Stem - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Equine Equine, 1-5 year Adipose derived stem Collagenase Type 1: 0.1
 PBS Vidal Martin A, Kilroy Gail E, Lopez Mandi J, Johnson Jill R, Moore Rustin M, and Gimble Jeffrey M: Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells., Vet Surg 36, 613-22, 2007
Human Human Glioma Stem Cells PDS kit: per instructions
 Neurobasal medium Hjelmeland, A, Wu, Q, Wickman, S, Eyler, C, Heddleston, J, Shi, Q, Lathia, J, Macswords, J, Lee, J, McLendon, R and Rich, J: Targeting A20 decreases glioma stem cell survival and tumor growth., P.L.o.S. ONE 8, e1000319, 2010
Human Human, 40-65 year Adult human adipose stem cells Collagenase Type 2: 0.075%
 DMEM Sun Ning, Panetta Nicholas J, Gupta Deepak M, Wilson Kitchener D, Lee Andrew, Jia Fangjun, Hu Shijun, Cherry Athena M, Robbins Robert C, Longaker Michael T and Wu Joseph C: Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells., Proc. Natl. Acad. Sci. USA 106, 15720-5, 2009
Human Human Filum terminale neural progenitor Trypsin: see reference
 DMEM/F-12 Varghese, M, Olstorn, H, Berg-Johnsen, J, Moe, M.C, Murrell W and Langmoen I: Isolation of human multipotent neural progenitors from adult filum terminale., Stem Cell Dev 18, 603-13, 2009
Human Human, male Spermatogonial stem cells Collagenase: 1%
Deoxyribonuclease I: 0.22%
Trypsin: 0.4%
 DMEM Kossack Nina, Meneses Juanito, Shefi Shai, Nguyen Ha Nam, Chavez Shawn, Nicholas Cory, Gromoll Joerg, Turek Paul J, Reijo-Pera Renee A: Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells., Stem Cells , , 2008
Human Human Adipose derived stem Collagenase Type 1: 0.075%
 DMEM Jeong, Jae Ho: Adipose Stem Cells as a Clinically Available and Effective Source of Adult Stem Cell Therapy, Int J Stem Cells 1, 43, 2008
Human Human Bone marrow derived MSC Trypsin: 0.05%
Papain: 0.0025%
 DMEM Welter Jean F, Solchaga Luis A, Penick Kitsie J: Simplification of aggregate culture of human mesenchymal stem cells as a chondrogenic screening assay, Biotechniques 42, 732, 734-7, 2007
Human Human, 8-12 year Tendon stem/progenitor Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche, Nat. Med. 13, 1219-27, 2007
Human Human, male Adipose derived adult stem cells Collagenase Type 1: 0.1%
 DMEM Lei Lei, Liao WeiMing, Sheng PuYi, Fu Ming, He AiShan, Huang Gang: Biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture., Sci. China C 50, 320-8, 2007
Human Adipose derived stromal cells Collagenase Type 1: 0.15%
 DMEM Schaffler A, Buchler C: Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies, Stem Cells 25, 818-27, 2007
Human Human, adult Adipose-derived adult stem Collagenase Type 1: 0.1%
 DMEM/F-12 Mitchell James B, McIntosh Kevin, Zvonic Sanjin, Garrett Sara, Floyd Z Elizabeth, Kloster Amy, Di Halvorsen Yuan, Storms Robert W, Goh Brian, Kilroy Gail, Wu Xiying, Gimble Jeffrey M: Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers, Stem Cells 24, 376-85, 2006
Human Human Mesenchymal stem Collagenase Type 1: 0.075%
 DMEM Kern Susanne, Eichler Hermann, Stoeve Johannes, Kluter Harald, Bieback Karen: Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue, Stem Cells 24, 1294-301, 2006
Human Human Placental mesenchymal stem Collagenase Type 2: 270 u/ml
Neutral Protease: 2.4 u/ml
 MEM Portmann-Lanz CBettina, Schoeberlein Andreina, Huber Alexander, Sager Ruth, Malek Antoine, Holzgreve Wolfgang, Surbek DanielV: Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration, Am. J. Obstet. Gynecol. 194, 664-73, 2006
Human Human Stromal stem cells Collagenase Type 1: 0.2%
 HBSS Boquest Andrew C, Shahdadfar Aboulghassem, Brinchmann Jan E, Collas Philippe: Isolation of stromal stem cells from human adipose tissue, Methods Mol. Biol. 325, 35-46, 2006
Human Human, female Adipose-derived adult stem Collagenase Type 1: 0.1%
 DMEM/F-12 Guilak Farshid, Lott Kristen E, Awad Hani A, Cao Qiongfang, Hicok Kevin C, Fermor Beverley, Gimble Jeffrey M: Clonal analysis of the differentiation potential of human adipose-derived adult stem cells., J. Cell. Physiol. 206, 229-37, 2006
Human Human Mesenchymal stem Collagenase Type 1: 0.1%
 HBSS Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho: Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK, J. Lipid Res. 47, 653-64, 2006
Human Human Adipocytes, stromal vascular Collagenase: 0.2%
 HBSS Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E: Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture, Mol. Biol. Cell 16, 1131-41, 2005
Human Human Multipotent adipose derived stem Collagenase: 0.2%
 DMEM Rodriguez Anne-Marie, Pisani Didier, Dechesne Claude A, Turc-Carel Claude, Kurzenne Jean-Yves, Wdziekonski Brigitte, Villageois Albert, Bagnis Claude, Breittmayer Jean-Philippe, Groux Herve, Ailhaud Gerard, Dani Christian: Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse, J. Exp. Med. 201, 1397-405, 2005
Human Human, female, 34-51 yr Endometrial epithelial and stromal cells Collagenase Type 3: 0.03%
Deoxyribonuclease I: 0.004%
 DMEM/F-12 Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E: Clonogenicity of human endometrial epithelial and stromal cells, Biol. Reprod. 70, 1738-50, 2004
Human Human Mesenchymal Collagenase: 0.2%
Trypsin: 2.5%
 DMEM Wang, H., Hung, S., Peng, S., Huang, C., Wei, H., Guo, Y., Fu, Y.,Lai, M. and Chen, C: Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord, Stem Cells 22, 1330, 2004
Human Human, female Adipose derived adult stem cells Collagenase Type 1: 0.1%
 DMEM/F-12 Aust L, Devlin B, Foster SJ, Halvorsen YD C, Hicok K, du Laney T, Sen A, Willingmyre GD, Gimble JM: Yield of human adipose-derived adult stem cells from liposuction aspirates.,Cytotherapy Vol. 6,, , 7-14, 2004
Human Human Stromal vascular, adipocytes, stem Collagenase: 300 u/ml
 PBS Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells, Circulation 110, 349-55, 2004
Human Human, adult, male Human epidermal keratinocyte stem cells Neutral Protease: 0.5%
 DMEM Papini S, Cecchetti D, Campani D, Fitzgerald W, Grivel J, Chen S, Margolis L, Revoltella R.: Isolation and Clonal Analysis of Human Epidermal Keratinocyte Stem Cells in Long-term Culture, Stem Cells 21, 481, 2003
Human Human Umbilical vein mesenchymal stem cells Collagenase: 1%
 PBS Covas DT, Siufi JL C, Silva AR L, Orellana MD: Isolation and culture of umbilical vein mesenchymal stem cells, Braz. J. Med. Biol. Res. 36, 1179-83, 2003
Human Human, 7-8 yr Stem cells Human exfoliated deciduous teeth Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 see reference Miura, M., Gronthos, S, Zhao, M, Lu, B, Fisher, L, Robey, P, and Shi, S.: SHED: Stem Cells from Human Exfoliated Deciduous Teeth, Proc. Natl. Acad. Sci. USA 100, 5807, 2003
Human Human Processed lipoaspirate cells Collagenase Type 1: 0.075%
 PBS Zuk Patricia A, Zhu Min, Ashjian Peter, De Ugarte Daniel A, Huang Jerry I, Mizuno Hiroshi, Alfonso Zeni C, Fraser JohnK, Benhaim Prosper, Hedrick Marc H: Human adipose tissue is a source of multipotent stem cells, Mol. Biol. Cell 13, 4279-95, 2002
Human Human, adult Human skin mast cells Collagenase Type 2: 0.15%
Hyaluronidase: 0.07%
Deoxyribonuclease I: 0.03%
 HBSS Kambe, N, Kambe, M, Kochan, J, and Schwartz ,L.: Human Skin-derived Mast Cells Can Proliferate While Retaining Their Characteristic Functional and Protease Phenotypes, Blood 97, 2045, 2001
Human Human Central nervous system stem Collagenase: 0.1%
Hyaluronidase: 0.1%
 HBSS Uchida N, Buck D W, He D, Reitsma M J, Masek M, Phan T V, Tsukamoto A S, Gage F H, and Weissman I L: Direct isolation of human central nervous system stem cells., Proc. Natl. Acad. Sci. USA 97, 14720-5, 2000
Human Human Embryonic stem Neutral Protease: 1%
Collagenase Type 4: 0.1%
 DMEM Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts, Science 282, 1145-7, 1998
Human Human Muscle-derived stem cells Trypsin: 0.25%
 DMEM/F-12 Alessandri Giulio, Pagano Stefano, Bez Alessandra, Benetti Anna, Pozzi Stefano, Iannolo Gioacchin, Baronio Manuela, Invernici Gloria, Caruso Arnaldo, Muneretto Claudio, Bisleri Gianluigi, Parati Eugenio: Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages, Lancet 364, 1872-83,
Monkey Monkey Embryonic stem Collagenase Type 4: 0.08%
 DMEM Chen, S., Revoltella, R., Papini, S., Michelini, M., Fitzgerald, W., Zimmerberg, J.,and Margolis, L.: Multilineage Differentiation of Rhesus Monkey Embryonic Stem cells in Three-dimensional Culture Systems, Stem Cells 21(3), 281, 2003
Monkey Baboons, 1yr, 7yr, 14 yr Primate spermatogonial Collagenase Type 2: 0.1%
Trypsin: 0.05%
Deoxyribonuclease I: 0.1%
 DMEM Nagano, M., McCarrey, J., and Brinster, R.: Primate Spermatogonial Stem Cells Colonize Mouse Testes, Biol. Reprod. 64, 1409, 2001
Mouse Mouse, adult Adult neutral stem Trypsin: 0.05%
 DMEM Deleyrolle Loic P, Reynolds Brent A: Isolation, expansion, and differentiation of adult Mammalian neural stem and progenitor cells using the neurosphere assay.,Methods Mol Biol Vol. 549,, , 91-101, 2009
Mouse Mouse Germ cells Collagenase: 100 u/ml
 PBS Breault David T, Min Irene M, Carlone Diana L, Farilla Loredana G, Ambruzs Dana M, Henderson Daniel E, Algra Selma, Montgomery Robert K, Wagers Amy J and Hole Nicholas: Generation of mTert-GFP mice as a model to identify and study tissue progenitor cells., Proc. Natl. Acad. Sci. USA 105, 10420-5, 2008
Mouse Mouse Neural progenitor cell Papain: 10ul/ml
 PBS Hutton, S and Pevny, L: Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System, Cold Spring Harb. Protoc. 11, 5077, 2008
Mouse Mouse, embryonic HES-BC cells Trypsin: 0.05%
 DMEM Lu Shi-Jiang, Feng Qiang, Caballero Sergio, Chen Yu, Moore Malcolm A, Grant Maria B, Lanza Robert: Generation of functional hemangioblasts from human embryonic stem cells, Nat. Methods 4, 501-9, 2007
Mouse Mouse, 6-8 week Tendon stem/progenitor Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche, Nat. Med. 13, 1219-27, 2007
Mouse Mouse mesenchymal stem Collagenase: 0.2%
 DMEM Gang, E., Bosnakovski, D., Figueiredo, J., Visser, J. and Perlingeiro, R: SSEA-4 identifies mesenchymal stem cells from bone marrow, Blood 109, 1743, 2007
Mouse Mouse Liver epithelial progenitor cells Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.05%
 DMEM Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping: Isolation and characterization of bipotent liver progenitor cells from adult mouse, Stem Cells 24, 322-32, 2006
Mouse Mouse, 6 week Adipose mesenchymal stem Collagenase: 0.2%
 PBS Di Rocco Giuliana, Iachininoto Maria Grazia, Tritarelli Alessandra, Straino Stefania, Zacheo Antonella, Germani Antonia, Crea Filippo, Capogrossi Maurizio C: Myogenic potential of adipose-tissue-derived cells, J. Cell Sci. 119, 2945-52, 2006
Mouse Mouse neutral stem cells Papain: see reference
 DMEM/F-12 Meletis, K, Wirta, V, Hede, S, Nister, M, Lundeberg, J, and Frisen, J.: p53 suppresses the self-renewal of adult neural stem cells., Development 133, 363-9, 2006
Mouse Mouse, day 7 Cerebellar stem cells Papain: 10u/ml
Deoxyribonuclease I: 250 u/ml
 Dulbecco's PBS Lee, A, Kessler, J, Read, T, Kaiser, C, Corbeil, D, Huttner ,W, Johnson, J, Wechsler-Reya, R.: Isolation of Neural Stem Cells from the Postnatal Cerebellum, Nat. Neurosci. 8, 723, 2005
Mouse Mouse, embryonic Embryonic fibroblast feeder cells Collagenase Type 4: 0.1%
 DMEM Schatten Gerald, Smith Joseph, Navara Christopher, Park Jong-Hyuk, Pedersen Roger: Culture of human embryonic stem cells, Nat. Methods 2, 455-63, 2005
Mouse Mouse, 1 day neural progenitor PDS kit: per instructions
 see reference Seaberg Raewyn M, Smukler Simon R, Van der Kooy Derek: Intrinsic differences distinguish transiently neurogenic progenitors from neural stem cells in the early postnatal brain., Dev. Biol. 278, 71-85, 2005
Mouse Mouse, 8-10 week liver derived stem cells Collagenase Type 1: 0.1%
Neutral Protease: 2.4 u/ml
 HBSS Kotton Darrell N, Fabian Attila J, Mulligan Richard C: A novel stem-cell population in adult liver with potent hematopoietic-reconstitution activity., Blood 106, 1574-80, 2005
Mouse Mouse bone marrow Collagenase Type 1: 0.15%
Neutral Protease: 0.15%
 PBS Bertoncello, Ivan and Williams, Brenda: Hematopoietic stem cell characterization by Hoechst 33342 and rhodamine 123 staining., Methods Mol. Biol. 263, 181-200, 2004
Mouse Mouse, 4-7 day pluripotent stem cells Collagenase Type 1: 220 u/ml
Neutral Protease: 33 u/ml
 MEM Howell Jonathan C, Lee Wei-Hua, Morrison Paul, Zhong Jin, Yoder Mervin C, Srour Edward F: Pluripotent stem cells identified in multiple murine tissues., Ann. NY Acad. Sci. 996, 158-73, 2003
Mouse Mouse, 15 day or 3 month Neural stem cells Papain: 0.1%
 DMEM/F-12 Gritti, A, Bonfanti, L, Doetsch, F, Caille, I, Alvarez-Buylla, A, Lim, D, Galli, R, Verdugo J, Herrera, D, and Vescovi A.: Multipotent Neural Stem Cells Reside into the Rostral Extension and Olfactory Bulb of Adult Rodents, J. Neurosci. 22(2), 437, 2002
Mouse Mouse, 4-8 month Neural subventricular zone Trypsin: 0.13%
Hyaluronidase: 0.067%
 DMEM/F12 Gritti A, Frolichsthal-Schoeller P, Galli R, Parati E, Cova L, Pagano S, Bjornson C, and Vescovi A.: Epidermal and Fibroblast Growth Factors Behave as Mitogenic Regulators for a Single Multipotent Stem Cell-like Population from the Subventricular Region of the Adult Mouse forebrain, J. Neurosci. 19(9), 3287, 1999
Mouse Mouse, 6 week Muscle hematopoietic stem cells Collagenase: 0.2%
Trypsin: 0.1%
 DMEM Jackson, K., and Goodell, M.: Hematopoietic Potential of Stem Cells Isolated from Murine Skeletal Muscle, Proc. Natl. Acad. Sci. USA 96, 14482, 1999
Porcine Porcine, female, <1 year adipose mesenchymal stems cells Collagenase Type 1: 0.1%
 DMEM Williams KellieJ, Picou AliciaA, Kish SharonL, Giraldo AngelicaM, Godke RobertA, Bondioli KennethR: Isolation and characterization of porcine adipose tissue-derived adult stem cells., Cells Tissues Organs 188, 251-8, 2008
Rat Rat, SD, newborn Neutral stem cells, Schwann cells Trypsin: 0.25%
Collagenase: 0.16%
 DMEM/F12 Zeng Yuan-Shan, Ding Ying, Wu Li-Zhi, Guo Jia-Song, Li Hai-Biao, Wong Wai-Man, Wu Wu-Tian: Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord, Dev. Neurosci. 27, 20-6, 2005
Rat Rat, male, 200-250 g Neural stem cells Papain: 0.09%
Deoxyribonuclease I: 0.1%
 EBSS Gobbel GT, Choi SJ, Beier S, Niranjan A: Long-term cultivation of multipotential neural stem cells from adult rat subependyma, Brain Res. 980, 221, 2003
Rat Rat, adult Neural stem cells Papain: 2.5 u/ml
Deoxyribonuclease I: 250 u/ml
Neutral Protease: 1 u/ml
 DMEM/F-12 Palmer, T., Markakis, E., Willhoite, A., Safar, F., and Gage, F.: Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS, J. Neurosci. 19, 8487, 1999
Rat Rat central nervous system stem cells Papain: 0.01%
Neutral Protease: 0.1%
 DMEM/F-12 Ray, J and Gage, F: Neural Stem Cell Isolation, Characterization and Transplantation,Modern Techniques in Neuroscience Research,Johansson, H and Windhorst, U, Springer-Verlag New York, 339-360, 1999
Rat Rat, embryonic neurons and progenitor Papain: 20 u/ml
 EBSS Maric D, Maric I, Barker JL: Buoyant density gradient fractionation and flow cytometric analysis of embryonic rat cortical neurons and progenitor cells.,Methods Vol. 16,, , 247-59, 1998