Skin - Tissue Tables
| Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
| Frog
|
Frog |
Epidermal |
Trypsin: 0.18%
|
Barth's solution, CMF |
Isolation, characterization, and in vitro culture of larval and adult epidermal cells of the frog Xenopus laevis.
,
In Vitro Cell Dev Biol
26,
1128, 1990 |
| Human
|
human, neonatal |
keratinocytes |
Dispase: 0.4%
Collagenase: 0.3%
|
DMEM |
Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny
,
J Clin Invest 113,
390-400, 2004 |
| Human
|
Human |
mast cells |
Dispase: 0.05%
Collagenase Type 4: 1.0%
|
RPMI |
Comparative cytokine profile of human skin mast cells from two compartments--strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming.
,
J Leukoc Biol Vol. 75,,
,
244-52, 2004 |
| Human
|
Human, adult |
Keratinocytes |
Dispase: 0.25%
|
PBS |
The Tetraspanin CD9 Associates with the Integrin a6B4 in Cultured Human Epidermal Keratinocytes and is Involved in Cell Motility
,
Eur J Cell Biol
79,
41, 2000 |
| Human
|
Human |
keratinocytes |
Dispase: 0.25%
Trypsin: 0.17%
Thermolysin: 0.05%
|
see reference |
Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation.
,
Exp Dermatol Vol. 8,,
,
30-8, 1999 |
| Human
|
Human |
human skin mast cells |
Dispase: 0.1%
Collagenase Type 1: see reference
|
PBS |
Synthesis, storage, and release of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) by human mast cells: implications for the biological significance of VEGF206.
,
Mol Biol Cell
9,
875-84, 1998 |
| Human
|
human |
epidermal |
Collagenase Type 2: 0.1%
Dispase: 0.5-1.0%
|
PBS |
HIV-1 selection by epidermal dendritic cells during transmission across human skin.
,
J Exp Med 187,
1623-31, 1998 |
| Human
|
Human |
Keratinocytes |
Trypsin: 0.05%
|
Dulbecco's PBS |
Culture of Human Keratinocytes in Defined Serum-Free Medium,
Focus 19 (1),
2, 1997 |
| Human
|
Human |
Epidermis plus dermis
Abdomen or inner forearm |
Trypsin: 0.125%
|
DMEM, M199 |
,
Methods in Molecula Biology, Basic Cell Culture Protocols, 2nd ed. Vol. 75,Pollard, J., and Walker, J.,
Humana Press,
23, 1997 |
| Human
|
Human |
Keratinocytes |
Trypsin: 0.25%
|
DMEM |
,
Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons Ltd,
11B:4.1, 1995 |
| Human
|
Human, adult, female, breast skin |
Keratinocytes |
Trypsin: 0.25%
|
PBS |
Expression of Differentiation Markers in Human Adult Keratinocytes Cultured in Submerged Conditions
,
In Vitro Cell Dev Biol
30A,
372, 1994 |
| Human
|
Human, neonatal foreskin |
Fibroblasts |
Trypsin: 0.25%
|
DMEM |
Evaluation of a Biodegradable Matrix Containing Cultured Human Fibroblasts as a Dermal Replacement Beneath Meshed Skin Grafts on Athymic Mice
,
Surgery 111 (1),
438, 1992 |
| Human
|
Human, 18-50 years |
Sweat duct |
Collagenase: 0.03%
|
MEM |
Effects of Media Buffer Systems on Growth and Electrophysiologic Characteristics of Cultured Sweat Duct Cells
,
In Vitro Cell Dev Biol
27,
47, 1991 |
| Human
|
Human |
Fibroblasts |
Trypsin: 0.25%
|
CMF solution |
Postmitotic Human Dermal Fibroblasts Preserve Intact Feeder Properties for Epithelial Cell Growth After Long-Term Cryopreservation
,
In Vitro Cell Dev Biol
26,
709, 1990 |
| Human
|
Human |
Sweat gland |
Collagenase Type 2: 0.015%
|
(see reference) |
Ion Channels in Normal Human and Cystic Fibrosis Sweat Gland Cells
,
Am J Physiol 257,
C129, 1989 |
| Human
|
Human, ages 16-30 |
Human sweat duct |
Collagenase Type 2: 0.2%
|
RPMI 1640
(see reference) |
Human Sweat Duct Cells in Primary Culture. Basic Bioelectric Properties of Cultures Derived From Normals and Patients with Cystic Fibrosis
,
In Vitro Cell Dev Biol
25 (4),
342, 1989 |
| Human
|
Human |
Melanocytes, skin/ foreskin |
Trypsin: 0.25%
|
PBS |
Induction of Nerve Growth Factor Receptors on Cultured Human Melanocytes
,
Proc Natl Acad Sci U S A 85,
5282, 1988 |
| Human
|
Human |
Human sweat glands |
Collagenase: 0.2%
|
HBSS |
The Primary Culture of Epithelia From the Secretory Coil and Collecting Duct of Normal Human and Cystic Fibrotic Eccrine Sweat Glands
,
J Cell Sci
83,
103, 1986 |
| Human
|
Human |
Keratinocytes |
Trypsin: 0.25%
|
DMEM |
Cell Cycle Kinetics of Cultured Human Epidermal Keratinocytes
,
J Invest Dermatol 80,
423, 1983 |
| Human
|
Human, adult |
epidermal keratinocytes |
Trypsin: 0.25%
Collagenase: 0.2%
Deoxyribonuclease I: 0.001%
|
Eagle's MEM |
Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells.
,
J Cell Biol
94,
497-505, 1982 |
| Human
|
Human, fetal |
Smooth muscle, fibroblasts |
Trypsin: 0.055%
|
Dulbecco's modified Eagle's medium |
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts
,
Biochim Biophys Acta
712,
26, 1982 |
| Human
|
Human, 18-30 years, male, female |
Fibroblasts |
Trypsin: 0.1%
|
HBSS |
Effects of Density and Cellular Aging On Collagen Synthesis and Growth Kinetics in Keloid and Normal Skin Fibroblasts
,
In Vitro 18 (1),
79, 1982 |
| Human
|
Human, newborn |
Keratinocytes |
Trypsin: 0.3%
|
DMEM |
Growth Characteristics of Human Epidermal Keratinocytes from Newborn Foreskin in Primary and Serial Cultures
,
In Vitro 15 (10),
813, 1979 |
| Human
|
Human |
Keratinocytes |
Trypsin: 0.25%
|
(see reference) |
Serial Cultivation of Strains of Human Epidermal Kerotinocytes: The Formation of Keratinizing Colonies from Single Cells
,
Cell
6,
331, 1975 |
| Mouse
|
mouse, male 6-24 week |
skin side population |
Collagenase Type 4: 0.2%
Dispase: 1.2 u/ml
|
PBS |
Skeletal muscle engraftment potential of adult mouse skin side population cells.
,
Proc Natl Acad Sci U S A 100,
9336, 2003 |
| Mouse
|
mouse, 3-7 month |
fibroblasts, mesangial, smooth muscle |
Trypsin: 0.25%
Collagenase: see reference
Soybean Trypsin Inhibitor: .05%
|
DMEM |
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation.
,
Mol Biol Cell
10,
1569-79, 1999 |
| Porcine
|
porcine, 2-6 month |
keratinocytes |
Dispase: 0.25%
|
Dulbecco-Vogt MEM |
Cultured porcine epithelial grafts: an improved method.
,
J Invest Dermatol 94,
230-4, 1990 |
| Rat
|
Rat, Wistar, 4-8 day |
Dermal fibroblasts and keratinocytes |
Trypsin: 0.25%
|
Ham's F-12 |
,
Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
3A:2.1, 1995 |
| Rat
|
Rat, SD, male, 40-60 days |
Sebaceous |
Trypsin: 0.2%
|
DMEM with FBS
PBS |
Growth of Sebaceous Cells in Monolayer Culture
,
In Vitro Cell Dev Biol
28,
83, 1992 |
| Rat
|
Rat, neonatal, 12-24 hr post partum |
Fibroblasts |
Trypsin: 0.2%
|
HEPES buffered DMEM |
Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF
,
Neuron 7,
265, 1991 |
| Rat
|
Rat, albino, one day old, CFN |
Keratinocytes |
Trypsin: 1%
|
EBSS |
Growth and Differentiation of Primary Rat Keratinocytes on Synthetic Membranes
,
In Vitro Cell Dev Biol
22 (3),
141, 1986 |
|
|