2000 RECERTIFICATION OF WORTHINGTON BIOCHEMICAL CORPORATION

Worthington Biochemical Corporation is proud to announce their successful company-wide ISO9001:2000 recertification upon completion of the three year reassessment audit by ANAB-accredited SGS US Testing Company, Systems & Services Certification.

Worthington Biochemical develops and produces high quality purified enzymes, proteins, nucleic acids and cell isolation kits for applications in life science research, diagnostics, bioprocessing, biopharm & biotechnology. Founded in 1947 and privately held, Worthington has maintained a reputation for quality products and excellent customer service which their ISO9001:2000 recertification confirms.

With worldwide distribution, Worthington markets products direct to domestic customers and internationally through a network of distributors. Products are supplied for primary cell isolation & cell culture, protein research, molecular biology and many other research applications. As a primary manufacturer, Worthington can meet enzyme requirements from research-scale to bulk bioprocessing quantities with all manufacturing taking place at their facility located in Lakewood, NJ.

For additional information: Ph: 800.445.9603 • 732.942.0660, Fax: 800.368.3108, 732.942.9270 or visit www.worthington-biochem.com.

Contact: Jim Zacka, VP, ph: 732.942.1660, e-mail: jzacka@worthington-biochem.com

Collagenase Product(s) Raw Material Certification

Although it does not affect the majority of research customers, please be aware that the FDA has recently issued a 'Risk of BSE in Collagenase Enzymes' notice to all FDA-regulated users of Collagenases. (5/23/07 FDA Collagenase Note: PDF Version) Worthington currently provides Collagenase to many such customers for which this issue is likely to arise. We recently drafted a document (Collagenase Products Raw Material Certification) in response to the FDA's letter to all regulated Collagenase user, available on our website. Please note that although Worthington is not currently a cGMP manufacturer of collagenase we operate under GMP guidelines and are ISO9001:2000 Certified. In addition and most importantly, Worthington has never used Brain Heart Infusion (BHI) media in our collagenase production process. Other low-risk animal sourced raw materials are of USDA or equivalent inspected and approved as suitable for human consumption. We have also been working on developing a completely Animal Origin Free (AOF) collagenase product and have raised the priority of this due to recent inquiries such as this. We hope to have AOF Collagenase evaluation material available for sampling within a few months. We would welcome the opportunity to provide evaluation samples as soon as they become available if interested. Please let us know if we can be of additional assistance or if any additional information is required by any of your customers. We look forward to continuing to provide collagenase to meet the technical and regulatory requirements of the market.

HEPATOCYTE ISOLATION SYSTEM AVAILABLE!

Optimized and Functionally Tested Enzyme System For Hepatocyte Isolation
The Worthington Hepatocyte Isolation System has been developed to provide researchers with a reliable, convenient, and consistent hepatocyte cell isolation system. By using the pre-optimized combination of enzymes contained in this kit, it is possible to minimize the lot-to-lot variation and improve the quality of the isolated hepatocytes.

In addition, Worthington use-tests each lot by isolating hepatocytes from adult rat to assure performance, reliability, and consistent yield of viable cells. Each kit includes single-use enzyme vials, balanced salt solution, buffer, culture media and an optimized protocol for five separate adult rat liver perfusions. The method is based on that described by Berry, M.N., modified by Seglen, P.O., and further optimized in conjunction with several collaborating researchers.

Available on-line at www.worthington-biochem.com, e-mail: techservice@worthington-biochem.com , Call: 800.445.9603/732.942.1660 or Fax: 732-942-9270 for additional information!

Collagenase Lot Selection Tool Available Online!

Worthington's 'Collagenase Lot Selection Tool' is available online at our website. This new feature was designed to help researchers select and evaluate current collagenase lots that match previous lots or desired activity profiles. Users may enter target values for collagenase, caseinase, clostripan, and tryptic activities or specify previous lot numbers.

Each value can be weighted based on the relative level of importance to the application. After the search for matches is completed, a ranked list of collagenase lots currently available is generated. The selected lots can then be sampled simply by using the built in link to the Free Collagenase Sampling Program.

As always, Worthington Customer and Technical Service personnel are available via phone at 800.445.9603/732.942.1660 and e-mail to assist with collagenase or any other products.

Product Certificates of Analysis Available Online

Certificates of Analysis for any Worthington product are available online at our website, http://www.worthington-biochem.com.

This feature will enable customer's to instantly obtain a Certificate of Analysis with assay and descriptive information for any lot of Worthington product. Simply enter the desired lot number to obtain the current, lot-specific information. As always,

Worthington Customer and Technical Service personnel are available via phone at 800.445.9603/732.942.1660 and e-mail to assist with this or any other issues.

New! On-Line Ordering Now Available

Customers worldwide can now order Worthington products on-line through our new, easy to use on-line ordering system. Simply click the 'On-Line Ordering' link on our homepage and enter the requested information. As always, customer assistance and technical service is available BY PHONE: (732) 942-1660 or 1-800-445-9603, BY FAX: (732) 942-9270 or 1-800-368-3108 and e-mail.

NEW Primary Cell Isolation & Tissue Dissociation Guide Available

Worthington's latest 12th edition Tissue Dissociation Guide is now available to assist researchers with selecting enzymes, developing and optimizing protocols and better understanding the process of enzymatic primary cell isolation and tissue dissociation.

The guide also discusses the enzymes most commonly used, their handling and specificity, and includes hundreds of new detailed cell and species-specific references. Available online at www.tissuedissociation.com
Request a copy online, by phone (800.445.9603 or 732.942.1660), or by Fax (732.942.9270).

New Tissue Dissociation Website

Worthington has launched a new website: www.tissuedissociation.com
It contains all information published in our popular Tissue Dissociation Guide. Also, annotated references ("Tissue Tables") will be updated regularly. You can use the Advanced Search feature to find references relevant to your research.

E•RASE™ RNase A/T1 Blend Digests RNA Faster!

Worthington E•Rase™ Ribonuclease Blend is an optimized blend of purified ribonucleases with differing base cleavage specificities designed to achieve more complete digestion of target RNAs in various applications requiring its removal such as plasmid purification, ribonuclease protection assays, RT-PCR, etc. Bovine pancreatic RNase A cleaves RNA after U and C nucleotides whereas RNase T1 preferentially cleaves after G.

E•RASE™ Ribonuclease Blend contains an optimized combination of these enzymes that digests RNAs more efficiently than either one alone. E•Rase™ contains 0.5mg/ml of RNase A and 100,000u/ml of RNase T1, prepared in a 50% glycerol solution for convenient storage and use, and tested to be free of contaminating DNase and Protease.
For more information.