Spleen - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Mouse Mouse dentritic Collagenase: 300 u/ml
Deoxyribonuclease I: .002%
 RPMI 1640 Abe Kazumichi, Nguyen KimPhung, Fine SeanD, Mo Ji-Hun, Shen Carol, Shenouda Steve, Corr Maripat, Jung Steffen, Lee Jongdae, Eckmann Lars, Raz Eyal: Conventional dendritic cells regulate the outcome of colonic inflammation independently of T cells., Proc. Natl. Acad. Sci. USA 104, 17022-7, 2007
Mouse Mouse Splenic stromal Collagenase Type 3: 100-400 u/ml
 HBSS Benedict Chris A, De Trez Carl, Schneider Kirsten, Ha Sukwon, Patterson Ginelle, Ware Carl F: Specific remodeling of splenic architecture by cytomegalovirus, P.L.o.S. Pathog. 2, e16, 2006
Mouse Mouse, 4-6 week Dentritic Collagenase Type 1: 0.1%
Deoxyribonuclease I: 0.001%
 RPMI-1640 McLellan AlexanderD, Kapp Michaela, Eggert Andreas, Linden Christian, Bommhardt Ursula, Bröcker Eva-B, Kämmerer Ulrike, Kämpgen Eckhart: Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression, Blood 99, 2084-93, 2002
Mouse Mouse Dentritic Collagenase Type 3: 0.1%
Deoxyribonuclease I: 325 u/ml
 RPMI 1640 Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells, J. Exp. Med. 196, 1415, 2002
Mouse Mouse Dentritic Collagenase: 100 u/ml
 HBSS Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures, Blood 96, 3029-39, 2000