Kidney - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Avian Avian, house sparrows Tubule segments Collagenase Type 2: 0.1%
 HBSS Goldstein D., Reddy V., and Plaga K.: Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones, Am. J. Physiol. 276, R847, 1999
Bovine Bovine Papillary duct Hyaluronidase: 0.2%
 Kreb's buffer
HEPES buffered saline		 Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am. J. Physiol. 255, F1160, 1988
Canine Dog, 1-5 months Proximal tubules Collagenase Type 1: 0.15%
 Krebs Ringer bicarbonate buffer States, B., Reynolds, R., Lee, J., and Segal, S.: Cystine Uptake By Cultured Cells Originating From Dog Proximal Tubule Segments, In Vitro Cell. Dev. Biol. 26, 105, 1990
Canine Dog, mongrel, 20 Kg Proximal tubular Deoxyribonuclease I: 0.0125%
 (see reference) Yau, C., Rao, L., and Silverman, M.: Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells, Can. J. Physiol. Pharmacol. 63, 417, 1985
Fish Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g		 Renal tubule Trypsin: 0.2%
 CMF solution
(see reference)		 Dickman, K. and Renfro, J.: Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport, Am. J. Physiol. 251, 424, 1986
Guinea-Pig Guinea-pig, male, 100 g Single cells Trypsin: 0.25%
 CF salt solution Phillips, H.: Dissociation of Single Cells from Lung or Kidney Tissue with Elastase, In Vitro 8, 101, 1972
Hamster Hamster
(also rat, SD and Wistar, 150-225 g, rabbit, bovine)		 Papillary duct Hyaluronidase: 0.2%
 Kreb's buffer
HEPES buffered saline		 Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am. J. Physiol. 255, F1160, 1988
Human Human, adult Renal proximal tubule and cortical fibroblasts Collagenase Type 2: 383 u/ml
 DMEM/F-12 Johnson, D., Saunders, H., Johnson, F., Huq, S., Field, M., and Pollock, C.: Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor, J. Pharmacol. Exp. Ther. 289, 535-42, 1999
Human Human Renal cortex Trypsin: 0.1%
 Tissue Culture Grade Water McAteer, J, Kempson, S., and Evan, A: Culture of Human Renal Cortex Epithelial Cells, J. Tiss. Cult. Meth. 13, 143, 1991
Human Human Papillary duct Collagenase: 400 u/ml
 Eagle's MEM-HEPES buffer w/L-glutamine Trifillis, A. and Kahng, M.: Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells, In Vitro Cell. Dev. Biol. 26, 441, 1990
Human Human Mesangial Trypsin: 0.25%
 DMEM/Ham's F-12 Heieren, M., van der Woude, F., and Balfour Jr., H.: Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells, Proc. Natl. Acad. Sci. USA 85, 1642, 1988
Human Human, adult Tubular Collagenase: 250 u/ml
 PBS Yang, A., Gould-Kostka, J., and Oberley, T.: In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate, In Vitro Cell. Dev. Biol. 23 (1), 34, 1987
Human Human, adult, 14-66 years Tubular Collagenase: 100 u/ml
 Joklik's MEM Trifillis, A., Regec, A., and Trump, B.: Isolation, Culture, and Charaterization of Human Renal Tubular Cells, J. Urol. 133, 324, 1985
Human Human Malignant
Stromal		 Papain: 0.009%
 Sacks solution Hemstreet, G., Enoch, P., and Pretlow, T.: Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification, Cancer Res. 40, 1043, 1980
Human Human, 24 newborn and stillborn
(also rabbit)		 Renal Trypsin: 0.25%
 See reference De Oca, H., and Malinin, T.: Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys, J. Clin. Microbiol. 2, 243, 1975
Monkey Monkey Kidney Trypsin: 0.01% - 0.00001%
 Eagle's MEM Melnick, J., and Wallis, C.: Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells, Dev. Biol. 37, 77, 1976
Mouse Mouse, adult Cortex, proximal tubule Collagenase: 0.15%
 DMEM Syal Ashu, Schiavi Susan, Chakravarty Sumana, Dwarakanath Vangipuram, Quigley Raymond, Baum Michel: Fibroblast growth factor-23 increases mouse PGE2 production in vivo and in vitro, Am. J. Physiol. / Renal 290, F450-5, 2006
Mouse Mouse, 6 week Glomerular mesangial cells Collagenase Type 4: 0.1%
 RPMI 1640 Radeke HH, Janssen-Graalfs I, Sowa EN, Chouchakova N, Skokowa J, Loscher F, Schmidt RE, Heeringa P, Gessner JE.: Opposite regulation of type II and III receptors for immunoglobulin G in mouse glomerular mesangial cells and in the induction of anti-glomerular basement membrane (GBM) nephritis, J. Biol. Chem. 277(30), 27535, 2002
Mouse Mouse, 3-7 month Fibroblasts, mesangial, smooth muscle Trypsin: 0.25%
Collagenase: see reference
Soybean Trypsin Inhibitor: .05%
 DMEM Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation, Mol. Biol. Cell 10, 1569-79, 1999
Mouse Mouse Epithelial Collagenase Type 4: 1%
 DMEM /F-12 Taub, M: Renal Tubule Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley & Sons, Ltd., 14B:6.1, 1995
Mouse Mouse, naive SJL (H-2S) Proximal tubular epithelial Deoxyribonuclease I: 15 µg/ml
 RPMI 1640 Haverty, T., Kelly, C., Hines, W., Amenta, P., Watanabe, M., Harper, R., Kefalides, N., and Neilson, E.: Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis, J. Cell Biol. 107, 1359, 1988
Rabbit Rabbit, New Zealand White, female Nephron segments Collagenase Type 2: 0.05%
Deoxyribonuclease I: 50 u/ml
 Eagle's MEM Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments, Am. J. Physiol. 273, F650, 1997
Rabbit Rabbit, New Zealand white, male, 1.5 - 2.0 Kg Duct Soybean Trypsin Inhibitor: 0.025%
 Hank's Solution with calcium and HEPES Naray-Fejes-Toth, A., and Fejes-Toth, G.: Immunoelection and Culture of Cortical Collecting Duct Cells, J. Tiss. Cult. Meth. 13, 179, 1991
Rabbit Rabbit, New Zealand white, female, 2-3 Kg Renal proximal tubules Deoxyribonuclease I: 70 u/ml
 Modified DME-F12 Rodeheaver, D., Aleo, M., and Schnellmann, R.: Differences in Enzymatic and Mechanical Isolated Rabbit Renal Proximal Tubules: Comparison in Long-Term Incubation, In Vitro Cell. Dev. Biol. 26, 898, 1990
Rabbit Rabbit
(also rat, SD and Wistar, 150-225 g, hamster, bovine)		 Papillary duct Hyaluronidase: 0.2%
 Kreb's buffer
HEPES buffered saline		 Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am. J. Physiol. 255, F1160, 1988
Rabbit Rabbit, New Zealand, white, 2-3 kg MTALH cells
RCCT cells		 Collagenase: 0.1%
 DMEM Allen, M., Nakao, A., Sonnenburg, W., Burnatowska-Hledin, M., Speilman, W., and Smith, W.: Immunodissection of Cortical and Medullary Thick Ascending Limb Cells From Rabbit Kidney, Am. J. Physiol. 255, F704, 1988
Rabbit Rabbit, New Zealand, white Collecting tubule Trypsin: 0.05%
 Kreb's Ringer buffer Grenier, F., and Smith, W.: Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae, Prostaglandins 16, 759, 1978
Rabbit Rabbit, New Zealand, white, female, 2.5 Kg Papillary collecting duct Collagenase: 0.2%
 (see reference) Dworzack, D., and Grantham, J.: Preparation of Renal Papillary Collecting Duct Cells for Study, Nat. Neurosci. 8, 191, 1975
Rat Rat, SD, male, 200-250g Medullary thick ascending limb Collagenase: 0.1%
 HEPES-saline Silva, G., Beierwaltes, W., and Garvin, J.: Extracellular ATP stimulates NO production in rat thick ascending limb, Hypertension 47, 563-7, 2006
Rat Rat, Wistar, 300g Renal proximal tubules Collagenase Type 2: 0.2%
 DMEM/F12 Deng Aihua, Miracle Cynthia M, Lortie Mark, Satriano Joseph, Gabbai Francis B, Munger Karen A, Thomson Scott C, Blantz Roland C: Kidney oxygen consumption, carbonic anhydrase, and proton secretion, Am. J. Physiol. / Renal 290, F1009-15, 2006
Rat Rat Cortical tubule cells Collagenase: 0.6%
 DMEM Arystarkhova, E., Wetzel R., and Sweadner K.: Distribution and Oligomeric Association of Splice Forms of Na(+)-K(+)-ATPase Regulatory Gamma-subunit in Rat Kidney, Am. J. Physiol. / Renal 282(3), F393, 2002
Rat Rat, SD, male 275-300 g Glomerular mesangial cells Collagenase Type 1: 250 u/ml
 DMEM Amiri Farhad, Shaw Sean, Wang Xiaodan, Tang Jie, Waller Jennifer L, Eaton Douglas C, Marrero Mario B: Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose, Kidney Int. Suppl. 61, 1605-16, 2002
Rat Rat, SD, 1 week Cortical collection duct Collagenase Type 2: 0.15%
 DMEM/Ham's F12 Valencia L., Bidet M., Martial S., Sanchez E., Melendez E., Tauc M., Poujeol C., Martin D., Namorado M., Reyes J., and Poujeol P.: Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture, Am. J. Physiol. Cell Physiol. 280(5), C1193, 2001
Rat Rat, SD, 250-300 g Microvessels Collagenase Type 2: 0.1%
 PSS Li, N., Teggatz, E., Li, P., Allaire, R., and Zou, A.: Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels, Microvasc. Res. 60, 149, 2000
Rat Rat, SD, 250-300 g Renal Collagenase Type 2: 0.2%
 see reference Mattson D., and Wu F.: Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature, Hypertension 35, 337, 2000
Rat Rat, SD, male, 5-6 week Renal tubules Collagenase: 0.1%
 see reference Miyata, N., Park, F., Li, X., and Cowley, A.: Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney, Am. J. Physiol. 277, F437, 1999
Rat Rat, SD, 225-250 g Glomerular mesangial cells Collagenase Type 1: 250 u/ml
 DMEM Amiri F, Garcia R: Regulation of angiotensin II receptors and PKC isoforms by glucose in rat mesangial cells, Am. J. Physiol. 276, F691-9, 1999
Rat Rat, SD, male Proximal tubule suspensions Collagenase Type 4: 0.1%
Pronase E: 2 u/ml
 HEPES buffer Eitle E, Hiranyachattada S, Wang H, Harris PJ: Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney, Am. J. Physiol. 274, C1075-80, 1998
Rat Rat, SD, male Nephron segments Collagenase Type 2: 0.05%
Deoxyribonuclease I: 50 u/ml
 Eagle's MEM Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments, Am. J. Physiol. 273, F650, 1997
Rat Rat, 12-weeks-old Renal Collagenase: 0.1%
 DMEM Ishikawa, S., Kusaka, I., Higashiyama, M., Nagasaka, S., Saito, T., Honda, K., and Saito, T.: Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein, Nat. Neurosci. 50, 1506, 1996
Rat Rat, male, 150-200 g Epithelial
Proximal tubule		 Protease: 0.1%
 HBSS/CMF Elliget, K., and Trump, B.: Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury, In Vitro Cell. Dev. Biol. 27, 739, 1991
Rat Rat, adult Inner medullary collecting duct
Papillae		 Collagenase Type 2: 0.1%
 PBS Brion, L., Schwartz, J., Lachman, H., Zavilowitz, B., and Schwartz, G.: Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells, Am. J. Physiol. 257, F486, 1989
Rat Rat, Wistar, female, 100-150 g Glomerular mesangial Collagenase: 0.025%
 RPMI 1640 Wang, J., Kester, M., and Dunn, M.: The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells, Biochim. Biophys. Acta 969, 217, 1988
Rat Rat, Wistar, male, 150 - 200 g Renal target Collagenase: 1.0% (also 0.7%)
 Eagle's MEM Barlet-Bas, C., Khadouri, C., Marsey, S., and Doucet, A.: Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine, Proc. Natl. Acad. Sci. USA 85, 1707, 1988
Rat Rat, Wistar, male, adult Proximal tubules Collagenase: 0.2%
 Krebs-Henseleit buffer Gesek, F., Wolff, D., and Strandhov, J.: Improved Separation Method for Rat Proximal and Distal Renal Tubules, Am. J. Physiol. 253, F358, 1987
Rat Rat,Wistar, 300-400 gm Proximal tubules Collagenase: 0.15 %
 Krebs Henseleit solution Vinay, P., Gougoux, A., and Lemieux, G.: Isolation of a Pure Suspension of Rat Proximal Tubules, Am. J. Physiol. 241, F403, 1981
Rat Rat Fibroblasts
Kidney		 Trypsin: up to 0.25%
 Dulbecco-Vogt MEM Wallach, D., Anderson, W., and Pastan, I.: Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin, J. Biol. Chem. 253, 24, 1978