Miscellaneous - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Nucleus pulposus Collagenase Type 2: 0.2%
 DMEM Lee, J., Cheung, K. and Leung, V.: Systematic Study of Cell Isolation from Bovine Nucleus Pulposus: Improving Cell Yield and Experiment Reliability., J Orthop Res 33, 1743-55, 2015
Equine Equine Tendon Cells Collagenase Type 1: 0.1%
 DMEM Nemoto, M., Kizaki, K., Yamamoto, Y., Oonuma, T. and Hashizume, K.: Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration., J Equine Sci 24, 17-24, 2013
Goat Goat Infrapatellar fat pad mesenchymal stromal Collagenase: 0.15%
 DMEM Arora, A., Sriram, M., Kothari, A. and Katti, D.: Co-culture of Infrapatellar Fat Pad-Derived Mesenchymal Stromal Cells and Articular Chondrocytes in Plasma Clot for Cartilage Tissue Engineering., Cytotherapy 19, 881-894, 2017
Human Human Adhesion fibroblasts Collagenase Type 4: 0.2%
 Medium 199 Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation., Nat Commun 11, 4061, 2020
Human Human Nucleus pulposus Pronase: 0.25%
Collagenase Type 2: 600 u/ml
 DMEM Sun, Y., Lv, M., Zhou, L., Tam, V., Lv, F., Chan, D., Wang, H., Zheng, Z., Cheung, K. and Leung, V.: Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation., Osteoarthritis Cartilage 23, 1194-203, 2015
Human Human, 3-7 year Dental pulp stem cells Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM Salmon, B., Bardet, C, Khaddam, M. Naji, J., Coyac, B., Baroukh, B., Letourneur, F., Lesieur, J., Decup, F., Le Denmat, D., Nicoletti, A., Poliard, A, Rowe, P., Huet, E., Vital, S. and Linglart, A.: MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia., PLoS ONE 8, e56749, 2013
Human Human Dental pulp stem Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM/F12 Bonnamain, V., Thinard, R., Sergent-Tanguy, S., Huet, P., Bienvenu, G., Naveilhan, P., Farges, J. and Alliot-Licht, B.: Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium., Front Physiol 4, 357, 2013
Human Human Mesenchymal stromal Collagenase Type 1: 0.075%
 PBS Lee, Y., Lee, J., Park, H., Lim, Y., Lee, J., Wang, S. and Lee, B.: Isolation of Mesenchymal Stromal Cells (MSCs) from Human Adenoid Tissue., Cell Physiol Biochem 31, 513-24, 2013
Human Human Fungiform taste papillae Collagenase Type 1: 550 u/ml
Elastase: 10 u/ml
Soybean Trypsin Inhibitor: 0.09%
 see reference Ozdener, H., Spielman, A. and Rawson, N.: Isolation and Culture of Human Fungiform Taste Papillae Cells, J Vis Exp 63, e3730, 2012
Human Human Tendon cells Collagenase Type 2: 0,3%
 DMEM/Hams F12 Pauly, S., Klatte, F., Strobel, C., Schmidmaier, G., Greiner, S. and Scheibel, M. Wildemann B: Characterization of Tendon Cell Cultures of the Human Rotator Cuff., Eur Cell Mater 20, 84-97, 2010
Human Human Adipose derived stromal Collagenase Type 2: 0.075%
 DMEM Panetta, N., Gupta, D., Kwan, M., Wan, D., Commons, G. and Longaker, M.: Tissue Harvest by Means of Suction-Assisted or Third-Generation Ultrasound-Assisted Lipoaspiration Has No Effect on Osteogenic Potential of Human Adipose-Derived Stromal Cells., Plast Reconstr Surg 124, 65, 2009
Human Human Oral epithelial Neutral Protease: 1.2 u/ml
Trypsin: 0.05%
 PBS Nakamura, T., Endo, K. and Kinoshita, S.: Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling., Stem Cells 25, 628-38, 2007
Human Human Carotid artery plaque macrophage Collagenase Type 4: 450 u/ml
Deoxyribonuclease I: 500 u/ml
Soybean Trypsin Inhibitor: 0.1%
 HBSS Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM: Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin, Circ Res 98, 1282-9, 2006
Human Human Esophageal microvascular endothelial Collagenase Type 2: 0.2%
 MCDB-131 Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation., Am J Physiol Gastrointest Liver Physiol 285, G1277, 2003
Human Gastric epithelial cells Collagenase Type 2: 200 u/ml
Neutral Protease: 1.2 u/ml
Soybean Trypsin Inhibitor: 0.125%
 L-15 Smoot, D., Sewchand, J., Young, K., Desbrodes, B., Allen, C. and Naab, T.: A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells, Meth Cell Sci 22, 133, 2000
Human Human Synoviocytes Collagenase: 0.2%
 DMEM/F12 Chen V, Croft D, Purkis P, Kramer IM: Co-culture of synovial fibroblasts and differentiated U937 cells is sufficient for high interleukin-6 but not interleukin-1beta or tumour necrosis factor-alpha release., Br J Rheumatol 37, 148-56, 1998
Human Human Peptic cells Collagenase Type 4: 0.1%
Soybean Trypsin Inhibitor: 0.2%
 Ringer solution Lanas. A., Nerin, J., Esteva, F. and Sainz, R.: Non-Steroidal Anti-Inflammatory Drugs and Prostaglandin Effects on Pepsinogen Secretion by Dispersed Human Peptic Cells., Gut 36, 657-63, 1995
Human Human Periapical granuloma CLSPA: 0.25%
 RPMI-1640 Stern MH, Dreizen S, Mackler BF, Levy BM: Isolation and characterization of inflammatory cells from the human periapical granuloma, J Dent Res 61, 1408-12, 1982
Insect Insect, Lepidoptera Lepitopteran Collagenase Type 3: 0.35%
Hyaluronidase: 0.01%
 Dulbecco PBS Goodwin, R and McCawley, P: Initiating Attached Cell Lines From the Lepidoptera (Insecta), Meth Cell Sci 3, 567, 1977
Invertebrate Squid Mantle Collagenase: see reference
Papain: see reference
 Phosphate buffer Raman, M. and Mathew, S.: Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny., J Food Sci Technol 51, 1509-16, 2014
Mouse Mouse Organoid cultures Stemxyme 1: 0.1%
 PBS Huang, L., Desai, R., Conrad, D., Leite, N., Akshinthala, D., Lim, C., Gonzalez, R., Muthuswamy, L., Gartner, Z. and Muthuswamy, S.: Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids.,Cell Stem Cell Vol. 28,, , 1090-1104.e6, 2021
Mouse Mouse, embryonic Trachea Elastase: 0.1%
Neutral Protease: 0.25%
Deoxyribonuclease I: 0.01%
Trypsin: 0.125%
 DMEM/F12 He, M., Wu, B., Ye, W., Le, D., Sinclair, A., Padovano, V., Chen, Y., Li, K., Sit, R., Tan, M., Caplan, M., Neff, N., Jan, Y., Darmanis, S. and Jan, L.: Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway., Elife 9, 53085, 2020
Mouse Mouse Adhesion fibroblasts Collagenase Type 4: 0.2%
 Medium 199 Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation., Nat Commun 11, 4061, 2020
Mouse Mouse, 8-12 week Urinary bladder Collagenase Type 1: 50 u/ml
Collagenase Type 3: 40 u/ml
Papain: 45 u/ml
Deoxyribonuclease I: 4 u/ml
Collagenase Type 6: 20 u/ml
 Tyrode solution Atta, H., Gheinani, A., Wacker, A., Heshmati, Y., Bigger-Allen, A., Lambrinos, G., Gao, Y., Bielenberg, D. and Adam, R.: A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury., J Vis Exp , , 2020
Mouse Mouse Urothelial Collagenase Type 1: 0.5%
 PBS Huang, C., Chen, C. and Shyr, C.: The Anti-Tumor Effect of Intravesical Administration of Normal Urothelial Cells on Bladder Cancer., Cytotherapy 19, 1233-1245, 2017
Mouse Mouse Bone marrow stromal Collagenase Type 1: 0.2%
 PBS Ishida, T., Suzuki, S., Lai, C., Yamazaki, S., Kakuta, S., Iwakura, Y., Nojima, M., Takeuchi, Y., Higashihara, M., Nakauchi, H. and Otsu, M.: Pre-Transplantation Blockade of TNF-α-Mediated Oxygen Species Accumulation Protects Hematopoietic Stem Cells., Stem Cells 35, 989-1002, 2017
Mouse Mouse Cochlear Papain: 20 u/ml
Trypsin: 0.05%
 DMEM Kim, Y., Wang, S., Tymanskyj, S., Ma, L., Tao, H. and Zhang, L.: Dcc Mediates Functional Assembly of Peripheral Auditory Circuits., Sci Rep 6, 23799, 2016
Mouse Mouse Epithelial stem Collagenase: 2%
 DMEM/F12 Chavez, M., Hu, J., Seidel, K., Li, C., Jheon, A., Naveau, A., Horst, O. and Klein, O.: Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor., J Vis Exp , , 2014
Mouse Mouse, 26-30 g Myenteric plexus Collagenase Type 2: 0.13%
 Neurobasal A Smith, T., Grider, J., Dewey, W. and Akbarali, H.: Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels., PLoS ONE 7, e45251, 2012
Mouse Mouse, 10 week Synovial mesenchymal Collagenase: 0.1%
Deoxyribonuclease I: 0.005%
 DMEM Futami, I., Ishijima, M., Kaneko, H., Tsuji, K., Ichikawa-Tomikawa, N., Sadatsuki, R., Muneta, T., Arikawa-Hirasawa, E., Sekiya, I. and Kaneko, K.: Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium., PLoS ONE 7, e45517, 2012
Mouse Mouse Spleen, bone marrow endothelial Collagenase Type 4: 0.3-1.0%
Deoxyribonuclease I: 20 u/ml
 PBS Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands., Immunity 34, 590, 2011
Mouse Mouse Dentritic Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.001%
 DME Stock, A., Booth, S. and Cerundolo, V.: Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans., J Exp Med 208, 761, 2011
Mouse Mouse Cochleaer Trypsin: 0.125%
 DMEM/F-12 Jan, T., Chai, R., Sayyid, Z. and Cheng, A.: Isolating LacZ-Expressing Cells from Mouse Inner Ear Tissues Using Flow Cytometry., J Vis Exp 58, e3432, 2011
Mouse Mouse, 6-8 week Bone marrow mesenchymal stem Collagenase Type 1: 0.25%
 RPMI 1640 Xu, S., De Becker, A., Van Camp, B., Vanderkerken, K. and Van Riet, I.: An Improved Harvest and In Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells., J Biomed Biotechnol 2010, 105940, 2010
Mouse Mouse, 3-6 week Ear mesenchymal stem Collagenase Type 1: 0.2%
 DMEM/F12 Staszkiewicz, J., Gimble, J., Manuel, J. and Gawronska-Kozak, B.: IFATS Collection: Stem Cell Antigen-1-Positive Ear Mesenchymal Stem Cells Display Enhanced Adipogenic Potential., Stem Cells 26, 2666, 2008
Mouse Mouse, 6-10 week Salivary gland and stomach Collagenase Type 4: 0.8%
Deoxyribonuclease I: 1.0%
 RPMI-1640 Ji, H., Rintelen, F., Waltzinger, C., Meier, D, Bilancio, A., Pearce, W., Hirsch, E., Wymann, M., Ruckle, T., Camps, M., Vanhaesebroeck, B., Okkenhaug, K. and Rommel, C.: Inactivation of PI3Kgamma and PI3Kdelta Distorts T-cell Development and Causes Multiple Organ Inflammation., Blood 110, 2940, 2007
Mouse Mouse Bone marrow Collagenase Type 1: 0.15%
Neutral Protease: 0.15%
 PBS Bertoncello, I. and Williams, B.: Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining., Methods Mol Biol 263, 181, 2004
Mouse Mouse, 25-30 g Tracheal inflammatory cells Collagenase Type 4: 0.1%
Deoxyribonuclease I: 50 u/ml
Soybean Trypsin Inhibitor: 0.1%
 RPMI 1640 Minamoto Kanji, Pinsky DavidJ: Recipient iNOS but not eNOS deficiency reduces luminal narrowing in tracheal allografts, J Exp Med 196, 1321-33, 2002
Porcine Porcine, 6 month Synoviocytes Hyaluronidase: 660 u/ml
Trypsin: 0.25%
Collagenase Type 2: 583 u/ml
 DMEM Kean, T and Dennis, J.: Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions., PLoS ONE 10, e0129961, 2015
Porcine Porcine, <10 week Nucleus pulposus Collagenase: 0.05%
Pronase: 0.3%
 DMEM Potier, E. and Ito. K.: Can Notochordal Cells Promote Bone Marrow Stromal Cell Potential for Nucleus Pulposus Enrichment? A Simplified in Vitro System., Tissue Eng Part A 20, 3241-51, 2014
Porcine Porcine, various ages Nucleous pulposus and AF Pronase: 0.05%
Collagenase: 0.2%
 Modified F-12K Cho, H., Park, S., Lee, S., Kang, M., Hasty, K. and Kim, S.: Snapshot of Degenerative Aging of Porcine Intervertebral Disc: A Model to Unravel the Molecular Mechanisms., Exp Mol Med 43, 334-40, 2011
Rabbit Rabbit, New Zealand, 8-10 week Tenocytes and tendon stem cells Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM Zhang, J. and Wang, J.: Characterization of Differential Properties of Rabbit Tendon Stem Cells and Tenocytes., BMC Musculoskelet Disord 11, 10, 2010
Rat Rat, Lewis, M Synovial macrophages Collagenase Type 1: 250 u/ml
 DMEM Jain, S., Tran, T. and Amiji, M.: Macrophage Repolarization with Targeted Alginate Nanoparticles Containing IL-10 Plasmid DNA for the Treatment of Experimental Arthritis., Biomaterials 61, 162-77, 2015
Rat Rat, SD, 6-8 month Pluripotent tendon Collagenase Type 1: 0.3%
Neutral Protease: 0.4%
 DMEM Zhou, B., Zhou, Y. and Tang, K.: The Effects of Substance P on Pluripotent Tendon Cells: an In Vitro and In Vivo Study., J Musculoskelet Neuronal Interact 14, 349-58, 2014
Rat Rat, Wistar, 7-9 week Gingival mitochondria Collagenase Type 1: 0.115-0.130 %
 HBSS Kaneko, N., Rikimaru, T., Fujimura, T., Mori, S., Hidaka, S. and Kaya, H.: Preparation of Rat Gingival Mitochondria with an Improved Isolation Method., Int J Dent 2010, 275103, 2010
Rat Rat, male, 250-350g Submandibular acinar Collagenase: 0.05%
 PBS Silver, N., Proctor, GB, Arno, M. and Carpenter GH: Activation of mTOR Coincides with Autophagy During Ligation-induced Atrophy in the Rat Submandibular Gland., Cell Death Dis 1, e14, 2010
Rat Rat, SD, 8-10 week Oral fibroblasts Collagenase Type 1: 200 u/ml
 DMEM Lin, A., Hokugo, A., Choi, J. and Nishimura, I.: Small Cytoskeleton-Associated Molecule, Fibroblast Growth Factor Receptor 1 Oncogene Partner 2/Wound Inducible Transcript-3.0 (FGFR1OP2/wit3.0), Facilitates Fibroblast-Driven Wound Closure., Am J Pathol 176, 108-21, 2010
Rat Rat, 8-10 week Synovial cells Collagenase Type 1: 250 u/ml
 RPMI 1640 Moghaddami, M., Cleland, L. and Mayrhofer, G.: MHC II+ CD45+ Cells from Synovium-Rich Tissues of Normal Rats: Phenotype, Comparison with Macrophage and Dendritic Cell Lineages and Differentiation into Mature Dendritic Cells In Vitro., Int Immunol 17, 1103, 2005