Tumor - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Hamster Hamster, 90-100 g Tumor Hyaluronidase: 0.1%
 Waymouth's MB Gonzalez, A., Oberley, T., Schultz, J., Ostrom, J., and Li, J.: In Vitro Charactrization of Estrogen Induced Syrian Hamster Renal Tumors: Comparison with an Immortalized Cell Line Derived from Diethylstilbestrol-Treated Adult Hamster Kidney, In Vitro Cell Dev Biol 29A, 562, 1993
Hamster Hamster, 6 week old Buccal pouch Neutral Protease: 0.24%
 CMF HBSS Min, B., Kim, K., Cherrick, H., and Park, N.: Three Cell Lines from Hamster Buccal Pouch Tumors Induced by Topical 7,12-Dimethylbenz(a)Anthracene, Alone or in Conjunction with Herpes Simplex Virus Inoculation, In Vitro Cell Dev Biol 27A, 128, 1991
Human Human Prostate stromal cells Collagenase Type 1: 0.1%
 RPMI 1640 Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi: Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R, Am J Pathol 165, 533-40, 2004
Human Human, adult Human synovial sarcoma Collagenase Type 2: 200 u/ml
 DMEM/F-12 Nishio Jun, Iwasaki Hiroshi, Ishiguro Masko, Ohjimi Yuko, Fujita Chikako, Isayama Teruto, Naito Masatoshi, Oda Yoshinao, Kaneko Yasuhiko, Kikuchi Masahiro: Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor, Int J Oncol 21, 17-23, 2002
Human Human Colonocytes Collagenase:
DMEM/F12 Emenaker N, Calaf G, Cox D, Basson M and Qureshi N: Short chain fatty acids differentially modulate cellular phenotype and c-myc protein levels in primary human nonmalignant and malignant colonocytes, J Nutr 46, 96-105, 2001
Human Human Epithelial, fibroblasts Trypsin: 0.25%
 Ham's F-12 MacLeod, R: Rapid Monolayer Primary Cell Culture from Tissue Biopsy,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons Ltd, 3E:2.1, 1995
Human Human Colon adenocarcinoma Hyaluronidase: 100 u/ml
 DMEM Hague, A and Paraskeva, C: Colon Adenocarcinoma Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12C:1.1, 1995
Human Human Tumor, breast Hyaluronidase: 100 u/ml
 DMEM Beaupain, R., Mainquene, C., Brouty-Boye, D., Planchon, P., and Magniew, V.: "Normal" Breast Cells Adjacent to a Tumor Grown in Long-term Three Dimensional Culture, In Vitro Cell Dev Biol 29, 100, 1993
Human Human Glioma Hyaluronidase: 0.01%
 HBSS Kruse, C., Mitchell, D., Kleinschmidt-DeMasteis, B., Franklin, W., Morse, H., Spector, E., and Lillehei, K.: Characterization of a Continuous Human Glioma Cell Line DBTRG-OSMG: Growth Kinetics, Karyotype, Receptor Expression and Tumor Suppressor Gene Analyses, In Vitro Cell Dev Biol 28, 609, 1992
Human Human Epithelial Collagenase: 2.0%
 DMEM/Ham's F-12 Emerman, J. and Wilkinson, D.: Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples, In Vitro Cell Dev Biol 26, 1186, 1990
Human Human Tumor Neutral Protease: 0.24%
 DMEM/Ham's F-12 Boyd, J., Rinehart Jr., C., Walton, L., Siegal, G. and Kaufman, D.: Ultrastructural Characterization of Two New Human Endometrial Carcinoma Cell Lines and Normal Human Endometrial Epithelial Cells Cultured on Extracellular Matrix, In Vitro Cell Dev Biol 26, 701, 1990
Human Human, 9-74 year Neurofibroma Neutral Protease: 1.25 u/ml
Collagenase Type 1: 0.05%
Hyaluronidase: 0.1%
 L-15 Sheela S, Riccardi VM, Ratner N: Angiogenic and invasive properties of neurofibroma Schwann cells, J Cell Biol 111, 645-53, 1990
Human Human Tumor Trypsin: 0.05%
 DMEM Sacks, P., Parnes, S., Gallick, G., Mansouri, Z., Lichtner, R., Satya-Prakash, K., Pathak, S, and Parsons, D.: Establishment and Characterization of Two New Squamous Cell Carcinoma Cell Lines Derived from Tumors of the Head and Neck, Cancer Res 48, 2858, 1988
Human Human Tumor, colon Trypsin: 0.25%
 McCoy's Brattain, M., Marks, M., McCombs, J., Finely, W., and Brattain, D.: Characterization of Human Colon Carcinoma Cell Lines Isolated From a Single Primary Tumour, Br J Cancer 47, 373, 1983
Human Human Epthelial and tumor
Colon		 Collagenase: 300 u/ml
 PBS medium 199 or medium F 12 Friedman, E., Higgins, P., Lipkin, M., Shinya, H., and Gelb, A.: Tissue Culture of Human Epithelial Cells from Benign Colonic Tumors, In Vitro 17, 632, 1981
Human Human Tumor, breast Neuraminidase: 0.8 u/ml
 HBSS Leung, C., and Shiu, R.: Morphological and Proliferative Characteristics of Human Breast Tumor Cells Cultured on Plastic and in Collagen Matrix, In Vitro 18, 476, 1981
Human Human Melanoma
Metastatic tumors		 Collagenase Type 3: 0.10%
 DMEM Creasey, A., Smith, H., Hackett, A., Fukuyama, K., Epstein, W., and Madin, S.: Biological Properties of Human Melanoma Cells in Culture, In Vitro 15, 342, 1979
Human Human Mammary tumors, hard Collagenase: 0.10%
 RPMI-1640 w/ 5% Fetal Calf Serum Lasfargues, E.: Tissue Culture Methods/Applications,Kruse, P., and Patterson, M., Academic Press, 45, 1973
Mouse Mouse, 30 week Tumor endothelial Collagenase Type 4: 500 u/ml
Collagenase Type 2: 550 u/ml
Deoxyribonuclease I: 3 u/ml
 PBS Berger Mario, Bergers Gabriele, Arnold Bernd, Hämmerling GünterJ, Ganss Ruth: Regulator of G-protein signaling-5 induction in pericytes coincides with active vessel remodeling during neovascularization, Blood 105, 1094-101, 2005
Mouse Mouse, 8-10 week Tumor associated endothelial cells Collagenase Type 2:
see reference Hida Kyoko, Hida Yasuhiro, Amin Dhara N, Flint Alan F, Panigrahy Dipak, Morton Cynthia C, Klagsbrun Michael: Tumor-associated endothelial cells with cytogenetic abnormalities, Cancer Res 64, 8249-55, 2004
Mouse Mouse Pancreatic tumor Collagenase Type 2: 0.5%
Collagenase Type 4: 0.5%
Deoxyribonuclease I: 0.2%
 PBS Bergers Gabriele, Song Steven, Meyer-Morse Nicole, Bergsland Emily, Hanahan Douglas: Benefits of targeting both pericytes and endothelial cells in the tumor vasculature with kinase inhibitors, J Clin Invest 111, 1287-95, 2003
Mouse Mouse Tumor-infiltrating lymphocyte Collagenase: 200 u/ml
 RPMI 1640 Uekusa Yasuhiro, Yu Wen-Gong, Mukai Takao, Gao Ping, Yamaguchi Nobuya, Murai Masako, Matsushima Kouji, Obika Satoshi, Imanishi Takeshi, Higashibata Yuji, Nomura Shintaro, Kitamura Yukihiko, Fujiwara Hiromi, Hamaoka Toshiyuki: A pivotal role for CC chemokine receptor 5 in T-cell migration to tumor sites induced by interleukin 12 treatment in tumor-bearing mice, Cancer Res 62, 3751-8, 2002
Mouse Mouse, male Melanoma tumor cells Collagenase Type 2: 0.5%
 DMEM Arbiser JL, Raab G, Rohan RM, Paul S, Hirschi K, Flynn E, Price ER, Fisher DE, Cohen C, Klagsbrun M: Isolation of mouse stromal cells associated with a human tumor using differential diphtheria toxin sensitivity, Am J Pathol 155, 723-9, 1999
Mouse Mouse (BALB/cfC3H) Mammary tumors
Epithelial		 Collagenase: 1.0%
 HBSS Yang, J., Guzman, R., Richards, J., and Nandi, S.: Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels, In Vitro 16, 502, 1980
Mouse Mouse, BALB/cfC3H/Crgl Neoplastic
Epithelial tumor		 Trypsin:
DMEM Hosick, H.: A Note on Growth Patterns of Epithelial Tumor Cells in Primary Culture, Cancer Res 34, 259, 1974
Mouse Mouse, lactating, 14 day Mammary Trypsin NF 1:250: 0.25%
 HBSS Kopelovich, L., Abraham, S., McGrath, H., DeOme, K., Chaikoff, I.: Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland, Cancer Res 26, 1534, 1966
Rat Rat, male 12 week Sponge infiltrating cells Collagenase Type 4: 0.15%
Deoxyribonuclease I: 0.02%
 RPMI 1640 Sharma N, Luo J, Kirschmann DA, O'Malley Y, Robbins ME, Akporiaye ET, Lubaroff DM, Heidger PM, Hendrix MJ: A novel immunological model for the study of prostate cancer, Cancer Res 59, 2271-6, 1999
Rat Rat (ACI/NMs X BUF/Mna) F1, male, 28 months, rat, (ACI/MNs) male, 8 weeks Epthelial Collagenase Type 3: 0.1%
 Eagle's MEM Serum-free Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus, In Vitro Cell Dev Biol 26, 713, 1990
Rat Rat Yolk sac tumor Trypsin: 0.01%
 DMEM Brennan, M., Oldberg, A., Hayman, E., and Ruoslahti, E.: Effect of a Proteoglycan Produced by Rat Tumor Cells on Their Adhesion to Fibronectin-Collagen Substrata, Cancer Res 43, 4302, 1983
Rat Rat, SD, female Epithelial, cancer and tumor Collagenase: 0.1%
 Eagles's MEM Cohen, L.: Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma, In Vitro 18, 565, 1982
Rat Rat Tumor, islet Trypsin: 0.05%
 Medium 199 Gazdar, A., Chick, W., Oie, H., Sims, H., King, D., Weir, G., and Lauris, V.: Continuous, Clonal, Insulin-and Somatostatin-Secreting Cell Lines Established from a Transplantable Rat Islet Cell Tumor, Proc Natl Acad Sci U S A 77 (6), 3519, 1980
Rat Rat Ascites hepatoma Trypsin: 0.1%
 Phosphate buffer (see reference) Essner, E.: Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands, Exp Cell Res 7, 430, 1954