Lung - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Pulmonary microvessel endothelial Collagenase Type 2: 1000 u/ml
 PBS Culture and Characterization of Pulmonary Microvascular Endothelial Cells , In Vitro Cell Dev Biol 28A, 711, 1992
Guinea-Pig Guinea-pig, male, 250-300 g Alveolar type II Elastase: 40 u/ml
 PBS
(see reference)		 Metabolic and Ultrastructural Characterizationof Guinea-Pig Alveolar Type II Cells Isolated by Centrifugal Elutriation , Biochim Biophys Acta 877, 20, 1986
Guinea-Pig Guinea-pig, male, 100 g Single cells Trypsin: 0.25%
 CF salt solution Dissociation of Single Cells from Lung or Kidney Tissue with Elastase , In Vitro 8, 101, 1972
Human Human Lung Fibroblasts Trypsin: 0.05%
 DMEM Fibroblasts and Monocyte Macrophages Contract and Degrade Three-Dimensional Collagen Gels in Extended Co-Culture , Respir Res 2 (5), 295, 2001
Human Human, 16-24 week therapeutic abortions Alveolar type II Fetal Trypsin: 50 ug/ml
 Ham's F-12, Eagle's MEM Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung , Biochim Biophys Acta 961, 86, 1988
Human Human Epithelial Trypsin: 0.02%
 Medium 199 Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium , In Vitro 18 (7), 633, 1982
Human Human, 12-16 wk old embryo Fibroblasts Trypsin: 0.01%
 Eagle's MEM In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium , J Cell Physiol 111, 155, 1982
Human Human fetuses, 80 day
(also swine fetuses, 70 day, adult Amer Dutch, 250 day)		 Lung Collagenase: 0.01%
 HBSS Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase , Proc Soc Exp Biol Med 101, 19, 1959
Mouse mice, 8-12 week mononuclear cells Collagenase Type 1: 300 u/ml
Deoxyribonuclease I: 50 u/ml
 RPMI 1640 medium The upper and lower respiratory tracts differ in their requirement of IFN-gamma and IL-4 in controlling respiratory mycoplasma infection and disease. , J Immunol 172, 6875, 2004
Mouse mouse, 6-10 week murine pulmonary endothelial Collagenase Type 2: 0.25%
 HBSS Endothelium-derived Toll-like Receptor-4 is the Key Molecule in LPS-induced Neutrophil Sequestration into Lungs , J Clin Invest 111, 1011, 2003
Mouse mouse pulmonary T lymphocytes Collagenase Type 3: 10 mg/lung
Deoxyribonuclease I: 250 ug/lung
 RPMI medium Dual roles of IL-4 in lung injury and fibrosis. , J Immunol 170, 2083, 2003
Mouse mouse alveolar epithelial cells Neutral Protease:
Deoxyribonuclease I: 0.01%
 DMEM Transgenic overexpression of granulocyte macrophage-colony stimulating factor in the lung prevents hyperoxic lung injury. , Am J Pathol 163, 2397, 2003
Mouse mouse, 6-8 week lung and lymph node cells Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.002%
 RPMI 1640 Specific migratory dendritic cells rapidly transport antigen from the airways to the thoracic lymph nodes. , J Exp Med 193, 51, 2001
Mouse mouse Lung Collagenase: 100 u/ml
Deoxyribonuclease I: 200 u/ml
 Krebs-Henseleit Buffer A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr(-/-) mice. , Proc Natl Acad Sci U S A 96, 13995, 1999
Mouse mouse, 6-8 wk lung cells Collagenase Type 3: 150 u/ml
 HBSS GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice. , J Clin Invest 102, 1704-14, 1998
Mouse mouse, female, 18-20 g murine endothelial cells Collagenase Type 1: 1 mg/ml
 DMEM A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants. , Arterioscler Thromb Vasc Biol 17, 1599, 1997
Mouse mouse, female antigen presenting cells Collagenase: 150 u/ml
Deoxyribonuclease I: 30 u/ml
 RPMI 1640 Virus-specific antigen presentation by different subsets of cells from lung and mediastinal lymph node tissues of influenza virus-infected mice. , J Virol 69, 6359, 1995
Porcine Swine fetuses (70 day), adult Amer Dutch 250 day
(also human fetuses, 80 day)		 Lung Collagenase: 0.01%
 HBSS Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase , Proc Soc Exp Biol Med 101, 19, 1959
Rabbit Rabbit, New Zealand, white Alveolar type II Trypsin: 0.05%
 HBSS The Role of Sera, Growth Factors, and Hormones in the In Vitro Production of Disaturated Phosphatidylcholine and Propagation of Undifferentiated Type II Alveolar Cells from the Fetal Rabbit Lung , Exp Lung Res 12, 181, 1987
Rabbit Rabbit, New Zealand white, adult,male Alveolar type II Trypsin: 0.0025%
 Joklik's MEM Properties of Freshly Isolated Type II Alveolar Epithelial Cells , Biochim Biophys Acta 762, 398, 1983
Rabbit Rabbit, New Zealand, male, 2-3 kg Clara cells Protease: 0.1%
 HEPES Isolation and Identification of Clara Cells From Rabbit Lung , In Vitro 16 (11), 958, 1980
Rabbit Rabbit, New Zealand, white, male, 1.7 Kg Lung Pronase: 0.2%
 Kreb's serum substitute solution, CMF Dispersal of Rabbit Lung into Individual Viable Cells: A New Model for the Study of Lung Metabolism , Science 178, 1209, 1972
Rat rat, SD, 250-300g alveolar epithelial Elastase: 3-4.5 u/ml
 RPMI 1640 Isolation of Highly Pure Alveolar Epithelial Type I and Type II Cells from Rat Lungs , Lab Invest 84, 727, 2004
Rat rat, SD, 300-400 g pulmonary endothelial cells Collagenase Type 2: 1000 u/ml
 DMEM/F-12 Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes , Microvasc Res 67, 139, 2004
Rat rat, SD, 200-225 g type II alveolar epithelial cells Elastase: 4.2 u/ml
Deoxyribonuclease I: 0.0001%
 DMEM Activation of type II alveolar epithelial cells during acute endotoxemia , Am J Physiol Lung Cell Mol Physiol 282, L872, 2002
Rat Rat, SD, male, adult Alveolar Elastase: 2.0 - 2.5 u/ml
 DMEM/Ham's F-12
(see reference)		 Re-evaluating the Na+ Conductance of Adult Rat Alveolar Type II Pneumocytes: Evidence for the Involvement of cGMP-Activated Cation Channels , J Physiol 536 (3), 693, 2001
Rat Rat, Lewis, male, 200-250g interstitial lung macrophages Collagenase Type 1: 100 u/ml
Deoxyribonuclease I: 50 u/ml
 RPMI-1640 Local activation of nonspecific defense against a respiratory model infection by application of interferon-gamma: comparison between rat alveolar and interstitial lung macrophages. , Am J Respir Cell Mol Biol 22, 481-90, 2000
Rat rat, male alveolar epithelial Elastase: 40 u/ml
 DMEM Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair , J Cell Sci 112, 243, 1999
Rat Rat, male, 200-250g pulmonary arterial myocytes Collagenase: 0.15%
Papain: 0.15%
Elastase: 0.05%
 PBS Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree. , Exp Physiol 83, 337-47, 1998
Rat Rat, embryonic day 15 Fetal alveolar epithelial type II Trypsin: 0.1%
 DMEM , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 13A:2.1, 1995
Rat Rat, SD, male, 125-175 g Alveolar epithelial Elastase: 2 u/ml
 EBSS Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers, J Tiss Cul Meth 14, 187, 1992
Rat Rat, Wistar Epithelial Trypsin: 0.1%
 HBSS Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium , In Vitro Cell Dev Biol 27A, 625, 1991
Rat Rat, SD, 8 day Interstitial Trypsin: 1.125%
 HEPES buffer Recombinant Interleukin-1beta Inhibits Elastin Formation by a Neonatal Rat Lung Fibroblast Subtype , J Biol Chem 266, 3192, 1991
Rat Rat, Wistar, female, virgin Alveolar epithelial type II Trypsin: 1%
 Eagle's MEM Culture of Fetal Alveolar Epithelial Type II Cells in Serum-Free Medium , In Vitro Cell Dev Biol 27A, 843, 1991
Rat Rat, Fischer 344, male Alveolar type II pneumocytes Elastase: 40 u/ml
 HEPES buffer
(see reference)		 Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins , In Vitro Cell Dev Biol 26, 1135, 1990
Rat Rat, SD, male, 250-400 g Alveolar type II Elastase: 40 u/ml
 Phosphate-buffered medium
(see reference)		 Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells , Biochim Biophys Acta 1003, 136, 1989
Rat Rat, Wistar, adult, male and pregnant female (known gestation) Alveolar type II Trypsin: 0.1%
 RPMI 1640 Isolation of Alveolar Type II Cells from Fetal Rat Lung by Differential Adherence in Monolayer Culture , Biochim Biophys Acta 960, 441, 1988
Rat Rat, SD, male, 150 - 200 g Pneumocytes type II Trypsin: 0.30%
 BSS Altered Phospholipid Secretion in Type II Pneumocytes Isolated from Streptozotocin-diabetic Rats , Biochim Biophys Acta 878, 258, 1986
Rat Rat, SD, male, 180-200 g Alveolar type II Elastase: 4.3 u/ml
 HEPES
(see reference)		 An Improved Method for Isolating Type ll Cells in High Yield and Purity , Am Rev Respir Dis 134, 141, 1986
Rat Rat, SD, male Alveolar type I Trypsin: 0.05%
 DMEM Isolation of Pulmonary Alveolar Type I Cells From Adult Rats , Am J Pathol 124, 448, 1986
Rat Rat, fetus, 19 day Fibroblasts & type II Trypsin: 0.05%
 HBSS: serum free MEM, CMF Alveolar Type II Cells Isolated from Fetal Rat Lung Organotypic Cultures Synthesize and Secrete Surfactant-Associated Phospholipids and Respond to Fibroblast-Pneumonocyte Factor , Exp Lung Res 7, 53, 1984
Rat Rat, SD, male, 150-400 g Alveolar type II Elastase: 4 u/ml
 Auto-Pow Eagle's modified MEM Evidence for Active Na+ Transport by Cultured Monolayers of Pulmonary Alveolar Epithelial Cells , Am J Physiol 245, C78, 1983
Rat Rat, SD, male/female, 180-250 g Alveolar type II Trypsin: 0.30%
 BSS Isolation and Properties of Type II Alveolar Cells from Rat Lung , Am Rev Respir Dis 115, 1015, 1977
Rat Rat, Wistar, pathogen free Alveolar type II Trypsin: 0.50%
 Earle's MEM Metabolic Fate of the Apoproteins of Pulmonary Surfactant , Am Rev Respir Dis 115, 73, 1977
Rat Rat, fetus, 19 days Alveolar pneumonocytes, type II Trypsin: 0.1 %
 HBSS, CMF An Organotypic in Vitro Model System for Studying Pulmonary Surfactant Production by Type II Alveolar Pneumonocytes , Am Rev Respir Dis 113, 17, 1976
Rat Rat, adult Lung Collagenase: 0.1%
 Moscona saline, CMF Clonal Isolation of Differentiated Rat Lung Cells , In Vitro 10, 230, 1974
Rat Rat, SD, male, 100 g Alveolar type II Trypsin: 1.0%
 Joklik's medium Type II Epithelial Cell of the Lung. I. Method of Isolation , Lab Invest 30, 76, 1974