Lung - Tissue Tables
| Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
| Bovine
|
Bovine |
Pulmonary microvessel endothelial |
Collagenase Type 2: 1000 u/ml
|
PBS |
Del Vecchio, P., Siflinger-Birnboim, A., Belloni, P., Holleran, L., Lum, H., and Malik, A.: Culture and Characterization of Pulmonary Microvascular Endothelial Cells, In Vitro Cell Dev Biol
28A,
711, 1992 |
| Guinea-Pig
|
Guinea-pig, male, 250-300 g |
Alveolar type II |
Elastase: 40 u/ml
|
PBS
(see reference) |
Sikpi, M., Nair, C., Johns, A., and Das, S.: Metabolic and Ultrastructural Characterizationof Guinea-Pig Alveolar Type II Cells Isolated by Centrifugal Elutriation, Biochim Biophys Acta
877,
20, 1986 |
| Guinea-Pig
|
Guinea-pig, male, 100 g |
Single cells |
Trypsin: 0.25%
|
CF salt solution |
Phillips, H.: Dissociation of Single Cells from Lung or Kidney Tissue with Elastase, In Vitro 8,
101, 1972 |
| Human
|
Human |
Lung Fibroblasts |
Trypsin: 0.05%
|
DMEM |
Zhu, Y., Skold, C., Liu, X., Wang, H., Kohyama., T., Wen, F., Ertl, R., and Rennard, S.: Fibroblasts and Monocyte Macrophages Contract and Degrade Three-Dimensional Collagen Gels in Extended Co-Culture, Respir Res
2 (5),
295, 2001 |
| Human
|
Human, 16-24 week therapeutic abortions |
Alveolar type II Fetal |
Trypsin: 50 ug/ml
|
Ham's F-12, Eagle's MEM |
Liley, H., Ertsey, R., Gonzales, L., Odom, M., Hawgood, S., Dobbs, L., and Ballard, P.: Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung, Biochim Biophys Acta
961,
86, 1988 |
| Human
|
Human |
Epithelial |
Trypsin: 0.02%
|
Medium 199 |
Lechner, J., Haugen, A., McClendon, I., and Pettis, E.: Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium, In Vitro 18 (7),
633, 1982 |
| Human
|
Human, 12-16 wk old embryo |
Fibroblasts |
Trypsin: 0.01%
|
Eagle's MEM |
Kan, M., and Yamane, I.: In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium, J Cell Physiol
111,
155, 1982 |
| Human
|
Human fetuses, 80 day (also swine fetuses, 70 day, adult Amer Dutch, 250 day) |
Lung |
Collagenase: 0.01%
|
HBSS |
Hinz, R., and Syverton, J.: Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase, Proc Soc Exp Biol Med 101,
19, 1959 |
| Mouse
|
Mouse, 8-12 week |
Mononuclear cells |
Collagenase Type 1: 300 u/ml
Deoxyribonuclease I: 50 u/ml
|
RPMI 1640 medium |
Woolard MD, Hodge LM, Jones HP, Schoeb TR, and Simecka JW: The upper and lower respiratory tracts differ in their requirement of IFN-gamma and IL-4 in controlling respiratory mycoplasma infection and disease, J Immunol 172,
6875, 2004 |
| Mouse
|
Mouse, 6-10 week |
Murine pulmonary endothelial |
Collagenase Type 2: 0.25%
|
HBSS |
Andonegui G., Bonder C., Green F., Mullaly S., Zbytnuik L., Raharjo E., and Kubes P.: Endothelium-derived Toll-like Receptor-4 is the Key Molecule in LPS-induced Neutrophil Sequestration into Lungs, J Clin Invest 111,
1011, 2003 |
| Mouse
|
Mouse |
Pulmonary T lymphocytes |
Collagenase Type 3: 10 mg/lung
Deoxyribonuclease I: 250 ug/lung
|
RPMI medium |
Huaux F, Liu T, McGarry B, Ullenbruch M, and Phan SH.: Dual roles of IL-4 in lung injury and fibrosis, J Immunol 170,
2083, 2003 |
| Mouse
|
Mouse |
Alveolar epithelial cells |
Neutral Protease:
Deoxyribonuclease I: 0.01%
|
DMEM |
Paine R 3rd, Wilcoxen SE, Morris SB, Sartori C, Baleeiro CE, Matthay MA, and Christensen PJ: Transgenic overexpression of granulocyte macrophage-colony stimulating factor in the lung prevents hyperoxic lung injury, Am J Pathol 163,
2397, 2003 |
| Mouse
|
Mouse, 6-8 week |
Lung and lymph node cells |
Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.002%
|
RPMI 1640 |
Vermaelen KY, Carro-Muino I, Lambrecht BN, and Pauwels RA.: Specific migratory dendritic cells rapidly transport antigen from the airways to the thoracic lymph nodes, J Exp Med 193,
51, 2001 |
| Mouse
|
Mouse |
Lung |
Collagenase: 100 u/ml
Deoxyribonuclease I: 200 u/ml
|
Krebs-Henseleit Buffer |
Freedman SD, Katz MH, Parker EM, Laposata M, Urman MY, and Alvarez JG.: A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr(-/-) mice, Proc Natl Acad Sci U S A 96,
13995, 1999 |
| Mouse
|
Mouse, 6-8 wk |
Lung cells |
Collagenase Type 3: 150 u/ml
|
HBSS |
Stampfli MR, Wiley RE, Neigh GS, Gajewska BU, Lei XF, Snider DP, Xing Z, Jordana M: GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice, J Clin Invest 102,
1704-14, 1998 |
| Mouse
|
Mouse, female, 18-20 g |
Murine endothelial cells |
Collagenase Type 1: 1 mg/ml
|
DMEM |
Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A,and Vecchi A.: A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants, Arterioscler Thromb Vasc Biol
17,
1599, 1997 |
| Mouse
|
Mouse, female |
Antigen presenting cells |
Collagenase: 150 u/ml
Deoxyribonuclease I: 30 u/ml
|
RPMI 1640 |
Hamilton-Easton A, and Eichelberger M: Virus-specific antigen presentation by different subsets of cells from lung and mediastinal lymph node tissues of influenza virus-infected mice, J Virol 69,
6359, 1995 |
| Porcine
|
Swine fetuses (70 day), adult Amer Dutch 250 day (also human fetuses, 80 day) |
Lung |
Collagenase: 0.01%
|
HBSS |
Hinz, R., and Syverton, J.: Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase, Proc Soc Exp Biol Med 101,
19, 1959 |
| Rabbit
|
Rabbit, New Zealand, white |
Alveolar type II |
Trypsin: 0.05%
|
HBSS |
Scott, J.: The Role of Sera, Growth Factors, and Hormones in the In Vitro Production of Disaturated Phosphatidylcholine and Propagation of Undifferentiated Type II Alveolar Cells from the Fetal Rabbit Lung, Exp Lung Res 12,
181, 1987 |
| Rabbit
|
Rabbit, New Zealand white, adult,male |
Alveolar type II |
Trypsin: 0.0025%
|
Joklik's MEM |
Finkelstein, J., Maniscalco, W., and Shapiro, D.: Properties of Freshly Isolated Type II Alveolar Epithelial Cells, Biochim Biophys Acta
762,
398, 1983 |
| Rabbit
|
Rabbit, New Zealand, male, 2-3 kg |
Clara cells |
Protease: 0.1%
|
HEPES |
Devereux, T., and Fouts, J.: Isolation and Identification of Clara Cells From Rabbit Lung, In Vitro 16 (11),
958, 1980 |
| Rabbit
|
Rabbit, New Zealand, white, male, 1.7 Kg |
Lung |
Pronase: 0.2%
|
Kreb's serum substitute solution, CMF |
Gould, M., Clements, J., Jones, A., and Felts, J.: Dispersal of Rabbit Lung into Individual Viable Cells: A New Model for the Study of Lung Metabolism, Science 178,
1209, 1972 |
| Rat
|
Rat, SD, 250-300g |
Alveolar epithelial |
Elastase: 3-4.5 u/ml
|
RPMI 1640 |
Chen J., Chen Z., Narasaraju T., Jin N., and Liu L.: Isolation of Highly Pure Alveolar Epithelial Type I and Type II Cells from Rat Lungs, Lab Invest 84,
727, 2004 |
| Rat
|
Rat, SD, 300-400 g |
Pulmonary endothelial cells |
Collagenase Type 2: 1000 u/ml
|
DMEM/F-12 |
King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F, and Stevens T: Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes, Microvasc Res 67,
139, 2004 |
| Rat
|
Rat, SD, 200-225 g |
Type II alveolar epithelial cells |
Elastase: 4.2 u/ml
Deoxyribonuclease I: 0.0001%
|
DMEM |
Sunil VR, Connor AJ, Guo Y, Laskin JD, and Laskin DL: Activation of type II alveolar epithelial cells during acute endotoxemia, Am J Physiol Lung Cell Mol Physiol
282,
L872, 2002 |
| Rat
|
Rat, SD, male, adult |
Alveolar |
Elastase: 2.0 - 2.5 u/ml
|
DMEM/Ham's F-12
(see reference) |
Kemp, P., Kim, K., Borok., Z., and Crandall, E.: Re-evaluating the Na+ Conductance of Adult Rat Alveolar Type II Pneumocytes: Evidence for the Involvement of cGMP-Activated Cation Channels, J Physiol
536 (3),
693, 2001 |
| Rat
|
Rat, Lewis, male, 200-250g |
Interstitial lung macrophages |
Collagenase Type 1: 100 u/ml
Deoxyribonuclease I: 50 u/ml
|
RPMI-1640 |
Steinmuller C, Franke-Ullmann G, Lohmann-Matthes ML, Emmendorffer A: Local activation of nonspecific defense against a respiratory model infection by application of interferon-gamma: comparison between rat alveolar and interstitial lung macrophages, Am J Respir Cell Mol Biol 22,
481-90, 2000 |
| Rat
|
Rat, male |
Alveolar epithelial |
Elastase: 40 u/ml
|
DMEM |
Planus, E., Galiacy, S., Matthay, M., Laurent, V., Gavrilovic, J., Murphy, G., Clerici, C., Isabey, D., Lafuma, C., and d'Ortho, M.: Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair, J Cell Sci
112,
243, 1999 |
| Rat
|
Rat, male, 200-250g |
Pulmonary arterial myocytes |
Collagenase: 0.15%
Papain: 0.15%
Elastase: 0.05%
|
PBS |
Bakhramov, A, Evans, M and Kozlowski, R: Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree, Exp Physiol 83,
337-47, 1998 |
| Rat
|
Rat, embryonic day 15 |
Fetal alveolar epithelial type II |
Trypsin: 0.1%
|
DMEM |
Fraslon-Vanhulle, C, Bourbon, J and Batenburg, J: Culture of Fetal Alveolar Epithelial Type II Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
13A:2.1, 1995 |
| Rat
|
Rat, SD, male, 125-175 g |
Alveolar epithelial |
Elastase: 2 u/ml
|
EBSS |
Kim, K., Suh, D., Lubman, R., Danto, S., Borok, Z., and Crandall, E.: Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers, J Tiss Cul Meth
14,
187, 1992 |
| Rat
|
Rat, Wistar |
Epithelial |
Trypsin: 0.1%
|
HBSS |
Jassal, D., Han, R., Caniggia, I., Post, M., and Tanswell, A.: Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium, In Vitro Cell Dev Biol
27A,
625, 1991 |
| Rat
|
Rat, SD, 8 day |
Interstitial |
Trypsin: 1.125%
|
HEPES buffer |
Berk, J., Franzblau, C., and Goldstein, R.: Recombinant Interleukin-1beta Inhibits Elastin Formation by a Neonatal Rat Lung Fibroblast Subtype, J Biol Chem
266,
3192, 1991 |
| Rat
|
Rat, Wistar, female, virgin |
Alveolar epithelial type II |
Trypsin: 1%
|
Eagle's MEM |
Fraslon, C., Rolland, G., Bourbon, J., Rieutort, M., and Valenza, C.: Culture of Fetal Alveolar Epithelial Type II Cells in Serum-Free Medium, In Vitro Cell Dev Biol
27A,
843, 1991 |
| Rat
|
Rat, Fischer 344, male |
Alveolar type II pneumocytes |
Elastase: 40 u/ml
|
HEPES buffer
(see reference) |
Mangum, J., Everitt, J., Bonner, J., Moore, L., and Brody, A.: Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins, In Vitro Cell Dev Biol
26,
1135, 1990 |
| Rat
|
Rat, SD, male, 250-400 g |
Alveolar type II |
Elastase: 40 u/ml
|
Phosphate-buffered medium
(see reference) |
Ma, J., LaCagnin, L., Bowman, L., and Miles, P.: Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells, Biochim Biophys Acta
1003,
136, 1989 |
| Rat
|
Rat, Wistar, adult, male and pregnant female (known gestation) |
Alveolar type II |
Trypsin: 0.1%
|
RPMI 1640 |
Batenburg, J., Otto-Verberne, C., Have-Opbroek, A., and Klazinga, W.: Isolation of Alveolar Type II Cells from Fetal Rat Lung by Differential Adherence in Monolayer Culture, Biochim Biophys Acta
960,
441, 1988 |
| Rat
|
Rat, SD, male, 150 - 200 g |
Pneumocytes type II |
Trypsin: 0.30%
|
BSS |
Brown, L. and Longmore, W.: Altered Phospholipid Secretion in Type II Pneumocytes Isolated from Streptozotocin-diabetic Rats, Biochim Biophys Acta
878,
258, 1986 |
| Rat
|
Rat, SD, male, 180-200 g |
Alveolar type II |
Elastase: 4.3 u/ml
|
HEPES
(see reference) |
Dobbs, L., Gonzalez R., and Williams, M.: An Improved Method for Isolating Type ll Cells in High Yield and Purity, Am Rev Respir Dis 134,
141, 1986 |
| Rat
|
Rat, SD, male |
Alveolar type I |
Trypsin: 0.05%
|
DMEM |
Weller, N., and Karnovksy, M.: Isolation of Pulmonary Alveolar Type I Cells From Adult Rats, Am J Pathol 124,
448, 1986 |
| Rat
|
Rat, fetus, 19 day |
Fibroblasts & type II |
Trypsin: 0.05%
|
HBSS: serum free MEM, CMF |
Post, M., Torday, J., and Smith, B.: Alveolar Type II Cells Isolated from Fetal Rat Lung Organotypic Cultures Synthesize and Secrete Surfactant-Associated Phospholipids and Respond to Fibroblast-Pneumonocyte Factor, Exp Lung Res 7,
53, 1984 |
| Rat
|
Rat, SD, male, 150-400 g |
Alveolar type II |
Elastase: 4 u/ml
|
Auto-Pow Eagle's modified MEM |
Goodman, B., Fleischer, R., and Crandall, E.: Evidence for Active Na+ Transport by Cultured Monolayers of Pulmonary Alveolar Epithelial Cells, Am J Physiol 245,
C78, 1983 |
| Rat
|
Rat, SD, male/female, 180-250 g |
Alveolar type II |
Trypsin: 0.30%
|
BSS |
Mason, R., Williams, M., Greenleaf, R., and Clements, J.: Isolation and Properties of Type II Alveolar Cells from Rat Lung, Am Rev Respir Dis 115,
1015, 1977 |
| Rat
|
Rat, Wistar, pathogen free |
Alveolar type II |
Trypsin: 0.50%
|
Earle's MEM |
King, R.: Metabolic Fate of the Apoproteins of Pulmonary Surfactant, Am Rev Respir Dis 115,
73, 1977 |
| Rat
|
Rat, fetus, 19 days |
Alveolar pneumonocytes, type II |
Trypsin: 0.1 %
|
HBSS, CMF |
Douglas, W., and Teel, R.: An Organotypic in Vitro Model System for Studying Pulmonary Surfactant Production by Type II Alveolar Pneumonocytes, Am Rev Respir Dis 113,
17, 1976 |
| Rat
|
Rat, adult |
Lung |
Collagenase: 0.1%
|
Moscona saline, CMF |
Douglas, W., and Kaighn, M.: Clonal Isolation of Differentiated Rat Lung Cells, In Vitro 10,
230, 1974 |
| Rat
|
Rat, SD, male, 100 g |
Alveolar type II |
Trypsin: 1.0%
|
Joklik's medium |
Kikkawa, Y., and Yoneda, K.: Type II Epithelial Cell of the Lung. I. Method of Isolation, Lab Invest 30,
76, 1974 |
|
|