Reproductive - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine corpus leuteal cells Collagenase Type 4: 420 u/ml
 M-199 Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis. , Endocrinology 142, 5254-60, 2001
Bovine Calf, mid to late gestational Fibroblasts Collagenase: 0.1%
 Medium 199 Characterization of a Fibroblast Cell From the Urinary Bladder Wall , In Vitro Cell Dev Biol 30A, 604, 1994
Bovine Bovine Epithelial Endometrial Collagenase Type 2: 0.1%
 DMEM/EBSS Long-Term Culture of Bovine Trophoblastic Cells, J Tiss Cul Meth 11 (3), 123, 1988
Bovine Bovine
(also porcine, human)		 Interna & corpus luteum Endometrium Ovarian Uterine Pronase: 0.1%
 Moscona's BSS Enzymatic Dissociation of Ovarian and Uterine Tissues , Endocr Res 10, 151, 1984
Frog Xenopus laevis, female oocytes Collagenase Type 1: 0.2%
 see reference Histidines 578 and 587 in the S5-S6 linker of the human Ether-a-gogo Related Gene-1 K+ channels confer sensitivity to reactive oxygen species. , J Biol Chem 277, 8912-9, 2002
Frog Xenopus laevis, female Oocytes Collagenase Type 1: 0.2%
 CF Medium Mechanism of Ba2+ Block of a Mouse Inwardly Rectifying K+ Channel: Differential Contribution by Two Discrete Residues , J Physiol 534 (2), 381, 2001
Frog Xenopus Oocytes Collagenase Type 1: 1%
 (see reference) Identification of XPR-1, A Progesterone Receptor Required for Xenopus Oocyte Activation , Proc Natl Acad Sci U S A 97, 14358, 2000
Frog Xenopus laevis, female Oocytes Collagenase: 0.1%
 Barth's solution, CF Subtype-Specific Effects of Lithium on Glutamate Receptor Function , J Neurophysiol 81, 1506, 1999
Frog Xenopus laevis, mature female Oocytes Collagenase: 0.2%
 CF Medium Beta gamma Subunits of GTP-Binding Proteins Inhibit Muscarinic Receptor Stimulation of Phospholipase C , Proc Natl Acad Sci U S A 85, 8865, 1988
Hamster Hamster, Chinese Ovary Trypsin: 5%
 Dialyzed fetal calf serum, 10% and 0.5M Methotrexate Molecular Determinants of Oligomer Formation and Complement Fixation in Mannose-Binding Proteins , J Biol Chem 274 (6), 3580, 1999
Human human, female, 34-51 yr endometrial epithelial and stromal cells Collagenase Type 3: 0.03%
Deoxyribonuclease I: 0.004%
 DMEM/F-12 Clonogenicity of human endometrial epithelial and stromal cells. , Biol Reprod 70, 1738-50, 2004
Human Human endothelial placental Collagenase Type 1: 0.2%
Trypsin: 0.2%
Deoxyribonuclease I: 0.1%
 DMEM Microvascular endothelial cell activation is present in the umbilical placental microcirculation in fetal placental vascular disease. , Am J Obstet Gynecol Vol. 190,, , 596-601, 2004
Human human amnion epithelial and fibroblast Trypsin: 0.125%
Collagenase: 0.1%
Deoxyribonuclease I: 0.02%
 PBS Enhancement of glucocorticoid-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibroblasts. , Endocrinology 144, 5568-77, 2003
Human Human uterine epithelial cells Pancreatin: 0.34%
Collagenase: 0.16%
Hyaluronidase: 0.16%
 HBSS Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture. , J Infect Dis 185, 1606-13, 2002
Human Human, embryo Endothelial Hematopoietic Stromal Collagenase Type 1/2/4: 0.1%
 DMEM Blood-forming potential of vascular endothelium in the human embryo , Development 129, 4147, 2002
Human Human Stem, embryonic Dispase: 0.01% - 0.02%
 DMEM In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells , Nat Biotechnol 19, 1129, 2001
Human human, 20-40 yr endometrium epithelial and stromal cells Collagenase Type 1: 0.2%
 HBSS Endometrial stromal cells regulate epithelial cell grownth in vitro: a new co-culture model , Hum Reprod 16, 836, 2001
Human human endometrial endothelial cells Collagenase Type 1: 0.2%
 McCoys medium Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium. , Mol Hum Reprod 6, 811, 2000
Human human, female, 20-40 year microvascular endothelial cells Collagenase Type 2: 0.2%
Deoxyribonuclease I: 0.0015%
Trypsin: 0.05%
 PBS Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells. , Hum Reprod 15, 293-301, 2000
Human human, female endometrial epithelial cells Collagenase Type 3: 45 u/ml
Deoxyribonuclease I: .00035%
 DMEM/F-12 Expression of the chemokine eotaxin and its receptor, CCR3, in human endometrium. , Biol Reprod 62, 404-11, 2000
Human human, females, 25-45 yr luteal cells Collagenase Type 2: 0.25%
Deoxyribonuclease I: 0.005%
 PBS Cell characteristics and function of two enriched fraction of human luteal cells prolonged culture. , Mol Hum Reprod 5, 714-9, 1999
Human Human Placental Deoxyribonuclease I: 0.04%
 PSS Expression of the Parathyroid Ca 2+ - Sensing Receptor in Cytotrophoblasts From Human Term Placenta , J Endocrinol 156, 425-430, 1998
Human Human, female endometrial stromal cells Collagenase: 4000 u/ml
 DMEM/F-12 The expression of vascular endothelial growth factor isoforms in cultured human endometrial stromal cells and its regulation by 17beta-oestradiol. , Mol Hum Reprod 4, 603-7, 1998
Human Human Epithelial, fallopian tube Collagenase Type 1: 1%
 Medium 199 Isolation and Monolayer Culture of Human Fallopian Tube Epithelial Cells , In Vitro Cell Dev Biol 27, 720, 1991
Human Human Trophoblasts, placental Trypsin: 0.25%
 EBSS, CMF A Serum-Free System for Culturing Human Placental Trophoblasts , In Vitro Cell Dev Biol 26, 865, 1990
Human Human Trophoblasts, placental Trypsin: 0.25%
 PBS Markers For Human Placental Trophoblasts in Two-Dimensional Gel Electrophoresis , In Vitro Cell Dev Biol 26, 937, 1990
Human Human Chorionic, placental Deoxyribonuclease I: 0.003%
 HBSS Human Placental Chorionic Renin: Production, Purification and Characterization , Biochim Biophys Acta 965, 68, 1988
Human Human, female Endometrial Collagenase: 2%
 RPMI 1640 Gland formation from human endometrial epithelial cells in vitro , In Vitro Cell Dev Biol 24 (10), 1037, 1988
Human Human Placental Trypsin: 0.25%
 DMEM Critical Factors in Establishing Monolayer Cultures of Normal Human Placental Cells in Serum-Free Medium , Endocr Res 12 (3), 229, 1986
Human Human
(also porcine, bovine)		 Interna & corpus luteum Endometrium Ovarian Uterine Pronase: 0.1%
 Moscona's BSS Enzymatic Dissociation of Ovarian and Uterine Tissues , Endocr Res 10, 151, 1984
Human Human, female, 27-49 years Epithelial Ovary Trypsin: 0.125%
 HBSS, CMF Human Ovarian Surface Epithelium In Primary Culture , In Vitro 20, 743, 1984
Human Human Epithelial Stromal Collagenase: 0.25%
 See reference Histochemical Identification of Cultured Cells From Human Endometrium , In Vitro 20 (1), 25, 1984
Human Human, infant and neonate Epithelial Prostate Trypsin: 0.1%
 HBSS Normal Human Prostate Epithelial Cell Cultures , Methods Cell Biol 21, 195, 1980
Human Human, female, 27 years Smooth muscle, uterine Trypsin: 0.05%
 EBSS The Characterization of Human Uterine Smooth Muscle Cells in Culture , Cell Tissue Res 196, 385, 1979
Human Human Epithelial Stromal Collagenase Type 1: 180 u/ml
 DMEM Normal Human Edometrium in Cell Culture , In Vitro 14 (8), 651, 1978
Mouse mouse, 6 day sertoli Collagenase Type 2: 500 u/ml
Hyaluronidase: 0.1%
Deoxyribonuclease I: .0005%
 DMEM Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse. , Biol Reprod 69, 985-94, 2003
Mouse mouse, adult, neonatal and fetal testicular cells Collagenase Type 1: 0.1%
 DMEM/F-12 Adrenocorticotropic hormone directly stimulates testosterone production by the fetal and neonatal mouse testis. , Endocrinology 144, 3279-84, 2003
Mouse Mouse, male, 3.5 months old Leydig Collagenase: 0.06%
 Medium E 199 Effects of Nutrition, Social Factors and Chronic Stress on the Mouse Leydig Cell Testosterone Production, Vet Med 46 (6), 160, 2001
Mouse Mouse, male, 12-14-week-old Seminiferous tubules Trypsin: 0.05%
 DMEM Normal Spermatogenesis in Mice Lacking the Testis-Specific Linker Histone H1t , Mol Cell Biol 20 (6), 2122, 2000
Mouse Mouse, female,6-8 weeks Uterine Trypsin: 0.2%
 HBSS Functional Differentiation of Mouse Uterine Epithelial Cells Grown On Collagen Gels Or Reconstituted Basement Membranes , In Vitro Cell Dev Biol 27, 713, 1991
Mouse Mouse, CF1, female Cumulus, one-cell embryos Hyaluronidase: 0.1%
 PBS, CMF In vitro Development of One-Cell Embryos from Outbred Mice: Influence of Culture Medium Composition , In Vitro Cell Dev Biol 26, 151, 1990
Mouse Mouse (BALB/cCRGL), male, 2-3 months Epithelial, prostate gland Hyaluronidase: 0.1%
 Medium 199 Serum-Free Culture of Enriched Mouse Anterior and Ventral Prostatic Epithelial Cells in Collagen Gel , In Vitro Cell Dev Biol 26, 722, 1990
Mouse Mouse (C57B/T), fetus, 16-17 days old Prostate Trypsin: 1.0%
 HBSS/ DMEM Multistage Carcinogenesis Induced by ras and myc Oncogenes in a Reconstituted Organ , Cell 56, 917, 1989
Mouse Mouse, outbred, CD-1, 21-23 days old Epithelial Trypsin: 0.5%
 Medium 199 Proliferation of Mouse Uterine Epithelial Cells in Vitro , Endocrinology 118 (3), 1011, 1986
Mouse Mouse, BALB/c Epithelial Mesencymal Trypsin: 1%
 DMEM A Simple Efficient Method For Separating Murine Uterine Epithelial and Mesenchymal Cells , Am J Physiol 251, E630, 1986
Mouse Mouse, BALB/cCrg, 40 days old Epithelial Trypsin: 1%
 Medium 199 Restoration of Normal Morphology and Estrogen Responsiveness in Cultured Vaginal and Uterine Epithelia Transplanted with Stroma , Proc Natl Acad Sci U S A 83, 2109, 1986
Mouse Mouse, male, 10-13 wk Leydig Testis Deoxyribonuclease I: 0.001%
 Medium 199 w/ BSA Luteinizing Hormone Receptors and Testosterone Production in Whole Testes and Purified Leydig Cells from the Mouse: Differences among Inbred Strains , Endocrinology 112, 1696, 1983
Mouse Mouse (BALB/cCrgl), female, 50 - 60 days Epithelial, vagina Collagenase Type 3: 0.1%
 HBSS Growth of Normal Mouse Vaginal Epithelial Cells in and on Collagen Gels , Proc Natl Acad Sci U S A 80, 3743, 1983
Porcine porcine, male, 8 day seminiferous epithelial cells Collagenase: 0.15%
Deoxyribonuclease I: .0001%
Hyaluronidase: 0.15%
Trypsin: 0.05%
 DMEM/F12 Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM. , Biol Reprod 61, 225-30, 1999
Porcine Sows, 15 min of slaughter Corpus Leuteum Collagenase Type 4: 600 u/ml
 Medium 199 Assessment of the Mechanism by Which Prolactin Stimulates Progesterone Production by Early Corpora Lutea of Pigs , J Endocrinol 159, 201, 1998
Porcine Pig
(also bovine, human)		 Interna & corpus luteum Endometrium Ovarian Uterine Pronase: 0.1%
 Moscona's BSS Enzymatic Dissociation of Ovarian and Uterine Tissues , Endocr Res 10, 151, 1984
Porcine Piglet 3-4 weeks Leydig Testis Trypsin: 0.0003%
 Lebovitz L-15 Medium Regulaton of Gonadotropin Receptors and Steroidogenesis in Cultured Porcine Leydig Cells , Endocrinology 110, 933, 1982
Rabbit Rabbit, New Zealand white, 4-6 month old, Ovarian Mesothelial Collagenase Type 1: 300 u/ml
 HBSS Growth Stimulation of Ovarian and Extraovarian Mesothelial Cells by Corpus Luteum Extract , In Vitro Cell Dev Biol 29A, 879, 1993
Rabbit Rabbit, New Zealand white, adult, female (nonpregnant),
3-4 kg		 Myocytes, uterine Deoxyribonuclease I: 200 µg/ml
 HBSS-HEPES buffer Alpha-1,Alpha-2,and Beta Adrenergic Signal Transduction in Cultured Uterine Myocytes , In Vitro Cell Dev Biol 26, 369, 1990
Rabbit Rabbit, New Zealand, mature, female, 4-5 Kg Ovarian surface epithelial and peritoneal mesothelial Collagenase Type 1: 300 IU/ml (280 IU/mg)
 HBSS, CMF Isolation and Characterization of Rabbit Ovarian Surface Epithelium, Granulosa Cells, and Peritoneal Mesothelium in Primary Culture , In Vitro Cell Dev Biol 26, 471, 1990
Rabbit Rabbit, mixed breed Endometrial epithelial Collagenase Type 1: 0.005%
 DMEM Changes in Proteins Synthesized by Rabbit Endometrial Epithelial Cells Following Primary Culture , Cell Tissue Res 252, 123, 1988
Rabbit Rabbit, New Zealand white, mature, 3-4 Kg Myometrial Trypsin: 0.02%, 0.03%, 0.0375%
 HBSS Preparation and Characterization of Rabbit Myometrial Cells in Primary Culture: Influence of Estradiol and Progesterone Treatment , In Vitro Cell Dev Biol 23, 93, 1987
Rabbit Rabbit, New Zealand white estrous, female, 4-5 months Mesothelial and surface epithelial Ovaries Trypsin: 0.125%-0.5%
 Medium 199 Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) , Int J Gynecol Pathol 3, 348, 1984
Rat rat, SD, 350-450 g leydig cells Collagenase: .05-0.1%
 Medium 199 Isolation and Culture of Leydig Cells from Adult Rats, Indian J Clinical Biochem 21, 27, 2006
Rat Rat, SD, adult, male, 8 - 10 week Seminiferous tubules Trypsin: 0.05%
 Krebs-Ringer bicarbonate buffer
(see reference)		 Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization , Biol Proced Online 3 (1), 35, 2001
Rat Rat, SD, immature Ovary Collagenase Type 1: 144 u/ml
 McCoy's 5a Non-Steroidal Anti-Inflammatory Drugs (NSAIDS) Block the Late, Prostanoid-Dependent/Ceramide-Independent Component of Ovarian IL-1 Action: Implications for the Ovulatory Process , Mol Cell Endocrinol 157, 21, 1999
Rat Rat, Han-Wistar, adult, male, 250 g Testes Trypsin: 0.1%
 Medium 199 w/ Hank's salts 11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells , J Endocrinol 159, 233, 1998
Rat Rat, SD, female, immature, 26 day
(also rat, pregnant and pseudopregnant)		 Corpus luteum Dispase: 2.4 u/ml Deoxyribonuclease: 200u/ml
 Serum-free medium
(see reference)		 The Expression of Interleukin-6 in the Pregnant Rat Corpus Luteum and Its Regulation by Progesterone and Glucocorticoid , Endocrinology 139 (8), 3597, 1998
Rat Rat, SD, male, 90 days Sertoli Hyaluronidase: 0.1%
 DMEM Pachytene Spermatocyte Protein(s) Stimulate Sertoli Cells Grown in Bicameral Chambers: Dose-Dependent Secretion of Ceruloplasmin, Sulfated Glycoprotein-1, Sulfated Glycoprotein-2, and Transferrin , In Vitro Cell Dev Biol 27, 215, 1991
Rat Rat, SD, male Leydig Trypsin: 0.02%
 DMEM Effect of Cell Purity, Cell Concentration, and Incubation Conditions on Rat Testis Leydig Cell Steroidogenesis , In Vitro Cell Dev Biol 27, 253, 1991
Rat Rat, neonatal Uterine Trypsin: 1%
 HBSS Growth of Neonatal Rat Uterine Luminal Epithelium on Extracellular Matrix , In Vitro Cell Dev Biol 27, 442, 1991
Rat Rat, SD, male, 120-160 g Leydig Adrenal Collagenase Type 2: 0.03% (adrenal)
 Krebs Ringer bicarbonate buffer Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis , In Vitro Cell Dev Biol 26, 24, 1990
Rat Rat, Wistar, adult Uterine Trypsin: 0.5%
 PBS Co-Culture of Two-Cell Rat Embryos on Cell Monolayers , In Vitro Cell Dev Biol 26, 944, 1990
Rat rat, SD, 10 day sertoli cells Collagenase Type 2: 500 u/ml
Deoxyribonuclease I: 0.0005%
Hyaluronidase: 0.1%
 PBS Laminin promotes formation of cord-like structures by Sertoli cells in vitro. , Dev Biol 140, 318-27, 1990
Rat Rat, immature Luteal, ovaries Collagenase: 0.3%
 McCoy's Differential Uptake and Metabolism of Free and Esterified Cholesterol from High-density Lipoproteins in the Ovary , Biochim Biophys Acta 959, 206, 1988
Rat Rat, male, 20 day Sertoli Trypsin: 0.15%
 (see reference) Purification of a Paracrine Factor, P-Mod-S,Produced by Testicular Peritubular Cells That Modulates Sertoli Cell Function , J Biol Chem 263, 2884, 1988
Rat Rat, SD, female Luminal epithelial Trypsin: 0.5%
 HBSS Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells , J Cell Biol 107 (6), 2409, 1988
Rat Rat, Wistar, virgin, female, 250 g Vaginal epithelial Trypsin: 0.5%
 PBS Regulation of Cultured Rat Vaginal Epithelial Cells By 17B-Estradiol and Progesterone , J Steroid Biochem 24 (3), 747, 1986
Rat Rat, SD, female, 21 day Luteal, ovaries Deoxyribonuclease I: 0.0004%
 McCoy's Involvement of Microtubules in Lipoprotein Degradation and Utilization for Steroidogenesis in Cultured Rat Luteal Cells , Endocrinology 117, 2408, 1985
Rat Rat, SD, male, 10 day Sertoli Trypsin: 0.025%
 DMEM Extracellular Matrix Regulates Seratoli Cell Differentiation, Testicular Cord Formation and germ cell development in vitro. , J Cell Biol 101, 1511, 1985
Rat Rat, Holtzman, female, immature Uterine Deoxyribonuclease I: 0.025%
 DMDM buffered with HEPES Estrogen Receptors in Rat Uterine Cell Cultures: Effects of Medium on Receptor Concentration , Endocrinology 115, 762, 1984
Rat Rat, SD, adult,male, 50-70 days Testicular Deoxyribonuclease I: 10 µg/ml
 HEPES Mechanism of the Direct Action of Gonadotropin Releasing Hormone and Its Antagonist on Androgen Biosynthesis by Cultured Rat Testicular Cells , Endocrinology 112, 1653, 1983
Rat Rat, SD, female, pseudopregnant, 21 day Luteal, ovaries Hyaluronidase: 0.1%
 EBSS Binding, Degradation an Utilization of Plasma High Density and Low Density Lipoproteins for Progesterone Production In Cultured Rat Luteal Cells , Endocrinology 112, 1746, 1983
Rat Rat, SD, 4-6 day, male Sertolli, seminiforous tubules Collagenase: 0.03%
 Serum-free medium Age-Dependent Pattern of Androgen-Binding Protein Secretion from Rat Sertoli Cells in Primary Culture , Endocrinology 113, 2284, 1983
Rat Rat, SD, female, 26 days Luteal Deoxyribonuclease I: 0.0004%
 Medium 199 Effect of Antimicrotubule Agents on Microtubules and Steroidogenesis in Luteal Cells , Am J Physiol 243, E380, 1982
Rat Rat, SD, male Leydig Collagenase: 0.1%
 Krebs Ringer bicarbonate buffer The Effects Of Interstitial Cell-Stimulating Hormone, Its Subunits,and Recombinants on Isolated Rat Leydig Cells , Arch Biochem Biophys 167, 294, 1975
Sheep Sheep Epithelial Collagenase: 125 - 190 u/ml
 DMEM The Effects of Estrogen and Progesterone In Vivo on Protein Synthesis and Secretion by Cultured Epithelial Cells from Sheep Endometrium , Endocrinology 117 (5), 2148, 1985