Stem - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Human Human, male spermatogonial stem cells Collagenase: 1%
Deoxyribonuclease I: 0.22%
Trypsin: 0.4%
 DMEM Kossack Nina, Meneses Juanito, Shefi Shai, Nguyen Ha Nam, Chavez Shawn, Nicholas Cory, Gromoll Joerg, Turek Paul J, Reijo-Pera Renee A: Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells., Stem Cells , , 2008
Human Human Bone marrow derived MSC Trypsin: 0.05%
Papain: 0.0025%
 DMEM Welter Jean F, Solchaga Luis A, Penick Kitsie J: Simplification of aggregate culture of human mesenchymal stem cells as a chondrogenic screening assay, Biotechniques 42, 732, 734-7, 2007
Human Human, 8-12 year Tendon stem/progenitor Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche, Nat Med 13, 1219-27, 2007
Human Human, adult Adipose-derived adult stem Collagenase Type 1: 0.1%
 DMEM/F-12 Ham's Mitchell James B, McIntosh Kevin, Zvonic Sanjin, Garrett Sara, Floyd Z Elizabeth, Kloster Amy, Di Halvorsen Yuan, Storms Robert W, Goh Brian, Kilroy Gail, Wu Xiying, Gimble Jeffrey M: Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers, Stem Cells 24, 376-85, 2006
Human Human Mesenchymal stem Collagenase Type 1: 0.075%
 DMEM Kern Susanne, Eichler Hermann, Stoeve Johannes, Kluter Harald, Bieback Karen: Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue, Stem Cells 24, 1294-301, 2006
Human Human Placental mesenchymal stem Collagenase Type 2: 270 u/ml
Neutral Protease: 2.4 u/ml
 MEM Portmann-Lanz CBettina, Schoeberlein Andreina, Huber Alexander, Sager Ruth, Malek Antoine, Holzgreve Wolfgang, Surbek DanielV: Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration, Am J Obstet Gynecol 194, 664-73, 2006
Human Human Stromal stem cells Collagenase Type 1: 0.2%
 HBSS Boquest Andrew C, Shahdadfar Aboulghassem, Brinchmann Jan E, Collas Philippe: Isolation of stromal stem cells from human adipose tissue, Methods Mol Biol 325, 35-46, 2006
Human Human, adult, male Human epidermal keratinocyte stem cells Neutral Protease: 0.5%
 DMEM Papini S, Cecchetti D, Campani D, Fitzgerald W, Grivel J, Chen S, Margolis L, Revoltella R.: Isolation and Clonal Analysis of Human Epidermal Keratinocyte Stem Cells in Long-term Culture, Stem Cells 21, 481, 2003
Human Human Umbilical vein mesenchymal stem cells Collagenase: 1%
 PBS Covas DT, Siufi JL C, Silva AR L, Orellana MD: Isolation and culture of umbilical vein mesenchymal stem cells, Braz J Med Biol Res 36, 1179-83, 2003
Human Human, 7-8 yr Stem cells Human exfoliated deciduous teeth Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 see reference Miura, M., Gronthos, S, Zhao, M, Lu, B, Fisher, L, Robey, P, and Shi, S.: SHED: Stem Cells from Human Exfoliated Deciduous Teeth, Proc Natl Acad Sci U S A 100, 5807, 2003
Human Human, adult Human skin mast cells Collagenase Type 2: 0.15%
Hyaluronidase: 0.07%
Deoxyribonuclease I: 0.03%
 HBSS Kambe, N, Kambe, M, Kochan, J, and Schwartz ,L.: Human Skin-derived Mast Cells Can Proliferate While Retaining Their Characteristic Functional and Protease Phenotypes, Blood 97, 2045, 2001
Human Human Embryonic stem Neutral Protease: 1%
Collagenase Type 4: 0.1%
 DMEM Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts, Science 282, 1145-7, 1998
Human Human Muscle-derived stem cells Trypsin: 0.25%
 DMEM/F12 Alessandri Giulio, Pagano Stefano, Bez Alessandra, Benetti Anna, Pozzi Stefano, Iannolo Gioacchin, Baronio Manuela, Invernici Gloria, Caruso Arnaldo, Muneretto Claudio, Bisleri Gianluigi, Parati Eugenio: Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages, Lancet 364, 1872-83,
Monkey Monkey Embryonic stem Collagenase Type 4: 0.08%
 DMEM Chen, S., Revoltella, R., Papini, S., Michelini, M., Fitzgerald, W., Zimmerberg, J.,and Margolis, L.: Multilineage Differentiation of Rhesus Monkey Embryonic Stem cells in Three-dimensional Culture Systems, Stem Cells 21(3), 281, 2003
Monkey Baboons, 1yr, 7yr, 14 yr Primate spermatogonial Collagenase Type 2: 0.1%
Trypsin: 0.05%
Deoxyribonuclease I: 0.1%
 DMEM Nagano, M., McCarrey, J., and Brinster, R.: Primate Spermatogonial Stem Cells Colonize Mouse Testes, Biol Reprod 64, 1409, 2001
Mouse Mouse, embryonic HES-BC cells Trypsin: 0.05%
 DMEM Lu Shi-Jiang, Feng Qiang, Caballero Sergio, Chen Yu, Moore Malcolm A, Grant Maria B, Lanza Robert: Generation of functional hemangioblasts from human embryonic stem cells, Nat Methods 4, 501-9, 2007
Mouse Mouse, 6-8 week Tendon stem/progenitor Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche, Nat Med 13, 1219-27, 2007
Mouse Mouse Liver epithelial progenitor cells Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.05%
 DMEM Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping: Isolation and characterization of bipotent liver progenitor cells from adult mouse, Stem Cells 24, 322-32, 2006
Mouse Mouse, 6 week Adipose mesenchymal stem Collagenase: 0.2%
 PBS Di Rocco Giuliana, Iachininoto Maria Grazia, Tritarelli Alessandra, Straino Stefania, Zacheo Antonella, Germani Antonia, Crea Filippo, Capogrossi Maurizio C: Myogenic potential of adipose-tissue-derived cells, J Cell Sci 119, 2945-52, 2006
Mouse Mouse, day 7 Cerebellar stem cells Papain: 10u/ml
Deoxyribonuclease I: 250 u/ml
 Dulbecco's PBS Lee, A, Kessler, J, Read, T, Kaiser, C, Corbeil, D, Huttner ,W, Johnson, J, Wechsler-Reya, R.: Isolation of Neural Stem Cells from the Postnatal Cerebellum, Nat Neurosci 8, 723, 2005
Mouse Mouse, embryonic Embryonic fibroblast feeder cells Collagenase Type 4: 0.1%
 DMEM Schatten Gerald, Smith Joseph, Navara Christopher, Park Jong-Hyuk, Pedersen Roger: Culture of human embryonic stem cells, Nat Methods 2, 455-63, 2005
Mouse Mouse, 1 day neural progenitor PDS kit: per instructions
 see reference Seaberg Raewyn M, Smukler Simon R, Van der Kooy Derek: Intrinsic differences distinguish transiently neurogenic progenitors from neural stem cells in the early postnatal brain., Dev Biol 278, 71-85, 2005
Mouse Mouse, 15 day or 3 month Neural stem cells Papain: 0.1%
 DMEM/F-12 Gritti, A, Bonfanti, L, Doetsch, F, Caille, I, Alvarez-Buylla, A, Lim, D, Galli, R, Verdugo J, Herrera, D, and Vescovi A.: Multipotent Neural Stem Cells Reside into the Rostral Extension and Olfactory Bulb of Adult Rodents, J Neurosci 22(2), 437, 2002
Mouse Mouse, 4-8 month Neural subventricular zone Trypsin: 0.13%
Hyaluronidase: 0.067%
 DMEM/F12 Gritti A, Frolichsthal-Schoeller P, Galli R, Parati E, Cova L, Pagano S, Bjornson C, and Vescovi A.: Epidermal and Fibroblast Growth Factors Behave as Mitogenic Regulators for a Single Multipotent Stem Cell-like Population from the Subventricular Region of the Adult Mouse forebrain, J Neurosci 19(9), 3287, 1999
Mouse Mouse, 6 week Muscle hematopoietic stem cells Collagenase: 0.2%
Trypsin: 0.1%
 DMEM Jackson, K., and Goodell, M.: Hematopoietic Potential of Stem Cells Isolated from Murine Skeletal Muscle, Proc Natl Acad Sci U S A 96, 14482, 1999
Porcine Porcine, female, <1 year adipose mesenchymal stems cells Collagenase Type 1: 0.1%
 DMEM Williams KellieJ, Picou AliciaA, Kish SharonL, Giraldo AngelicaM, Godke RobertA, Bondioli KennethR: Isolation and characterization of porcine adipose tissue-derived adult stem cells., Cells Tissues Organs 188, 251-8, 2008
Rat Rat, SD, newborn Neutral stem cells, Schwann cells Trypsin: 0.25%
Collagenase: 0.16%
 DMEM/F12 Zeng Yuan-Shan, Ding Ying, Wu Li-Zhi, Guo Jia-Song, Li Hai-Biao, Wong Wai-Man, Wu Wu-Tian: Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord, Dev Neurosci 27, 20-6, 2005
Rat Rat, male, 200-250 g Neural stem cells Papain: 0.09%
Deoxyribonuclease I: 0.1%
 EBSS Gobbel GT, Choi SJ, Beier S, Niranjan A: Long-term cultivation of multipotential neural stem cells from adult rat subependyma, Brain Res 980, 221, 2003
Rat Rat, adult Neural stem cells Papain: 2.5 u/ml
Deoxyribonuclease I: 250 u/ml
Neutral Protease: 1 u/ml
 DMEM/F-12 Palmer, T., Markakis, E., Willhoite, A., Safar, F., and Gage, F.: Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS, J Neurosci 19, 8487, 1999