Lung - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Endothelial
Pulmonary		 Collagenase: 1000 u/ml
Collagenase Type 2: 1000 u/ml
 PBS, CMF Del Vecchio, P., Siflinger-Birnboim, A., Belloni, P., Holleran, L., Lum, H., and Malik, A.: Culture and Characterization of Pulmonary Microvascular Endothelial Cells, In Vitro Cell Dev Biol 28A, 711, 1992
Guinea-Pig Guinea-pig, male, 250-300 g Alveolar type II Elastase: 40 u/ml
 PBS
(see reference)		 Sikpi, M., Nair, C., Johns, A., and Das, S.: Metabolic and Ultrastructural Characterizationof Guinea-Pig Alveolar Type II Cells Isolated by Centrifugal Elutriation, Biochim Biophys Acta 877, 20, 1986
Guinea-Pig Guinea-pig, male, 100 g Single cells Trypsin: 0.25%
 CF salt solution Phillips, H.: Dissociation of Single Cells from Lung or Kidney Tissue with Elastase, In Vitro 8, 101, 1972
Human Human Aveolar Elastase: 12.9 u/ml
 PBS Bahmed, K., Boukhenouna, S., Karim, L., Andrews, T., Lin, J., Powers, R., Wilson, M., Lin, C., Messier, E., Reisdorph, N., Powell, R., Tang, H., Mason, R., Criner, G. and Kosmider, B.: The Effect of Cysteine Oxidation on DJ-1 Cytoprotective Function in Human Alveolar Type II Cells., Cell Death Dis 10, 638, 2019
Human Human Lung tumor Collagenase Type 1: 45-60 u/ml
Collagenase Type 2: 15-20 u/ml
Collagenase Type 4: 45-60 u/ml
Elastase: 0.002%
Deoxyribonuclease I: 0.002%
 DMEM/F12 Quatromoni, J., Singhal, S., Bhojnagarwala, P., Hancock, W., Albelda, S. and Eruslanov, E.: An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells, J Leukoc Biol 97, 201, 2015
Human Human Alveolar epithelial Neutral Protease: 2 u/ml
Trypsin: 0.25%
Elastase: 10 u/ml
 Bronchial Epithelial Growth Medium Barkauskas, C., Cronce, M., Rackley, C., Bowie, E., Keene, D., Stripp, B., Randell, S., Noble, P. and Hogan, B.: Type 2 Alveolar Cells are Stem Cells in Adult Lung, J Clin Invest 123, 3025, 2013
Human Human Pulmonary endothelial Collagenase Type 2: 0.2%
 RPMI Mackay, L., Dodd, S., Dougall, I., Tomlinson, W., Lordan, J., Fisher, A. and Corris, P.: Isolation and Characterisation of Human Pulmonary Microvascular Endothelial Cells from Patients with Severe Emphysema., Respir Res 14, 23, 2013
Human Human Lung endothelial Collagenase Type 2: 0.2%
 PBS Comhair, S., Xu, W., Mavrakis, L., Aldred, M., Asosingh, K. and Erzurum, S.: Human Primary Lung Endothelial Cells in Culture., Am J Respir Cell Mol Biol 46, 723-30, 2012
Human Human Lung Neutral Protease: 2 u/ml
Collagenase/Dispase: 0.1%
Deoxyribonuclease I: 0.01%
 DMEM Fujino, N., Kubo, H., Suzuki, T., Ota, C., Hegab, A., He, M., Suzuki, S., Suzuki, T., Yamada, M., Kondo, T., Kato, H. and Yamaya, M.: Isolation of Alveolar Epithelial Type II Progenitor Cells from Adult Human Lungs., Lab Invest 91, 363, 2011
Human Human Lung
Fibroblasts		 Trypsin: 0.05%
 DMEM Zhu, Y., Skold, C., Liu, X., Wang, H., Kohyama., T., Wen, F., Ertl, R., and Rennard, S.: Fibroblasts and Monocyte Macrophages Contract and Degrade Three-Dimensional Collagen Gels in Extended Co-Culture, Respir Res 2 (5), 295, 2001
Human Human, 16-24 week therapeutic abortions Alveolar type II
Fetal		 Trypsin: 50 ug/ml
 Ham's F-12, Eagle's MEM Liley, H., Ertsey, R., Gonzales, L., Odom, M., Hawgood, S., Dobbs, L., and Ballard, P.: Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung, Biochim Biophys Acta 961, 86, 1988
Human Human Epithelial Trypsin: 0.02%
 Medium 199 Lechner, J., Haugen, A., McClendon, I., and Pettis, E.: Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium, In Vitro 18 (7), 633, 1982
Human Human, 12-16 wk old embryo Fibroblasts Trypsin: 0.01%
 Eagle's MEM Kan, M., and Yamane, I.: In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium, J Cell Physiol 111, 155, 1982
Human Human fetuses, 80 day
(also swine fetuses, 70 day, adult Amer Dutch, 250 day)		 Lung Collagenase: 0.01%
 HBSS Hinz, R., and Syverton, J.: Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase, Proc Soc Exp Biol Med 101, 19, 1959
Mouse Mouse Fibroblasts Collagenase Type 2: 1.0%
Deoxyribonuclease I: 0.001%
 HBSS Qiu, Y., Xu, Y., Wang, M., Miao, M., Zhou, H., Xu, J., Kong, J., Zheng, D., Li, R., Zhang, R., Guo, Y., Li, X., Cui, J., Qin, C. and Zhou , X.: Flavivirus Induces and Antagonizes Antiviral RNA Interference in Both Mammals and Mosquitoes., Sci. Adv. 6, 7989, 2020
Mouse 2-3 month Lung endothelial Collagenase Type 1: 0.3%
 DMEM Wang, J., Niu, N., Xu, S. and Jin, Z.: A Simple Protocol for Isolating Mouse Lung Endothelial Cells., Sci Rep 9, 1458, 2019
Mouse Mouse, 6 week Alveolar epithelial Collagenase Type 1: 0.1%
Deoxyribonuclease I: 0.1%
Trypsin: 0.01%
 DMEM/F-12 Lewis, K., Hall, J., Kiyotake, E., Christensen, T., Balasubramaniam, V. and Anseth, K.: Epithelial-Mesenchymal Crosstalk Influences Cellular Behavior in a 3D Alveolus-Fibroblast Model System., Biomaterials 155, 124, 2018
Mouse Mouse Alveolar epithelial Neutral Protease: 0.1%
Deoxyribonuclease I: 0.01%
 DMEM Sun, F., Xiao, G. and Qu, Z.: Isolation of Murine Alveolar Type II Epithelial Cells., Bio-protocol 7, , 2017
Mouse Mouse Lung Collagenase Type 1: 0.15%
Deoxyribonuclease I: 0.04%
 PBS Yu, Y., O'Koren, E., Holten, D., Kan, M., Kojin, D., Nelson, E., Que, L. and Gunn, M.: A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflammed Murine Non-Lymphoid Tissues, PLoS ONE 10, 1371, 2016
Mouse Mouse Lung Collagenase Type 2: 0.3%
Deoxyribonuclease I: 0.3%
 PBS Braza, F., Dirou, S., Forest, V., Sauzeau, V., Hassoun, D., Chesne, J., Cheminant-Muller, M., Sagan, C., Magnan, A. and Lemarchand, P.: Mesenchymal Stem Cells Induce Suppressive Macrophages Through Phagocytosis in a Mouse Model of Asthma., Stem Cells 34, 1836-45, 2016
Mouse Mouse, 8-12 week Epithelial, Endothelial Collagenase Type 1: 0.5%
Deoxyribonuclease I: 0.1%
Neutral Protease: 0.1%
 PBS Singer, B., Mock, J., D'Alessio, F., Aggarwal, N., Mandke, P., Johnston, L. and Damarla, M.: Flow-Cytometric Method for Simultaneous Analysis of Mouse Lung Epithelial, Endothelial, and Hematopoietic Lineage Cells., Am J Physiol Lung Cell Mol Physiol 310, L796-801, 2016
Mouse Mouse Alveolar Collagenase Type 1: 300 u/ml
Elastase: 4 u/ml
Neutral Protease: 5 u/ml
Deoxyribonuclease I: 200 u/ml
 HBSS Mohapatra, A., Van Dyken, S., Schneider, C., Nussbaum, J., Liang, H. and Locksley, R.: Group 2 Innate Lymphoid Cells Utilize the IRF4-IL-9 Module to Coordinate Epithelial Cell Maintenance of Lung Homeostasis., Mucosal Immunol , , 2015
Mouse Mouse Pulmonary Collagenase Type 4: 0.16%
Deoxyribonuclease I: 0.1%
 see reference Kim, H., Lee, H., Chang, Y., Pichavant, M., Shore, S., Fitzgerald, K., Ikawura, Y., Israel, E., Bolger, K., Faul, J., DeKruyff, R. and Umetsu, D.: IL-17 Producing Innate Lymphoid Cells and the NLRP3 Inflammasone Facilitate Obescity-associated Airway Hyperractivity, Nat Med 20, 54, 2014
Mouse Mouse, embryonic Avelolar progenitor Elastase: 3 u/ml
Deoxyribonuclease I: 0.33 u/ml
 DMEM/F12 Desai, T., Brownfield, D. and Krasnow, M.: Alveolar Progenitor and Stem Cells in Lung Development, Renewal and Cancer., Nature 507, 190-4, 2014
Mouse Mouse Lung Collagenase Type 4: 0.3%
 PBS Guimond, D., Cam, N., Hirve, N., Duan, W., Lambris, J., Croft, M. and Tsoukas, C.: Regulation of Immune Responsiveness In Vivo by Disrupting an Early T-Cell Signaling Event Using a Cell-Permeable Peptide., PLoS ONE 8, e63645, 2013
Mouse Mouse Lung Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.01%
 HBSS Misharin, A., Morales-Nebreda, L., Mutlu, G., Budinger, G. and Perlman, H.: Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung., Am J Respir Cell Mol Biol 49, 503-10, 2013
Mouse Mouse, 8-12 week Lung progenitor Collagenase/Dispase: 0.2%
Neutral Protease: 5 u/ml
Deoxyribonuclease I: 0.0025%
 DMEM Driscoll, B., Kikuchi, A., Lau, A., Lee, J., Reddy, R., Jesudason, E., Kim, C. and Warburton, D.: Isolation and Characterization of Distal Lung Progenitor Cells., Methods Mol Biol 879, 109, 2012
Mouse Mouse Lung tumor Neutral Protease: 50 u/ml
Collagenase: 400 u/ml
Deoxyribonuclease I: 50 u/ml
 DMEM Vaughan, A., Halbert, C., Wootton, S. and Miller, A.: Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation., Mol Cancer Res 10, 86, 2012
Mouse Mouse Lung Collagenase Type 3: 0.1%
Deoxyribonuclease I: 0.0025%
 RPMI 1640 Hardy, C., LeMasurier, J., Belz, G., Scalzo-Inguanti, K., Yao, J., Xiang, S., Kanellakis, P., Bobik, A., Strickland, D., Rolland, J., O'Hehir, R. and Plebanski, M.: Inert 50-nm Polystyrene Nanoparticles That Modify Pulmonary Dendritic Cell Function and Inhibit Allergic Airway Inflammation., J Immunol 188, 1431, 2012
Mouse Mouse Lung draining Collagenase Type 4: 0.1-0.125%
 HBSS Rayamajhi, M., Redente, E., Condon, T., Gonzalez-Juarrero, M., Riches, D. and Lenz, L.: Non-Surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry., J Vis Exp 51, 2702, 2011
Mouse Mouse, 8-10 week Lung mesenchymal stem Collagenase Type 2: 0.2%
 HBSS Chow, K., Jun, D., Helm, K., Wagner, D. and Majka, S.: Isolation & Characterization of Hoechst(low) CD45(negative) Mouse Lung Mesenchymal Stem Cells., J Vis Exp 56, e3159, 2011
Mouse Mouse Dendritic Collagenase Type 1: 0.05%
Deoxyribonuclease I: 0.002%
 HBSS Lancelin, W. and Guerrero-Plata, A.: Isolation of Mouse Lung Dendritic Cells., J Vis Exp 57, e3563, 2011
Mouse Mouse Lung Collagenase Type 1: 450 u/ml
Elastase: 4 u/ml
Neutral Protease: 5 u/ml
Deoxyribonuclease I: 0.33 u/ml
 DMEM/F12 Rock, J, Barkauskas, C, Cronc, M, Xue, Y, Harris, J, Liang, J, Noble, P and Hogan, B.: Multiple Stromal Populations Contribute to Pulmonary Fibrosis Without Evidence for Epithelial to Mesenchymal Transition., Proc Natl Acad Sci U S A 108, E1475, 2011
Mouse Mouse, female, 6-12 week Lung mononuclear Collagenase Type 4: 500 u/ml
Deoxyribonuclease I: 0.002%
 HBSS Breslow, R., Rao, J., Xing, W., Hong, D., Barrett, N. and Katz, H.: Inhibition of Th2 Adaptive Immune Responses and Pulmonary Inflammation by Leukocyte Ig-Like Receptor B4 on Dendritic Cells., J Immunol 184, 1003, 2010
Mouse Mouse Lung Collagenase Type 2: 300 u/ml
Deoxyribonuclease I: 0.015%
 RPMI 1640 Ferreira, C., Antunes, F., Leonard, V., Welstead, G., Richardson, C. and Cattaneo, R.: Measles Virus Infection of Alveolar Macrophages and Dendritic Cells Precedes Spread to Lymphatic Organs in Transgenic Mice Expressing Human Signaling Lymphocytic Activation Molecule (SLAM, CD150)., J Virol 84, 3033, 2010
Mouse Mouse, embryo, day 18 Alveolar and fibroblast Trypsin: 2.5%
Deoxyribonuclease I: 0.2%
Collagenase Type 1: 1250 u/ml
 MEM Trotter, A., Kipp, M., Schrader, R. and Beyer, C.: Combined Application of 17Beta-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice., Int J Pediatr 2009, 170491, 2009
Mouse Mouse Lung Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.01%
 HBSS Zhao, J., Zhao, J., Van Rooijen, N. and Perlman, S.: Evasion by Stealth: Inefficient Immune Activation Underlies Poor T Cell Response and Severe Disease in SARS-CoV-Infected Mice., PLoS Pathog 5, e1000636, 2009
Mouse Mouse Dendritic Collagenase Type 1: 0.5%
 HBSS Flano, E., Jewell, N., Durbin, R. and Durbin, J.: Methods Used to Study Respiratory Virus Infection.,Curr Protoc Cell Biol Vol. Chapter 26,, , Unit 26.3, 2009
Mouse Mouse, 6-7 week Leukocytes and endothelial Collagenase: 0.2%
Deoxyribonuclease I: 40-80 u/ml
 PBS Jungblut, M., Oeltze, K., Zehnter, I., Hasselmann, D. and Bosio, A.: Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue Using the GentleMACS Dissociator., J Vis Exp 29, , 2009
Mouse Mouse Lung Collagenase Type 2: 0.2%
 PBS Jones, R., Capen, D., Cohen, K., Munn, L., Jain, R. and Duda, D.: A Protocol for Phenotypic Detection and Characterization of Vascular Cells of Different Origins in a Lung Neovascularization Model in Rodents., Nat Protoc 3, 388-97, 2008
Mouse Mouse, 18-20 g Lung Collagenase Type 3: 0.17%
 HBSS Ebeling, C., Lam, T., Gordon, J., Hollenberg, M. and Vliagoftis, H.: Proteinase-Activated Receptor-2 Promotes Allergic Sensitization to an Inhaled Antigen through a TNF-Mediated Pathway., J Immunol 179, 2910, 2007
Mouse Mouse Lung Collagenase Type 2: 300 u/ml
Deoxyribonuclease I: 0.001%
 Dulbecco's PBS Finotto, S., Eigenbrod, T., Karwot, R., Boross, I., Doganci, A., Ito, H., Nishimoto, N., Yoshizaki, K., Kishimoto, T., Rose-John, S., Galle, P. and Neurath, M.: Local Blockade of IL-6R Signaling Induces Lung CD4+ T Cell Apoptosis in a Murine Model of Asthma Via Regulatory T Cells., Int Immunol 19, 685, 2007
Mouse Mouse, 6-16 week Mononuclear Collagenase Type 4: 0.1%
 RPMI 1640 Abonia, J., Hallgren, J., Jones, T., Shi, T., Xu, Y., Koni, P., Flavell, R., Boyce, J., Austen, F. and Gurish, M.: Alpha-4 Integrins and VCAM-1, but not MAdCAM-1, are Essential for Recruitment of Mast Cell Progenitors to the Inflamed Lung., Blood 108, 1588-94, 2006
Mouse Mouse, 8-12 week Mononuclear cells Collagenase Type 1: 300 u/ml
Deoxyribonuclease I: 50 u/ml
 RPMI 1640 medium Woolard MD, Hodge LM, Jones HP, Schoeb TR, and Simecka JW: The upper and lower respiratory tracts differ in their requirement of IFN-gamma and IL-4 in controlling respiratory mycoplasma infection and disease, J Immunol 172, 6875, 2004
Mouse Mouse, 6-10 week Murine pulmonary endothelial Collagenase Type 2: 0.25%
 HBSS Andonegui G., Bonder C., Green F., Mullaly S., Zbytnuik L., Raharjo E., and Kubes P.: Endothelium-derived Toll-like Receptor-4 is the Key Molecule in LPS-induced Neutrophil Sequestration into Lungs, J Clin Invest 111, 1011, 2003
Mouse Mouse Pulmonary T lymphocytes Collagenase Type 3: 10 mg/lung
Deoxyribonuclease I: 250 ug/lung
 RPMI medium Huaux F, Liu T, McGarry B, Ullenbruch M, and Phan SH.: Dual roles of IL-4 in lung injury and fibrosis, J Immunol 170, 2083, 2003
Mouse Mouse Alveolar epithelial cells Neutral Protease:
Deoxyribonuclease I: 0.01%
 DMEM Paine R 3rd, Wilcoxen SE, Morris SB, Sartori C, Baleeiro CE, Matthay MA, and Christensen PJ: Transgenic overexpression of granulocyte macrophage-colony stimulating factor in the lung prevents hyperoxic lung injury, Am J Pathol 163, 2397, 2003
Mouse Mouse, 6-8 week Lung and lymph node cells Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.002%
 RPMI 1640 Vermaelen KY, Carro-Muino I, Lambrecht BN, and Pauwels RA.: Specific migratory dendritic cells rapidly transport antigen from the airways to the thoracic lymph nodes, J Exp Med 193, 51, 2001
Mouse Mouse Lung Collagenase: 100 u/ml
Deoxyribonuclease I: 200 u/ml
 Krebs-Henseleit Buffer Freedman SD, Katz MH, Parker EM, Laposata M, Urman MY, and Alvarez JG.: A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr(-/-) mice, Proc Natl Acad Sci U S A 96, 13995, 1999
Mouse Mouse, 6-8 wk Lung cells Collagenase Type 3: 150 u/ml
 HBSS Stampfli MR, Wiley RE, Neigh GS, Gajewska BU, Lei XF, Snider DP, Xing Z, Jordana M: GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice, J Clin Invest 102, 1704-14, 1998
Mouse Mouse, female, 18-20 g Murine endothelial cells Collagenase Type 1: 1 mg/ml
 DMEM Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A,and Vecchi A.: A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants, Arterioscler Thromb Vasc Biol 17, 1599, 1997
Mouse Mouse, female Antigen presenting cells Collagenase: 150 u/ml
Deoxyribonuclease I: 30 u/ml
 RPMI 1640 Hamilton-Easton A, and Eichelberger M: Virus-specific antigen presentation by different subsets of cells from lung and mediastinal lymph node tissues of influenza virus-infected mice, J Virol 69, 6359, 1995
Porcine Swine fetuses (70 day), adult Amer Dutch 250 day
(also human fetuses, 80 day)		 Lung Collagenase: 0.01%
 HBSS Hinz, R., and Syverton, J.: Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase, Proc Soc Exp Biol Med 101, 19, 1959
Rabbit Rabbit, New Zealand, white Alveolar type II Trypsin: 0.05%
 HBSS Scott, J.: The Role of Sera, Growth Factors, and Hormones in the In Vitro Production of Disaturated Phosphatidylcholine and Propagation of Undifferentiated Type II Alveolar Cells from the Fetal Rabbit Lung, Exp Lung Res 12, 181, 1987
Rabbit Rabbit, New Zealand white, adult,male Alveolar type II Trypsin: 0.0025%
 Joklik's MEM Finkelstein, J., Maniscalco, W., and Shapiro, D.: Properties of Freshly Isolated Type II Alveolar Epithelial Cells, Biochim Biophys Acta 762, 398, 1983
Rabbit Rabbit, New Zealand, male, 2-3 kg Clara cells Protease: 0.1%
 HEPES Devereux, T., and Fouts, J.: Isolation and Identification of Clara Cells From Rabbit Lung, In Vitro 16 (11), 958, 1980
Rabbit Rabbit, New Zealand, white, male, 1.7 Kg Lung Pronase: 0.2%
 Kreb's serum substitute solution, CMF Gould, M., Clements, J., Jones, A., and Felts, J.: Dispersal of Rabbit Lung into Individual Viable Cells: A New Model for the Study of Lung Metabolism, Science 178, 1209, 1972
Rat Rat Lung Collagenase Type 2: 0.2%
 PBS Jones, R., Capen, D., Cohen, K., Munn, L., Jain, R. and Duda, D.: A Protocol for Phenotypic Detection and Characterization of Vascular Cells of Different Origins in a Lung Neovascularization Model in Rodents., Nat Protoc 3, 388-97, 2008
Rat Rat, SD, 250-300g Alveolar epithelial Elastase: 3-4.5 u/ml
 RPMI 1640 Chen J., Chen Z., Narasaraju T., Jin N., and Liu L.: Isolation of Highly Pure Alveolar Epithelial Type I and Type II Cells from Rat Lungs, Lab Invest 84, 727, 2004
Rat Rat, SD, 300-400 g Pulmonary endothelial cells Collagenase Type 2: 1000 u/ml
 DMEM/F-12 King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F, and Stevens T: Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes, Microvasc Res 67, 139, 2004
Rat Rat, SD, 125-150 g Alveolar type I & II Elastase: 2.5-8 u/ml
Collagenase Type 1: 1.0%
 DMEM/F12 Liebler, J., Borok, Z., Li, X., Zhou, B., Sandoval, A., Kim, K.and Crandall, E.: Alveolar Epithelial Type I Cells Express Beta 2-Adrenergic Receptors and G-protein Receptor Kinase 2., J Histochem Cytochem 52, 759, 2004
Rat Rat, SD, 200-225 g Type II alveolar epithelial cells Elastase: 4.2 u/ml
Deoxyribonuclease I: 0.0001%
 DMEM Sunil VR, Connor AJ, Guo Y, Laskin JD, and Laskin DL: Activation of type II alveolar epithelial cells during acute endotoxemia, Am J Physiol Lung Cell Mol Physiol 282, L872, 2002
Rat Rat, SD, male, adult Alveolar Elastase: 2.0 - 2.5 u/ml
 DMEM/Ham's F-12
(see reference)		 Kemp, P., Kim, K., Borok., Z., and Crandall, E.: Re-evaluating the Na+ Conductance of Adult Rat Alveolar Type II Pneumocytes: Evidence for the Involvement of cGMP-Activated Cation Channels, J Physiol 536 (3), 693, 2001
Rat Rat, Lewis, male, 200-250g Interstitial lung macrophages Collagenase Type 1: 100 u/ml
Deoxyribonuclease I: 50 u/ml
 RPMI-1640 Steinmuller C, Franke-Ullmann G, Lohmann-Matthes ML, Emmendorffer A: Local activation of nonspecific defense against a respiratory model infection by application of interferon-gamma: comparison between rat alveolar and interstitial lung macrophages, Am J Respir Cell Mol Biol 22, 481-90, 2000
Rat Rat, male Alveolar epithelial Elastase: 40 u/ml
 DMEM Planus, E., Galiacy, S., Matthay, M., Laurent, V., Gavrilovic, J., Murphy, G., Clerici, C., Isabey, D., Lafuma, C., and d'Ortho, M.: Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair, J Cell Sci 112, 243, 1999
Rat Rat, male, 200-250g Pulmonary arterial myocytes Collagenase: 0.15%
Papain: 0.15%
Elastase: 0.05%
 PBS Bakhramov, A, Evans, M and Kozlowski, R: Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree, Exp Physiol 83, 337-47, 1998
Rat Rat, embryonic day 15 Fetal alveolar epithelial type II Trypsin: 0.1%
 DMEM Fraslon-Vanhulle, C, Bourbon, J and Batenburg, J: Culture of Fetal Alveolar Epithelial Type II Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 13A:2.1, 1995
Rat Rat, SD, male, 125-175 g Alveolar epithelial Elastase: 2 u/ml
 EBSS Kim, K., Suh, D., Lubman, R., Danto, S., Borok, Z., and Crandall, E.: Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers, J Tiss Cul Meth 14, 187, 1992
Rat Rat, Wistar Epithelial Trypsin: 0.1%
 HBSS Jassal, D., Han, R., Caniggia, I., Post, M., and Tanswell, A.: Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium, In Vitro Cell Dev Biol 27A, 625, 1991
Rat Rat, SD, 8 day Interstitial Trypsin: 1.125%
 HEPES buffer Berk, J., Franzblau, C., and Goldstein, R.: Recombinant Interleukin-1beta Inhibits Elastin Formation by a Neonatal Rat Lung Fibroblast Subtype, J Biol Chem 266, 3192, 1991
Rat Rat, Wistar, female, virgin Alveolar epithelial type II Trypsin: 1%
 Eagle's MEM Fraslon, C., Rolland, G., Bourbon, J., Rieutort, M., and Valenza, C.: Culture of Fetal Alveolar Epithelial Type II Cells in Serum-Free Medium, In Vitro Cell Dev Biol 27A, 843, 1991
Rat Rat, Fischer 344, male Alveolar type II pneumocytes Elastase: 40 u/ml
 HEPES buffer
(see reference)		 Mangum, J., Everitt, J., Bonner, J., Moore, L., and Brody, A.: Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins, In Vitro Cell Dev Biol 26, 1135, 1990
Rat Rat, SD, male, 250-400 g Alveolar type II Elastase: 40 u/ml
 Phosphate-buffered medium
(see reference)		 Ma, J., LaCagnin, L., Bowman, L., and Miles, P.: Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells, Biochim Biophys Acta 1003, 136, 1989
Rat Rat, Wistar, adult, male and pregnant female (known gestation) Alveolar type II Trypsin: 0.1%
 RPMI 1640 Batenburg, J., Otto-Verberne, C., Have-Opbroek, A., and Klazinga, W.: Isolation of Alveolar Type II Cells from Fetal Rat Lung by Differential Adherence in Monolayer Culture, Biochim Biophys Acta 960, 441, 1988
Rat Rat, SD, male, 150 - 200 g Pneumocytes type II Trypsin: 0.30%
 BSS Brown, L. and Longmore, W.: Altered Phospholipid Secretion in Type II Pneumocytes Isolated from Streptozotocin-diabetic Rats, Biochim Biophys Acta 878, 258, 1986
Rat Rat, SD, male, 180-200 g Alveolar type II Elastase: 4.3 u/ml
 HEPES
(see reference)		 Dobbs, L., Gonzalez R., and Williams, M.: An Improved Method for Isolating Type ll Cells in High Yield and Purity, Am Rev Respir Dis 134, 141, 1986
Rat Rat, SD, male Alveolar type I Trypsin: 0.05%
 DMEM Weller, N., and Karnovksy, M.: Isolation of Pulmonary Alveolar Type I Cells From Adult Rats, Am J Pathol 124, 448, 1986
Rat Rat, fetus, 19 day Fibroblasts & type II Trypsin: 0.05%
 HBSS: serum free MEM, CMF Post, M., Torday, J., and Smith, B.: Alveolar Type II Cells Isolated from Fetal Rat Lung Organotypic Cultures Synthesize and Secrete Surfactant-Associated Phospholipids and Respond to Fibroblast-Pneumonocyte Factor, Exp Lung Res 7, 53, 1984
Rat Rat, SD, male, 150-400 g Alveolar type II Elastase: 4 u/ml
 Auto-Pow Eagle's modified MEM Goodman, B., Fleischer, R., and Crandall, E.: Evidence for Active Na+ Transport by Cultured Monolayers of Pulmonary Alveolar Epithelial Cells, Am J Physiol 245, C78, 1983
Rat Rat, SD, male/female, 180-250 g Alveolar type II Trypsin: 0.30%
 BSS Mason, R., Williams, M., Greenleaf, R., and Clements, J.: Isolation and Properties of Type II Alveolar Cells from Rat Lung, Am Rev Respir Dis 115, 1015, 1977
Rat Rat, Wistar, pathogen free Alveolar type II Trypsin: 0.50%
 Earle's MEM King, R.: Metabolic Fate of the Apoproteins of Pulmonary Surfactant, Am Rev Respir Dis 115, 73, 1977
Rat Rat, fetus, 19 days Alveolar pneumonocytes, type II Trypsin: 0.1 %
 HBSS, CMF Douglas, W., and Teel, R.: An Organotypic in Vitro Model System for Studying Pulmonary Surfactant Production by Type II Alveolar Pneumonocytes, Am Rev Respir Dis 113, 17, 1976
Rat Rat, adult Lung Collagenase: 0.1%
 Moscona saline, CMF Douglas, W., and Kaighn, M.: Clonal Isolation of Differentiated Rat Lung Cells, In Vitro 10, 230, 1974
Rat Rat, SD, male, 100 g Alveolar type II Trypsin: 1.0%
 Joklik's medium Kikkawa, Y., and Yoneda, K.: Type II Epithelial Cell of the Lung. I. Method of Isolation, Lab Invest 30, 76, 1974