Pituitary - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Calf, male, less then 6 months old Pituitary Collagenase: 0.3%
 DMEM Hassan, H., and Merkel, R.: Perifusion Model System to Culture Bovine Hypothalamic Slices In Series with Dispersed Anterior Pituitary Cells, In Vitro Cell Dev Biol 30A, 435, 1994
Bovine Bovine Pituitary Collagenase: 0.1%
 EBSS, CMF Mason, W. and Ingram, C.: Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells, Vol. 124,, , 207, 1986
Bovine Bovine Follicular, anterior pituitary and pars tuberalis Deoxyribonuclease I: 200 µg/ml
 HBSS, CMF Ferrara, N., Goldsmith, P., Fujii, D., and Weiner, R.: Culture and Characterization of Follicular Cells of the Bovine Anterior Pituitary and Pars Tuberalis, Vol. 124,, , 245, 1986
Bovine Calf, male, 1-6 week old Pitituary Hyaluronidase: 0.1%
 DMEM Ridgway, E., Klibanski, A., Marorana, M., Milbury, P., Kieffer, J., and Chin, W.: The Effect of Somatostatin on the Release of Thyrotropin and its Subunits from Bovine Anterior Pitituary Cells in Vitro, Endocrinology 112 (6), 1937, 1983
Mouse Mouse Pituitary Collagenase Type 2: 0.5%
Deoxyribonuclease I: 0.005%
 HBSS Perez Millain, M., Brinkmeier, M., Mortensen, A. and Camper, S.: PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells., Elife 5, , 2016
Mouse Mouse Pituitary Collagenase Type 1: 1,000 u/ml
Soybean Trypsin Inhibitor: 0.01%
Hyaluronidase: 0.1%
Deoxyribonuclease I: 0.001%
 DMEM Pyczek, J., Buslei, R., Schult, D., Holsken, A., Buchfelder, M., Hess, I., Hahn, H. and Uhmann, A.: Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland., Sci Rep 6, 24928, 2016
Mouse Mouse, male Pituitary Collagenase: 0.4%
Hyaluronidase: 0.1%
Trypsin: 0.3%
 DMEM/Han's F12 Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A: Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase, Endocrinology 144, 188-200, 2003
Ovine Ovine, adult Somatotropes Collagenase Type 1: 0.3%
Hyaluronidase:
Medium 199 Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen: Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes, Endocrinology 143, 4609-19, 2002
Rat Rat, male, 12-15 week Pituitary Collagenase Type 2: 0.4%
Deoxyribonuclease I: 0.04%
 DMEM Akieda-Asai, S., Zaima, N., Ikegami, K., Kahyo, T, Yao, I., Hatanaka, T., Iemura, S., Sugiyama, R., Yokozeki, T., Eishi, Y., Koike, M., Ikeda, K., Chiba, T., Yamaza, H., Shimokawa, I., Song, S., Matsuno, A., Mizutani, A., Sawabe, M. Chao, N., Tanaka, M., : SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5K gamma Activity through Deacetylation of Specific Lysine Residues in Mammals., PLoS ONE 5, e11755, 2010
Rat Rat, Wistar, male, 2 month old Pituitary Trypsin: 0.1%
 DMEM Zhou, X., De Schepper, J., De Craemer, D., Delhase, M., Gys, G., Smitz, J., and Hooghe-Peters, E.: Pituitary Growth Hormone Release and Gene Expression in Cafeteria-Diet-Induced Obese Rats, J Endocrinol 159, 165, 1998
Rat Rat, SD, female, 200 - 250 g Anterior pituitary gland Trypsin: 0.1%
 EBSS, CMF D'Emden, M. and Wark, J.: Culture Requirements for Optimal Expression of 1,25-Dihydroxyvitamin D3-Enhanced Thyrotropin Secretion, In Vitro Cell Dev Biol 27, 197, 1991
Rat Rat Pituitary Trypsin: 0.3 %
 HEPES Wilfinger, W., Larsen, W., Downs, T., and Wilbur, D.: An In Vitro Model for Studies of Intercellular Communication in Cultured Rat Anterior Pituitary Cells, Tissue Cell 16 (4), 483, 1984
Rat Rat, SD, male, 250-450 g Anterior pituitary Trypsin: 0.25%
 Krebs Portanova, R., Smith, D., and Sayers, G.: A Trypsin for the Preparation of Isolated Rat Anterior Pituitary, Proc Soc Exp Biol Med 133, 573, 1970