Neural - Tissue Tables
| Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
| Bovine
|
Bovine
(also rat) |
Heart
Adrenal chromaffin
Paraneurons |
Trypsin: 0.06%
|
25mM HEPES buffered Locke's solution, CMF |
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons
,
In Vitro Cell Dev Biol
26,
335, 1990 |
| Bovine
|
Bovine |
Microvascular endothelial |
Collagenase/Dispase: 0.1%
|
MEM |
Primary Culture of Microvascular Endothelial Cells From Bovine Retina
,
In Vitro 18 (7),
626, 1982 |
| Bovine
|
Calf
(also lamb) |
Oligodendroglia
Neural |
Trypsin: 0.1%
|
(see reference) |
Metabolic Properties of Maintained Oligodendroglia Purified from Brain
,
J Biol Chem
253,
1592, 1978 |
| Chicken
|
Chicken, White Leghorn, embryos, 17-21 day |
Neurons |
Papain: 40 u/ml
|
HEPES |
The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus
,
Neuron 9,
173, 1992 |
| Chicken
|
Chick, embryo, 10-14 day |
Flat, retina |
Trypsin: 0.1%
|
Tyrode's solution, CMF |
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina
,
In Vitro Cell Dev Biol
26,
1073, 1990 |
| Chicken
|
Chick, White Leghorn, embryo
(also rat, SD, embryo) |
Spinal cord |
Trypsin: 0.05%
|
Phosphate buffer
(see reference) |
Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions.
,
J Neurosci 1,
204, 1981 |
| Chicken
|
Chick |
Dorsal root ganglion neurons
Spinal cord |
Trypsin: 0.1%
|
Puck's saline, CMF |
GABA Conductance of Chick Spinal Cord and Dorsal Root Ganglion Neurons in Cell Culture
,
J Neurophysiol 45,
605, 1981 |
| Chicken
|
Chick, White Leghorn embryo, 8 day |
Neurons, ganglia |
Trypsin: 0.25%
|
HBSS, CMF |
Selective Survival of Neurons from Chick Embryo Sensory Ganglionic Dissociates Utilizing Serum-Free Supplemented Medium
,
Exp Cell Res
125,
183, 1980 |
| Chicken
|
Chicken, White Leghorn, fertile eggs |
Ciliary ganglion neurons |
Trypsin: 0.25%
|
Eagle's MEM |
Long-Term Survival and Development of Dissociated Parasympathetic Neurons in Culture
,
Brain Res 183,
161, 1980 |
| Chicken
|
Chicken, embryos, 9-10 days old |
Dorsal root ganglia neurons |
Collagenase: 0.01%
|
Eagle's MEM |
Enkephalin Inhibits Release of Substance P From Sensory Neurons in Culture and Decreases Action Potential Duration
,
Proc Natl Acad Sci U S A 76 (1),
526, 1979 |
| Chicken
|
Chick embryos |
Ganglion chains, sympathetic ganglia |
Trypsin: 0.25%
|
Krebs Phosphosaline |
Preparation of Pure Neuronal and Non-Neuronal Cultures From Embryonic Chick Sympathetic Ganglia: A New Method Based on Both Differential Cell Adhesiveness and the Formation of Homotypic Neuronal Aggregates
,
Brain Res 114,
391, 1976 |
| Fish
|
Black ghose knige fish, adult (Apteronotus albifrons) |
Neurons, spinal cord |
Trypsin: 0.4%
|
PBS, CMF |
Differences in Growth of Neurons from Normal and Regenerated Teleost Spinal Cord in vitro
,
In Vitro Cell Dev Biol
29A,
145, 1993 |
| Frog
|
Xenopus, embryonic |
Neuron |
Collagenase Type 1: 0.1%
|
Steinberg's solution |
Structure and Physiology of Developing Neuromuscular Synapses in Culture
,
J Neurosci 7,
473, 1987 |
| Guinea-Pig
|
Guinea-pig, newborn |
Neuron, enteric |
Trypsin: 0.125%
|
Medium 199 |
Tissue Culture of Mammalian Enteric Neurons
,
Brain Res 152,
573, 1978 |
| Hamster
|
hamster, male |
vomeronasal organ neurons |
Collagenase Type 1: 0.02%
Trypsin: 0.02%
|
PBS |
Regulation by voltage and adenine nucleotides of a Ca2+-activated cation channel from hamster vomeronasal sensory neurons.
,
J Physiol
548,
777, 2003 |
| Human
|
human, 9 month |
neurons |
Collagenase Type 4: 1.33%
Papain: 0.07 u/ml
Neutral Protease: 1 mg/ml
|
DMEM/F12 |
EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy
,
Nat Med 11,
277, 2005 |
| Human
|
human, 26 week |
neural progenitor cells |
Papain: 2.5 u/ml
Deoxyribonuclease I: 250 u/ml
Neutral Protease: 1 u/ml
|
DMEM/F-12 |
Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells.
,
J Neurosci Res 75,
782, 2004 |
| Human
|
human, adult |
ventricular epithelial |
Papain: 11.4 u/ml
Deoxyribonuclease I: 10 u/ml
|
DMEM/F12 |
Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone.
,
J Neurosci Res 59,
321, 2000 |
| Human
|
Human, 5-65 years |
Retinal pigment epithelial (RPE) |
Trypsin: 0.25%
|
HBSS |
Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture
,
Exp Eye Res 69,
97, 1999 |
| Human
|
human |
dorsal root ganglion neurons |
Collagenase Type 1: 0.2%
Dispase: 0.5%
|
DMEM/F-12 |
Two tetrodotoxin-resistant sodium channels in human dorsal root ganglion neurons.
,
FEBS Lett
462,
117, 1999 |
| Mouse
|
mouse, 6 mo |
neurons, neurospheres |
Papain: 0.2%
|
Hibernate |
Isolation and culture of adult neurons and neurospheres.
,
Nat Protoc Vol. 2,,
,
1490-8, 2007 |
| Mouse
|
Mouse, C57BL/6, 1 year |
Neurons |
Papain: 0.2%
|
DMEM |
Culture of Adult Mouse Neurons
,
Biotechniques
38(1),
99-104, 2005 |
| Mouse
|
mouse, adult |
brain and spinal cord cells |
Trypsin: 0.25%
|
PBS |
Expression of a dominant negative IFN-gammareceptor on mouse oligodendrocytes.
,
Glia 51,
22-34, 2005 |
| Mouse
|
mouse, neonatal |
neurons |
PDS kit:
|
EBSS |
Developmental regulation of gonadotropin-releasing hormone neurons by fibroblast growth factor signaling.
,
Endocrinology 145,
3830, 2004 |
| Mouse
|
mouse, C57BL |
cerebellar granule cell precursors |
Papain: 16.5 u/ml
Deoxyribonuclease I: 0.008%
|
Dulbecco's PBS |
Cerebellar granule cell precursors can differentiate into astroglial cells
,
Proc Natl Acad Sci U S A 101,
1211, 2004 |
| Mouse
|
mouse, 1-3 day |
neurons, ganglia |
Papain: 20 u/ml
Deoxyribonuclease I: 100 u/ml
Collagenase: 0.3%
Trypsin: 0.05%
|
HBSS |
Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody.
,
Mol Cell Neurosci 24,
1131, 2003 |
| Mouse
|
Mouse, postnatal-day-1-old (P1) |
Neurons |
Trypsin: 0.25%
|
NGF-containing medium |
Caspase Inhibition Extends the Commitment to Neuronal Death Beyond Cytochrome c Release to the Point of Mitochondrial Depolarization
,
J Cell Biol
150 (1),
131, 2000 |
| Mouse
|
Mouse embryos |
Neurons, DRG and SCG |
Trypsin: 0.25%
|
L-15 medium |
P75-Eficient Embryonic Dorsal Root Sensory and Neonatal Sympathetic Neurons Display a Decreased Sensitivity to NGF
,
Development
120,
1027, 1994 |
| Mouse
|
65 days |
neurons, neuronal precursors |
Trypsin: 0.1%
Deoxyribonuclease I: 0.001%
|
DMEM |
De Novo Generation of Neuronal Cells from the Adult Mouse Brain
,
Proc Natl Acad Sci U S A 89,
8591, 1992 |
| Mouse
|
Mouse, CD-1, neonate |
Neurons, dorsal root ganglion |
Trypsin: 0.25%
|
HBSS |
Enhanced Neuronal Regeneration by Retinoic Acid of Murine Dorsal Root Ganglia and of Fetal Murine and human Spinal Cord in vitro
,
In Vitro Cell Dev Biol
27,
55, 1991 |
| Mouse
|
Mouse, fetal |
Precursor |
Trypsin: 0.5%
|
PBS |
Isolation and Characterization Of Mouse Neural Precursor Cells in Primary Culture
,
In Vitro Cell Dev Biol
27,
615, 1991 |
| Mouse
|
Mouse (SWR or CF1), 1-3 months |
Papillae, taste receptor |
Pronase E: 0.15%
|
Carbonate-Phosphate buffer
(see reference) |
A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells
,
Brain Res 503,
326, 1989 |
| Mouse
|
Mouse, neonatal
(also chick) |
PNS test neurons |
Trypsin: 0.08%
|
Eagle's Basal Medium (see reference) |
Low Molecular Weight Agents Support Survival of Cultured Neurons From the Central Nervous System
,
J Neurosci 4 (3),
654, 1984 |
| Mouse
|
Mouse (BALB/c), adult |
Neurons, spinal cord |
Collagenase Type 3: 0.25%
|
Hank's BSS, CMF |
Survival of Purified Motor Neurons In Vitro: Effects of Skeletal Muscle-Conditioned Medium
,
Neurosci Lett 38,
187, 1983 |
| Mouse
|
Mouse, 0-30 day |
Neural |
Trypsin NF 1:250: 50 0.25%
|
BSS |
Cultured Cell Systems and Methods for Neurobiology
,
Methods Enzymol 32,
765, 1974 |
| Porcine
|
porcine, adult, 60-100 kg |
superior cervical ganglia |
Papain: 2 u/ml
Collagenase: 0.12%
Dispase: 0.48%
|
HBSS |
Presynaptic alpha7-nicotinic acetylcholine receptors mediate nicotine-induced nitric oxidergic neurogenic vasodilation in porcine basilar arteries.
,
J Pharmacol Exp Ther 298,
122, 2001 |
| Quail
|
Quail |
Neural crest |
Trypsin: 0.05%
|
MEM, HBSS |
Clonal Analysis of Quail Neural Crest Cells: They are Pluripotent and Differentiate in Vitro in the Absence of Noncrest Cells
,
Dev Biol 80,
96, 1980 |
| Rat
|
Rat, Wistar, 4 day |
hippocampal neurons |
PDS kit:
|
Neurobasal A |
Cross-talk of vitamin D and glucocorticoids in hippocampal cells.
,
J Neurochem 96,
500-9, 2006 |
| Rat
|
rat, 2 month |
spinal cord progenitor cells
Hybrid toxins |
PDS kit: see reference
|
Neurobasal A |
Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells
,
J Histochem Cytochem
53(10),
1215, 2005 |
| Rat
|
rat, SD, E19 |
dorsal root ganglia |
PDS kit:
|
MEM/Ham's F12 |
AMG 9810 [(E)-3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo[b][1,4] dioxin-6-yl)acrylamide], a novel vanilloid receptor 1 (TRPV1) antagonist with antihyperalgesic properties.
,
J Pharmacol Exp Ther 313,
474, 2005 |
| Rat
|
rat, Wistar, E15 |
vomeronasal receptor neurons |
Collagenase/Dispase: 0.1%
Papain: 0.5 u/ml
|
DMEM/F12 |
Maturation of vomeronasal receptor neurons in vitro by coculture with accessory olfactory bulb neurons.
,
Chem Senses
30,
111, 2005 |
| Rat
|
rat, SD, 1-2 day |
cortical astrocytes |
Papain: see reference
|
DMEM |
Mechanical strain injury increases intracellular sodium and reverses Na+/Ca2+ exchange in cortical astrocytes.
,
Glia 51,
35-46, 2005 |
| Rat
|
rat, fetal |
brainstem and cortical neurons |
PDS kit:
|
DMEM |
Extrahypothalamic expression of the glucagon-like peptide-2 receptor is coupled to reduction of glutamate-induced cell death in cultured hippocampal cells.
,
Endocrinology 145,
3495, 2004 |
| Rat
|
rat, SD, male, 270-330 g |
spinal progenitor cells |
PDS kit: with modifications
|
Neurobasal medium |
Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain
,
Cell Transplant 11,
17, 2002 |
| Rat
|
rat, Fisher, 8-9 week |
adult progenitor |
Papain: 2.5 u/ml
Deoxyribonuclease I: 250 u/ml
Dispase: 1 u/ml
|
DMEM/F-12 |
The adult substantia nigra contains progenitor cells with neurogenic potential
,
J Neurosci 22,
6639, 2002 |
| Rat
|
rat. 15 week |
neurons |
Papain: 0.2%
|
Hibernate A |
Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem.
,
J Neurophysiol 87,
1076, 2002 |
| Rat
|
Rat |
Neurons, hippocampal |
Papain: 15 - 20 u/ml
|
Eagle's MEM
(see reference) |
B-Amyloid Peptide Blocks the Response of a7-Containing Nicotinic Receptors on Hippocampal Neurons
,
PNAS
98 (8),
4734, 2001 |
| Rat
|
Rat, newborn, 7 days old |
Neurons |
Trypsin: 0.25%
|
MEM10 |
A Sensory Neuron Subpopulation with Unique Sequential Survival Dependence on Nerve Growth Factor and Basic Fibroblast Growth Factor during Development
,
J Neurosci 21 (22),
8873, 2001 |
| Rat
|
Rat, embryonic 18-19 day |
hippocampal neurons |
PDS kit: see reference
|
DMEM |
Phosphorylation of cAMP response element-binding protein in hippocampal neurons as a protective response after exposure to glutamate in vitro and ischemia in vivo.
,
J Neurosci 21,
9204-13, 2001 |
| Rat
|
Rat, embryonic, day 18 |
Cortical |
Papain:
|
Neurobasal medium and DMEM |
Immobilization of Neural Cells in Three-Dimensional Matrices for Biosensor Applications
,
Biosensors & Bioelectronics 14,
871, 2000 |
| Rat
|
Rat, Wister, postnatal, P1-3 days |
Neurons, hippocampal |
Papain: 20 u/ml
|
EBSS |
Activity-Dependent Formation of Perforated Synapses in Cultured Hippocampal Neurons
,
Eur J Neurosci 11,
4241, 1999 |
| Rat
|
Rat, SD, female, pregnant |
Sciatic nerves |
Trypsin: 0.025%
|
L-15 medium
(see reference) |
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells
,
Cell
96,
737, 1999 |
| Rat
|
Rat
(also mice) |
Spinal cord |
Trypsin: 0.133%
|
HBSS and PIPES |
Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System
,
Cell
96,
25, 1999 |
| Rat
|
Rat, (Long Evans), 2-5 day old |
Neurons, hippocampal |
Papain: 20 u/ml
|
EBSS |
Activation and Desensitization of Hippocampal Kainate Receptors
,
J Neurosci 17 (8),
2713, 1997 |
| Rat
|
Rat, Wister and SD, newborn, 0-21 days, either sex |
Myenteric ganglia |
Trypsin: 0.05%
|
MEM-HEPES |
A New Method for the Isolation of Myenteric Plexus from the Newborn Rat Gastrointestinal Tract
,
Brain Res Proto 1,
109, 1997 |
| Rat
|
Rat, pups, 24-48 h old |
Neurons, hippocampal |
Papain: 20 u/ml
|
Harvest buffer |
Differential Surface Expression and Phosphorylation of the N-Methyl-D-Aspartate Receptor NR1 and NR2 in Cultured Hippocampal Neurons
,
J Biol Chem
272 (7),
4135, 1997 |
| Rat
|
Rat, SD, adult, 250-300g, P8 |
Neurons, DRG |
Dispase: 0.5%
|
L-15 w/ CO2 |
Regeneration of Adult Axons in White Matter Tracts of the Central Nervous System
,
Nature
390,
680, 1997 |
| Rat
|
Rat, 1-4 day old |
Neurons, hippocampal |
Papain: 20 u/ml
|
MEM |
Cannabinoids Inhibit N- and P/Q-Type Calcium Channels in Cultured Rat Hippocampal Neurons
,
J Neurophysiol 78,
43, 1997 |
| Rat
|
SD, Fisher |
hippocampal neurons |
Papain: 0.2%
|
HibernateA/B27 |
Isolation and culture of adult rat hippocampal neurons of any age.
,
J Neurosci Methods 71,
143, 1997 |
| Rat
|
rat, E18 |
hippocampal neurons |
Papain: 20 u/ml
Deoxyribonuclease I: 0.01%
|
MEM |
Upregulation of GABAA current by astrocytes in cultured embryonic rat hippocampal neurons.
,
J Neurosci 16,
2912-23, 1996 |
| Rat
|
Rat, pups, 14 day old |
Neurons, sympathetic |
Dispase: 0.24%
|
HBSS |
Extrinsic Factors Influence the Expression of Voltage-Gated K Currents on Neonatal Rat Sympathetic Neurons
,
J Neurosci 13 (6),
2591, 1993 |
| Rat
|
Rats, SD, 11-14 day old, 29-32 g |
Basal forebrain neurons |
Trypsin: 0.125%
|
Gey's BSS |
The Whole-Cell Calcium Current in Acutely Dissociated Magnocellular Cholinergic Basal Forebrain Neurones of the Rat
,
J Physiol
460,
91, 1993 |
| Rat
|
Rat, Wistar-Hanover, 7-12 day |
Postnatal dopamine neurons |
Trypsin: 0.035%
|
(see reference) |
Identified Postnatal Mesolimbic Dopamine Neurons in Culture: Morphology and Electrophysiology
,
J Neurosci 12 (11),
4264, 1992 |
| Rat
|
Rat, SD, 18-day-old, fetus |
Hippocampal |
Trypsin: 0.2%
|
HBSS |
IGF-I and IGF-II Protect Cultured Hippocampal and Septal Neurons Against Calcium-Mediated Hypoglycemic Damage
,
J Neurosci 12 (4),
1558, 1992 |
| Rat
|
Rat |
Stem, neural crest |
Collagenase: 0.075%
|
Ringer's solution |
Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest
,
Cell
71,
973, 1992 |
| Rat
|
Rat, SD, pregnant, Charles River |
Pyramidal neurons
Nonpyramidal neurons |
Trypsin: 0.027%
|
HEPES |
Electrophysiological Comparison of Pyramidal and Stellate Nonpyramidal Neurons in Dissociated Cell Culture of Rat Hippocampus
,
Brain Res Bull 26,
333, 1991 |
| Rat
|
Rat, SD, embryos, 19-21 days gestation |
Schwann, dorsal root ganglia |
Trypsin: 0.25%
|
HBSS, CMF |
Effects of Ethanol on Rat Schwann Cell Proliferation and Myelination in Culture
,
In Vitro Cell Dev Biol
26,
129, 1990 |
| Rat
|
Rat
(also bovine) |
Heart
Adrenal chromaffin
Paraneurons |
Trypsin: 0.06%
|
25mM HEPES buffered Locke's solution, CMF |
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons
,
In Vitro Cell Dev Biol
26,
335, 1990 |
| Rat
|
Rat, SD, 8 day old pups |
Cerebellar neurons |
Trypsin: 0.025%
|
Eagle's MEM |
Glutamate Becomes Neurotoxic Via the N-methyl-D-aspartate Receptor When Intracellular Energy Levels are Reduced
,
Brain Res 451,
205, 1988 |
| Rat
|
Rat, fetuses, 18 day old |
Hippocampal neurons |
Trypsin: 0.2%
|
Eagle's MEM |
Isolated Hippocampal Neurons in Cryopreserved Long-Term Cultures: Development of Neuroarchitecture and Sensitivity to Nmda
,
Int J Dev Neurosci 6 (5),
439, 1988 |
| Rat
|
Rat, postnatal |
Septal neurons |
Papain: 0.05%
|
PBS, CMF |
Septal Cholinergic Neurons From Postnatal Rat Can Survive In The Dissociate Culture Conditions In The Presence Of Nerve Growth Factor
,
Neurosci Lett 79,
85, 1987 |
| Rat
|
Rat, Long Evans, 1-15 days |
Neurons, visual cortex |
Papain: 20 u/ml
|
BSS see reference
(see reference) |
Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats
,
J Neurosci 6,
3044, 1986 |
| Rat
|
Rat, SD, female, 4 month old |
CNS cells |
Trypsin: 0.25%
|
EBSS |
Evidence That Axons are Mitogenic for Oligodendrocytes Isolated From Adult Animals
,
Nature
320,
756, 1986 |
| Rat
|
Rat, embryo, 15 day |
Dorsal horn neurons
Spinal |
Trypsin: 0.025%
|
Ham's F-12 |
Synaptic Transmission between Dorsal Root Ganglion and Dorsal Horn Neurons in Culture: Antagonism of Monosynaptic Excitatory Postsynaptic Potentials and Glutamate Excitation by Kynurenate
,
J Neurosci 5,
2281, 1985 |
| Rat
|
Rat, fetus, 18-20 day |
Hippocampal neurons |
Trypsin: 0.25%
|
HBSS, CMF |
An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts
,
J Neurosci 4,
1944, 1984 |
| Rat
|
Rat, postnatal |
Ganglion, retina |
Papain: 12.5 u/ml
|
HBSS w/5 mM HEPES |
Monoclonal Antibody to Thy-1 Enhances Regeneration of Processes by Rat Retinal Ganglion Cells in Culture
,
Science 224,
303, 1984 |
| Rat
|
Rat, SD, neonatal |
Retinal ganglion |
Trypsin: 0.25%
|
CMF medium /Ham's F-10 with FCS |
Retrograde Labeling, Enrichment, and Characterization of Retinal Ganglion Cells from the Neonatal Rat
,
J Neurosci 3,
2532, 1983 |
| Rat
|
Rat, SD, pups |
Retina |
Trypsin: 0.25%
|
Ham's F-12 |
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat
,
J Neurosci 3 (12),
2532, 1983 |
| Rat
|
Rat, W/FU, 5-8 day |
Neurons and glial |
Trypsin: 0.25%
|
MEM
(see reference) |
Cell-Type-Specific Markers for Distinguishing and Studying Neurons And the Major Classes of Glial Cells in Culture
,
Brain Res 174,
283, 1979 |
| Rat
|
Rat, fetus |
Neurons, sympathetic |
Trypsin: 0.25%
|
L-15 or HBSS, CMF |
Postnatal Rat Sympathetic Neurons In Culture. 1. A Comparison With Embryonic Neurons
,
J Neurophysiol 42,
1410, 1979 |
| Rat
|
Rat, Wistar/Furth, newborn
(also bovine) |
Schwann |
Trypsin: 0.25%
|
DMEM |
Studies on Cultured Rat Schwann Cells. I. Establishment of Purified Populations From Cultures of Peripheral Nerve
,
Brain Res 165,
105, 1979 |
| Rat
|
Rat, embryo |
Neurons, cortical |
Trypsin: 0.027%
|
MEM |
Rat Cortical Neurons in Cell Culture: Culture Methods, Cell Morphology, Electrophysiology, and Synapse Formation
,
Brain Res 149,
279, 1978 |
| Rat
|
Rat, newborn |
Neurons, sympathetic |
Collagenase Type 1: 0.01%
|
Hank's solution, CF |
Neurotransmitter Synthesis and Uptake by Isolated Sympathetic Neurones in Microcultures
,
Nature
270,
147, 1977 |
| Rat
|
Rat, neonatal |
Neurons, superior cervical ganglia |
Trypsin: 0.1%
|
Basal L-15 medium |
Primary Cultures of Dissociated Sympathetic Neurons I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties
,
J Cell Biol
59,
329, 1973 |
| Salamander
|
Salamander, 18-25 cm |
Retina |
Papain: 14 u/ml
|
Saline |
Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase
,
J Cell Biol
100,
175, 1985 |
| Salamander
|
Salamander (A.tigrinum) |
Photoreceptors, retina |
Papain: 0.05%
|
(see reference) |
Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina
,
Proc Natl Acad Sci U S A 75,
3507, 1978 |
| Sheep
|
Lamb
(also calf) |
Oligodendroglia
Neural |
Trypsin: 0.1%
|
(see reference) |
Metabolic Properties of Maintained Oligodendroglia Purified from Brain
,
J Biol Chem
253,
1592, 1978 |
| Shellfish
|
Snails (Helisoma trivolis), albino, adult |
Buccal ganglia; SLT muscle |
Trypsin: 0.2%
|
DMEM |
Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis
,
Neuron 6,
145, 1991 |
| Shellfish
|
Pond snail (Helisoma) albino, adult |
Somata, buccal ganglia |
Trypsin: 0.2%
|
Antibiotic saline, Leibowitz 50% |
The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata
,
J Neurosci 8,
1032, 1988 |
| Shellfish
|
Helisoma trivovlis |
Buccal ganglia |
Trypsin: 0.2%
|
Liebowitz L-15 medium |
Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma
,
J Neurosci Res 13,
285, 1985 |
| Shellfish
|
Mollusc, juvenile 1-2 gm or adult 50-100 gm |
Neurons
LUQ cells |
Protease: 1%
|
L15 medium |
Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment
,
J Neurosci 3 (12),
2403, 1983 |
| Shellfish
|
Aplysia californica |
LUQ cells
RUQ cells |
Protease: 1%
|
L15 medium |
Identified Aplysia Neurons Form Specific Chemical Synapses in Culture
,
J Neurosci 3 (12),
2614, 1983 |
| Turtle
|
Turtle (Pseudemys scripta elegans) |
Retinal |
Papain: 0.1% (13.5 u/mg)
|
Kreb's Ringer |
Biosynthesis of Acetylcholine in Turtle Photoreceptors
,
Proc Natl Acad Sci U S A 69,
1987, 1972 |
|
|