Heart - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine
(also rat)		 Heart Adrenal chromaffin Paraneurons Trypsin: 0.06%
 25mM HEPES buffered Locke's solution, CMF Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons , In Vitro Cell Dev Biol 26, 335, 1990
Canine Dog, mongrel, adult Myocytes Hyaluronidase: 0.1%
 Joklik's MEM Ca2+-Tolerant Adult Canine Myocytes: Preparation and Response to Anoxia/Acidosis , Am J Physiol 243, H448, 1982
Chicken Chicken embryos, 9-11 day incubated Cardiomyocytes Trypsin: 0.25%
 PBS Three-Dimensional Reconstitution of Embryonic Cardiomyocytes in a Collagen Matrix: A New Heart Muscle Model System , FASEB J 11, 683, 1997
Chicken Chick, embryos, 10-11 day Cardiomyocytes Trypsin: 0.17%
 HBSS, CMF Studies on Cardiac Myofibrillogenesis With Antibodies to Titin, Actin, Tropomyosin, and Myosin , J Cell Biol 107, 1075, 1988
Chicken Chick, 10 day Ventricular Trypsin: 0.025%
 HBSS, CMF Control of Cytosolic Calcium Activity During Low Sodium Exposure in Cultured Chick Heart Cells , Circ Res 61, 29, 1987
Chicken Chick, embryo, 11 day Cardiomyocytes Trypsin: 0.05%
 HBSS, CMF Effect of Sodium-Potassium Pump Inhibition and Low Sodium on Membrane Potential in Cultured Embryonic Chick Heart Cells , J Physiol 387, 549, 1987
Chicken Chick, embryonic, 11-12-day-old Myocytes Trypsin: 0.025%
 CMF solution Calcium Elevation in Cultured Heart Cells: Its Role in Cell Injury , Am J Physiol Cell Physiol 245 (14), C316, 1983
Chicken Chick, 8 day embryo
(also rat, neonate and mouse)		 Myocytes Trypsin NF 1:250: 0.25%
 Rinaldini's buffer solution, CF Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells , Exp Cell Res 53, 1, 1968
Chicken Hen, White Leghorn Heart Trypsin: 0.025%
 Medium 199 Regulation of Spontaneous Activity and Growth of Embryonic Chick Heart Cells in Tissue Culture , Dev Biol 16, 216, 1967
Chicken Chick embryos, 5 day Heart Liver Trypsin: 3.0%
 Tyrode's solution, CMF "ECM": Its Nature, Origin, And Function in Cell Aggregation , Exp Cell Res 30, 257, 1963
Feline Cat, mongrel, adult, 1.8-2.8 Kg Myocytes Ventricular Collagenase Type 2: 0.12%
 Kreb's Henseleit, CF Isolation and Morphology of Calcium-Tolerant Feline Ventricular Myocytes , Am J Physiol 245, H891, 1983
Fish Octopus, E. cirhosa, 260-352 g systemic heart cardiomyocytes Collagenase: 0.025%
Trypsin: 0.02%
 see reference Ca(2+) Uptake in the Sarcoplasmic Reticulum from the Systemic Heart of Octopod Cephalopods , J Exp Biol 202, 2531, 1999
Frog Frog (Rana esculenta) Myocytes Trypsin: 0.04%
 CF Ringer High Yield Preparation of Calcium-Tolerant Myocytes From Frog Ventricles, Biol Cell 54, 164, 1985
Guinea-Pig guinea pig, adult cardiomyocytes Collagenase Type 2: 100 u/ml
 M-199 L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes. , Physiol Res 53(4), 369, 2004
Guinea-Pig guinea pig, female, 300-350g coronary endothelial Collagenase Type 2: 0.1%
 see reference Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium. , Am J Physiol Heart Circ Physiol 281, H1657-66, 2001
Guinea-Pig Guinea pig, 250-450 g Endothelial Collagenase Type 2: 0.15%
 Perfusing solution
(see reference)		 Separation of Cardiomyocytes and Coronary Endothelial Cells for Cell-Specific RT-PCR , Am J Physiol 277, H413, 1999
Guinea-Pig Guinea-pig, male, 450-600 g Myocytes Hyaluronidase: 0.02%
 Krebs Henseleit bicarbonate buffer Isolation and Enrichment of Ca2+-Tolerant Myocytes for Biochemical Experiments from Guinea-Pig Heart , Life Sci 44, 1231, 1989
Guinea-Pig Guinea-pig Cardiomyocytes Collagenase: 0.04%
 Tyrode solution, CF The Mg2+ Block and Intrinsic Gating Underlying Inward Rectification of the K+ Current in Guinea-Pig Cardiac Myocytes , J Physiol 419, 297, 1989
Guinea-Pig Guinea-pig Myocytes Hyaluronidase: 0.10%
 Bicarbonate buffer, CF Relaxation of Isolated Ventricular Cardiomyocytes by a Voltage-Dependent , Science 241, 823, 1988
Human Human (n=16, age: 60 &plusmin;3 years) Myocardial Hyaluronidase: 0.05%
 Medium 199 Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response. , J Cardiovasc Pharmacol 7 (4), 235, 2002
Human Human Heart Myocytes Collagenase Type 2: 200 u/ml
 Tyrode's solution Hyperpolarization-Activated Inward Current in Ventricular Myocytes From Normal and Failing Human Hearts , Circulation 97, 55, 1998
Human Human, fetal, 14.5 week gestation Myocytes Hyaluronidase: 0.1%
 HBSS with Calcium Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes , In Vitro Cell Dev Biol 28, 109, 1992
Human Human, both sexes, 18-81 years Myocardial Atrial Collagenase: 0.14%
 HBSS, CMF A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor , In Vitro Cell Dev Biol 27, 914, 1991
Human Human, adult Myocytes Trypsin: 0.25%
 Joklik's MEM Biology of Isolated Adult Cardiac Myocytes, Isolation and Culture of Human Adult Cardiac Myocytes,(Clark,Decker,Borg,eds), Elsevier Science Publishing Co., Inc., , 1988
Human Human Thoracic aorta Collagenase: 0.2%
 Phosphate buffer w/NaCl The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase, Acta Pathol Microbiol Scand 57, 15, 1963
Mouse mouse, neonatal cardiomyoctes Collagenase Type 1: 150 u/ml
Trypsin: 0.01%
 M199 ClC-3-independent sensitivity of apoptosis to Cl- channel blockers in mouse cardiomyocytes , Cell Physiol Biochem 15, 263, 2005
Mouse mouse, 1 day cardiomyoctes NCIS kit:
EBSS Reduced Apaf-1 levels in cardiomyocytes engage strict regulation of apoptosis by endogenous XIAP , J Cell Biol 171, 925, 2005
Mouse mouse smooth muscle cells Collagenase Type 1: 0.1%
Elastase: 0.0125%
 DMEM Genetically determined resistance to collagenase action augments interstitial collagen accumulation in atherosclerotic plaques. , Circulation 110, 1953-9, 2004
Mouse mouse, 6-8 week vascular smooth muscle Papain: 10 u/ml
Elastase: .005%
Collagenase: 0.05%
Deoxyribonuclease I: 1000 u/ml
PDS kit: with modifications
 EBSS Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells , Hum Mol Genet 12(15), 1875, 2003
Mouse mouse cardiomyocytes Collagenase Type 2: 0.2%
Pancreatin: 0.06%
 DMEM/F-12 Conditional Expression of SV40 T-antigen in Mouse Cardiomyocytes Facilitates an Inducible Switch from Proliferation to Differentiation , J Biol Chem 278(18), 15927, 2003
Mouse mouse, neonatal 2-4 day atrial myocytes NCIS kit: with modifications see reference
 L-15 Contribution of the Kir3.1 Subunit to the Muscarinic-gated Atrial Potassium Channel IKACh. , J Biol Chem 277, 48282, 2002
Mouse mouse, neonatal and adult cardiomyocytes Collagenase Type 2: 0.03-0.2%
 Joklik's MEM Protein Kinase C Isoform Expression and Activity in the Mouse Heart , Am J Physiol Heart Circ Physiol 281(5), H2062, 2001
Mouse Mouse, NTg and Tg, 8 - 12 week Myocytes Collagenase Type 2: 150 u/ml
 Joklik's MEM Proinflammatory Consequences of Transgenic Fas Ligand Expression in the Heart , J Clin Invest 105 (9), 1199, 2000
Mouse mouse, 1-6 day cardiomyocytes Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.002%
 PBS Selection of viable cardiomyocytes for cell transplantation using three-dimensional tissue culture. , Transplantation 70, 1310, 2000
Mouse mouse, 2-4 mo ventricular myocytes Collagenase Type 2: 0.05%
Collagenase Type 4: 0.05%
Protease XIV: 0.002%
 MEM Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology. , Am J Physiol Heart Circ Physiol 279, H429-36, 2000
Mouse mouse, 1-3 day cardiomyocytes Trypsin: 0.25%
 FBS-MEM Inhibition of Doxorubicin Toxicity in Cultured Neonatal Mouse Cardiomyocytes with Elevated Metallothionein Levels , J Pharmacol Exp Ther 288(3), 938, 1999
Mouse mouse, 1 day cardiomyocytes NCIS kit: with modifications see reference
 L-15 Role of Gelsolin in the Actin Filament Regulation of Cardiac L-type Calcium Channels , Am J Physiol 277, C1277, 1999
Mouse mouse, 3-7 month fibroblasts, mesangial, smooth muscle Trypsin: 0.25%
Collagenase: see reference
Soybean Trypsin Inhibitor: .05%
 DMEM Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation. , Mol Biol Cell 10, 1569-79, 1999
Mouse Mouse (BALB/c), adult Myocytes Collagenase Type 2: 0.04%
 Tyrode's solution Fas (CD95/Apo-1)-Mediated Damage to Ventricular Myocytes Induced by Cytotoxic T Lymphocytes from Perforin-Deficient Mice , Circ Res 82, 438, 1998
Mouse mouse, 1 day cardiomyocytes NCIS kit: with modifications see reference
 L-15 Cardiac Gsalpha Overexpression Enhances L-type Calcium Channels Through an Adenylyl Cyclase Independent Pathway , Proc Natl Acad Sci U S A 95, 9669, 1998
Mouse Mouse, P0-P1 Cardiomyocytes Collagenase: 0.045%
 DMEM, Medium 199 w/ 10% horse serum and 5% fetal calf serum MLP-Deficient Mice Exhibit a Disruption of Cardiac Cytoarchitectural Organization, Dilated Cadiomyopathy, and Heart Failure , Cell 88, 393, 1997
Mouse mouse, 6-9 or 21-41 day ventricular and atrial myocytes Collagenase Type 2: 95 u/ml
Hyaluronidase: 172.5 u/ml
Trypsin: 0.002%
Deoxyribonuclease I: 60u/ml
 DMEM/Tyrodes G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart , Proc Natl Acad Sci U S A 94(5), 1727, 1997
Mouse Mouse Cardiomyocyte Collagenase: 0.17 %
 PBS Cardiomyocyte DNA Synthesis and Binucleation During Murine Development , Am J Physiol 271, H2183, 1996
Mouse Mouse embryos Myocytes Collagenase Type 1: 0.1%
 DMEM Cardiomyocyte-Like Cells Differentiated In Vitro From Embryonic Carcinoma Cells P19 are Characterized by Functional Expression of Adrenoceptors and Ca2+ Channels , In Vitro Cell Dev Biol 30A, 425, 1994
Mouse Mouse
(also rat, neonate and chick, 8 day embryo)		 Myocytes Trypsin NF 1:250: 0.25%
 Rinaldini's buffer solution, CF Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells , Exp Cell Res 53, 1, 1968
Porcine Porcine Coronary vascular smooth muscle (PCVSMCs) Trypsin: 0.037%
 HEPES Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells , Circulation 99, 1485, 1999
Porcine Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise Coronary smooth muscle Trypsin: 0.1%
 Krebs bicarbonate solution, iced Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle , Am J Physiol 275 (44), H2159, 1998
Rabbit Rabbit, New Zealand White, 2-3 kg Myocytes Collagenase Type 2: 0.1%
 HEPES Generation of Intracellular pH Gradients in Single Cardiac Myocytes with a Microperfusion System , Am J Physiol 278, H1371, 2000
Rabbit Rabbit, New Zealand, white, either sex Myocytes Collagenase Type 2: 0.05%
 EGTA-KB Colocalization of Dihydropyridine and Ryanodine Receptors in Neonate Rabbit Heart Using Confocal Microscopy , Am J Physiol Heart Circ Physiol 279, H202, 2000
Rabbit rabbit, adult 1.5-2.5 kg tricuspid valve cells Collagenase: 0.8 mg/ml
 HEPES Tyrode solution Electrophysiology of single heart cells from the rabbit tricuspid valve. , J Physiol 425, 145, 1990
Rabbit Rabbit, New Zealand, white, male, 2-3 kg Cardiomyocytes Hyaluronidase: 0.5%
 Eagle's MEM Compartments of Cyclic AMP and Protein Kinase in Mammalian Cardiomyocytes , J Biol Chem 258 (17), 10233, 1983
Rabbit Rabbit, adult, 2 kg Myocytes Hyaluronidase: 0.007%
 Kreb's Ringer Preparation and Some Properties of Isolated Beating Myocytes from Adult Rabbit Heart , J Mol Cell Cardiol 9, 777, 1977
Rat Rat, SD, 300-350 g cardiomyocytes Collagenase Type 2: 0.05%
Hyaluronidase: 0.02%
 DMEM An essential role for complement C5a in the pathogenesis of septic cardiac dysfunction. , J Exp Med 203, 53-61, 2006
Rat rat, 1-2 day cardiomyocytes NCIS kit: per instructions
 DMEM/F-12 Estrogen Inhibits Cardiomyocyte Hypertrophy in vitro. Antagonism of Calcineurin-related Hypertrophy through Induction of MCIP1. , J Biol Chem 280, 26339, 2005
Rat Rat, SD, 2-4 day cardiomyocytes, fibroblasts Trypsin: 0.1%
Collagenase: 0.1%
 medium 199 Functional Cardiac Cell Constructs on Cellulose-based Scaffolding , Biomaterials 25(26), 5753, 2004
Rat rat, SD, 1-2 day cardiomyocytes NCIS kit: per instructions
 L-15 Adult-derived Liver Stem Cells Acquire a Cardiomyocyte Structural and Functional Phenotype ex vivo. , Am J Pathol 165, 135, 2004
Rat rat,1-2 day cardiomyocytes NCIS kit: per instructions
 DMEM Intrinsic cardiac catecholamines help maintain beating activity in neonatal rat cardiomyocyte cultures. , Pediatr Res 56, 411, 2004
Rat rat, 1 day cardiomyocytes NCIS kit: per instructions
 F-10 Down-regulation of the M6P/IGF-II Receptor Increases Cell Proliferation and Reduces Apoptosis in Neonatal Rat Cardiac Myocytes , BMC Cell Biol 5, 15, 2004
Rat Rat, neonatal cardiac myocytes Collagenase Type 2: 0.5%
 HBSS Vgl-4, a novel member of the vestigial-like family of transcription cofactors, regulates alpha1-adrenergic activation of gene expression in cardiac myocytes. , J Biol Chem 279, 30800-6, 2004
Rat Rat, male, 250-300 g cardiomyocytes Collagenase Type 1: 0.03%
Protease: 0.01%
 HBSS Adult rat cardiomyocytes exhibit capacitative calcium entry. , Am J Physiol Heart Circ Physiol 286, H1124-32, 2004
Rat Rat, fetal or 3-day cardiomyocytes Collagenase Type 2: 0.1%
 HEPES Critical role of cyclin D1 nuclear import in cardiomyocyte proliferation. , Circ Res 92, e12-9, 2003
Rat rat, adult, 220-280g cardiomyocytes Collagenase Type 2: 178 u/ml
Hyaluronidase: 0.01%
 Krebs-Henseleit calcium free Cardioprotective action of CRF peptide urocortin against simulated ischemia in adult rat cardiomyocytes. , Am J Physiol Heart Circ Physiol 284, H330-6, 2003
Rat rat, 50 g ventricular myocytes Collagenase: 223 u/ml
Hyaluronidase: 0.01%
Trypsin: 0.002%
 Medium 199 Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy. , Biol Proced Online 5, 1, 2003
Rat rat, neonatal cardiomyocytes NCIS kit: per instructions
 L-15 Long-term survival of xenografted neonatal cardiomyocytes by adenovirus-mediated CTLA4-Ig expression and CD40 blockade. , Circulation 108, 1760, 2003
Rat Rat, SD, pregnant female Peritoneal mast Hyaluronidase: 100 u/ml
 DMEM (see reference) Effects of mast cells on the behavior of isolated heart fibroblasts: modulation of collagen remodeling and gene expression. , J Cell Physiol 191, 51, 2002
Rat rat, neonatal, adult, 17 day ventricular cardiomyocytes Collagenase Type 2: 0.7-1%
 DMEM The 21-day postnatal rat ventricular cardiac muscle cell in culture as an experimental model to study adult cardiomyocyte gene expression. , Mol Cell Biochem 229, 51, 2002
Rat rat, 1 day cardiomyocytes NCIS kit: per instructions
 L-15 Enhancement of nitric oxide production by methylecgonidine in cultured neonatal rat cardiomyocytes , Br J Pharmacol 135, 188, 2002
Rat rat, female, 250-300 g ventricular myocytes Collagenase Type 2: 0.1%
 Ringer solution Antiadrenergic effects of adenosine A(1) receptor-mediated protein phosphatase 2a activation in the heart. , Am J Physiol Heart Circ Physiol 283, H1314-21, 2002
Rat Rat, Wistar, 1 day cardiomyocytes Collagenase Type 2: 80u/ml
 M199 Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces. , Circ Res 90, e40, 2002
Rat Rat, male, 6-12 months old Cardiac myocytes Protease: 0.55 u/ml
 Tyrode's solution Hepatocyte Growth Factor Protects Cardiac Myocytes Against Oxidative Stress-Induced Apoptosis , Free Radic Biol Med 31 (7), 902, 2001
Rat Rat, Harlan SD, pup Cardiomyocytes Trypsin:
DMEM/Ham's F-12 The Tumor Suppressor Gene PTEN Can Regulate Cardiac Hypertrophy and Survival , J Biol Chem 276, 35786, 2001
Rat rat, SD, newborn cardiac myocytes NCIS kit: per instructions
 L-15 Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro. , Biol Res 34(3-4), 195, 2001
Rat rat, adult ventricular myocytes Collagenase: 223 u/ml
Hyaluronidase: 0.01%
Trypsin: 0.002%
 Tyrode solution Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging. , Biol Proced Online 3, 43, 2001
Rat Rat, SD, male cardiomyocytes Collagenase Type 2: 140 u/ml
 Krebs-Henseleit Bioenergetics in cardiac hypertrophy: mitochondrial respiration as a pathological target of NO* , Am J Physiol/Heart 281, H2261-H2269, 2001
Rat rat, neonatal cardiomyocytes NCIS kit: per instructions
 DMEM/M199 Hypoxia induces the expression of the pro-apoptotic gene BNIP3. , Cell Death Differ Vol. 8,, , 367-76, 2001
Rat Rat, Wistar, female ventricular myocytes Collagenase Type 2: 0.1%
 Ringer solution Effects of Kappa-opioid receptor activation of myocardium , Am J Physiol Heart Circ Physiol 281, H669, 2001
Rat Rat, newborn Atrial Trypsin: 0.06%
 DMEM Cytoplasmic Unsaturated Free Fatty Acids Inhibit ATP-Dependent Gating of the G Protein-gated K+ Channel , J Gen Physiol 115, 287, 2000
Rat rat, 1-2 day cardiomyocytes Collagenase Type 1: 84 u/ml
Pancreatin: 0.05%
 M199 medium The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice. , EMBO J 19(23), 6341, 2000
Rat rat, Winstar, 1-2 day ventricular myocytes Collagenase Type 2: 0.08%
 DMEM Effect of neutral endopeptidase inhibitor on endogenous atrial natriuretic peptide as a paracrine factor in cultured cardiac fibroblasts. , Br J Pharmacol 131(6), 1204, 2000
Rat rat, SD, adult female heart microvascular cells CLSPA: 250u/ml
Papain: 5 u/ml
Elastase: 0.8 u/ml
 L-15 Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3). , Am J Physiol/Endo 278(2), E293, 2000
Rat rat, SD, male, 250-300g cardiomyocytes Collagenase Type 2: 0.06%
 M199 Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment. , Am J Physiol Heart Circ Physiol 278(4), H1091, 2000
Rat rat, wistar, 2 day cardiomyocytes NCIS kit: per instructions
 DMEM-F12 CD14-independent activation of cardiomyocyte signal transduction by bacterial endotoxin. , Am J Physiol Heart Circ Physiol 279, H619, 2000
Rat rat, neonatal cardiomyocytes NCIS kit: per instructions
 L-15 Beating rate of isolated neonatal cardiomyocytes is regulated by the stable microtubule subset , Am J Physiol Heart Circ Physiol 278, H1653, 2000
Rat Rat, Lewis, adult male, 300-350-g Aorta, smooth muscle Trypsin: 0.025%
 Meduim 199 with 20% FBS medium Engineered Smooth Muscle Tissues: Regulating Cell Phenotype with the Scaffold , Exp Cell Res 251, 318, 1999
Rat Rat, 10-15 newborn, 1-2 days old Atrial Trypsin: 0.03%
 HBSS or EBSS GBy Binding Increases the Open Time of IKACh: Kinetic Evidence for Multiple GBy Binding Sites , Biophys J 76, 246, 1999
Rat rat, 1-3 day neonatal rat cardiomyocytes Collagenase Type 2: 80u/ml
Pancreatin: 0.06%
 DMEM/F12 Oxidative damage of cardiomyocytes is limited by extracellular regulated kinases 1/2-mediated induction of cyclooxygenase-2. , J Biol Chem 274, 5038, 1999
Rat rat, 1-2 day cardiomyocytes Pancreatin: 0.06%
Collagenase Type 2: 95 u/ml
 DMEM Survival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat hearts , Circulation 100(2), 193, 1999
Rat rat, adult and neonatal cardioomyocytes Collagenase Type 4: 0.12%-adult
Collagenase Type 2: 0.05%-neonatal
 DMEM/medium 199 A spliced variant of AE1 gene encodes a truncated form of Band 3 in heart: the predominant anion exchanger in ventricular myocytes. , J Cell Sci 112, 1519, 1999
Rat rat, Wistar, male cardiomyocytes Collagenase Type 2: 228 u/ml
 Joklik MEM Localization of lipoprotein lipase in the diabetic heart: regulation by acute changes in insulin. , Arterioscler Thromb Vasc Biol 19(6), 1526, 1999
Rat rat, 1-2 day cardiomyocytes NCIS kit: per instructions
 RPMI Adenoviral gene transfer of activated phosphatidylinositol 3'-kinase and Akt inhibits apoptosis of hypoxic cardiomyocytes in vitro. , Circulation 100, 2373-9, 1999
Rat Rat Thoracic aorta, smooth muscle Elastase: 0.02%
 (see reference) Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression , Circ Res 82, 566, 1998
Rat rat, SD, female, 200g ventricular cardiac myocytes Collagenase Type 2: 0.5%
Hyaluronidase: 0.2%
 Krebs-Henseleit buffer Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function. , Proc Natl Acad Sci U S A 94, 5444-9, 1997
Rat Rat, Wistar, 1 day cardiomyoctes Collagenase Type 2: 80 u/ml
 HBSS Cardiac inducible nitric oxide synthase negatively modulates myocardial function in cultured rat myocytes. , Am J Physiol 272, H35-47, 1997
Rat Rat, neonatal, 5-day Cardiomyocytes Trypsin: 0.05%
 DMEM Cardiogel: A Biosynthetic Extracellular Matrix for Cardiomyocyte Culture , In Vitro Cell Dev Biol Anim 32, 478, 1996
Rat rat, SD, 250-300 g cardiomyocytes Collagenase Type 2: 0.05%
 Joklik's Molecular and functional identification of m1 muscarinic acetylcholine receptors in rat ventricular myocytes , Circ Res 79(1), 86, 1996
Rat Rat, SD, male, 200-250 g Myocytes Collagenase: 0.07%
 Joklik's MEM with HEPES Effect of Exercise Training on Intracellular Free Ca2+ Transients in Ventricular Myocytes of Rats , J Appl Physiol 73, 1441, 1992
Rat Rat, SD, male, 200-250 g Myocytes Ventricles Collagenase: 0.07%
 Joklik's MEM Effect of Exercise Training on Intracellular Free Ca2+ Transients in Ventricular Myocytes of Rats , Am J Physiol 73, 1141, 1992
Rat Rat, SD, female, 60-90 days Myocardial Collagenase Type 2: 0.1%
 Joklik's MEM, Kreb's-Henseleit buffer, CF A Primary Culture System of Adult Rat Heart Cells for the Study of Toxicologic Agents , In Vitro Cell Dev Biol 27, 921, 1991
Rat Rat, SD, adult, female, 180-220 g Cardiomyocytes Collagenase: 0.11%
 (see reference) Highly Insulin-Responsive Isolated Rat Heart Muscle Cells Yielded By a Modified Isolation Method , Life Sci 49, 1679, 1991
Rat Rat, neonatal, 1-4 days Cardiomyocytes Collagenase Type 2: 0.12%
 Krebs-Henseleit Buffer, CF Effects of Norepinephrine on Neonatal Rat Cardiocyte Growth and Differentiation , In Vitro Cell Dev Biol 26, 229, 1990
Rat Rat
(also bovine)		 Heart Adrenal chromaffin Paraneurons Trypsin: 0.06%
 25mM HEPES buffered Locke's solution, CMF Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons , In Vitro Cell Dev Biol 26, 335, 1990
Rat Rat, adult, female Myocytes Collagenase Type 2: 0.05%
 Joklik's MEM Adult Cardiac Muscle Cells in Long-Term Serum-Free Culture:Myofibrillar Organization and Expression of Myosin Heavy Chain Isoforms , In Vitro Cell Dev Biol 26, 464, 1990
Rat Rats, AW, late-gestation pregnant females Ventricular Cardiomyocytes Collagenase Type 2: 0.05 - 0.08%
 See reference , Technique 2 (6), 279, 1990
Rat Rat,SD, albino, 10-14 weeks Myocytes Collagenase Type 1: 100 u/ml and 150 u/ml
 Krebs Ringer bicarbonate buffer Carbamoylcholine-Induced Accumulation of Inositol Mono-,Bis-,Tris-, And Tetrakisphosphates In Isolated Cardiac Myocytes From Adult Rats , Biochim Biophys Acta 1010, 100, 1989
Rat Rat, SD, adult, female, 175 - 225 g Myocytes Collagenase: 0.1%
 Joklik's medium Differentiation of Adult Rat Cardiac Myocytes in Cell Culture , Circ Res 64, 493, 1989
Rat Rat, SD, male, 250 - 350 g Myocytes, ventricular Collagenase Type 1: 90 and 100 u/ml
 Joklik's solution with and without calcium Isolation of Calcium-Tolerant Atrial And Ventricular Myocytes From Adult Rat Heart , Methods Enzymol 173, 662, 1989
Rat Rat, SD, 2-4 day old Cardiomyocytes Fibroblasts Trypsin: 0.1%
 HBSS Comparative Toxicity of Allylamine and Acrolein in Cultured Myocytes and Fibroblasts from Neonatal Rat Heart , Toxicology 56, 107, 1989
Rat Rat, SD, male Aortic smooth muscle Trypsin: 0.05%
 DMEM Regulation of Intracellular Ca2+ Levels in Cultured Vascular Smooth Muscle Cells , J Biol Chem 264 (2), 1146, 1989
Rat Rat, adult, 150-200 g Myocytes, atria Collagenase Type 1: 100 u/ml
 Kreb's Ringer bicarbonate - HEPES buffer A Study of Adult Rat Atrial Myocyte Attachment to Extricellular Matrix Components and Long Term Culture, Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, Eds), Elsevier Science Publishing Co. Inc., , 1988
Rat Rat, SD Myocytes Collagenase Type 2: 0.1%
 Kreb's Ringer w/ calcium Alpha1-Adrenergic Receptor Signal Transduction in the Adult Rat Cardiac Myocyte, Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), , 298, 1988
Rat Rat, SD, male, 250-350 g Myocytes, heart Hyaluronidase: 0.03%
 Bicarbonate-buffered medium Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes, Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), , 370, 1988
Rat Rat, Wistar, 2-4 day neonates Myoblast, cardiac Trypsin: 0.1%
 Standardized Medium
(see reference)		 Primary Rat Cardiac Cell Culture: Diet of the Mother Rats as a Determinant Parameter of Cardiomyoblast Production from Neonates , Biol Cell 57, 89, 1986
Rat Rat, SD, timid-pregnant Myocytes Trypsin: 2.4 u/ml
 DMEM Control of Enzyme Activity Levels by Serum and Hydrocortisone in Neonatal Rat Heart Cells Cultured in Serum-Free Medium , J Cell Physiol 120, 126, 1984
Rat Rat, SD, female, 12 weeks Myocytes Collagenase Type 1: 0.06%
 Krebs Ringer bicarbonate buffer Culturing of Calcium Stable Adult Cardiac Myocytes , J Mol Cell Cardiol 14, 397, 1982
Rat Rat, male/female, 200-250 g Muscle Hyaluronidase: 0.1%
 Krebs Ringer phosphate buffer, CMF Adult Mammalian Cardiac Muscle Cells in Culture , Tissue Cell 13, 515, 1981
Rat Rat, female, adult, 200 - 300 g Myocytes Collagenase Type 2: 0.1%
 Joklik's MEM Culture of The Terminally Differentiated Adult Cardiac Muscle Cell: A Light and Scanning Electron Microscope Study , Dev Biol 80, 466, 1980
Rat Rat, SD, adult, male, 200-250 g Myocytes Hyaluronidase: 0.10%
 Joklik MEM, CF Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation , Life Sci 27, 815, 1980
Rat Rat, SD, female, 12 wks, 250 g Myocytes Ventricular myocardium Collagenase Type 1: 0.20%
 Kreb's Ringer bicarbonate buffer Electrical Properties of Individual Cells Isolated From Adult Rat Ventricular Myocardium , J Physiol 302, 131, 1980
Rat Rat, 10 wk Myocytes Collagenase Type 1: 0.1%
 Saline Regional Myocyte Size in Normotensive and Spontaneously Hypertensive Rats , Hypertension 1 (4), 378, 1979
Rat Rat, male, 200-300 g Myocytes Hyaluronidase: 82 u/ml
 Kreb's Henseleit buffer Isolation and Characterization of Adult Rat Heart Cells , Arch Biochem Biophys 179, 545, 1977
Rat Rat, female, albino, 200-250 g Myocytes Hyaluronidase: 0.1%
 Kreb's Ringers phosphate Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability , Tissue Cell 9 (3), 419, 1977
Rat Rat, adult Myocytes Collagenase: 0.1%
 Perfusing solution Cyclic Amp Formation and Morphology of Myocardial Cells Isolated from Adult Heart: Effect of Ca2+ and Mg2+ , Biochim Biophys Acta 421, 411, 1976
Rat Rat, SD, albino, male, 180-230 g Heart ventricles, beating Collagenase: 0.05-0.2%
 Phosphate buffer Dispersion and Isolation of Beating Cells from Adult Rat Heart , Anal Biochem 61, 32, 1974
Rat Rat, Hebrew Un strain, 3 & 17 months, 150-210 g Myocytes Trypsin: 0.05%
 Versene buffer Lipoprotein Uptake and Metabolism by Rat Aortic Smooth Muscle Cells in Tissue Culture , Circ Res 35, 136, 1974
Rat Rat, 2-5 day Myocytes Trypsin: 0.01%
 Saline A Pancreatic Enzyme Requirements for the Dissociation of Rat Hearts for Culture , In Vitro 10, 30, 1974
Rat Rat, SD, male, 150-200 g Myocytes Hyaluronidase: 0.05%
 (see reference) Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells , Am J Pathol 67 (2), 215, 1972
Rat Rat, albino, male, 250 g Myocytes Hyaluronidase: 0.20%
 Hank's solution, CF Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart , Circ Res 26, 679, 1970
Rat Rat, adult, male, 250 g Myocytes Trypsin: 0.1%
 Saline A Preparation of Beating Heart Cells From Adult Rats , Science 167, 1616, 1970
Rat Rat, Wistar, 1-4 day old Myocytes Trypsin: 0.125%
 Gey's BSS Pacemaker Activity and Mitosis in Cultures of Newborn Rat Heart Ventricle Cells , Exp Cell Res 44, 217, 1966
Rat Rat, SD, 1 day cardiomyocytes NCIS kit:
DMEM/F-12 Adenosine inhibits lipopolysaccharide-induced cardiac expression of tumor necrosis factor-alpha. , Circ Res 82, 47-56,
Shellfish Pacific Oysters Haemocytes Trypsin:
L15 medium Primary Culture of Pacific Oyster, Crassostrea gigas, heart cells, J Tiss Cul Meth 16, 67, 1994
Shellfish Clams, 3.0 to 4.5 cm Myocytes Fibroblasts Collagenase Type 1: 2%
 L15 medium Cultivation of Cells From the Heart of the Hard Clam, Merretrix lusoria (Roding), J Tiss Cul Meth 15, 123, 1993