Thyroid/Parathyroid - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine, adult Parathyroid Deoxyribonuclease I: 0.005%,
 HEPES Ham's F10 Nygren, P., Gylfe, E., Larsson, R., Johansson, H., Juhlin, C., Klareskoq, L., Akerstrom, G., and Rastad, J.: Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism, Biochim. Biophys. Acta 968, 253, 1988
Bovine Bovine Parathyroid glands Deoxyribonuclease I: 0.0075%
 HEPES buffer Wallace, J., and Scarpa, A.: Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations, J. Biol. Chem. 257, 10613, 1982
Bovine Bovine Parathyroid Deoxyribonuclease I: 0.004%
 Eagle's #2 medium without bicarbonate Brown, E., Hurwitz, S., and Aurbach, G.: Preparation of Viable Isolated Bovine Parathyroid Cells, Endocrinology 99, 1582, 1976
Bovine Bovine
(also porcine)		 Thyroid Trypsin: 0.004%
 EBSS Tong, W.: The Isolation and Culture of Thyroid Cells, Methods Enzymol. 32, 745, 1974
Chicken Chick embryo Thyroid
Muscle
Heart		 Collagenase: 0.25%
 Tyrode's saline, potassium free Hilfer, S., and Brown, J.: Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart, Exp. Cell Res. 65, 246, 1971
Chicken Chicken, Rhode Island Red, embryo Thyroid follicular Collagenase: 0.2%
 Tyrode's solution, CMF Spooner, B.: The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture, J. Cell. Physiol. 75, 33, 1970
Human Human thyroid Collagenase: 300 u/ml
 Ham's F-12/MEM Miller RC, Hiraoka T, Nakamura N, Tenou H, Kopecky KJ, Jones MP, Gould MN: In vitro culture of human thyroid cells; methods and application to radiation biology.,J Radiat Res (Tokyo) Vol. 26,, , 269-82, 1985
Porcine Porcine
(also bovine)		 Thyroid Trypsin: 0.004%
 EBSS Tong, W.: The Isolation and Culture of Thyroid Cells, Methods Enzymol. 32, 745, 1974
Sheep Sheep Thyroid Collagenase: 0.2%
 Puck's Saline F Kerkof, P.: Preparation of Primary Cultures of Ovine Thyroid Gland Cells, J. Tiss. Cult. Meth. 7, 23, 1982