Epithelial - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine, fetal Epithelial Tracheal Dispase: 2%
 Dissociation medium, CMF Isolation, Characterization, and Long-Term Culture of Fetal Bovine Tracheal Epithelial Cells , In Vitro Cell Dev Biol 24, 211, 1988
Canine Dog Tracheal Pronase: 0.1%
 DMEM Evidence for Secretion of High Molecular Weight Mucins by Canine Tracheal Epithelial Cells in Primary Culture: Effects of Select Secretagogues on Mucin Secretion , In Vitro Cell Dev Biol 28A, 120, 1992
Chicken Chick, 5 day old Intestinal mesenchymal and epithelial Collagenase: 0.03%
 DMEM , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995
Fish Shark (Squalus acanthias) Rectal gland Collagenase: 0.2%
 Ringer's solution Atriopeptin Stimulates Chloride Secretion in Cultured Shark Rectal Gland Cells , Am J Physiol 260, 1125, 1991
Fish Shark (Squalus acanthias) Rectal gland Collagenase: 0.2%
 Ringer's solution , J Tiss Cul Meth 13, 149, 1991
Fish Winter flounder, 200-500 g
(Pseudopleuronectes americanus)		 Renal tubule Trypsin: 0.2%
 CMF solution Primary Culture of Flounder Renal Tubule Cells: Transepithelial Transport , Am J Physiol 251, 424, 1986
Frog Frog, Xenopus laevis, adult, female Colonic epithelial Collagenase Type 4: 0.1%
 Kreb's Potassium Conductances in Isolated Single Cells from Xenopus Laevis Colonic Epithelium , J Comp Physiol [B] 169, 148, 1999
Guinea-Pig Guinea-pig, Hartley, female, 200 g Epithelial Collagenase Type 1: 0.1%
 DMEM Use of Commerically Available Cell Culture Inserts for Primary Culture and Electrophysiologic Studies of Guinea Pig Gastric Mucous Epithelial Cells, J Tiss Cul Meth 14, 235, 1992
Guinea-Pig Guinea-pig, Hartley albino,
500-600 g		 Endometrial Collagenase: 0.25%
 HBSS Specific Effect of Oestrone Sulphate on Protein Synthesis and Secretion by Cultured Epithelial Cells from Guinea-pig Endometrium , J Endocrinol 123, 233, 1989
Hamster Hamster, Syrian gold, male, 100-120 g Tracheal Pronase: 0.1%
 MEM with Hepes, CMF Characterization Of Extended Primary And Secondary Cultures Of Hamster Tracheal Epithelial Cells , In Vitro Cell Dev Biol 24, 457, 1988
Hamster Hamster, Syrian golden (strain CR:RGH) Tracheal Trypsin: 0.1%
 Ham's F-12 Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters , Am J Pathol 129, 511, 1987
Hamster Hamster (strain 1516 EHS and Lakeview), 8-12 weeks Tracheal Trypsin: 0.05%
 Medium 199 Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture , Exp Lung Res 6, 27, 1984
Hamster Hamster, Syrian gold, male, 6 weeks- 4 months Tracheal Trypsin: 0.25%
 PBS with EDTA Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea , In Vitro 16, 313, 1980
Human human human tracheal epithelium Protease Type XIV: 0.04%
 DMEM/F12 Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function. , Biotechniques 39(2), 249, 2005
Human human corneal limbal epithelial sheet Dispase: 5%
 see reference Novel enzymatic isolation of an entire viable human limbal epithelial sheet. , Inv Ophthal Visual Sci 44(10), 4275, 2003
Human Human Gastric Collagenase Type 4: 0.01%
 F-12 medium , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:10.1, 1995
Human Human nasal polyp epithelial Dispase: .004%
Trypsin: 0.1%
 see reference Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells. , J Virol 69, 1473-9, 1995
Human Human Epithelial Trypsin: 0.05%
 DMEM Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands , In Vitro Cell Dev Biol 27A, 939, 1991
Human Human Epithelial Trypsin: 0.2%
 MEM, PBS Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13, 95, 1991
Human Human Epithelial Collagenase: 2.0%
 DMEM/Ham's F-12 Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples , In Vitro Cell Dev Biol 26, 1186, 1990
Human Human Epithelial Pronase: 0.1%
 PBS Long-Term Culture of Normal and Cystic Fibrosis Epithelial Cells Grown Under Serum-Free Conditions , In Vitro Cell Dev Biol 26, 411, 1990
Human Human Epithelial Sweat gland Collagenase Type 2: 0.2%
 (see reference) A Cystic Fibrosis Phenotype in Cells Cultured from Sweat Gland Secretory Coil. Altered Kinetics of 36Cl Efflux , J Biol Chem 265, 12796, 1990
Human Human
(also bovine)		 Endometrial epithelial Trypsin:
DMEM/Ham's F-12 Cultivation of Bovine Fetal and Adult Endometrial Epithelial Cells, J Tiss Cul Meth 11 (3), 129, 1988
Human Human Epithelial Deoxyribonuclease I: 0.01%
 HEPES with 5.9mM Glucose,5mM DTT Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa , J Appl Physiol 58, 1729, 1985
Human Human Epithelial Protease Type XIV: 0.1%
 Eagle's MEM Culture of Human Nasal Epithelial Cells on Collagen Matrix Supports , Am Rev Respir Dis 132, 1281, 1985
Human Human, women, 27-49 years Epithelial Ovary Trypsin: 0.125%
 HBSS, CMF Human Ovarian Surface Epithelium In Primary Culture , In Vitro 20, 743, 1984
Human Human, infant and neonate Epithelial Prostate Trypsin: 0.1%
 HBSS Normal Human Prostate Epithelial Cell Cultures , Methods Cell Biol 21, 195, 1980
Mouse mouse, 6-8 week lamina propria mononuclear cells Collagenase Type 2: 0.015%
Deoxyribonuclease I: 0.01%
 RPMI Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA. , J Immunol 168(1), 411, 2002
Mouse Mouse, fetal 12-13 day Intestinal mesenchymal and epithelial Collagenase: 0.03%
 DMEM , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995
Mouse Mouse Submandibular salivary Collagenase Type 2 or 3: 0.16%
 DMEM , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:2.1, 1995
Mouse Mouse Esophageal Trypsin: 0.25%
 PBS, CMF Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation , In Vitro Cell Dev Biol 27, 533, 1991
Mouse Mouse, mature, female, 6-8-wk-old Uterine Trypsin: 0.2%
 HBSS Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes , In Vitro Cell Dev Biol 27A, 713, 1991
Mouse Mouse, female, 18 - 20 days
(also 20 - 22 days)		 Uterine Trypsin: 0.25%
 HBSS Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum , In Vitro Cell Dev Biol 27A, 907, 1991
Mouse Mouse, (BALB/c), male, 3-5 months Epithelial Submandibular salivary gland Collagenase Type 3: 0.16% , 1:1 v/v
 DMEM with 15 mM HEPES Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix , In Vitro Cell Dev Biol 26, 33, 1990
Mouse Mouse, female Epithelial Pepsin: 0.1%
 HBSS Pepsin Can be Used to Subculture Viable Mammary Epithelial Cells , In Vitro 19 (9), 730, 1983
Mouse Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant Epithelial Submandibular gland Collagenase Type 3: 0.1%
 HBSS Growth in Primary Culture of Mouse Submandibular Epithelial Cells , In Vitro 18, 435, 1982
Mouse Mouse (BALB/cfC3H) Mammary tumors Epithelial Collagenase: 1.0%
 HBSS Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels , In Vitro 16, 502, 1980
Mouse Mouse, C3H, 6-8 weeks Epithelial Collagenase: 0.10%
 DMEM Establishment of Epithelial Cell Lines from Mouse Regenerating Liver , In Vitro 15, 910, 1979
Porcine Porcine, 5-60 kg retinal pigment epithelial cells Collagenase: 2%
 DMEM Growth of Cultured Porcine Retinal Pigment Epithelial Cells , Acta Opthalmol Scand 81(2), 170, 2003
Porcine Swine Trachea Dispase: 0.2%
 HBSS Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis , In Vitro 20, 433, 1984
Rabbit Rabbit, New Zealand white, adult Colon Dispase: 0.3%
 PBS A Method for the Rapid Establishment of Normal Adult Mammalian Colonic Epithelial Cell Cultures , In Vitro Cell Dev Biol 24 (3), 188, 1988
Rabbit Rabbit, New Zealand white, male, 4-5lb. Gastric Parietal and chief Collagenase Type 2: 0.08%
 Sodium phosphate buffer Release of Intracellular Ca2+ and Elevation of Inositol Triphosphate by Secretagogues in Parietal and Chief Cells Isolated from Rabbit Gastric Mucosa , Biochim Biophys Acta 888, 116, 1986
Rabbit Rabbit, New Zealand white estrous, female, 4-5 months Mesothelial and surface epithelial Ovaries Trypsin: 0.125%-0.5%
 Medium 199 Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) , Int J Gynecol Pathol 3, 348, 1984
Rabbit Rabbit, fetal Epithelial Gastric Collagenase Type 3: 0.10%
 HBSS Fetal Rabbit Gastric Epithelial Cells Cultured on Floating Collagen Gel , In Vitro 18, 233, 1981
Rat Rat, SD, adult, male, 8-10 weeks old Seminiferous tubules Trypsin: 0.05%
 Krebs-Ringer bicarbonate buffer
(see reference)		 Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization , Biol Proced Online 3 (1), 35, 2001
Rat rat, male alveolar epithelial Elastase: 40 u/ml
 DMEM Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair , J Cell Sci 112, 243, 1999
Rat rat, embryonic tracheal epithelial Collagenase Type 4: 0.05%
Dispase:
Deoxyribonuclease I:
DMEM/F12 Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture , Development 126, 1675, 1999
Rat Rat, fetal, 14-15 day Intestinal mesenchymal and epithelial Collagenase: 0.03%
 DMEM , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995
Rat rat, 4-14 week retinal pigment epithelial cells Collagenase Type 1: 65 u/ml
Hyaluronidase: 220 u/ml
 CF Hanks with EDTA A method for the isolation of retinal pigment epithelial cells from adult rats. , Inv Ophthal Visual Sci 34(1), 101, 1993
Rat Rat, SD, adult, 90-120 day old, 350-450 g Epididymal epithelial Collagenase Type 2: 0.1%
 HBSS A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats, J Tiss Cul Meth 14, 195, 1992
Rat rat, 6 day old rat intestinal epithelial Dispase: 0.01%
Collagenase: 300 u/ml
 DMEM The development of a method for the preparation of rat intestinal epithelial cell primary cultures. , J Cell Sci 101, 219, 1992
Rat Rat, SD, male, 150 - 250 g Epithelial Stomach Pronase: 0.15%
 Medium 199 Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture , In Vitro Cell Dev Biol 27, 39, 1991
Rat Rat, adult
(also hamster)		 Interlobular duct fragments Papain:
DMEM/Ham's F-12 Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix , Pancreas 6 (5), 514, 1991
Rat Rat, SD, 200 g Colon Deoxyribonuclease I: 10 µg/ml
 (see reference) Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro , Peptides 12, 63, 1991
Rat Rat, Wistar, neonatal Epithelial Trypsin: 0.1%
 HBSS Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium , In Vitro Cell Dev Biol 27A, 625, 1991
Rat Rodent, various (see reference) Epithelial Trypsin: 0.2%
 MEM, PBS Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13, 95, 1991
Rat rat, 6-8 day retinal pigment epithelial Dispase: 2%
 DMEM An improved method for isolation and culture of pigment epithelial cells from rat retina. , Curr Eye Res 10(11), 1081, 1991
Rat Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat (ACI/MNs) male, 8 weeks		 Epthelial Collagenase Type 3: 0.1%
 Eagle's MEM Serum-free Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus , In Vitro Cell Dev Biol 26, 713, 1990
Rat Rat, Fisher 344, male, 8 wks old,
250-300 g		 Tracheal epithelial Pronase: 0.5%
 DMEM Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture , J Cell Sci 74, 283, 1985
Rat Rat, Fischer, male, 4-6 weeks Epithelial Esophagus Hyaluronidase: 0.1%
 HEPES BSS Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells , In Vitro 19, 403, 1983
Rat Rat, Fischer, Lewis and SD, male, 10-18 months Epithelial Trypsin: 0.05%
 CMF HBSS Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats , In Vitro 19, 576, 1983
Rat Rat, Wistar, 12 day Epithelial Trypsin: 0.05%
 HBSS, CMF Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels , Exp Cell Res 131, 363, 1981
Rat Rat, Fischer, adult, 200-250 g Epithelial Hyaluronidase: 0.0075%
 KCl-NaCl HEPES Buffer Long-Term Culture of Adult Rat Liver Epithelial Cells , Exp Cell Res 89, 139, 1974
Rat Rat, Fischer, 10 day Epithelial-like Trypsin: 0.25%
 PBS Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver , Exp Cell Res 69, 106, 1971