Lung - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Pulmonary microvessel endothelial Collagenase Type 2: 1000 u/ml
 PBS Del Vecchio, P., Siflinger-Birnboim, A., Belloni, P., Holleran, L., Lum, H., and Malik, A.: Culture and Characterization of Pulmonary Microvascular Endothelial Cells, In Vitro Cell. Dev. Biol. 28A, 711, 1992
Guinea-Pig Guinea-pig, male, 250-300 g Alveolar type II Elastase: 40 u/ml
 PBS
(see reference)		 Sikpi, M., Nair, C., Johns, A., and Das, S.: Metabolic and Ultrastructural Characterizationof Guinea-Pig Alveolar Type II Cells Isolated by Centrifugal Elutriation, Biochim. Biophys. Acta 877, 20, 1986
Guinea-Pig Guinea-pig, male, 100 g Single cells Trypsin: 0.25%
 CF salt solution Phillips, H.: Dissociation of Single Cells from Lung or Kidney Tissue with Elastase, In Vitro 8, 101, 1972
Human Human Lung
Fibroblasts		 Trypsin: 0.05%
 DMEM Zhu, Y., Skold, C., Liu, X., Wang, H., Kohyama., T., Wen, F., Ertl, R., and Rennard, S.: Fibroblasts and Monocyte Macrophages Contract and Degrade Three-Dimensional Collagen Gels in Extended Co-Culture, Respir. Res. 2 (5), 295, 2001
Human Human, 16-24 week therapeutic abortions Alveolar type II
Fetal		 Trypsin: 50 ug/ml
 Ham's F-12, Eagle's MEM Liley, H., Ertsey, R., Gonzales, L., Odom, M., Hawgood, S., Dobbs, L., and Ballard, P.: Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung, Biochim. Biophys. Acta 961, 86, 1988
Human Human Epithelial Trypsin: 0.02%
 Medium 199 Lechner, J., Haugen, A., McClendon, I., and Pettis, E.: Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium, In Vitro 18 (7), 633, 1982
Human Human, 12-16 wk old embryo Fibroblasts Trypsin: 0.01%
 Eagle's MEM Kan, M., and Yamane, I.: In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium, J. Cell. Physiol. 111, 155, 1982
Human Human fetuses, 80 day
(also swine fetuses, 70 day, adult Amer Dutch, 250 day)		 Lung Collagenase: 0.01%
 HBSS Hinz, R., and Syverton, J.: Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase, Proc. Soc. Exp. Biol. Med. 101, 19, 1959
Mouse Mouse, 8-12 week Mononuclear cells Collagenase Type 1: 300 u/ml
Deoxyribonuclease I: 50 u/ml
 RPMI 1640 medium Woolard MD, Hodge LM, Jones HP, Schoeb TR, and Simecka JW: The upper and lower respiratory tracts differ in their requirement of IFN-gamma and IL-4 in controlling respiratory mycoplasma infection and disease, J. Immunol. 172, 6875, 2004
Mouse Mouse, 6-10 week Murine pulmonary endothelial Collagenase Type 2: 0.25%
 HBSS Andonegui G., Bonder C., Green F., Mullaly S., Zbytnuik L., Raharjo E., and Kubes P.: Endothelium-derived Toll-like Receptor-4 is the Key Molecule in LPS-induced Neutrophil Sequestration into Lungs, J. Clin. Invest. 111, 1011, 2003
Mouse Mouse Pulmonary T lymphocytes Collagenase Type 3: 10 mg/lung
Deoxyribonuclease I: 250 ug/lung
 RPMI medium Huaux F, Liu T, McGarry B, Ullenbruch M, and Phan SH.: Dual roles of IL-4 in lung injury and fibrosis, J. Immunol. 170, 2083, 2003
Mouse Mouse Alveolar epithelial cells Neutral Protease:
Deoxyribonuclease I: 0.01%
 DMEM Paine R 3rd, Wilcoxen SE, Morris SB, Sartori C, Baleeiro CE, Matthay MA, and Christensen PJ: Transgenic overexpression of granulocyte macrophage-colony stimulating factor in the lung prevents hyperoxic lung injury, Am. J. Pathol. 163, 2397, 2003
Mouse Mouse, 6-8 week Lung and lymph node cells Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.002%
 RPMI 1640 Vermaelen KY, Carro-Muino I, Lambrecht BN, and Pauwels RA.: Specific migratory dendritic cells rapidly transport antigen from the airways to the thoracic lymph nodes, J. Exp. Med. 193, 51, 2001
Mouse Mouse Lung Collagenase: 100 u/ml
Deoxyribonuclease I: 200 u/ml
 Krebs-Henseleit Buffer Freedman SD, Katz MH, Parker EM, Laposata M, Urman MY, and Alvarez JG.: A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr(-/-) mice, Proc. Natl. Acad. Sci. USA 96, 13995, 1999
Mouse Mouse, 6-8 wk Lung cells Collagenase Type 3: 150 u/ml
 HBSS Stampfli MR, Wiley RE, Neigh GS, Gajewska BU, Lei XF, Snider DP, Xing Z, Jordana M: GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice, J. Clin. Invest. 102, 1704-14, 1998
Mouse Mouse, female, 18-20 g Murine endothelial cells Collagenase Type 1: 1 mg/ml
 DMEM Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A,and Vecchi A.: A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants, Arterioscler. Thromb. Vasc. Biol. 17, 1599, 1997
Mouse Mouse, female Antigen presenting cells Collagenase: 150 u/ml
Deoxyribonuclease I: 30 u/ml
 RPMI 1640 Hamilton-Easton A, and Eichelberger M: Virus-specific antigen presentation by different subsets of cells from lung and mediastinal lymph node tissues of influenza virus-infected mice, J. Virol. 69, 6359, 1995
Porcine Swine fetuses (70 day), adult Amer Dutch 250 day
(also human fetuses, 80 day)		 Lung Collagenase: 0.01%
 HBSS Hinz, R., and Syverton, J.: Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase, Proc. Soc. Exp. Biol. Med. 101, 19, 1959
Rabbit Rabbit, New Zealand, white Alveolar type II Trypsin: 0.05%
 HBSS Scott, J.: The Role of Sera, Growth Factors, and Hormones in the In Vitro Production of Disaturated Phosphatidylcholine and Propagation of Undifferentiated Type II Alveolar Cells from the Fetal Rabbit Lung, Exp. Lung Res. 12, 181, 1987
Rabbit Rabbit, New Zealand white, adult,male Alveolar type II Trypsin: 0.0025%
 Joklik's MEM Finkelstein, J., Maniscalco, W., and Shapiro, D.: Properties of Freshly Isolated Type II Alveolar Epithelial Cells, Biochim. Biophys. Acta 762, 398, 1983
Rabbit Rabbit, New Zealand, male, 2-3 kg Clara cells Protease: 0.1%
 HEPES Devereux, T., and Fouts, J.: Isolation and Identification of Clara Cells From Rabbit Lung, In Vitro 16 (11), 958, 1980
Rabbit Rabbit, New Zealand, white, male, 1.7 Kg Lung Pronase: 0.2%
 Kreb's serum substitute solution, CMF Gould, M., Clements, J., Jones, A., and Felts, J.: Dispersal of Rabbit Lung into Individual Viable Cells: A New Model for the Study of Lung Metabolism, Science 178, 1209, 1972
Rat Rat, SD, 250-300g Alveolar epithelial Elastase: 3-4.5 u/ml
 RPMI 1640 Chen J., Chen Z., Narasaraju T., Jin N., and Liu L.: Isolation of Highly Pure Alveolar Epithelial Type I and Type II Cells from Rat Lungs, Lab. Invest. 84, 727, 2004
Rat Rat, SD, 300-400 g Pulmonary endothelial cells Collagenase Type 2: 1000 u/ml
 DMEM/F-12 King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F, and Stevens T: Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes, Microvasc. Res. 67, 139, 2004
Rat Rat, SD, 200-225 g Type II alveolar epithelial cells Elastase: 4.2 u/ml
Deoxyribonuclease I: 0.0001%
 DMEM Sunil VR, Connor AJ, Guo Y, Laskin JD, and Laskin DL: Activation of type II alveolar epithelial cells during acute endotoxemia, Am. J. Physiol. Lung Cell Mol. Physiol. 282, L872, 2002
Rat Rat, SD, male, adult Alveolar Elastase: 2.0 - 2.5 u/ml
 DMEM/Ham's F-12
(see reference)		 Kemp, P., Kim, K., Borok., Z., and Crandall, E.: Re-evaluating the Na+ Conductance of Adult Rat Alveolar Type II Pneumocytes: Evidence for the Involvement of cGMP-Activated Cation Channels, J. Physiol. 536 (3), 693, 2001
Rat Rat, Lewis, male, 200-250g Interstitial lung macrophages Collagenase Type 1: 100 u/ml
Deoxyribonuclease I: 50 u/ml
 RPMI-1640 Steinmuller C, Franke-Ullmann G, Lohmann-Matthes ML, Emmendorffer A: Local activation of nonspecific defense against a respiratory model infection by application of interferon-gamma: comparison between rat alveolar and interstitial lung macrophages, Am. J. Respir. Cell Mol. Biol. 22, 481-90, 2000
Rat Rat, male Alveolar epithelial Elastase: 40 u/ml
 DMEM Planus, E., Galiacy, S., Matthay, M., Laurent, V., Gavrilovic, J., Murphy, G., Clerici, C., Isabey, D., Lafuma, C., and d'Ortho, M.: Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair, J. Cell Sci. 112, 243, 1999
Rat Rat, male, 200-250g Pulmonary arterial myocytes Collagenase: 0.15%
Papain: 0.15%
Elastase: 0.05%
 PBS Bakhramov, A, Evans, M and Kozlowski, R: Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree, Exp. Physiol. 83, 337-47, 1998
Rat Rat, embryonic day 15 Fetal alveolar epithelial type II Trypsin: 0.1%
 DMEM Fraslon-Vanhulle, C, Bourbon, J and Batenburg, J: Culture of Fetal Alveolar Epithelial Type II Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 13A:2.1, 1995
Rat Rat, SD, male, 125-175 g Alveolar epithelial Elastase: 2 u/ml
 EBSS Kim, K., Suh, D., Lubman, R., Danto, S., Borok, Z., and Crandall, E.: Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers, J. Tiss. Cult. Meth. 14, 187, 1992
Rat Rat, Wistar Epithelial Trypsin: 0.1%
 HBSS Jassal, D., Han, R., Caniggia, I., Post, M., and Tanswell, A.: Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium, In Vitro Cell. Dev. Biol. 27A, 625, 1991
Rat Rat, SD, 8 day Interstitial Trypsin: 1.125%
 HEPES buffer Berk, J., Franzblau, C., and Goldstein, R.: Recombinant Interleukin-1beta Inhibits Elastin Formation by a Neonatal Rat Lung Fibroblast Subtype, J. Biol. Chem. 266, 3192, 1991
Rat Rat, Wistar, female, virgin Alveolar epithelial type II Trypsin: 1%
 Eagle's MEM Fraslon, C., Rolland, G., Bourbon, J., Rieutort, M., and Valenza, C.: Culture of Fetal Alveolar Epithelial Type II Cells in Serum-Free Medium, In Vitro Cell. Dev. Biol. 27A, 843, 1991
Rat Rat, Fischer 344, male Alveolar type II pneumocytes Elastase: 40 u/ml
 HEPES buffer
(see reference)		 Mangum, J., Everitt, J., Bonner, J., Moore, L., and Brody, A.: Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins, In Vitro Cell. Dev. Biol. 26, 1135, 1990
Rat Rat, SD, male, 250-400 g Alveolar type II Elastase: 40 u/ml
 Phosphate-buffered medium
(see reference)		 Ma, J., LaCagnin, L., Bowman, L., and Miles, P.: Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells, Biochim. Biophys. Acta 1003, 136, 1989
Rat Rat, Wistar, adult, male and pregnant female (known gestation) Alveolar type II Trypsin: 0.1%
 RPMI 1640 Batenburg, J., Otto-Verberne, C., Have-Opbroek, A., and Klazinga, W.: Isolation of Alveolar Type II Cells from Fetal Rat Lung by Differential Adherence in Monolayer Culture, Biochim. Biophys. Acta 960, 441, 1988
Rat Rat, SD, male, 150 - 200 g Pneumocytes type II Trypsin: 0.30%
 BSS Brown, L. and Longmore, W.: Altered Phospholipid Secretion in Type II Pneumocytes Isolated from Streptozotocin-diabetic Rats, Biochim. Biophys. Acta 878, 258, 1986
Rat Rat, SD, male, 180-200 g Alveolar type II Elastase: 4.3 u/ml
 HEPES
(see reference)		 Dobbs, L., Gonzalez R., and Williams, M.: An Improved Method for Isolating Type ll Cells in High Yield and Purity, Am. Rev. Respir. Dis. 134, 141, 1986
Rat Rat, SD, male Alveolar type I Trypsin: 0.05%
 DMEM Weller, N., and Karnovksy, M.: Isolation of Pulmonary Alveolar Type I Cells From Adult Rats, Am. J. Pathol. 124, 448, 1986
Rat Rat, fetus, 19 day Fibroblasts & type II Trypsin: 0.05%
 HBSS: serum free MEM, CMF Post, M., Torday, J., and Smith, B.: Alveolar Type II Cells Isolated from Fetal Rat Lung Organotypic Cultures Synthesize and Secrete Surfactant-Associated Phospholipids and Respond to Fibroblast-Pneumonocyte Factor, Exp. Lung Res. 7, 53, 1984
Rat Rat, SD, male, 150-400 g Alveolar type II Elastase: 4 u/ml
 Auto-Pow Eagle's modified MEM Goodman, B., Fleischer, R., and Crandall, E.: Evidence for Active Na+ Transport by Cultured Monolayers of Pulmonary Alveolar Epithelial Cells, Am. J. Physiol. 245, C78, 1983
Rat Rat, SD, male/female, 180-250 g Alveolar type II Trypsin: 0.30%
 BSS Mason, R., Williams, M., Greenleaf, R., and Clements, J.: Isolation and Properties of Type II Alveolar Cells from Rat Lung, Am. Rev. Respir. Dis. 115, 1015, 1977
Rat Rat, Wistar, pathogen free Alveolar type II Trypsin: 0.50%
 Earle's MEM King, R.: Metabolic Fate of the Apoproteins of Pulmonary Surfactant, Am. Rev. Respir. Dis. 115, 73, 1977
Rat Rat, fetus, 19 days Alveolar pneumonocytes, type II Trypsin: 0.1 %
 HBSS, CMF Douglas, W., and Teel, R.: An Organotypic in Vitro Model System for Studying Pulmonary Surfactant Production by Type II Alveolar Pneumonocytes, Am. Rev. Respir. Dis. 113, 17, 1976
Rat Rat, adult Lung Collagenase: 0.1%
 Moscona saline, CMF Douglas, W., and Kaighn, M.: Clonal Isolation of Differentiated Rat Lung Cells, In Vitro 10, 230, 1974
Rat Rat, SD, male, 100 g Alveolar type II Trypsin: 1.0%
 Joklik's medium Kikkawa, Y., and Yoneda, K.: Type II Epithelial Cell of the Lung. I. Method of Isolation, Lab. Invest. 30, 76, 1974