Kidney - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Avian avian, house sparrows tubule segments Collagenase Type 2: 0.1%
 HBSS Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones , Am J Physiol 276, R847, 1999
Bovine Bovine Papillary duct Hyaluronidase: 0.2%
 Kreb's buffer
HEPES buffered saline		 Characteristics of Papillary Collecting Duct Cells in Primary Culture , Am J Physiol 255, F1160, 1988
Canine Dog, 1-5 months Proximal tubules Collagenase Type 1: 0.15%
 Krebs Ringer bicarbonate buffer Cystine Uptake By Cultured Cells Originating From Dog Proximal Tubule Segments , In Vitro Cell Dev Biol 26, 105, 1990
Canine Dog, mongrel, 20 Kg Proximal tubular Deoxyribonuclease I: 0.0125%
 (see reference) Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells , Can J Physiol Pharmacol 63, 417, 1985
Fish Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g		 Renal tubule Trypsin: 0.2%
 CMF solution
(see reference)		 Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport , Am J Physiol 251, 424, 1986
Guinea-Pig Guinea-pig, male, 100 g Single cells Trypsin: 0.25%
 CF salt solution Dissociation of Single Cells from Lung or Kidney Tissue with Elastase , In Vitro 8, 101, 1972
Hamster Hamster
(also rat, SD and Wistar, 150-225 g, rabbit, bovine)		 Papillary duct Hyaluronidase: 0.2%
 Kreb's buffer
HEPES buffered saline		 Characteristics of Papillary Collecting Duct Cells in Primary Culture , Am J Physiol 255, F1160, 1988
Human human, adult renal proximal tubule and cortical fibroblasts Collagenase Type 2: 383 u/ml
 DMEM/F-12 Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor. , J Pharmacol Exp Ther 289, 535-42, 1999
Human Human Renal cortex Trypsin: 0.1%
 Tissue Culture Grade Water , J Tiss Cul Meth 13, 143, 1991
Human Human Papillary duct Collagenase: 400 u/ml
 Eagle's MEM-HEPES buffer w/L-glutamine Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells , In Vitro Cell Dev Biol 26, 441, 1990
Human Human Mesangial Trypsin: 0.25%
 DMEM/Ham's F-12 Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells , Proc Natl Acad Sci U S A 85, 1642, 1988
Human Human, adult Tubular Collagenase: 250 u/ml
 PBS In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate , In Vitro Cell Dev Biol 23 (1), 34, 1987
Human Human, adult, 14-66 years Tubular Collagenase: 100 u/ml
 Joklik's MEM Isolation, Culture, and Charaterization of Human Renal Tubular Cells , J Urol 133, 324, 1985
Human Human Malignant Stromal Papain: 0.009%
 Sacks solution Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification , Cancer Res 40, 1043, 1980
Human Human, 24 newborn and stillborn
(also rabbit)		 Renal Trypsin: 0.25%
 See reference Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys , J Clin Microbiol 2 (3), 243, 1975
Monkey Monkey Kidney Trypsin: 0.01% - 0.00001%
 Eagle's MEM Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells , Dev Biol 37, 77, 1976
Mouse mouse, 6 week glomerular mesangial cells Collagenase Type 4: 0.1%
 RPMI 1640 Opposite regulation of type II and III receptors for immunoglobulin G in mouse glomerular mesangial cells and in the induction of anti-glomerular basement membrane (GBM) nephritis. , J Biol Chem 277(30), 27535, 2002
Mouse mouse, 3-7 month fibroblasts, mesangial, smooth muscle Trypsin: 0.25%
Collagenase: see reference
Soybean Trypsin Inhibitor: .05%
 DMEM Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation. , Mol Biol Cell 10, 1569-79, 1999
Mouse Mouse Epithelial Collagenase Type 4: 1%
 DMEM /F-12 , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley & Sons, Ltd., 14B:6.1, 1995
Mouse Mouse, naive SJL (H-2S) Proximal tubular epithelial Deoxyribonuclease I: 15 µg/ml
 RPMI 1640 Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis , J Cell Biol 107, 1359, 1988
Rabbit rabbit, New Zealand White, female nephron segments Collagenase Type 2: 0.05%
Deoxyribonuclease I: 50 u/ml
 Eagle's MEM A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments , Am J Physiol 273, F650, 1997
Rabbit Rabbit, New Zealand white, male, 1.5 - 2.0 Kg Duct Soybean Trypsin Inhibitor: 0.025%
 Hank's Solution with calcium and HEPES , J Tiss Cul Meth 13, 179, 1991
Rabbit Rabbit, New Zealand white, female, 2-3 Kg Renal proximal tubules Deoxyribonuclease I: 70 u/ml
 Modified DME-F12 Differences in Enzymatic and Mechanical Isolated Rabbit Renal Proximal Tubules: Comparison in Long-Term Incubation , In Vitro Cell Dev Biol 26, 898, 1990
Rabbit Rabbit
(also rat, SD and Wistar, 150-225 g, hamster, bovine)		 Papillary duct Hyaluronidase: 0.2%
 Kreb's buffer
HEPES buffered saline		 Characteristics of Papillary Collecting Duct Cells in Primary Culture , Am J Physiol 255, F1160, 1988
Rabbit Rabbit, New Zealand, white, 2-3 kg MTALH cells RCCT cells Collagenase: 0.1%
 DMEM Immunodissection of Cortical and Medullary Thick Ascending Limb Cells From Rabbit Kidney , Am J Physiol 255, F704, 1988
Rabbit Rabbit, New Zealand, white Collecting tubule Trypsin: 0.05%
 Kreb's Ringer buffer Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae, Prostaglandins 16, 759, 1978
Rabbit Rabbit, New Zealand, white, female, 2.5 Kg Papillary collecting duct Collagenase: 0.2%
 (see reference) Preparation of Renal Papillary Collecting Duct Cells for Study , Nat Neurosci 8, 191, 1975
Rat rat, SD, male, 200-250g medullary thick ascending limb Collagenase: 0.1%
 HEPES-saline Extracellular ATP stimulates NO production in rat thick ascending limb. , Hypertension 47, 563-7, 2006
Rat rat cortical tubule cells Collagenase: 0.6%
 DMEM Distribution and Oligomeric Association of Splice Forms of Na(+)-K(+)-ATPase Regulatory Gamma-subunit in Rat Kidney , Am J Physiol/Renal 282(3), F393, 2002
Rat rat, SD, 1 week cortical collection duct Collagenase Type 2: 0.15%
 DMEM/Ham's F12 Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture , Am J Physiol Cell Physiol 280(5), C1193, 2001
Rat Rat, SD, 250-300 g Microvessels Collagenase Type 2: 0.1%
 PSS Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels , Microvasc Res 60, 149, 2000
Rat rat, SD, 250-300 g renal Collagenase Type 2: 0.2%
 see reference Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature , Hypertension 35, 337, 2000
Rat rat, SD, male, 5-6 week renal tubules Collagenase: 0.1%
 see reference Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney , Am J Physiol 277, F437, 1999
Rat rat, SD, male nephron segments Collagenase Type 2: 0.05%
Deoxyribonuclease I: 50 u/ml
 Eagle's MEM A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments , Am J Physiol 273, F650, 1997
Rat Rat, 12-weeks-old Renal Collagenase: 0.1%
 DMEM Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein , Nat Neurosci 50, 1506, 1996
Rat Rat, male, 150-200 g Epithelial Proximal tubule Protease: 0.1%
 HBSS/CMF Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury , In Vitro Cell Dev Biol 27, 739, 1991
Rat Rat, adult Inner medullary collecting duct Papillae Collagenase Type 2: 0.1%
 PBS Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells , Am J Physiol 257, F486, 1989
Rat Rat, Wistar, female, 100-150 g Glomerular mesangial Collagenase: 0.025%
 RPMI 1640 The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells , Biochim Biophys Acta 969, 217, 1988
Rat Rat, Wistar, male, 150 - 200 g Renal target Collagenase: 1.0% (also 0.7%)
 Eagle's MEM Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine , Proc Natl Acad Sci U S A 85, 1707, 1988
Rat Rat, Wistar, male, adult Proximal tubules Collagenase: 0.2%
 Krebs-Henseleit buffer Improved Separation Method for Rat Proximal and Distal Renal Tubules , Am J Physiol 253, F358, 1987
Rat Rat,Wistar, 300-400 gm Proximal tubules Collagenase: 0.15 %
 Krebs Henseleit solution Isolation of a Pure Suspension of Rat Proximal Tubules , Am J Physiol 241, F403, 1981
Rat Rat Fibroblasts Kidney Trypsin: up to 0.25%
 Dulbecco-Vogt MEM Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin , J Biol Chem 253, 24, 1978