Liver - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Avian ducklings, 7 day hepatocytes Collagenase: 0.05%
Hyaluronidase: 0.05%
 DMEM/ F12 Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells , J Virol 75(16), 7651, 2001
Canine Dog, 13 years old
(also rat, guinea-pig, rabbit, human)		 Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Isolation And Culture of Adult Hepatocytes from Liver Biopsies , In Vitro 17, 935, 1981
Canine Dog, adult Hepatocytes Trypsin: 0.1%
 HBSS, CMF Continuous Culture of Normal Adult Mammalian Hepatocytes Exhibiting Parenchymal Functions , In Vitro 15, 120, 1979
Chicken Chicken, adult Hepatocytes Collagenase: 0.02%
 HEPES, CF Isolation and Long Term Maintenance of Differentiated Adult Chicken Hepatocytes in Primary Culture , In Vitro Cell Dev Biol 28, 615, 1992
Chicken Chicken
(also rat, SD, 150-250 g)		 Hepatocytes Collagenase Type 4: 6000 units
 Medium A Adhesion of Hepatocytes to Immobilized Sugars. A Threshold Phenomenon , J Cell Biol 254, 10830, 1979
Chicken Chicken, Leghorn, white, 10-15 day Hepatocytes Deoxyribonuclease I: 0.00125%
 PBS Lipogenesis and the Synthesis and Secretion of Very Low Density Lipoprotein by Avian Liver Cells in Nonproliferating Monolayer Culture , J Cell Biol 73, 332, 1974
Chicken Chick embryos, 5 day Heart Liver Trypsin: 3.0%
 Tyrode's solution, CMF "ECM": Its Nature, Origin, And Function in Cell Aggregation , Exp Cell Res 30, 257, 1963
Chicken Chick embryonic Various tissues (heart, liver, skeletal, cardiac) Trypsin: various grades
 CMF Tyrode's solution An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells , Exp Cell Res 16, 477, 1959
Equine equine, 4-13 yr hepatocytes Collagenase Type 4: 0.1%
 HBSS Preparation of Equine Isolated Hepatocytes , Toxicol In Vitro 17(5-6), 615, 2003
Fish Rainbow trout (Oncorhynchus mykiss), male & female,
120-600 g		 Hepatocytes Collagenase Type 2: 0.045%
 Kreb's-Ringer bicarbonate buffer, CF Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions , In Vitro Cell Dev Biol 26, 237, 1990
Fish Trout, male, 100 - 200 g Hepatocytes Collagenase Type 2: 0.045%
 HBSS with 0.05M HEPES Comparison of Trout Hepatocyte Culture on Different Substrates, In Vitro Cell Dev Biol 22, 360, 1986
Fish Rainbow trout, (Salmo gairdneri), male, 150-200 g Hepatocytes Collagenase: 100 u/ml
 HBSS/CMF Trout Hepatocyte Culture: Isolation and Primary Culture , In Vitro Cell Dev Biol 21, 221, 1985
Fish Trout 100g - 5kg Hepatocytes Hyaluronidase: 0.08%
 See reference Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner , Carcinogenesis 3, 511, 1982
Fish Trout (Salmo gairdneri), male, 150-200 g
Catfish (Ictalurus punctatus), male, 100 - 150 g		 Hepatocytes Collagenase: 100u/ml
 HBSS Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies, Natl Cancer Inst Monogr 65, 163, 1981
Guinea-Pig Guinea-pig, Hartley, male, 250-300 g Hepatocytes Collagenase Type 2:
Kreb's Ringer bicarbonate buffer Adrenergic Regulation of Glycogenolysis in Isolated Guinea-Pig Hepatocytes: Evidence that B2-Receptors Mediate Catecholamine Stimulation of Glycogenolysis , Arch Biochem Biophys 225, 196, 1983
Guinea-Pig Guinea-pig, young Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Isolation And Culture of Adult Hepatocytes from Liver Biopsies , In Vitro 17, 935, 1981
Human human hepatocytes Collagenase: 0.05%
 HEPES Human hepatocyte culture. , Methods Mol Biol 320, 283, 2006
Human Human hepatocytes Collagenase: 0.05%
 EBSS Isolation of Hepatocytes from Livers from Non-Heart-Beating Donors for Cell Transplantation , Liver Transpl 12, 713, 2006
Human human, adult hepatocytes Collagenase: 0.6%
 RPMI 1640   Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications. , Liver Int 24, 361, 2004
Human human hepatocytes Collagenase Type 4: 0.05%
 Williams medium E Effects of Dexamethasone on Aryl (SULT1A1)- and hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes , Drug Metab Dispos 30(9), 997, 2002
Human Human
(also rat)		 Hepatocytes Collagenase Type 1: 0.05%
 Leffert's buffer Repopulation of Mouse Liver with Human Hepatocytes and In Vivo Infection with Hepatitis B Virus , Hepatol 33, 981, 2001
Human Human Hepatocytes Collagenase: 0.05%
 HEPES buffer
(see reference)		 Culture of Human Hepatocytes From Small Surgical Liver Biopsies: Biochemical Characterization And Comparison With in vivo , In Vitro Cell Dev Biol 26, 67, 1990
Human Human, male, 17-40 yrs. Hepatocytes Collagenase: 0.05%
 HEPES buffer Differential Response of Primary Cultures of Human and Rat Hepatocytes to Aflatoxin B1-Induced Cytotoxicity and Protection by the Hepatoprotective agent(+)-Cyanidanol-3 , Biol Cell 63, 327, 1988
Human Human, 4 male kidney donors, age between 17 and 31
(also adult rats, male, SD, 180-200 g)		 Hepatocytes Collagenase: 0.05% & 0.025%
 HEPES
(see reference)		 Different Cytotoxicity and Metabolism of Doxorubicin, Daunorubicin, Epirubicin, Esorubicin, and Idarubicin in Cultured Human and Rat Hepatocytes , Biochem Pharmacol 37 (20), 3877, 1988
Human Human, 3 human kidney donors, age 20, 23, 25 Hepatocytes Collagenase: 0.05%
 HEPES High Yield Preparation of Isolated Human Adult Hepatocytes by Enzymatic Perfusion of the Liver , Cell Biol Int Rep 6 (6), 625, 1982
Human Human, 51-75 years old
(also rat, guinea-pig, rabbit, dog)		 Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Isolation And Culture of Adult Hepatocytes from Liver Biopsies , In Vitro 17, 935, 1981
Human Human Hepatocytes Trypsin: 0.1%
 HBSS, CMF Human Liver Cells, Tissue Culture Methods / Applications,(Kruse, P., Patterson, M. eds), , 54, 1973
Monkey Monkey (Macaca fascicularis), adult, 5-6 Kg Hepatocytes Trypsin: 160 u/ml
 HEPES buffer Isolation and Culture of Hepatocytes from the Cynogmolgus Monkey (Macaca fascicularis) , In Vitro Cell Dev Biol 26, 815, 1990
Mouse mouse hepatocytes Collagenase Type 1: 0.053%
 DMEM/ F-12 Systemically Delivered Antisense Oligomers Up-regulate Gene Expression in Mouse Tissues , Nat Biotechnol 20, 1228, 2002
Mouse mouse, 7 week Kupffer cells Collagenase Type 4: 0.05%
 HBSS Sex steroids regulate pro- and anti-inflammatory cytokine release by macrophages after trauma-hemorrhage. , Am J Physiol 277, C35, 1999
Mouse Mouse, C3H, 6-8 weeks Epithelial Collagenase: 0.10%
 DMEM Establishment of Epithelial Cell Lines from Mouse Regenerating Liver , In Vitro 15, 910, 1979
Mouse Mouse, 20-30 g Parenchymal and non-parenchymal Hyaluronidase: 0.1%
 Hank's w/ Insulin, CMF Glycolytic and Gluconeogenic Enzyme Activities in Parenchymal and Non-parenchymal Cells from Mouse Liver , Biochem J 126, 1009, 1972
Porcine piglets, one week hepatocytes Collagenase Type 4: 0.05%
 RPMI 1640 Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich , World J Gastroenterol 10, 699, 2004
Porcine pig, adult hepatocytes Collagenase: 0.05%
Dispase:
MEM Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System , Chin Med J 114, 946, 2001
Porcine Porcine, male, 6-40 kg Hepatocytes Collagenase: 0.8%
 PBS High Yield Hepatocyte Isolation from Pig Livers for Investigation of Hybrid Liver Support Systems: Influence of Collagenase Concentration and Body Weight , J Surg Res 62 (1), 85, 1996
Rabbit Rabbit, New Zealand white Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Isolation And Culture of Adult Hepatocytes from Liver Biopsies , In Vitro 17, 935, 1981
Rat rat, Lewis, female, 150-200 g hepatocytes Collagenase: see references
 DMEM Cryopreservation of isolated primary rat hepatocytes: enhanced survival and long-term hepatospecific function. , Ann Surg 241, 125, 2005
Rat rat, adult hepatocytes Collagenase Type 1: 0.1%
Pronase: 1%
Deoxyribonuclease I: 0.007%
 Williams E medium Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1. , Am J Pathol 164(4), 1347, 2004
Rat rat, wistar hepatocytes Collagenase Type 4: 0.05%
 RPMI 1640 Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich , World J Gastroenterol 10, 699, 2004
Rat Rat, SD, male, 230-250 g Hepatocytes Collagenase Type 1: 0.1%
 HBSS Enhanced Sythesis and Reduced Metabolism of Endothelin-1 (ET-1) by Hepatocytes - An Important Mechanism of Increased Endogenous Levels of ET-1 in Liver Cirrhosis , J Hepatol 33, 725, 2000
Rat rat, male, 3 week hepatocytes Collagenase Type 4: 0.02%
 Williams medium E Initiated Rat Hepatocytes in Primary Culture: A Novel Tool to Study Alterations in Growth Control During the First Stage of Carcinogenesis , Carcinogenesis 21, 79, 2000
Rat Rat, SD, male, 450-500 g Stellate Protease: 0.02%
 HBSS Down-Regulation of Endothelin Receptors by Transforming Growth Factor B1 in Hepatic Stellate Cells , J Hepatol 30, 440, 1999
Rat rat, Lewis, adult hepatocytes Collagenase: 60 u/ml
 HEPES Lecithin:retinol acyltransferase and retinyl ester hydrolase activities are differentially regulated by retinoids and have distinct distributions between hepatocyte and nonparenchymal cell fractions of rat liver , J Nutr 127, 218, 1997
Rat Rat Hepatocytes Collagenase: 0.04% - 0.06%
 PBS , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:14.1, 1995
Rat Rat Hepatocytes Collagenase: 0.05%
 DMEM , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:16.1, 1995
Rat Rat, SD, male, 200 g Hepatocytes Collagenase: 0.05%
 Medium 199 Cell-Extracellular Matrix Interactions Can Regulate the Switch between Growth and Differentiation in Rat Hepatocytes: Reciprocal Expression of C/EBPa and Immediate-Early Growth Response Transcription Factors , Mol Cell Biol 14 (9), 5858, 1994
Rat Rat, Wistar, adult, male, 100-125 g Hepatocytes Collagenase Type 2: 0.05%
 Serum-free medium Effects of Butyrate Homologes on Metallothionein Induction in Rat Primary Hepatocyte Cultures , In Vitro Cell Dev Biol 28, 320, 1992
Rat Rat, SD, male, 250-350 g Hepatocytes Collagenase Type 4: 200 u/ml
 RPMI 1640 Repeated Transplantation of Microencapsulated Hepatocytes for Sustained Correction of Hyperbilirubinemia in Gunn Rats , Cell Transplant 1, 275, 1992
Rat Rat, SD, 8 - 12 day Parenchymal hepatocytes Collagenase Type 4: 80 u/ml
 HBSS Toxicity Assessment of Papaverine Hydrochloride and Papaverine-Derived Metabolites in Primary Cultures of Rat Hepatocytes , In Vitro Cell Dev Biol 26, 515, 1990
Rat Rat, SD, male, 250-350 g Hepatocytes Collagenase Type 2: 0.05%
 HBSS, CMF Rat Primary Hepatocyte Cultures are a Good Model for Examining Metallothionein-Induced Tolerance to Cadmium Toxicity , In Vitro Cell Dev Biol 26, 75, 1990
Rat Rat, Wistar, male, 200 g Parenchymal Collagenase Type 1:
HEPES, modified The Effect of Vandate on Receptor-mediated Endocytosis of Asialoorosomucoid in Rat Liver Parenchymal Cells , J Biol Chem 265, 8999, 1990
Rat Rat, SD, male, 175-225 g Hepatocytes Collagenase:
Kreb's Ringer bicarbonate buffer Insulin Stimulates the Activity of a Protamine in Isolated Rat Hepatocytes , J Biol Chem 265, 7748, 1990
Rat Rat, SD, neonatal, 8 - 10 days Hepatocytes Collagenase Type 4: 0.05%
 Hanks' BSS, CF Protective Effect of Flavonoids on Drug-Induced Hepatotoxicity In Vitro , Toxicology 57, 267, 1989
Rat Rat, Fischer 344, male, 14 months Hepatocytes Collagenase: 0.05%
 Ringer's biocarbonate buffer Effect of Perfringolysin O on the Lateral Diffusion Constant of Membrane Proteins of Hepatocytes as Revealed by Fluorescence Recovery After Photobleaching , Biochim Biophys Acta 939, 551, 1988
Rat Rat, SD, male, 200-300 g Hepatocytes Collagenase: 0.05%
 HEPES Metabolism of 6,9,12-Octadecatetraenoic and 6,9,12,15-Octadecatetraenoic Acid , Biochim Biophys Acta 958, 153, 1988
Rat Rat, Wistar, male,3 month, 200 g Parenchymal Endothelial Kupffer Collagenase Type 1: 0.05%
 Krebs Henseleit Identification of Prostaglandin D2 as the Major Eiconsanoid from Liver Endothelial and Kupffer Cells , Biochim Biophys Acta 959, 143, 1988
Rat Rat, Wistar, male, 200 - 300 g Parenchymal Kupffer Collagenase Type 2: 0.05%
 HBSS with CaCl2 Effects of Monocyte Macrophages Stimulation on Hepatic Lipoprotein Receptors , Biochim Biophys Acta 958, 334, 1988
Rat Rat, Wistar, female, 17 days Hepatocytes Collagenase: 0.05%
 HBSS Induction of gamma-Glutamyl Transferase by Dexamethasone in Cultured Rat Hepatocytes , Enzyme 40, 212, 1988
Rat Rat, CD strain, albino, male, 140 -180 g Hepatocytes and Nonparenchymal Pronase: 0.1%
 HEPES buffer with calcium Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation , Proc Natl Acad Sci U S A 85, 1539, 1988
Rat Rat, 250-350 g
(also guinea-pig & rabbit)		 Hepatocytes Collagenase Type 2: 166 u/ml
 DMEM Hepatic Uptake of Asialoglycoprotein is Different Among Mammalian Species Due to Different Receptor Distribution , Biochim Biophys Acta 942, 57, 1988
Rat Rat, SD, male, 180-200 g Hepatocytes Collagenase: 0.05%
 MEM Modulation of Epidermal Growth Factor Receptors in Rat Hepatocytes by Two Liver-Promoting Regimens, a Choline-Deficient and a Phenobarbital Diet , Cancer Res 48, 1162, 1988
Rat Rat, Fisher 344, 150-200 g Bile ductular epithelial Collagenase Type 1: 220 u/ml
 Leibovitz L-15 Biochemical Characteristics of Hyperplastic Rat Bile Ductular Epithelial Cells Cultured "on Top" and "Inside" Different Extracellular Matrix Substitutes , Cancer Res 48, 6145, 1988
Rat Rat, Wistar, male, 260-310 g Hepatocytes Collagenase Type 1: 0.05%
 HEPES ATP-dependent inactivation and reactivation of constitutively recycling galactosyl receptors in isolated rat hepatocytes. , Biochem 27, 2061, 1988
Rat Rat, SD, male, 350-400 g Hepatocytes Collagenase Type 2:
HBSS Kinetics of Diacylglycerol Accumulation in Response to Vasopressin Stimulation in Hepatocytes of Continuously Endotoxaemic Rats , Biochem J 253, 73, 1988
Rat Rat, SD, male, 350 - 450 g Lipocytes Kupffer Sinusoidal endothelial Collagenase: 0.015%
 DMEM/Ham's F-12 Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan , Anal Biochem 161, 207, 1987
Rat Rat, SD, male Hepatocytes Collagenase: 100 - 200 µg/g body weight
 Eagle's
Eagle's w/HEPES
HBSS		 Monensin Inhibits Ligand Dissociation Only Transiently and Partially and distinguishes two galactosyl receptor pathways in isolated rat hepatocytes , J Cell Physiol 133, 243, 1987
Rat Rat, Fischer, male, 150-250 g Hepatocytes Collagenase Type 1: 125 - 250 u/ml
 MEM
(see reference)		 Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolation at Different Times after Partial Hepatectomy , Cancer Res 46, 1318, 1986
Rat Rat, SD, female, 100-180 g Hepatocytes Collagenase: 0.5%
 Krebs Ringer bicarbonate buffer Rapid Reciprocal Changes in Adrenergic Receptors in Intact Isolated Hepatocytes During Primary Cell Culture , Mol Pharmacol 27, 200, 1985
Rat Rat, SD, male, 270 - 320 g Hepatocytes Collagenase: 0.04%
 Bicarbonate buffer with calcium added Inhibition of Glucagon-Stimulated Hepatic Glycogenolysis by E-Series Prostaglandins , FEBS Lett 169, 293, 1984
Rat Rat, Wistar, adult, male, 150-200 g Hepatocytes Trypsin: 0.005%
 Williams medium E Degradation of Prostaglandin E2 in a Primary Culture of Adult Rat Hepatocytes , J Biochem (Tokyo) 96, 429, 1984
Rat Rat, Fischer, Lewis and SD, male 10-18 months Epithelial Trypsin: 0.05%
 CMF HBSS Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats , In Vitro 19, 576, 1983
Rat Rat, Wistar, male, 3 month old Endothelial Kupffer Parenchymal Collagenase: 0.05%
 HBSS In Vivo and in Vitro Uptake and Degradation of Acetylated Low Density Lipoprotein by Rat Liver Endothelial, Kupffer, and Parenchymal Cells , J Biol Chem 258 (20), 12221, 1983
Rat Rat, SD, male/female, 250-300 g Hepatocytes Collagenase: 100 u/ml
 Krebs Henseleit bicarbonate buffer Differences between Male and Female Rats in the Regulation of Hepatic Glycogenolysis. The Relative Role of Calcium and cAMP in Phosphorylase Activation by Catecholamines , J Biol Chem 257, 7987, 1982
Rat Rat, Wistar, 12 day Epithelial Trypsin: 0.05%
 HBSS, CMF Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels , Exp Cell Res 131, 363, 1981
Rat Rat, Zucker, obese Hepatocytes Collagenase Type 2: 0.30%
 Dulbecco-Vogt arginine free Eagle's Primary Cultures of Fetal Hepatocytes from the Genetically Obese Zucker Rat: Protein Synthesis , In Vitro 17, 651, 1981
Rat Rat, SD Hepatocytes Collagenase: 90 u/ml
 CF EGTA perfusate Isolation And Culture of Adult Hepatocytes from Liver Biopsies , In Vitro 17, 935, 1981
Rat Rat, Wistar, female, fetus Hepatocytes Collagenase: 0.025%
 HEPES buffer Changes in Some Chromatin and Cytoplastic Enzymes of Perinatal Rat Hepatocytes , In Vitro 16, 1, 1980
Rat Rat, SD, male, 250-300 g Parenchymal Collagenase Type 2: 0.05%
 Kreb's Henseleit bicarbonate buffer The Effects of Dexamethasone on Metabolic Activity of Hepatocytes in Primary Monolayer Culture , In Vitro 16, 559, 1980
Rat Rat, Wistar, male, 180-210 g Hepatocytes Hyaluronidase: 0.02%
 Kreb's buffer Separate Isolation of Cells from Nodules and Surrounding Parenchyma of the Same Precancerous Rat Liver: Biochemical and Cytochemical Characterization , Toxicology 18, 225, 1980
Rat Rat, SD, 7-10 day Liver Collagenase: 0.05%
 HBSS modified (see reference) , J Tiss Cul Meth 6, 35, 1980
Rat Rat, Wistar, male, 200-250 g Hepatocytes Hyaluronidase: 460 u/ml
 Saline Studies on Fatty Liver with Isolated Hepatocytes: II., The Action of Carbon Tetrachloride on Lipid Peroxidation, Protein and Triglyceride Synthesis and Secretion , Exp Mol Pathol 30, 116, 1979
Rat Rat, SD, female, 100-150 g Hepatocytes Collagenase Type 1: 0.065%
 DMEM
(see reference)		 Very Low Density Lipoprotein Synthesis and Secretion by Cultured Rat Hepatocytes , J Biol Chem 254 (6), 2010, 1979
Rat Rat
(also chicken)		 Hepatocytes Collagenase Type 3 & 4:
HEPES Adhesion of Hepatocytes to Immobilized Sugars , J Biol Chem 254 (21), 10830, 1979
Rat Rat, SD, male, 200 g Hepatocytes Collagenase Type 1: 100 u/ml
 Buffers 1 & 2
(see reference)		 Intercellular Adhesion between Juvenile Liver Cells.. A Method to Measure the Formation of Stable Lateral Contacts Between Cells Attached to a Collagen Gel , Exp Cell Res 109, 413, 1977
Rat Rat (WAG/RIJ), female, 24, 30, and 37 months Parenchymal Collagenase Type 1: 0.05% - 0.06%
 HEPES buffer Effect of Age on Protein Synthesis by Isolated Liver Parenchymal Cells , Mech Ageing Dev 6, 293, 1977
Rat Rat, Fisher, adult, male Hepatocytes Collagenase Type 1: 100 u/ml
 HBSS
(see reference)		 Rat Hepatocytes Primary Cell Cultures III. Improved Dissociation and Attachment Techniques and the Enchancement of Survival by Culture Medium , In Vitro 13 (12), 809, 1977
Rat Rat , Wistar, male, 250-300 g Hepatocytes Collagenase: 0.05%
 Hank's solution, CF Studies of Fatty Liver with Isolated Hepatocytes , Exp Mol Pathol 27, 339, 1977
Rat Rat, Fischer, male, adult, 170-265 g Hepatocytes Collagenase: 0.05%-0.10%
 Williams medium E Conditions Affecting Primary Cell Cultures of Functional Adult Rat Hepatocytes. I.. The Effect of Insulin , In Vitro 12, 521, 1976
Rat Rat, SD, male, 200-250 g Hepatocytes Collagenase Type 1: 0.05%
 MEM Insulin Regulation of Glycogen Synthase in the Isolated Rat Hepatocyte , Biochem Biophys Res Commun 69 (4), 997, 1976
Rat Rat, Fischer, adult, 200-250 g Epithelial Hyaluronidase: 0.0075%
 KCl-NaCl HEPES Buffer Long-Term Culture of Adult Rat Liver Epithelial Cells , Exp Cell Res 89, 139, 1974
Rat Rat, neonate, 3 day Hepatocytes Trypsin: 0.25%
 PBS, CMF A Diploid Rat Liver Cell Culture. 1. Characterization and Sensitivity to Aflatoxin B1 , In Vitro 9, 286, 1974
Rat Rat, SD, albino, male Parenchymal Hyaluronidase: 0.1%
 Hank's solution, CMF Primary Monolayer Cultures of Adult Rat Liver Parenchymal Cells Suitable for Study of the Regulation of Enzyme Synthesis , In Vitro 9, 399, 1974
Rat Rat, embryos, 1-3 days Hepatocytes Hyaluronidase: 0.10%
 HBSS The Isolation and Culture of Liver Cells , Methods Enzymol 32, 733, 1974
Rat Rat, SD, adult, male, 200-250 g Parenchymal Hyaluronidase: 0.10%
 HBSS, CF The fine structure, potassium content, and respiratory activity of isolated rat liver parenchymal cells prepared by improved enzymatic techniques , J Cell Biol 57, 642, 1973
Rat Rat, SD, adult, male, 180-300 g Parenchymal Collagenase Type 1: 0.05%
 Hank's solution, CF Parenchymal Cells from Adult Rat Liver in Nonproliferating Monolayer Culture. I. Functional Studies , J Cell Biol 59, 722, 1973
Rat Rat, Wistar, 200-250 g Hepatocytes Hyaluronidase: 0.10%
 HBSS, CF Induction of Tryptophan Oxygenase in Primary Rat Liver Cell Suspensions by Glucocorticoid Hormone , Exp Cell Res 72, 571, 1972
Rat Rat, SD, female, 130-160 g Hepatocytes Hyaluronidase: 0.08%
 HBSS, CF The Regulation of Gluconeogenesis in Isolated Rat Liver Cells by Glucagon, Insulin, Dibutyryl Cyclic Adenosine Monophosphate, and Fatty Acids , J Biol Chem 247, 3229, 1972
Rat Rat, Fisher 344, pregnant (19-21 days gestation),
200-250 g		 Hepatocytes Collagenase: 0.3%
 Modified Eagle's w/ Serum Studies on Primary Cultures of Differentiated Fetal Liver Cells , J Cell Biol 52, 559, 1972
Rat Rat, Wistar, male, 260-310 g Hepatocytes Collagenase: 0.01 - 0.08%
 HEPES Preparation of Rat Liver Cells , Exp Cell Res 74, 450, 1972
Rat Rat, Fisher, 10 day Epithelial-like Trypsin: 0.25%
 PBS Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver , Exp Cell Res 69, 106, 1971
Rat Rat, Wistar, male, 6-8 weeks, 80-160 G Hepatocytes Hyaluronidase: 1.0%
 HBSS, CMF Cultures from Adult Rat Liver Cells. 1.Establishment of Monolayer Cell-Cultures from Normal Lver , J Cell Physiol 78, 281, 1971
Rat Rat, 100-200 g Hepatocytes Hyaluronidase: 0.10%
 HBSS, CF Induction of Tyrosine Aminotransferase in Isolated Liver Cells , Biochim Biophys Acta 191, 161, 1969
Rat Rat, SD, adult, 200-300 g Parenchymal Hyaluronidase: 0.10%
Collagenase Type 1: 0.05%
 HBSS, CF High Yield Preparation of Isolated Rat Liver Parenchymal Cells , J Cell Biol 43, 506, 1969