Skin - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Frog Frog Epidermal Trypsin: 0.18%
 Barth's solution, CMF Isolation, characterization, and in vitro culture of larval and adult epidermal cells of the frog Xenopus laevis. , In Vitro Cell Dev Biol 26, 1128, 1990
Human human, neonatal keratinocytes Dispase: 0.4%
Collagenase: 0.3%
 DMEM Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny , J Clin Invest 113, 390-400, 2004
Human Human mast cells Dispase: 0.05%
Collagenase Type 4: 1.0%
 RPMI Comparative cytokine profile of human skin mast cells from two compartments--strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming. , J Leukoc Biol Vol. 75,, , 244-52, 2004
Human Human, adult Keratinocytes Dispase: 0.25%
 PBS The Tetraspanin CD9 Associates with the Integrin a6B4 in Cultured Human Epidermal Keratinocytes and is Involved in Cell Motility , Eur J Cell Biol 79, 41, 2000
Human Human keratinocytes Dispase: 0.25%
Trypsin: 0.17%
Thermolysin: 0.05%
 see reference Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation. , Exp Dermatol Vol. 8,, , 30-8, 1999
Human Human human skin mast cells Dispase: 0.1%
Collagenase Type 1: see reference
 PBS Synthesis, storage, and release of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) by human mast cells: implications for the biological significance of VEGF206. , Mol Biol Cell 9, 875-84, 1998
Human human epidermal Collagenase Type 2: 0.1%
Dispase: 0.5-1.0%
 PBS HIV-1 selection by epidermal dendritic cells during transmission across human skin. , J Exp Med 187, 1623-31, 1998
Human Human Keratinocytes Trypsin: 0.05%
 Dulbecco's PBS Culture of Human Keratinocytes in Defined Serum-Free Medium, Focus 19 (1), 2, 1997
Human Human Epidermis plus dermis Abdomen or inner forearm Trypsin: 0.125%
 DMEM, M199 , Methods in Molecula Biology, Basic Cell Culture Protocols, 2nd ed. Vol. 75,Pollard, J., and Walker, J., Humana Press, 23, 1997
Human Human Keratinocytes Trypsin: 0.25%
 DMEM , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons Ltd, 11B:4.1, 1995
Human Human, adult, female, breast skin Keratinocytes Trypsin: 0.25%
 PBS Expression of Differentiation Markers in Human Adult Keratinocytes Cultured in Submerged Conditions , In Vitro Cell Dev Biol 30A, 372, 1994
Human Human, neonatal foreskin Fibroblasts Trypsin: 0.25%
 DMEM Evaluation of a Biodegradable Matrix Containing Cultured Human Fibroblasts as a Dermal Replacement Beneath Meshed Skin Grafts on Athymic Mice , Surgery 111 (1), 438, 1992
Human Human, 18-50 years Sweat duct Collagenase: 0.03%
 MEM Effects of Media Buffer Systems on Growth and Electrophysiologic Characteristics of Cultured Sweat Duct Cells , In Vitro Cell Dev Biol 27, 47, 1991
Human Human Fibroblasts Trypsin: 0.25%
 CMF solution Postmitotic Human Dermal Fibroblasts Preserve Intact Feeder Properties for Epithelial Cell Growth After Long-Term Cryopreservation , In Vitro Cell Dev Biol 26, 709, 1990
Human Human Sweat gland Collagenase Type 2: 0.015%
 (see reference) Ion Channels in Normal Human and Cystic Fibrosis Sweat Gland Cells , Am J Physiol 257, C129, 1989
Human Human, ages 16-30 Human sweat duct Collagenase Type 2: 0.2%
 RPMI 1640
(see reference)		 Human Sweat Duct Cells in Primary Culture. Basic Bioelectric Properties of Cultures Derived From Normals and Patients with Cystic Fibrosis , In Vitro Cell Dev Biol 25 (4), 342, 1989
Human Human Melanocytes, skin/ foreskin Trypsin: 0.25%
 PBS Induction of Nerve Growth Factor Receptors on Cultured Human Melanocytes , Proc Natl Acad Sci U S A 85, 5282, 1988
Human Human Human sweat glands Collagenase: 0.2%
 HBSS The Primary Culture of Epithelia From the Secretory Coil and Collecting Duct of Normal Human and Cystic Fibrotic Eccrine Sweat Glands , J Cell Sci 83, 103, 1986
Human Human Keratinocytes Trypsin: 0.25%
 DMEM Cell Cycle Kinetics of Cultured Human Epidermal Keratinocytes , J Invest Dermatol 80, 423, 1983
Human Human, adult epidermal keratinocytes Trypsin: 0.25%
Collagenase: 0.2%
Deoxyribonuclease I: 0.001%
 Eagle's MEM Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells. , J Cell Biol 94, 497-505, 1982
Human Human, fetal Smooth muscle, fibroblasts Trypsin: 0.055%
 Dulbecco's modified Eagle's medium Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts , Biochim Biophys Acta 712, 26, 1982
Human Human, 18-30 years, male, female Fibroblasts Trypsin: 0.1%
 HBSS Effects of Density and Cellular Aging On Collagen Synthesis and Growth Kinetics in Keloid and Normal Skin Fibroblasts , In Vitro 18 (1), 79, 1982
Human Human, newborn Keratinocytes Trypsin: 0.3%
 DMEM Growth Characteristics of Human Epidermal Keratinocytes from Newborn Foreskin in Primary and Serial Cultures , In Vitro 15 (10), 813, 1979
Human Human Keratinocytes Trypsin: 0.25%
 (see reference) Serial Cultivation of Strains of Human Epidermal Kerotinocytes: The Formation of Keratinizing Colonies from Single Cells , Cell 6, 331, 1975
Mouse mouse, male 6-24 week skin side population Collagenase Type 4: 0.2%
Dispase: 1.2 u/ml
 PBS Skeletal muscle engraftment potential of adult mouse skin side population cells. , Proc Natl Acad Sci U S A 100, 9336, 2003
Mouse mouse, 3-7 month fibroblasts, mesangial, smooth muscle Trypsin: 0.25%
Collagenase: see reference
Soybean Trypsin Inhibitor: .05%
 DMEM Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation. , Mol Biol Cell 10, 1569-79, 1999
Porcine porcine, 2-6 month keratinocytes Dispase: 0.25%
 Dulbecco-Vogt MEM Cultured porcine epithelial grafts: an improved method. , J Invest Dermatol 94, 230-4, 1990
Rat Rat, Wistar, 4-8 day Dermal fibroblasts and keratinocytes Trypsin: 0.25%
 Ham's F-12 , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 3A:2.1, 1995
Rat Rat, SD, male, 40-60 days Sebaceous Trypsin: 0.2%
 DMEM with FBS
PBS		 Growth of Sebaceous Cells in Monolayer Culture , In Vitro Cell Dev Biol 28, 83, 1992
Rat Rat, neonatal, 12-24 hr post partum Fibroblasts Trypsin: 0.2%
 HEPES buffered DMEM Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF , Neuron 7, 265, 1991
Rat Rat, albino, one day old, CFN Keratinocytes Trypsin: 1%
 EBSS Growth and Differentiation of Primary Rat Keratinocytes on Synthetic Membranes , In Vitro Cell Dev Biol 22 (3), 141, 1986