Stem - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Human human placental mesenchymal stem Collagenase Type 2: 270 u/ml
Dispase: 2.4 u/ml
 MEM Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration. , Am J Obstet Gynecol Vol. 194,, , 664-73, 2006
Human adult adipose-derived adult stem Collagenase Type 1: 0.1%
 DMEM/F-12 Ham's Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers. , Stem Cells 24, 376-85, 2006
Human 7-8yr stem cells Human exfoliated deciduous teeth Collagenase Type 1: 0.3%
Dispase: 0.4%
 see reference SHED: Stem Cells from Human Exfoliated Deciduous Teeth , Proc Natl Acad Sci U S A 100, 5807, 2003
Human adult, male human epidermal keratinocyte stem cells Dispase: 0.5%
 DMEM Isolation and Clonal Analysis of Human Epidermal Keratinocyte Stem Cells in Long-term Culture , Stem Cells 21, 481, 2003
Human human umbilical vein mesenchymal stem cells Collagenase: 1%
 PBS Isolation and culture of umbilical vein mesenchymal stem cells. , Braz J Med Biol Res Vol. 36,, , 1179-83, 2003
Human adult human skin mast cells Collagenase Type 2: 0.15%
Hyaluronidase: 0.07%
Deoxyribonuclease I: 0.03%
 HBSS Human Skin-derived Mast Cells Can Proliferate While Retaining Their Characteristic Functional and Protease Phenotypes , Blood 97, 2045, 2001
Monkey monkey embryonic stem Collagenase Type 4: 0.08%
 DMEM Multilineage Differentiation of Rhesus Monkey Embryonic Stem cells in Three-dimensional Culture Systems , Stem Cells 21(3), 281, 2003
Monkey baboons, 1yr, 7yr, 14 yr primate spermatogonial Collagenase Type 2: 0.1%
Trypsin: 0.05%
Deoxyribonuclease I: 0.1%
 DMEM Primate Spermatogonial Stem Cells Colonize Mouse Testes , Biol Reprod 64, 1409, 2001
Mouse embryonic hES-BC cells Trypsin: 0.05%
 DMEM Generation of functional hemangioblasts from human embryonic stem cells. , Nat Methods Vol. 4,, , 501-9, 2007
Mouse postnatal day 7 cerebellar stem cells Papain: 10u/ml
Deoxyribonuclease I: 250 u/ml
 Dulbecco's PBS Isolation of Neural Stem Cells from the Postnatal Cerebellum , Nat Neurosci 8, 723, 2005
Mouse mouse, embryonic embryonic fibroblast feeder cells Collagenase Type 4: 0.1%
 DMEM Culture of human embryonic stem cells. , Nat Methods Vol. 2,, , 455-63, 2005
Mouse 15 day or 3 month neural stem cells Papain: 0.1%
 DMEM F-12 Multipotent Neural Stem Cells Reside into the Rostral Extension and Olfactory Bulb of Adult Rodents , J Neurosci 22(2), 437, 2002
Mouse 6 week muscle hematopoietic stem cells Collagenase: 0.2%
Trypsin: 0.1%
 DMEM Hematopoietic Potential of Stem Cells Isolated from Murine Skeletal Muscle , Proc Natl Acad Sci U S A 96, 14482, 1999
Mouse 4-8 month neural subventricular zone Trypsin: 0.13%
Hyaluronidase: 0.067%
 DMEM/F12 Epidermal and Fibroblast Growth Factors Behave as Mitogenic Regulators for a Single Multipotent Stem Cell-like Population from the Subventricular Region of the Adult Mouse forebrain. , J Neurosci 19(9), 3287, 1999
Rat SD, newborn neutral stem cells, Schwann cells Trypsin: 0.25%
Collagenase: 0.16%
 DMEM/F12 Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord. , Dev Neurosci 27, 20-6, 2005
Rat rat, male, 200-250 g neural stem cells Papain: 0.09%
Deoxyribonuclease I: 0.1%
 EBSS Long-term cultivation of multipotential neural stem cells from adult rat subependyma. , Brain Res 980, 221, 2003
Rat adult neural stem cells Papain: 2.5 u/ml
Deoxyribonuclease I: 250 u/ml
Neutral Protease: 1 u/ml
 DMEM/F-12 Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS. , J Neurosci 19, 8487, 1999