Eye - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Microvascular endothelial Collagenase/Dispase: 0.1%
 MEM Primary Culture of Microvascular Endothelial Cells From Bovine Retina , In Vitro 18 (7), 626, 1982
Chicken Chick, embryo, 6 day retinal cells Trypsin: 0.005%
Deoxyribonuclease I: 0.005%
 DMEM/F12 Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions. , Cells Tissues Organs Vol. 180,, , 159-68, 2005
Chicken Chick embryo
(also rat)		 Retinal Trypsin: 0.6%
 DMEM The Golden Age of Retinal Cell Culture , Mol Vis 5, 4, 1999
Chicken Chick, embryo, 10-14 day Flat, retina Trypsin: 0.1%
 Tyrode's solution, CMF Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina , In Vitro Cell Dev Biol 26, 1073, 1990
Human human corneal limbal epithelial sheet Dispase: 5%
 see reference Novel enzymatic isolation of an entire viable human limbal epithelial sheet. , Inv Ophthal Visual Sci 44(10), 4275, 2003
Human Human, 5-65 years Retinal pigment epithelial (RPE) Trypsin: 0.25%
 HBSS Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture , Exp Eye Res 69, 97, 1999
Monkey monkey, cynomolgus, young adult conjunctival lymphocytes Collagenase Type 1: 0.02%
 RPMI 1640 Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection. , Infect Immun 57, 2977, 1989
Mouse mouse, 2-5 month mouse retinal and bipolar Papain: 20 u/ml
Deoxyribonuclease I: 200 u/ml
 HBSS Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission. , J Neurosci 25, 566-76, 2005
Mouse mouse, 4 week retinal endothelial cells Collagenase Type 1: 0.1%
 DMEM Isolation and characterization of murine retinal endothelial cells. , Mol Vis 9, 171, 2003
Porcine Porcine, 5-60 kg retinal pigment epithelial cells Collagenase: 2%
 DMEM Growth of Cultured Porcine Retinal Pigment Epithelial Cells , Acta Opthalmol Scand 81(2), 170, 2003
Rabbit Rabbit, New Zealand, adult, male, albino,
2 kg		 Epithelial Trypsin: 0.25%
 HBSS/DMEM Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions , Proc Natl Acad Sci U S A 75 (9), 4417, 1978
Rat rat, Dark Agouti, 3-4 week ciliary-derived eye PDS kit: with modifications
 DMEM/F12 Otx2 homeobox gene induces photoreceptor-specific phenotypes in cells derived from adult iris and ciliary tissue. , Inv Ophthal Visual Sci 45, 4570, 2004
Rat Rat, embryonic 17 day Retinal Trypsin: 0.6%
 DMEM The Golden Age of Retinal Cell Culture , Mol Vis 5, 4, 1999
Rat Rat, SD Retina Papain: 120 U
 HBSS, PBS GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF , Cell 85, 1113, 1996
Rat rat, 4-14 week retinal pigment epithelial cells Collagenase Type 1: 65 u/ml
Hyaluronidase: 220 u/ml
 CF Hanks with EDTA A method for the isolation of retinal pigment epithelial cells from adult rats. , Inv Ophthal Visual Sci 34(1), 101, 1993
Rat rat, 6-8 day retinal pigment epithelial Dispase: 2%
 DMEM An improved method for isolation and culture of pigment epithelial cells from rat retina. , Curr Eye Res 10(11), 1081, 1991
Rat Rat, Long Evans, 1-15 days Neurons, visual cortex Papain: 20 u/ml
 BSS see reference (see reference) Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats , J Neurosci 6, 3044, 1986
Rat Rat, SD, pups Retina Trypsin: 0.25%
 Ham's F-12 Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat , J Neurosci 3 (12), 2532, 1983
Rat Rat, SD, neonatal Retinal ganglion Trypsin: 0.25%
 CMF medium /Ham's F-10 with FCS Retrograde Labeling, Enrichment, and Characterization of Retinal Ganglion Cells from the Neonatal Rat , J Neurosci 3, 2532, 1983
Salamander Salamander, 18-25 cm Retina Papain: 14 u/ml
 Saline Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase , J Cell Biol 100, 175, 1985
Salamander Salamander (A. tigrinum) Photoreceptors, retina Papain: 0.05%
 (see reference) Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina , Proc Natl Acad Sci U S A 75, 3507, 1978
Turtle Turtle (Pseudemys scripta elegans) Retinal Papain: 0.1% (13.5 u/mg)
 Kreb's Ringer Biosynthesis of Acetylcholine in Turtle Photoreceptors , Proc Natl Acad Sci U S A 69, 1987, 1972