Muscle - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Smooth muscle Trypsin: 0.25%
 DMEM Absher, M., Woodcock-Mitchell, J., Mitchell, J., Baldor, L., Low, R., and Warshaw, D.: Characterization of Vascular Smooth Muscle Cell Phenotype in Long-Term Culture, In Vitro Cell. Dev. Biol. 25 (2), 183, 1989
Bovine Bovine Vascular smooth muscle Elastase Type 3: 50 u/ml
 PSS Warshaw, D., Szarek, J., Hubbard, M., and Evans, J.: Pharmacology and Force Development of Single Freshly Isolated Bovine Cartoid Artery Smooth Muscle Cells, Circ. Res. 58, 399, 1986
Bovine Bovine Smooth muscle, fibroblasts Trypsin: 0.055%
 DMEM Davies, P. and Kerr, C.: Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts, Biochim. Biophys. Acta 712, 26, 1982
Canine Dog Smooth muscle Elastase: 50 u/ml
 PSS Subramanian, M., Madden, J., and Harder, D.: A Method for the Isolation of Cells from Arteries of Various Sizes, J. Tiss. Cult. Meth. 13, 13, 1991
Canine Dog, beagle, adult Smooth muscle
Vascular		 Elastase: 34 u/ml
 Tyrode's solution w/ calcium Wilde, D., and Lee, K.: Outward Potassium Currents in Freshly Isolated Smooth Muscle Cell of Dog Coronary Arteries, Circ. Res. 65, 1718, 1989
Canine Dog Artery
Carotid		 Elastase: 80 u/ml
 PSS Dobrin, P., and Canfield, T.: Elastase, Collagenase, and the Biaxial Elastic Properties of Dog Carotid Artery, Am. J. Physiol. Heart Circ. Physiol. 247 (16), H124, 1984
Chicken Chicken, 1-2 day Gizzard and aorta smooth muscle Collagenase Type 1: 0.15%
 HBSS Dirksen W., Vladic F., and Fisher S.: A Myosin Phosphatase Targeting Subunit Isoform Transition Defines a Smooth Muscle Developmental Phenotypic Switch, Am. J. Physiol. / Cell 278(3), C589, 2000
Chicken Chick Smooth muscle Trypsin: 0.05% - 0.1%
 HBSS Chamley-Campbell, J., Campbell, G., and Ross, R.: The Smooth Muscle in Cell Culture, Physiol. Res. 59, 1, 1979
Chicken Chick, white leghorn, 12 day Muscle Trypsin: 0.25%
 Puck's saline A Bullaro, J., and Brookman, D.: Comparison of Skeletal Muscle Monolayer Cultures Initiated With Cells Dissociated by the Vortex and Trypsin Methods, In Vitro 12, 564, 1976
Chicken Chick, white leghorn, embryos, 11 day Muscle Trypsin: 0.05%
 Saline G Tepperman, K., Morris, G., Essien, F., and Heywood, S.M.: A Mechanical Dissociation Method For Preparation of Muscle Cell Cultures, J. Cell. Physiol. 86, 561, 1975
Chicken Chick embryo Thyroid
Muscle
Heart		 Collagenase: 0.25%
 Tyrode's saline, potassium free Hilfer, S., and Brown, J.: Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart, Exp. Cell Res. 65, 246, 1971
Chicken Chick, embryonic Muscle Trypsin: 0.1%
 CMF HBSS Hilfer, S.: Collagenase Treatment of Chick Heart and Thyroid,Tissue Cult Methods & Applications,Kruse, P., and Patterson, M., , 246, 1971
Chicken Chick embryonic Various tissues (heart, liver, skeletal, cardiac) Trypsin: various grades
 CMF Tyrode's solution Rinaldini, L.: An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells, Exp. Cell Res. 16, 477, 1959
Feline Cat, adult mongrel, either sex, 2.5-4.0 kg Cerebral arteries Elastase: 50 u/ml
 Puck's solution Madden, J., Vadula, M., and Kurup, V.: Effects of Hypoxia and Other Vasoactive Agents on Pulmonary and Cerebral Artery Smooth Muscle Cells, Am. J. Physiol. 263, L384, 1992
Feline Cat, mongrel, adult, 2-4 kg Myocytes Collagenase: 0.12%
 Kreb's Henseleit, CF Follmer, C.H., Ten Eick, R.E., and Yeh, J.Z.: Sodium Current Kinetics in Cat Atrial Myocytes, J. Physiol. 384, 169, 1987
Fish Dogfish, Squalus acanthias , 2-6 Kg
(also guinea-pig, rabbit, frog)		 Myocytes, heart and stomach Protease XIV: 0.028%
 Solution C
(see reference)		 Mitra, R. and Morad, M.: A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates, Am. J. Physiol. 249, H1056, 1985
Frog Frog, Xenopus laevis, embryos stage 17 & 19 Muscle Collagenase: 0.10%
 Steinberg's solution Stollberg, J. and Fraser, S.: Acetylcholine Receptors and Con A-Binding Sites on Xenopus Muscle Cells, J. Cell Biol. 107, 1397, 1988
Frog Frog Myocytes Trypsin: 0.1%
 CF Ringer Shepherd, N. and Kavaler, F.: Direct Control of Contraction Force of Single Frog Atrial Cells by Extracellular Ions, Am. J. Physiol. 251, C653, 1986
Frog Frog, Rana pipiens (50-100 g)
(also guinea-pig, rabbit, dogfish, Squalus acanthias )		 Myocytes, heart and stomach Protease XIV: 0.028%
 Solution C
(see reference)		 Mitra, R. and Morad, M.: A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates, Am. J. Physiol. 249, H1056, 1985
Frog Frog, Xenopus laevis Muscle Trypsin: 0.5%
 L15 medium
(see reference)		 Anderson, M.J., Cohen, M.W., and Zorychta, E.: Effects of Innervation on the Distribution of Acetylcholine Receptors on Cultured Muscle Cells, J. Physiol. 268, 731, 1977
Guinea-Pig Guinea-pig Bladder smooth muscle Collagenase Type 2: 0.1-0.2%
 Krebs-Ringer bicarbonate Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M: Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells, J. Pharmacol. Exp. Ther. 296, 669-75, 2001
Guinea-Pig Guinea-pig, 200-380 g Capillaries
Myocytes		 Collagenase Type 2: 0.15%
 CF solution Schnitzler, M., Derst, C., Daut, J., and Preisig-Muller, R.: ATP-Sensitive Potassium Channels in Capillaries Isolated From Guinea-Pig Heart, J. Physiol. 525 (2), 307, 2000
Guinea-Pig Guinea pig, 2-4 wk old, male, female Smooth muscle
Gallbladder		 Papain: 0.1%
 Krebs solution Firth, T., Mawe, G., and Nelson, M.: Pharmacology and Modulation of KATP Channels by Protein Kinase C and Phosphatates in Gallbladder Smooth Muscle, Am. J. Physiol. Cell Physiol. 278, C1031, 2000
Guinea-Pig Guinea-pig, adult, 250-350 g Smooth muscle
Gallbladder		 Papain: 0.1%
 NaCl, sodium glutamate, MgCl, KCl, glucose, Kreb's, and HEPES Jennings, L., Xu, Q., Firth, T., Nelson, M., and Mawe, G.: Cholesterol Inhibits Spontaneous Action Potentials and Calcium Currents in Guinea Pig Gallbladder Smooth Muscle, Am. J. Physiol. 277, G1017, 1999
Guinea-Pig Guinea-pig, Dunkin-Hartley, female Myocytes Protease:
DMEM Ryder, K., Bryant, S., and Hart, G.: Membrane Current Changes in Left Ventricular Myocytes Isolated From Guinea-Pigs After Abdominal Aortic Coarctation, Cardiovasc. Res. 27, 1278, 1993
Guinea-Pig Guinea-pig
(also rat, rabbit)		 Smooth muscle Trypsin: 0.1%
 Potassium buffer solution Hu, S., and Kim, H.: Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid, F.A.S.E.B. J. 6, A383, 1992
Guinea-Pig Guinea-pig, 200-300 g Smooth muscle
Mesenteric artery		 Collagenase: 0.3%
 CF solution Ohya, Y. and Sperelakis, N.: ATP Regulation of the Slow Calcium Channels in Vascular Smooth Muscle Cells, Circ. Res. 64, 145, 1989
Guinea-Pig Guinea-pig, 200-400 g
(also rabbit, frog, dogfish)		 Myocytes, heart and stomach Protease XIV: 0.028%
 Solution C
(see reference)		 Mitra, R. and Morad, M.: A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates, Am. J. Physiol. 249, H1056, 1985
Guinea-Pig Guinea-pig, prepubertal Smooth muscle
Aortic		 Trypsin: 0.05%
 Dulbecco-Vogt modification of Eagle's Ross, R.: The Smooth Muscle Cell . II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers, J. Cell Biol. 50, 172, 1971
Hamster Hamster, male, 60-70 day Satellite Trypsin: 0.25%
 DMEM Nakamura, T., Iwata, Y., Sampaolesi, M., Hanada, H., Saito, N., Artman, M., Coetzee, W., and Shigekawa, M.: Stretch-Activated Cation Channels in Skeletal Muscle Myotubes From Sarcoglycan-Deficient Hamsters, Am. J. Physiol. Cell Physiol. 281, C690, 2001
Human Human, female Smooth muscle
Myometrial		 Deoxyribonuclease I: 0.015% and 0.007%
 HBSS Richardson, M., Taylor, D., Casey, M., MacDonald, P., and Stull, J.: Biochemical Markers of Contraction in Human Myometrial Smooth Muscle Cells in Culture, In Vitro Cell. Dev. Biol. 23, 21, 1987
Human Human, female Smooth muscle
Myometrial		 Deoxyribonuclease I: 0.12%
 HBSS Casey, M., MacDonald, P., Mitchell, M., and Snyder, J.: Maintenance and Characterization of Human Myometrial Smooth Muscle Cells in Monolayer Culture, In Vitro 20, 396, 1984
Human Human, fetal
(also bovine)		 Smooth muscle, fibroblasts Trypsin: 0.055%
 DMEM Davies, P. and Kerr, C.: Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts, Biochim. Biophys. Acta 712, 26, 1982
Human Human Smooth muscle Trypsin: 0.25%
 DMEM Eskin, S., Sybers, H., Lester, J., Navarro, L., Gotto, A., and DeBakey, M.: Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall, In Vitro 17 (8), 713, 1981
Human Human
(also rat, guinea-pig, chick, monkey)		 Smooth muscle Trypsin: 0.05% - 0.1%
 HBSS Chamley-Campbell, J., Campbell, G., and Ross, R.: The Smooth Muscle in Cell Culture, Physiol. Res. 59, 1, 1979
Lizard Lizard (Anolis carolinensis) Myoblasts, tail Collagenase: 0.2%
 GM III
(see reference)		 Cox, P., and Simpson, Jr., S.: A Microphotometric STudy of Myogenic Lizard Cells Grown in Vitro, Dev. Biol. 23, 433, 1970
Monkey Monkey (Macaca nemestrina) Smooth muscle Trypsin: 0.05%
 Dulbecco-Vogt Chait, A., Ross, R., Albers, J., and Bierman, E.: Platelet-Derived Growth Factor Stimulates Activity of LDL Receptors, Proc. Natl. Acad. Sci. USA 77, 4084, 1980
Monkey Rhesus monkey, 1 year
(also human, rabbits)		 Smooth muscle, saphenous vein Elastase: 0.05%
 BSS Chamley, J., Campbell, G., McConnell, J., and Groschel-Stewart, U.: Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture, Cell Tissue Res. 177, 503, 1977
Mouse Mouse, 1-25 month Myocytes, endothelial Neutral Protease: 1.2 u/ml
Collagenase Type 4: 0.2%
 PBS Ieronimakis Nicholas, Balasundaram Gayathri, Reyes Morayma: Direct isolation, culture and transplant of mouse skeletal muscle derived endothelial cells with angiogenic potential, P.L.o.S. ONE 3, e0001753, 2008
Mouse Mouse, 15-20 day Intersitial cells of Cajal Collagenase Type 2: 0.13%
 M199 Li CX, Liu BH, Tong WD, Zhang LY, and Jiang YP: Dissociation, culture and morphologic changes of interstitial cells of Cajal in vitro, World J. Gastroenterol. 11, 2838, 2005
Mouse Mouse, 6-8 week Skeletal muscle progenitor Collagenase Type 2: 0.2%
 DMEM Majka S., Jackson K., Kienstra K., Majesky M., Goodell M., and Hirschi K.: Distinct Progenitor Populations in Skeletal Muscle Are Bone Marrow Derived and Exhibit Different Cell Fates During Vascular Regeneration, J. Clin. Invest. 111, 71, 2003
Mouse Mouse, neonatal Myocytes Collagenase Type 1: 0.5%
 DMEM Fukada S., Miyagoe-Suzuki Y., Tsukihara H., Yuasa K., Higuchi S., Ono S., Tsujikawa K., Takeda S., and Yamamoto H.: Muscle Regeneration by Reconstitution with Bone Marrow or Fetal Liver Cells from Green Fluorescent Protein-gene Transgenic Mice, J. Cell Sci. 115, 1285, 2002
Mouse Mouse, 6-8 week Myocytes Collagenase Type 2: 0.2%
Trypsin: 0.25%
 HBSS McKinney-Freeman SL, Jackson KA, Camargo FD, Ferrari G, Mavilio F, Goodell MA.: Muscle-derived hematopoietic stem cells are hematopoietic in origin, Proc. Natl. Acad. Sci. USA 99, 1341, 2002
Porcine Porcine, male, 25-40 kg Coronary myocytes Collagenase Type 2: 294 u/ml
Elastase: 6.5 u/ml
Deoxyribonuclease I: 0.04%
Soybean Trypsin Inhibitor: 0.1%
 low calcium physiological saline Korzick, D., Laughlin, M., and Bowles, D.: Alterations in PKC signaling underlie enhanced myogenic tone in exercise-trained porcine coronary resistance arteries, J. Appl. Physiol. 96, 1425-32, 2004
Porcine Porcine, adult, 35-45 kg Arterial smooth muscle Collagenase Type 2: 294 u/ml
Elastase: 6.5 u/ml
Deoxyribonuclease I: 0.4 mg/ml
Soybean Trypsin Inhibitor: 1 mg/ml
 MEM Wamhoff BR, Dixon JL, Sturek M: Atorvastatin treatment prevents alterations in coronary smooth muscle nuclear Ca2+ signaling in diabetic dyslipidemia, J. Vasc. Res. 39, 208, 2002
Porcine Porcine, Yorkshire, 30kg Coronary smooth muscle Collagenase Type 2: 150 u/ml
Elastase: 0.05%
 HBSS Sirous ZN, Fleming JB, Khalil RA: Endothelin-1 enhances eicosanoids-induced coronary smooth muscle contraction by activating specific protein kinase C isoforms, Hypertension 37, 497-504, 2001
Porcine Porcine Bladder smooth muscle Collagenase Type 2: 0.1-0.2%
 Krebs-Ringer bicarbonate Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M: Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells, J. Pharmacol. Exp. Ther. 296, 669-75, 2001
Porcine Porcine, juvenile, 30kg Coronary smooth muscle cells Collagenase: 0.3%
Elastase: 0.05%
 HBSS Huckle WR, Drag MD, Acker WR, Powers M, McFall RC, Holder DJ, Fujita T, Stabilito II, Kim D, Ondeyka DL, Mantlo NB, Chang RS, Reilly CF, Schwartz RS, Greenlee WJ, Johnson RG: Effects of subtype-selective and balanced angiotensin II receptor antagonists in a porcine coronary artery model of vascular restenosis, Circulation 93, 1009-19, 1996
Porcine Porcine Smooth muscle, aorta Collagenase: 0.3%
 DMEM Xiong, Yimin, Xu, Shangzhe, and Slakey, Linda L: Modulation of Response to Adenosine in Vascular Smooth Muscle Cells Cultured in Defined Medium, In Vitro Cell. Dev. Biol. 27, 355, 1991
Porcine Porcine Smooth muscle
Aorta		 Trypsin: 0.05%
 EDTA 0.02% Breton, M., Berrou, E., Deudon, E. and Picard, J.: Changes in Proteoglycans of Cultured Pig Aortic Smooth Muscle Cells During Subculture, In Vitro Cell. Dev. Biol. 26, 157, 1990
Porcine Porcine Smooth muscle
Aortic medial tissue		 Collagenase: 0.30%
 DMEM Fehr, T., Dickinson, E., Goldman, S. and Slakey, L.: Cyclic AMP Efflux is Regulated by Occupancy of The Adenosine Receptor in Pig Aortic Smooth Muscle Cells, J. Biol. Chem. 265, 10974, 1990
Quail Quail, embryo, 10 day Myoblasts Collagenase Type 2: 0.1%
 Puck's solution Konigsberg, I.: Skeletal Myoblasts in Culture, Methods Enzymol. LVIII, 511, 1979
Rabbit Rabbit, New Zealand White Aortic smooth muscle Collagenase Type 2: 300 u/ml
Elastase: 5 u/ml
 F10 Ham's Croons Valerie, Martinet Wim, Herman Arnold G, Timmermans Jean-Pierre, De Meyer Guido R Y: Selective clearance of macrophages in atherosclerotic plaques by the protein synthesis inhibitor cycloheximide, J. Pharmacol. Exp. Ther. 320, 986-93, 2007
Rabbit Rabbit, New Zealand, male, 2.5-3.5 Kg Enterocytes Trypsin: 0.1%
 RPMI 1640 w/ 1% fetal bovine serum
PBS		 Santos, M., Nguyen, B.., Thompson, J.: Factors Affecting in Vitro Growth of Harvested Enterocytes, Cell Transplant. 1, 299, 1992
Rabbit Rabbit
(also rat, guinea-pig)		 Smooth muscle Trypsin: 0.1%
 Potassium buffer solution Hu, S., and Kim, H.: Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid, F.A.S.E.B. J. 6, A383, 1992
Rabbit Rabbit, adult, 1-2 kg Smooth muscle Elastase: 0.17 - 0.25%
 Saline Benham, C., Bolton, T., Byrne, N., and Large, W.: Action of Extremely Applied Adenosine Triphosphate O Single Smooth Muscle Cells Dispersed From Rabbit Ear Artery, J. Physiol. 387, 473, 1987
Rabbit Rabbit, New Zealand white, 1500 g Smooth muscle, aortic Trypsin: 0.038%
 MEM Knodle, S., Anderson, S., and Papaioannou, S.: Large Scale Preparation of Rabbit Aortic Smooth Muscle Cells For Use in Calcium Uptake Studies, In Vitro Cell. Dev. Biol. 22, 23, 1986
Rabbit Rabbit, adult, 1-2 Kg Smooth muscle, ear artery Trypsin: 0.1%
 CF solution
(see reference)		 Benham, C.D., Bolton, T.B.: Spontaneous Transient Outward Currents in Single Visceral and Vascular Smooth Muscle Cells of the Rabbit, J. Physiol. 381, 385, 1986
Rabbit Rabbit, 0.5-1 Kg
(also guinea-pig, frog, dogfish)		 Myocytes, heart and stomach Protease XIV: 0.028%
 Solution C
(see reference)		 Mitra, R. and Morad, M.: A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates, Am. J. Physiol. 249, H1056, 1985
Rabbit Rabbit, white New Zealand, adult, male, 2 Kg Smooth muscle, aorta Trypsin: 0.1%
 Krebs Ringer HEPES solution Ives, H., Schultz, G., Galardy, R., and Jamieson, J.: Preparation of Functional Smooth Muscle Cells from the Rabbit Aorta, J. Exp. Med. 148, 1400, 1978
Rabbit Rabbits, New Zealand white albino, 5-6 months
(also human, Rhesus monkey, rabbit)		 Smooth muscle, saphenous vein Elastase: 0.05%
 BSS Chamley, J., Campbell, G., McConnell, J., and Groschel-Stewart, U.: Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture, Cell Tissue Res. 177, 503, 1977
Rabbit Rabbit, chinchilla, 5-8 month, virgin, female, 2-3 kg Smooth muscle, aorta Hyaluronidase: 800 u/ml
 HBSS Peters, T., Muller, M., and deDuve, C.: Lysosomes of the Arterial Wall. I. Isolation and Subcellular Fractionation of Cells from Normal Rabbit Aorta, J. Exp. Med. 136, 1117, 1972
Rabbit Rabbit, New England, albino Thoracic aorta Elastase: 0.008%
 Kreb's Ringer Day, A., Phil, D., and Newman, H.: Synthesis of Phospholipid by Foam Cells Isolated from Rabbit Atherosclerotic Lesions, Circ. Res. XIX, 122, 1966
Rat Rat, SD, adult and neonatal w/in 1 day of birth Myooids Neutral Protease: 4 u/ml
 Ham's F-12 Dennis, R., Kosnik II, P., Gilbert, M., and Faulkner, J.: Excitability and Contractility of Skeletal Muscle Engineered from Primary Cultures and Cell Lines, Am. J. Physiol. Cell Physiol. 280, C288, 2001
Rat Rat, SD, 3 month Smooth muscle cells Collagenase Type 2: 0.2%
Elastase: 0.04%
Soybean Trypsin Inhibitor: 0.1%
 M-199 Su E., Stevenson S., Rollence M., Marshall-Neff J., and Liau G.: A Genetically Modified Adenoviral Vector Exhibits Enhanced Gene Transfer of Human Smooth Muscle Cells, J. Vasc. Res. 38(5), 471, 2001
Rat Rat, SD, 250 g Arterial smooth muscle Papain: 0.03%
Collagenase: 0.1%
 see reference Jaggar JH: Intravascular pressure regulates local and global Ca(2+) signaling in cerebral artery smooth muscle cells, Am. J. Physiol. Cell Physiol. 281, C439-48, 2001
Rat Rat, Wistar, adult, male Smooth & skeletal muscle
Cardiac myocytes		 Protease: 0.01%
 PSS Wellman, G., Barrett-Jolley, R., Koppel, H. Everitt, D., and Quayle, J.: Inhibition of Vascular KATP Channels by U-37883A: A Comparison with Cardiac and Skeletal Muscle, Br. J. Pharmacol. 128, 909, 1999
Rat Rat, SD, male, 250-350 g Vascular smooth muscle Collagenase Type 2: 0.1%
Elastase: 0.0125%
 DMEM Hrometz, S., Edelmann, S., McCune, D., Olges, J., Hadley, R., Perez ,D., and Piascik, M.: Expression of Multiple Alpha1-Adrenoceptors on Vascular Smooth Muscle: Correlation with the Regulation of Contraction, J. Pharmacol. Exp. Ther. 290(1), 452, 1999
Rat Rat, SD, male, 150-175 g Smooth muscle, endothelial Trypsin: 0.04%
 MEM Redmond, E., Cahill, P., and Sitzmann, J.: Perfused Transcapillary Smooth Muscle and Endothelial Cell Co-Culture-A Novel In Vitro Model, In Vitro Cell. Dev. Biol. Anim. 31, 601, 1995
Rat Rat, Wistar Kyoto, 10-15 weeks Smooth muscle, mesenteric artery Trypsin: 0.05%
 MEM McGuire, P., Walker-Caprioglio, H., Little, S., and McGuffee, L.: Isolation and Culture of Rat Superior Mesenteric Artery Smooth Muscle Cells, In Vitro Cell. Dev. Biol. 29, 135, 1993
Rat Rat
(also rabbit, guinea-pig)		 Smooth muscle Trypsin: 0.1%
 Potassium buffer solution Hu, S., and Kim, H.: Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid, F.A.S.E.B. J. 6, A383, 1992
Rat Rat, SD, 19 days Smooth muscle, myometrial Trypsin: 150 µg/ml
 HBSS or PSS, CMF Loch-Caruso, R., Pahl, M., and Juberg, D.: Rat Myometrial Smooth Muscle Cells Show High Levels of Gap Junctional Communication Under a Variety of Culture Conditions, In Vitro Cell. Dev. Biol. 28, 97, 1992
Rat Rat, SHRs and WKY, male, 10 - 14 weeks Smooth muscle, tail arteries Papain: 0.1%
 HEPES buffer
(see reference)		 Bolzon, B. and Cheung, D.: Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats, Hypertension 14, 137, 1989
Rat Rat, 1-3 day Smooth muscle, aortic Elastase: 0.0125%
 DMEM Barone, L., Wolfe, L., Faris, B., and Franzblau, C.: Elastin mRNA Levels and Insoluble Elastin Accumulation in Neonatal Rat, Biochemistry 27, 3175, 1988
Rat Rat Muscle, mesenteric arteries Trypsin: 0.05%
 HEPES KG solution
(see reference)		 Bean, B., Sturek, M., Puga, A., and Hermsmeyer, K.: Calcium Channels in Muscle Cells Isolated From Rat Mesenteric Arteries: Modulation by Dihydropyridine Drugs, Circ. Res. 59, 229, 1986
Rat Rat, SHR, WKY. either sex, 12-19 day, 3 month, and retired breeders Endothelial, aortic Elastase: 0.05%
 Waymouth's culture medium Gordon, D., Mohai, L., and Schwartz, S.: Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells, Circ. Res. 59, 633, 1986
Rat Rat, male, 150-250 g Endothelial, aortic Trypsin:
RPMI 1640 Cole, O., Fan, T., and Lewis, G.: Isolation, Characterization, Growth and Culture of Endothelial Cells From the Rat Aorta, Cell. Biol. Int. Rep. 10 (6), 399, 1986
Rat Rat, Wistar, female, 10 weeks Myocytes Collagenase: 0.1%
 HBSS Boulanger-Saunier, C., Kattenburg, D., and Stoclet, J.: Cyclic AMP-dependent Phosphorylation of a 16kDa Protein in a Plasma Membrane-enriched Fraction of Rat Aortic Myocytes, F.E.B.S. Lett. 193, 283, 1985
Rat Rat, SD, male, 200 - 250 g Smooth muscle, thoracic aorta Trypsin: 0.0375%
 Eagle's MEM with calcium Brock, T., Alexander, R., Ekstein, L., Atkinson, W., and Gimbrone, M.: Angiotensin Increases Cytosolic Free Calcium in Cultured Vascular Smooth Muscle Cells, Hypertension 7, 105, 1985
Rat Rat, SD, 225-250g Mesenteric artery smooth muscle cells Elastase: .0125%
Soybean Trypsin Inhibitor: 0.025%
Collagenase Type 1: 0.1%
 HBSS Gunther S, Alexander RW, Atkinson WJ, and Gimbrone MA Jr.: Functional angiotensin II receptors in cultured vascular smooth muscle cells, J. Cell Biol. 92, 289, 1982
Rat Rat, 3-4 day Myocardial Trypsin NF 1:250: 0.125%
 HBSS CMF Kasten, F.: Rat Myocardial Cells In Vitro: Mitosis and Differentiated Properties, In Vitro 8, 128, 1972
Rat Rat, 200 g Muscle Trypsin: 0.05%
 Kreb's Henseleit bicarbonate buffer Kono, T.: Roles of Collagenases and Other Proteolytic Enzymes in the Dispersal of Animal Tissues, Biochim. Biophys. Acta 178, 397, 1969
Rat Rat, Wistar, 3-10 day Heart Trypsin NF 1:250: 250: 0.1%
 Saline A
(See reference)		 Harary, I., and Farley, B.: In Vitro Studies on Single Beating Rat Heart Cells, Exp. Cell Res. 29, 451, 1963
Sheep Sheep, adult and neonatal Tracheal smooth muscle cells Papain: 0.2%
Deoxyribonuclease I: 0.1%
 MOPS-PSS Driska S., Laudadio R., Wolfson M., and Shaffer T.: A Method for Isolating Adult and Neonatal Airway Smooth Muscle Cells and Measuring Shortening Velocity, J. Appl. Physiol. 86(1), 427, 1999