Epithelial - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine, fetal Epithelial
Tracheal		 Neutral Protease: 2%
 Dissociation medium, CMF Schumann, B.L., Cody, T.E., Miller, M.L., Leikauf, G.D.: Isolation, Characterization, and Long-Term Culture of Fetal Bovine Tracheal Epithelial Cells, In Vitro Cell Dev Biol 24, 211, 1988
Canine Dog Tracheal Pronase: 0.1%
 DMEM Virmani, A., Naziruddin, B., Desai, V., Lowry, J., Graves, D., and Sachdev, G.: Evidence for Secretion of High Molecular Weight Mucins by Canine Tracheal Epithelial Cells in Primary Culture: Effects of Select Secretagogues on Mucin Secretion, In Vitro Cell Dev Biol 28A, 120, 1992
Chicken Chick, 5 day old Intestinal mesenchymal and epithelial Collagenase: 0.03%
 DMEM Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995
Fish Shark (Squalus acanthias) Rectal gland Collagenase: 0.2%
 Ringer's solution Karnaky, Jr., K.J., Valentich, J.D., Currie, M.G., Oehlenschlager, W.F., and Kennedy, M.P.: Atriopeptin Stimulates Chloride Secretion in Cultured Shark Rectal Gland Cells, Am J Physiol 260, 1125, 1991
Fish Shark (Squalus acanthias) Rectal gland Collagenase: 0.2%
 Ringer's solution Valentich, J: J Tiss Cul Meth 13, 149, 1991
Fish Winter flounder, 200-500 g
(Pseudopleuronectes americanus)		 Renal tubule Trypsin: 0.2%
 CMF solution Dickman, K.G., and Renfro, J.: Primary Culture of Flounder Renal Tubule Cells: Transepithelial Transport, Am J Physiol 251, 424, 1986
Frog Frog, Xenopus laevis, adult, female Colonic epithelial Collagenase Type 4: 0.1%
 Kreb's Heinke, B, and Clauss, W.: Potassium Conductances in Isolated Single Cells from Xenopus Laevis Colonic Epithelium, J Comp Physiol [B] 169, 148, 1999
Guinea-Pig Guinea-pig, Hartley, female, 200 g Epithelial Collagenase Type 1: 0.1%
 DMEM Rutten, M.: Use of Commerically Available Cell Culture Inserts for Primary Culture and Electrophysiologic Studies of Guinea Pig Gastric Mucous Epithelial Cells, J Tiss Cul Meth 14, 235, 1992
Guinea-Pig Guinea-pig, Hartley albino,
500-600 g		 Endometrial Collagenase: 0.25%
 HBSS Chaminadas, G., Alkhalaf, M., Remy-Martin, J.P., Propper, A.Y., and Adessi, G.L.: Specific Effect of Oestrone Sulphate on Protein Synthesis and Secretion by Cultured Epithelial Cells from Guinea-pig Endometrium, J Endocrinol 123, 233, 1989
Hamster Hamster, Syrian gold, male, 100-120 g Tracheal Pronase: 0.1%
 MEM with Hepes, CMF Niles,R., Kim, K.C., Hyman, B., Christensen, T., Wasano, K., Brody, J.: Characterization Of Extended Primary And Secondary Cultures Of Hamster Tracheal Epithelial Cells, In Vitro Cell Dev Biol 24, 457, 1988
Hamster Hamster, Syrian golden (strain CR:RGH) Tracheal Trypsin: 0.1%
 Ham's F-12 McDowell, E., et al.: Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters, Am J Pathol 129, 511, 1987
Hamster Hamster (strain 1516 EHS and Lakeview), 8-12 weeks Tracheal Trypsin: 0.05%
 Medium 199 Lee, T., Wu, R., Brody, A., Barrett, J., and Nettesheim, P.: Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture, Exp Lung Res 6, 27, 1984
Hamster Hamster, Syrian gold, male, 6 weeks- 4 months Tracheal Trypsin: 0.25%
 PBS with EDTA Goldman, W.E., Baseman, J.B.: Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea, In Vitro 16, 313, 1980
Human Human Human tracheal epithelium Protease Type XIV: 0.04%
 DMEM/F12 Widdicombe JH, Sachs LA, Morrow JL, and Finkbeiner WE.: Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function, Biotechniques 39(2), 249, 2005
Human Human colonic epithelial Collagenase:
Neutral Protease: 0.3%
Deoxyribonuclease I: 0.05%
 RPMI 1640 Fukushima Kouhei, Fiocchi Claudio: Paradoxical decrease of mitochondrial DNA deletions in epithelial cells of active ulcerative colitis patients.,Am J Physiol Gastrointest Liver Physiol Vol. 286,, , G804-13, 2004
Human Human Corneal limbal epithelial sheet Neutral Protease: 5%
 see reference Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R, and Tseng SC: Novel enzymatic isolation of an entire viable human limbal epithelial sheet, Inv Ophthal Visual Sci 44(10), 4275, 2003
Human Human Gastric Collagenase Type 4: 0.01%
 F-12 medium Sarosiek, J, Marshall, B, Peura, D, Guerrant, L, McCallum, R and Little, C: The Isolation and Maintenance of Human Gastric Epithelial Cells in Primary Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:10.1, 1995
Human Human Nasal polyp epithelial Neutral Protease: .004%
Trypsin: 0.1%
 see reference Halbert CL, Alexander IE, Wolgamot GM, Miller AD: Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells, J Virol 69, 1473-9, 1995
Human Human Epithelial Trypsin: 0.05%
 DMEM Sabatini, L., Allen-Hoffmann, B, Warner, T., and Azen, E.: Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands, In Vitro Cell Dev Biol 27A, 939, 1991
Human Human Epithelial Trypsin: 0.2%
 MEM, PBS Robinson, C., and Wu, R.: Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13, 95, 1991
Human Human Epithelial Collagenase: 2.0%
 DMEM/Ham's F-12 Emerman, J. and Wilkinson, D.: Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples, In Vitro Cell Dev Biol 26, 1186, 1990
Human Human Epithelial Pronase: 0.1%
 PBS Gruenert, D.C., Basbaum, C.B., and Widdicombe, J.H.: Long-Term Culture of Normal and Cystic Fibrosis Epithelial Cells Grown Under Serum-Free Conditions, In Vitro Cell Dev Biol 26, 411, 1990
Human Human Epithelial
Sweat gland		 Collagenase Type 2: 0.2%
 (see reference) Wood, L. and Neufeld, E.: A Cystic Fibrosis Phenotype in Cells Cultured from Sweat Gland Secretory Coil. Altered Kinetics of 36Cl Efflux, J Biol Chem 265, 12796, 1990
Human Human
(also bovine)		 Endometrial epithelial Trypsin:
DMEM/Ham's F-12 Munson, L., Chandler, S., and Schlafer, D.: Cultivation of Bovine Fetal and Adult Endometrial Epithelial Cells, J Tiss Cul Meth 11 (3), 129, 1988
Human Human Epithelial Deoxyribonuclease I: 0.01%
 HEPES with 5.9mM Glucose,5mM DTT Widdicombe, J.H., Coleman, D.L., Finkbeiner, W.E., and Tuet, I.K.: Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa, J Appl Physiol 58, 1729, 1985
Human Human Epithelial Protease Type XIV: 0.1%
 Eagle's MEM Yankaskas, J., Cotton, C., Knowles, M., Gatzy, J., and Boucher, R.: Culture of Human Nasal Epithelial Cells on Collagen Matrix Supports, Am Rev Respir Dis 132, 1281, 1985
Human Human, women, 27-49 years Epithelial
Ovary		 Trypsin: 0.125%
 HBSS, CMF Auersperg, N., Siemens, C.H., and Myrdal, S.E.: Human Ovarian Surface Epithelium In Primary Culture, In Vitro 20, 743, 1984
Human Human, infant and neonate Epithelial
Prostate		 Trypsin: 0.1%
 HBSS Lechner, J., Babcock, M., Marnell, M., Narayan, K., and Kaighn, M.: Normal Human Prostate Epithelial Cell Cultures, Methods Cell Biol 21, 195, 1980
Mouse Mouse, 6-8 week Lamina propria mononuclear cells Collagenase Type 2: 0.015%
Deoxyribonuclease I: 0.01%
 RPMI Wirtz S., Becker C., Blumberg R., Galle P., and Neurath M.: Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA, J Immunol 168(1), 411, 2002
Mouse Mouse, female Salivary gland epithelial Collagenase Type 1: 750 u/ml
Hyaluronidase: 500 u/ml
 DMEM/F12 Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y: Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis, Am J Pathol 155, 173-81, 1999
Mouse Mouse, fetal 12-13 day Intestinal mesenchymal and epithelial Collagenase: 0.03%
 DMEM Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995
Mouse Mouse Submandibular salivary Collagenase Type 2 or 3: 0.16%
 DMEM Durban, E: Submandibular Salivary Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:2.1, 1995
Mouse Mouse Esophageal Trypsin: 0.25%
 PBS, CMF Katayama, M., Kan, M.: Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation, In Vitro Cell Dev Biol 27, 533, 1991
Mouse Mouse, mature, female, 6-8-wk-old Uterine Trypsin: 0.2%
 HBSS Ghosh, D., Danielson, K., Alston, J., and Heyner, S.: Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes, In Vitro Cell Dev Biol 27A, 713, 1991
Mouse Mouse, female, 18 - 20 days
(also 20 - 22 days)		 Uterine Trypsin: 0.25%
 HBSS Fukamachi, H., and McLachlan, J.: Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum, In Vitro Cell Dev Biol 27A, 907, 1991
Mouse Mouse, (BALB/c), male, 3-5 months Epithelial
Submandibular salivary gland		 Collagenase Type 3: 0.16% , 1:1 v/v
 DMEM with 15 mM HEPES Durban, E.M.: Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix, In Vitro Cell Dev Biol 26, 33, 1990
Mouse Mouse, female Epithelial Pepsin: 0.1%
 HBSS Reiser, M., Huff, B., and Medina, D.: Pepsin Can be Used to Subculture Viable Mammary Epithelial Cells, In Vitro 19 (9), 730, 1983
Mouse Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant Epithelial
Submandibular gland		 Collagenase Type 3: 0.1%
 HBSS Yang, J., Flynn, D., Larson, L., and Hamamoto, S.: Growth in Primary Culture of Mouse Submandibular Epithelial Cells, In Vitro 18, 435, 1982
Mouse Mouse (BALB/cfC3H) Mammary tumors
Epithelial		 Collagenase: 1.0%
 HBSS Yang, J., Guzman, R., Richards, J., and Nandi, S.: Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels, In Vitro 16, 502, 1980
Mouse Mouse, C3H, 6-8 weeks Epithelial Collagenase: 0.10%
 DMEM Lillehaug, J., Mondal, S., and Heidelberger, C.: Establishment of Epithelial Cell Lines from Mouse Regenerating Liver, In Vitro 15, 910, 1979
Porcine Porcine, 5-60 kg Retinal pigment epithelial cells Collagenase: 2%
 DMEM Wiencke, A., Kiilgaard, J., Nicolini, J., Bundgaard, M., Ropke, C., and La Cour, M.: Growth of Cultured Porcine Retinal Pigment Epithelial Cells, Acta Opthalmol Scand 81(2), 170, 2003
Porcine Porcine Trachea Neutral Protease: 0.2%
 HBSS De Buysscher, E., Kennedy, J., and Mendicino, J.: Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis, In Vitro 20, 433, 1984
Rabbit Rabbit, New Zealand white, adult Colon Neutral Protease: 0.3%
 PBS Vidrich, A., Racindranath, R., Farsi, K., and Targan, S.: A Method for the Rapid Establishment of Normal Adult Mammalian Colonic Epithelial Cell Cultures, In Vitro Cell Dev Biol 24 (3), 188, 1988
Rabbit Rabbit, New Zealand white, male, 4-5lb. Gastric
Parietal and chief		 Collagenase Type 2: 0.08%
 Sodium phosphate buffer Chew, C.S., Brown,M.R.: Release of Intracellular Ca2+ and Elevation of Inositol Triphosphate by Secretagogues in Parietal and Chief Cells Isolated from Rabbit Gastric Mucosa, Biochim Biophys Acta 888, 116, 1986
Rabbit Rabbit, New Zealand white estrous, female, 4-5 months Mesothelial and surface epithelial
Ovaries		 Trypsin: 0.125%-0.5%
 Medium 199 Nicosia, S., Johnson, J., and Streibel, E.: Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium), Int J Gynecol Pathol 3, 348, 1984
Rabbit Rabbit, fetal Epithelial
Gastric		 Collagenase Type 3: 0.10%
 HBSS Logsdon, C.D., Bisbee, C.A., Rutten, M.J. and Machen, T.E.: Fetal Rabbit Gastric Epithelial Cells Cultured on Floating Collagen Gel, In Vitro 18, 233, 1981
Rat Rat, SD, adult, male, 8-10 weeks old Seminiferous tubules Trypsin: 0.05%
 Krebs-Ringer bicarbonate buffer
(see reference)		 Abou-Haila, A., and Tulsiani, D.: Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization, Biol Proced Online 3 (1), 35, 2001
Rat Rat, male Alveolar epithelial Elastase: 40 u/ml
 DMEM Planus, E., Galiacy, S., Matthay, M., Laurent, V., Gavrilovic, J., Murphy, G., Clerici, C., Isabey, D., Lafuma, C., and d'Ortho, M.: Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair, J Cell Sci 112, 243, 1999
Rat Rat, embryonic Tracheal epithelial Collagenase Type 4: 0.05%
Neutral Protease:
Deoxyribonuclease I:
DMEM/F12 Shannon JM, Gebb SA, and Nielsen LD: Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture, Development 126, 1675, 1999
Rat Rat, fetal, 14-15 day Intestinal mesenchymal and epithelial Collagenase: 0.03%
 DMEM Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995
Rat Rat, 4-14 week Retinal pigment epithelial cells Collagenase Type 1: 65 u/ml
Hyaluronidase: 220 u/ml
 CF Hanks with EDTA Wang N, Koutz CA, and Anderson RE.: A method for the isolation of retinal pigment epithelial cells from adult rats, Inv Ophthal Visual Sci 34(1), 101, 1993
Rat Rat, SD, adult, 90-120 day old, 350-450 g Epididymal epithelial Collagenase Type 2: 0.1%
 HBSS Klinefelter, G.: A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats, J Tiss Cul Meth 14, 195, 1992
Rat Rat, 6 day old Rat intestinal epithelial Neutral Protease: 0.01%
Collagenase: 300 u/ml
 DMEM Evans GS, Flint N, Somers AS, Eyden B, and Potten CS.: The development of a method for the preparation of rat intestinal epithelial cell primary cultures, J Cell Sci 101, 219, 1992
Rat Rat, SD, male, 150 - 250 g Epithelial
Stomach		 Pronase: 0.15%
 Medium 199 Dial, E., Kao, Y., and Lichtenberger, L.: Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture, In Vitro Cell Dev Biol 27, 39, 1991
Rat Rat, adult
(also hamster)		 Interlobular duct fragments Papain:
DMEM/Ham's F-12 Heimann, T., and Githens, S.: Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix, Pancreas 6 (5), 514, 1991
Rat Rat, SD, 200 g Colon Deoxyribonuclease I: 10 µg/ml
 (see reference) Yassin, R., Clearfield, H., Katz, S., and Murthy, S.: Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro, Peptides 12, 63, 1991
Rat Rat, Wistar, neonatal Epithelial Trypsin: 0.1%
 HBSS Jassal, D., Han, R., Caniggia, I., Post, M., and Tanswell, A.: Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium, In Vitro Cell Dev Biol 27A, 625, 1991
Rat Rodent, various (see reference) Epithelial Trypsin: 0.2%
 MEM, PBS Robinson, C., and Wu, R.: Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13, 95, 1991
Rat Rat, 6-8 day Retinal pigment epithelial Neutral Protease: 2%
 DMEM Chang CW, Roque RS, Defoe DM, and Caldwell RB.: An improved method for isolation and culture of pigment epithelial cells from rat retina, Curr Eye Res 10(11), 1081, 1991
Rat Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat (ACI/MNs) male, 8 weeks		 Epthelial Collagenase Type 3: 0.1%
 Eagle's MEM Serum-free Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus, In Vitro Cell Dev Biol 26, 713, 1990
Rat Rat, Fisher 344, male, 8 wks old,
250-300 g		 Tracheal epithelial Pronase: 0.5%
 DMEM Chang, L., Wu, R., and Nettesheim, P.: Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture, J Cell Sci 74, 283, 1985
Rat Rat, Fischer, male, 4-6 weeks Epithelial
Esophagus		 Hyaluronidase: 0.1%
 HEPES BSS Babcock, M., Marino, M., Gunning, W., and Stoner, G.: Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells, In Vitro 19, 403, 1983
Rat Rat, Fischer, Lewis and SD, male, 10-18 months Epithelial Trypsin: 0.05%
 HBSS CMF Herring, A., Raychaudhuri, R., Kelley, S., and Iybe, P.: Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats, In Vitro 19, 576, 1983
Rat Rat, SD, female Epithelial, cancer and tumor Collagenase: 0.1%
 Eagles's MEM Cohen, L.: Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma, In Vitro 18, 565, 1982
Rat Rat, Wistar, 12 day Epithelial Trypsin: 0.05%
 HBSS, CMF Malan-Shibley, L., and Iype, P.: Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels, Exp Cell Res 131, 363, 1981
Rat Rat, Fischer, adult, 200-250 g Epithelial Hyaluronidase: 0.0075%
 KCl-NaCl HEPES Buffer Williams, G., and Gunn, J.: Long-Term Culture of Adult Rat Liver Epithelial Cells, Exp Cell Res 89, 139, 1974
Rat Rat, Fischer, 10 day Epithelial-like Trypsin: 0.25%
 PBS Williams, G., Weisburger, E., and Weisburger, J.: Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver, Exp Cell Res 69, 106, 1971