Pancreas - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Duct epithelial Collagenase: 0.1%
 HEPES Isolation and Culture of Bovine Pancreatic Duct Epithelial cells , Am J Physiol 272, G1328, 1997
Bovine Bovine (Bos taurus) Ductal Neutral Protease: 0.05%
 EBSS Characterization of Bovine Pancreatic Ductal Cells Isolated by a Perfusion-Digestion Technique , In Vitro 19, 651, 1983
Bovine Bovine Platelets Trypsin:
(see reference) Hormone-Sensitive Adneylate Cyclase , J Biol Chem 257 (11), 6287, 1982
Canine Canine Buccal ganglia; SLT muscle Trypsin: 0.2%
 DMEM Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis , Neuron 6, 145, 1991
Fish Fish, Osphronemus gourami, 3-month-old, 0.5 mg Islets Collagenase: 0.12 - 0.46 u/ml
 RPMI 1640 Long-Term Function of Single-Cell Preparations of Piscine Principal Islets in Hollow Fibers , Transplant Proc 24 (6), 2941, 1992
Guinea-Pig Guinea-pig Acinar Collagenase Type 3: 60 u/ml
 Kreb's Ringer Guinea Pig Pancreatic Acini Prepared with Purified collagenase , Exp Cell Res 130, 49, 1980
Guinea-Pig Guinea-pig, Hartley, albino, male, 350-400 g Acinar Soybean Trypsin Inhibitor: 0.01%
 Kreb's Ringer bicarbonate buffer Action of Cholecystokinin and Cholinergic Agents on Calcium Transport in Isolated Pancreatic Acinar Cells , J Clin Invest 56, 366, 1975
Guinea-Pig Guinea-pig Exocrine Hyaluronidase: 0.15% - 0.2%
 Kreb's Ringer Structural and Functional Characterization of Isolated Pancreatic Exocrine Cells , Proc Natl Acad Sci U S A 69 (10), 3028, 1972
Hamster Hamster Islets Collagenase Type 4: 1.3% - 2.0%
 HBSS Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique , Acta Diabetol 12, 208, 1975
Human Human, 25-55 yrs old
(also porcine)		 Islets Collagenase: 0.4%
 Hank's solution The Effect of UW Solution and Its Components on the Collagenase Digestion of Human and Porcine Pancreas , Cell Transplant 4 (6), 615, 1995
Human Human Islets Collagenase Type 4: 0.8%
 HBSS Isolation of Human Pancreatic Islets from Cryopreserved Pancreas, Transplant Proc XVII, 383, 1985
Human Human Islets Collagenase (1 or 4): 0.60%
 HBSS A Method for Isolation of Islets of Langerhans from the Human Pancreas , Diabetes 33, 1055, 1984
Human Human, infant, age 1 day-1 year Islets Collagenase: 170-210 u/ml
 HBSS Infant Human Pancreas: A Potential Source of Islet Tissue for Transplantation , Diabetes 25 (12), 1123, 1976
Monkey Monkey, 3-5 Kg Islets Hyaluronidase: 0.05%
 HBSS Application of an Improved Isolation Technique for Islet Transplantation in Primates and Rats, Transplant Proc 7, 739, 1975
Mouse mouse pancreatic islet Collagenase Type 4: 0.2%
 HBSS The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells. , J Cell Sci 116, 1463-9, 2003
Mouse Mouse, BALB/c, 6-8 week-old, either sex Acinar Collagenase: 0.1%
 Waymouth's MB Analysis and Optimization of Nutritional Set-up for Murine Pancreatic Acinar Cells , JOP 3 (1), 8, 2002
Mouse Mouse Islets Collagenase Type 2: 0.2%
 CF Medium Targeted Overativity of Cell KATP Channels Induces Profound Neonatal Diabetes , Cell 100, 645, 2000
Mouse Mouse Duct Papain: 25 u/ml
 DMEM /F-12 , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:12.1, 1995
Mouse Mouse Acinar Collagenase: 100 u/ml
 HEPES Electrogenic Properties of the Na-Alanine Cotransporter in Acinar Cells , J Membr Biol 94, 99, 1986
Mouse Mouse (C57BL/6J-ob/ob), 9-12 wks, male Islets Hyaluronidase: 0.5%
 Kreb's Ringer bicarbonate buffer Secretion in the Obese (ob/ob) Mouse. The Effect of Oxytetracycline on Insulin Release , Diabetes 32, 932, 1983
Mouse Mouse, male, 18-24 g acinar CLSPA: 70-90 u/ml
Soybean Trypsin Inhibitor: 0.01%
 Krebs-Henseleit Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini. , J Biol Chem 257, 10523-8, 1982
Porcine Pig, 3-months-old, 15-20 kg Acinar Collagenase Type 3: 200 u/ml
 RPMI 1640 Primary Culture of Porcine Pancreatic Acinar Cells , JOP 2 (2), 78, 2001
Porcine Pig, neonatal, 1-3-yr-old, 1.5-2.0 kg, either sex
(also mice, male, 6-8-wk-old)		 Islets Collagenase: 0.25%
 HBSS Large Scale Isolation, Growth, and Function of Porcine Neonatal Islet Cells , J Clin Invest 97 (9), 2119, 1996
Porcine Pig Islets Collagenase: 0.1%-0.2%
 Hank's solution Design of a Simple, in vitro Method for Evaluation of the Efficiency of Crude Clostridium histolyticum Collagenase and its Components for Porcine Islet Isolation, Xenotransplantation 2, 165, 1995
Porcine Piglets, female
(also human)		 Islets Collagenase: 0.1%
 HBSS Islet Isolation from the Pancreas of Large Mammals and Humans: 10 Years of Experience , Exp Clin Endocrinol 103, 3, 1995
Porcine Pig, 0 - 2 years, 200 - 250 kg Islets Collagenase: 0.1%
 HBSS Isolation of Porcine Pancreatic Islets: Low Trypsin Activity During the Isolation Procedure Guarantees Reproducible High Islet Yields , J Clin Lab Anal 8, 407, 1994
Porcine Pig, 10 - 36 months, 200 - 300 kg Islets Collagenase: 0.2%
 HBSS Isolation of the Elusive Pig Islet , Surgery 107 (6), 688, 1990
Porcine Pig Acinar Collagenase: 100 u/ml
 Saline Action of Tetraethylammonium on Calcium-Activated Potassim Channels in Pig Pancreatic Acinar Cells Studied by Patch-Clamp Single-Channel and Whole-Cell Current Recording , J Membr Biol 86, 139, 1985
Rabbit Rabbit, New Zealand white, Male/Female, 2-3 Kg Acinar Hyaluronidase: 0.2%
 Kreb's Ringer bicarbonate buffer Role of Calcium in Exocrine Pancreatic Secretion. IV. Calcium Movements in Isolated Acinar Cells of Rabbit Pancreas , Biochim Biophys Acta 544, 338, 1978
Rabbit Rabbit, also hamster Islets Collagenase Type 4: 1.3% - 2.0%
 HBSS Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique , Acta Diabetol 12, 208, 1975
Rat rat, Wistar, male, 250-400g pancreatic islets Collagenase: 0.75%
 RPMI 1640 Small intestinal submucosa improves islet survival and function during in vitro culture. , World J Gastroenterol 11, 7378, 2005
Rat rat, 250-350 g pancreatic islet Collagenase Type 4: 0.2%
 HBSS The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells. , J Cell Sci 116, 1463-9, 2003
Rat Rat pancreatic acini CLSPA: 30 u/ml
Collagenase Type 4: 30 u/ml
Soybean Trypsin Inhibitor: 0.01%
 M199 Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase. , Am J Physiol Cell Physiol 279, C1993-2003, 2000
Rat Rat, SD, male ancinar CLSPA: see reference
Soybean Trypsin Inhibitor: 0.01%
 DMEM Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180. , J Biol Chem 275, 19115-20, 2000
Rat Rat Islets Collagenase: 126 - 196 u/ml
 HBSS The Role of Protein Kinase C in the Desensitization of Rat Pancreatic Islets to Chrolinergic stimulation , J Endocrinol 159, 287, 1998
Rat Rat Islets Collagenase: 0.5 - 0.9%
 HBSS Culture of Pancreatic Islet Cells, Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., , 12B:11.1, 1995
Rat Rat Duct Papain: 25 u/ml
 DMEM /F-12 , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:12.1, 1995
Rat Rat, SD, male, 40 - 100 g Acinar Hyaluronidase: 462 u/ml
 Ham's F12 Effects of Secretin And Caerulein On Enzymes Of Cultured Pancreatic Acinar Cells , In Vitro Cell Dev Biol 27, 660, 1991
Rat Rat, SD, male, 100 - 150 g Parotid acinar Trypsin: 0.001%
 F12 medium Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells , In Vitro Cell Dev Biol 27, 707, 1991
Rat Rat, Wistar, male Parotid acinar Trypsin: 0.02%
 Solution B
(See reference)		 Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells , J Biol Chem 266, 2778, 1991
Rat Rat, S-Wistar, male, 230 - 270 g
(also mouse, 6 - 8 wk old)		 Islets Collagenase: 0.1% - 0.2%
 HBSS Improvement in the Islet Yield From a Cold-Preserved Pancreas by Pancreatic Ductal Collagenase Distention at the Time of Harvesting , Transplantation 51, 566, 1991
Rat Rat, SD, both sexes, 6-20 wks old Interlobular ducts Papain: 25 u/ml
 DMEM/Ham's F-12 Rat Pancreatic Interlobular Duct Epithelium: Isolation and Culture in Collagen Gel , In Vitro Cell Dev Biol 25 (8), 679, 1989
Rat Rat, SD, male, 150 - 200 g Acinar Soybean Trypsin Inhibitor: 0.01%
 HEPES Cyclic GMP Does Not Inhibit Protein Kinase C-Mediated Enzyme Secretion in Rat Pancreatic Acini , J Biol Chem 264, 995, 1989
Rat Rat, Wistar, male, 250 - 350 g Acinar, parotid Hyaluronidase: 0.015%
 Earle's MEM A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells , Biochem Biophys Res Commun 145, 754, 1987
Rat Rat, SD, male, 50 - 125 g Acinar Exorbital lacrimal, parotid, pancreas Trypsin: 0.01%
 HBSS, CMF Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel , In Vitro Cell Dev Biol 23, 465, 1987
Rat Rat, Fischer-344, either sex, 120-150 g Epithelial Trypsin: 0.1%
 Ham's F-12/HBSS
(see reference)		 Establishment of Propagable Epithelial Cell Lines From Normal Adult Rat Pancreas , Exp Cell Res 168, 365, 1987
Rat Rat, SD, male, 42 - 48 day, 175 - 200 g Acinar, submandibular gland Hyaluronidase: 0.1 %
 HBSS, CF Short-Term Primary Culture of Acinar-Intercalated Duct Complexes From Rat Submandibular Glands , In Vitro Cell Dev Biol 22, 469, 1986
Rat Rat, Dark Agouti and Lewis rat donors, adult Islets Collagenase Type 1: 0.3%
 Hank's solution An Improved Method for the Isolation of Islets of Langerhans From the Adult Rat Pancreas, Transplant Proc XVII (6), 1819, 1986
Rat Rat, SD, male, 50-75 g Acinar Hyaluronidase: 0.1%
 HBSS
(see reference)		 Primary Cultures of Rat Pancreatic Acinar Cells in Serum-Free Medium , In Vitro Cell Dev Biol 21 (1), 6, 1985
Rat Rat, SD, 125 - 350 g Duct Trypsin: 0.01%
 HBSS Ducts of the Rat Pancreas in Agarose Matrix Culture , In Vitro 16, 797, 1980
Rat Rat Exocrine Hyaluronidase: 0.9%
 Kreb's Ringer Isolation and Functional Characterization of Cells From the Exocrine Pancreas, Biology of Normal and Cancerous Exocrine Pancreatic Cells,Ribet, A., Pradayrol, L., and Susini, C., Elsevier, , 1980
Rat Rat, Wistar, male Islets Collagenase Type 4: 1%
 Medium 199 Enhanced Insulin Secretion and Cyclic Amp Accumulation in Pancreatic Islets Due to Activation of Native Calcium Ionophores , J Biol Chem 254 (2), 469, 1979
Rat Rat, SD, male, 250 - 350 g
(also mouse, white, Swiss, male, 20 - 24 g)		 Acinar Hyaluronidase: 0.18%
 Kreb's Henseleit bicarbonate buffer Action of Secretagogues on a New Preparation of Functionally Intact, Isolated Pancreatic Acini , Am J Physiol 235, 517, 1978
Rat Rat, Wistar, male, 200 - 300 g Islets Collagenase Type 4: 0.5%
 HBSS Standardization of a Digestion-Filtration Method for Isolation of Islets , Diabetes 25, 667, 1976
Rat Rat Exocrine Hyaluronidase: 0.15%
 Krebs Calcium Ion Uptake in Isolated Pancreas Cells Induced by Secretagogues , Biochim Biophys Acta 419, 76, 1976
Rat Rat, Wistar-Lewis, fetal 18-20 day gestation
(also neonatal 3-12 day old)		 Islets Collagenase Type 4: 0.63%
 EBSS
(see reference)		 Purification of Monolayer Cell Cultures of the Endocrine Pancreas , Tissue Cell 7 (4), 747, 1975
Rat Rat, neonate Islets Trypsin: 0.05%
 Puck's saline buffered w/ EDTA 0.02% Pancreatic Islet Transplantation in the Rat , Diabetes 22, 413, 1973
Rat Rat, male, albino, 200-300 g Islets Collagenase: 0.5%
 Hanks solution Perifusion of Isolated Rat Islets in Vitro , Diabetes 21 (10), 987, 1972
Rat Rat, Lewis, inbred Islets Collagenase: 0.5%
 HBSS Transplantation of Intact Pancreatic Islets in Rats , Surgery 72 (2), 175, 1972
Rat Rat, Wistar, albino, male, 400 - 500 g Islets Collagenase Type 4: 1.0% - 1.2%
 HBSS Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas , Diabetes 16, 35, 1967
Rat Rat Ascites hepatoma Trypsin: 0.1%
 Phosphate buffer (see reference) Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands , Exp Cell Res 7, 430, 1954