Thyroid/Parathyroid - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine, adult Parathyroid Deoxyribonuclease I: 0.005%,
HEPES Ham's F10 Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism , Biochim Biophys Acta 968, 253, 1988
Bovine Bovine Parathyroid glands Deoxyribonuclease I: 0.0075%
HEPES buffer Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations , J Biol Chem 257, 10613, 1982
Bovine Bovine Parathyroid Deoxyribonuclease I: 0.004%
Eagle's #2 medium without bicarbonate Preparation of Viable Isolated Bovine Parathyroid Cells , Endocrinology 99, 1582, 1976
Bovine Bovine
(also porcine)
Thyroid Trypsin: 0.004%
EBSS The Isolation and Culture of Thyroid Cells , Methods Enzymol 32, 745, 1974
Chicken Chick embryo Thyroid Muscle Heart Collagenase: 0.25%
Tyrode's saline, potassium free Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart , Exp Cell Res 65, 246, 1971
Chicken Chicken, Rhode Island Red, embryo Thyroid follicular Collagenase: 0.2%
Tyrode's solution, CMF The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture , J Cell Physiol 75, 33, 1970
Porcine Porcine
(also bovine)
Thyroid Trypsin: 0.004%
EBSS The Isolation and Culture of Thyroid Cells , Methods Enzymol 32, 745, 1974
Sheep Sheep Thyroid Collagenase: 0.2%
Puck's Saline F , J Tiss Cul Meth 7, 23, 1982