| Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
| Bovine
|
Bovine, (Bos taurus), calf |
Endothelial
Pulmonary |
Collagenase: 1000 u/ml
|
PBS, CMF |
Culture and Characterization of Pulmonary Microvascular Endothelial Cell
,
In Vitro Cell Dev Biol
28A,
711, 1992 |
| Bovine
|
Calf |
Endothelial |
Trypsin: 0.25%
|
HEPES |
Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension
,
In Vitro Cell Dev Biol
28A,
403, 1992 |
| Bovine
|
Bovine, adult |
Cerebral artery
Endothelial |
Collagenase: 0.2%
|
HBSS |
Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries
,
In Vitro Cell Dev Biol
26,
291, 1990 |
| Bovine
|
Bovine |
Endothelial
Aortic |
Trypsin: 0.05%
|
Krebs Ringer solution |
Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled
,
Proc Natl Acad Sci U S A 85,
2334, 1988 |
| Bovine
|
Bovine |
Endothelial, pulmonary artery |
Collagenase: 0.1%
|
CMF-Dulbecco's PBS |
Formation of Diacylglycerol by a Phospholipase D-phosphatidate Phosphatase Pathway Specific for Phosphatidylcholine in Endothelial Cells
,
Biochim Biophys Acta
962,
282, 1988 |
| Bovine
|
Bovine |
Endothelial
Aorta |
Collagenase Type 2: 0.1%
|
PBS |
Microvascular Endothelium and Pericytes: High Yield, Low Passage Cultures
,
In Vitro Cell Dev Biol
22,
344, 1986 |
| Bovine
|
Bovine |
Endothelial
Aortic |
Collagenase Type 2: 0.1%
|
DMEM |
Modulation of Sulfated Proteoglycan Synthesis by Bovine Aortic Endothelial Cells During Migration
,
J Cell Biol
102,
679, 1986 |
| Bovine
|
Bovine |
Endothelial
Brain arteries |
Collagenase Type 2: 0.2%
|
Dulbecco's PBS |
Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain
,
In Vitro Cell Dev Biol
21,
172, 1985 |
| Bovine
|
Calf |
Endothelial
Smooth muscle |
Collagenase: 0.75%
|
DMEM |
Identification and Isolation of Endothelial Cells Based on Their Increased Uptake of Acetylated-Low Density Lipoprotein
,
J Cell Biol
99,
2034, 1984 |
| Bovine
|
Bovine |
Endothelial
Corneal |
Trypsin: 0.2%
|
PBS: DMEM |
Investigation of the Attachment of Bovine Corneal Endothelial Cells
,
Exp Cell Res
144,
472, 1983 |
| Bovine
|
Bovine, 2-3 weeks |
Endothelial
Pulmonary artery |
Collagenase Type 1: 0.2%
|
RPMI 1640 w/1% Fetal Bovine Serum |
Ultrastructural Changes in Bovine Pulmonary Artery Endothelial Cells Exposed to 80% O2 In Vitro
,
In Vitro 19,
714, 1983 |
| Bovine
|
Bovine |
Endothelial, corneal |
Trypsin: 0.05%
|
0.01M Phosphate buffer with 0.02% EDTA, 0.9% NaCl (See reference) |
Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells
,
J Cell Physiol
117,
368, 1983 |
| Bovine
|
Bovine |
Endothelial
Subclavian vein |
Collagenase Type 1: 0.10%
|
PBS |
An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells.
,
In Vitro 18,
99, 1982 |
| Bovine
|
Bovine |
Aortic
Pulmonary artery |
Collagenase Type 2: 0.10%
|
PBS |
Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells.
,
In Vitro 17,
450, 1981 |
| Bovine
|
Calf, fetal, 4-9 months |
Endothelial |
Collagenase Type 1: 0.25%
|
PBS |
Proliferative Characteristics of Clonal Endothelial Cell Strains
,
J Cell Physiol
107,
123, 1981 |
| Bovine
|
Bovine, adult |
Aorta |
Collagenase Type 1: 125 u/ml
|
Dulbecco's PBS with calcium and magnesium |
Studies of Morphologically Atypical ("Sprouting") Cultures of Bovine Aortic Endothelial Cells. Growth Characteristics and Connective Tissue Protein Synthesis
,
J Cell Physiol
102,
183, 1980 |
| Bovine
|
Bovine, young |
Pulmonary artery |
Collagenase: 0.1%
|
Medium 199 |
Methods for Microcarrier Culture of Bovine Pulmonary Artery Endothelial Cells Avoiding the Use of Enzymes
,
Tissue Cell
12,
619, 1980 |
| Bovine
|
Bovine |
Foreskin |
Collagenase: 0.5%
|
Dulbecco's modified Eagle's medium w/10% calf serum |
Long-term Culture of Capillary Endothlial Cells
,
Proc Natl Acad Sci U S A 76,
5217, 1979 |
| Bovine
|
Bovine |
Saphenous Vein
Aorta |
Collagenase: 0.01%
|
PBS |
Comparison of Tissue-Cultured Bovine Endothelial Cells from Aorta and Saphenous Vein
,
In Vitro 14,
903, 1978 |
| Bovine
|
Bovine |
Endothelial
Thoracic aorta
Saphenous veins |
Collagenase Type 2: 0.1%
|
PBS |
Selection and Characterization of Bovine Aortic Endothelial Cells
,
In Vitro 14,
966, 1978 |
| Bovine
|
Bovine |
Pulmonary artery |
Collagenase Type 2: 0.25%
|
Puck's solution |
Isolation And Culture of Pulmonary Artery Endothelial Cells
,
Tissue Cell
10,
535, 1978 |
| Bovine
|
Calf |
Endothelial |
Collagenase Type 2: 0.1%
|
DMEM |
Characterization of the Collagen Synthesized by Endothelial Cells in Culture
,
Proc Natl Acad Sci U S A 73 (7),
2361, 1976 |
| Canine
|
Dog
(also human) |
Microvessels |
Collagenase Type 4: 0.1%
|
DMEM |
Cutlured Human and Canine Endothelial Cells from Brain Microvessels
,
Brain Res Bull 21,
785, 1988 |
| Canine
|
Dog, mongrel, adult |
Endothelium
Jugular vein |
Trypsin: 0.1%
|
Earle's PBS, CMF |
Isolation of Adult Canine Venous Endothelium for Tissue Culture
,
In Vitro 17,
44, 1981 |
| Guinea-Pig
|
guinea pig, female, 300-350g |
coronary endothelial |
Collagenase Type 2: 0.1%
|
see reference |
Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium.
,
Am J Physiol Heart Circ Physiol
281,
H1657-66, 2001 |
| Human
|
Human |
HUVEC |
Collagenase Type 4: 0.1%
|
RPMI 1640 |
Neuropeptide Y expression, localization and cellular transducing effects in HUVEC
,
Biol Cell 97(6),
457, 2005 |
| Human
|
Human |
colonic endothelial cells |
Collagenase Type 2: 0.25%
|
HBSS/5%FBS |
Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells
,
Am J Physiol/Gastro 282,
G1088, 2002 |
| Human
|
human |
pulmonary vascular endothelial cells |
Dispase: 1.18 u/ml
Elastase: 10 u/ml
|
M199 |
Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro.
,
Exp Mol Pathol 72,
221, 2002 |
| Human
|
Human |
Endothelial |
Trypsin: 2%
|
PBS |
Comparsion of the Rates of Deamidation, Diketopiperazine Formation and Oxidation in Recombinant Human Vascular Endothelial Growth Factor and Model Peptides
,
AAPS PharmSci
2 (1),
5, 2000 |
| Human
|
Human |
HUVEC, porcine pulmonary arterial endothelial cells |
Collagenase Type 2: 0.2%
|
DMEM |
Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells.
,
Circulation 101(19),
2317, 2000 |
| Human
|
Human |
hepatic endothelial cells |
Collagenase: 0.2%
|
DMEM |
A simplified method for the isolation and culture of endothelial cells from pseudointima of transjugular intrahepatic portasystemic shunts.
,
Lab Invest 78(11),
1469, 1998 |
| Human
|
Human |
Endothelial |
Trypsin: 0.3.%
|
HBSS
(see reference) |
VCAM-1-, ELAM-1-, and ICAM-1-Independent Adhesion of Melanoma Cells to Cultured Human Dermal Microvascular Endothelial Cells
,
J Invest Dermatol 98,
79, 1992 |
| Human
|
human |
vascular endothelial |
Dispase: 0.15%
Trypsin: 0.25%
|
M-199 |
Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis.
,
Am J Physiol 262,
H1088-95, 1992 |
| Human
|
Human |
Umbilical vein
HUVE |
Collagenase: 0.1%
|
Cord buffer
(see reference) |
Intracellular Mechanisms Involved in Basement Membrane Induced Blood Vessel Differentiation In Vitro
,
In Vitro Cell Dev Biol
27,
327, 1991 |
| Human
|
Human |
Crypt cells |
Collagenase: 125 u/ml
|
RMPI 1640 |
A Method For the Prolonged Culture of Colonic Epithelial Cells,
J Tiss Cul Meth
13,
103, 1991 |
| Human
|
Human |
Endothelial |
Collagenase Type 1: 0.2%
|
Medium 199 |
Migration Assay for Endothelial Cells in Multiwells Application to Studies on the Effect of Opioids
,
J Immunol Methods 128,
235, 1990 |
| Human
|
Human |
Human umbilical vein endothelial cells |
Collagenase Type 2: 75 u/ml
|
M199 |
A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions.
,
J Exp Med 170,
399-414, 1989 |
| Human
|
Human |
Microvessels |
Collagenase Type 4: 0.1%
|
DMEM |
Cutlured Human and Canine Endothelial Cells from Brain Microvessels
,
Brain Res Bull 21,
785, 1988 |
| Human
|
Human |
Endothelial/HUVE
Foreskin & umbilical cord |
Trypsin: 0.3%
|
HBSS/PBS, Medium 199
(see reference) |
Role of Laminin and Basement Membrane in Morphological Differentiation of Human Endothelial Cells into Capillary-like Structures
,
J Cell Biol
107,
1589, 1988 |
| Human
|
Human |
Umbilical cord
Smooth muscle |
Collagenase: 0.1%
|
HEPES |
Comparative Endocrinology-Paracrinology-Autocrinology of Human Adult Large Vessel Endothelial and Smooth Muscle Cells
,
In Vitro Cell Dev Biol
24 (4),
309, 1988 |
| Human
|
Human |
Endothelial
Saphenous vein |
Collagenase Type 2: 0.1%
|
PBS, CMF |
The Cytoxic Effect of Surgical Glove Powder Particles on Adult Human Vascular Endothelial Cell Cultures: Implications for Clinical Uses of Tissue Culture Techniques
,
J Surg Res 41,
463, 1986 |
| Human
|
Human |
Endothelial |
Collagenase: 0.1%
|
HEPES |
Isolation, Growth Requirements, Cloning, Prostacylclin Production and Life-Span of Human Adult Endothelial Cells in Low Serum Culture Medium
,
In Vitro Cell Dev Biol
22 (1),
51, 1986 |
| Human
|
Human |
Endothelial
Dermal |
Trypsin: 0.3%
|
PBS |
Human Dermal Microvascular Endothelial Cells: An Improved Method for Tissue Culture and Description of Some Singular Properties in Culture
,
In Vitro Cell Dev Biol
21,
627, 1985 |
| Human
|
Human |
Fibroblasts
Foreskin |
Hyaluronidase: 0.10%
|
DMEM |
Long-Term Culture of Human Endothelial Cells
,
In Vitro 19,
661, 1983 |
| Human
|
Human |
Iliac arteries |
Collagenase: 0.25%
|
PBS w/Ca++, Mg++, & BSA
(see reference) |
Cultured Endothelial Cells Derived from the Human Iliac Arteries
,
In Vitro 18,
859, 1982 |
| Human
|
Human, adult |
Peripheral blood mononuclear
Monocytes
T cells
Endothelial |
Collagenase: 0.25%
|
RPMI 1640 |
Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro.
,
J Clin Invest 67,
1490, 1981 |
| Human
|
Human |
Microvascular endothelial
Neonatal foreskins |
Dispase: at 1000 u/ml
|
Konigsberg's modification of HBSS
(see reference) |
Cultivation of Microvascular Endothelial Cells from Human Preputial Skin
,
In Vitro 16,
675, 1980 |
| Human
|
Human (adult and child) |
Foreskin |
Collagenase: 0.5%
|
Dulbecco's modified Eagle's medium w/10% calf serum |
Long-term Culture of Capillary Endothlial Cells
,
Proc Natl Acad Sci U S A 76,
5217, 1979 |
| Human
|
Human |
Umbilical vein
|
Trypsin: 100 µg/ml
|
Tris-HCl,0.2 M |
Synthesis of Basement Membrane Collagen By Cultured Human Endothelial Cells
,
J Exp Med 144,
209, 1976 |
| Human
|
Human |
Umbilical vein |
Collagenase Type 1: 125 u/ml
|
Dulbecco's PBS |
Culture of Vascular Endothelium
,
Progress in Hemostasis and Thrombosis Vol. 3,(Spaet,T.H.,ed.),
Grune and Stratton, NY,
1, 1976 |
| Human
|
Human |
Umbilical cord |
Collagenase: 0.1%
|
Dulbecco's PBS |
Human Vascular Endothelial Cells in Culture: Growth and DNA Synthesis
,
J Cell Biol
60,
673, 1974 |
| Human
|
Human |
Umbilical cord |
Collagenase: 0.2%
|
Cord buffer
(see reference) |
Culture of Human Endothelial Cells Derived from Umbilical Veins. Identification by Morphologic and Immunologic Criteria
,
J Clin Invest 52,
2745, 1973 |
| Human
|
Human |
Umbilical cord |
Trypsin NF 1:250: 0.125%
|
Saline, normal |
Replication of Human Endothelial Cells in Culture
,
Science 181,
452, 1973 |
| Human
|
Human |
Umbilical cord |
Trypsin NF 1:250: 0.25%
|
CMF solution |
Human Endothelium in Cell Culture
,
J Atheroscler Res 6,
151, 1966 |
| Mouse
|
mouse, embryonic or yolk sac |
endothelial |
Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 0.001%
|
PBS |
Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation.
,
J Cell Biol
172,
151-62, 2006 |
| Mouse
|
mouse, 4 week |
retinal endothelial cells |
Collagenase Type 1: 0.1%
|
DMEM |
Isolation and characterization of murine retinal endothelial cells.
,
Mol Vis 9,
171, 2003 |
| Mouse
|
mouse, male |
endothelial cells from lymph node |
Collagenase Type 1: 0.1%
|
PBS |
Expression profile of active genes in mouse lymph node high endothelial cells.
,
Int Immunol 11(12),
1989, 1999 |
| Porcine
|
Porcine
(also bovine) |
Endothelial |
Collagenase Type 1:
|
DMEM w/ 10% calf serum |
Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury
,
J Surg Res 99,
228, 2001 |
| Porcine
|
porcine |
HUVEC, porcine pulmonary arterial endothelial cells |
Collagenase Type 2: 0.2%
|
DMEM |
Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells.
,
Circulation 101(19),
2317, 2000 |
| Porcine
|
pig, 6-7 month |
porcine pulmonary endothelial |
Collagenase Type 1: 0.3%
|
RPMI 1640 |
Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV.
,
J Am Soc Nephrol
10(3),
481, 1999 |
| Porcine
|
Porcine |
Endothelial |
Trypsin: 0.25%
|
Medium 199 |
Endothelial Cells Grown On Permeable Membrane Supports,
J Tiss Cul Meth
14,
247, 1992 |
| Porcine
|
Pigs |
Endothelial |
Trypsin: 0.05%
|
DMEM |
Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells
,
Exp Cell Res
158,
15, 1985 |
| Porcine
|
Porcine |
Endothelial
Aortic |
Collagenase Type 2: 0.1%
|
Dulbecco-Vogt MEM w/o serum |
Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta
,
In Vitro 18,
63, 1982 |
| Porcine
|
Porcine, 30-40 kg |
Endothelia
Aortic |
Collagenase Type 4: 0.025%
|
Medium 199 |
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture
,
Atherosclerosis 39,
147, 1981 |
| Porcine
|
Porcine, 20-30 week |
Endothelial
Aortas
Veins |
Collagenase: 0.1%
|
Medium 199 w/BSS & HEPES or NaHCO3 |
The Porcine Endothelial Cell in Tissue Culture
,
Atherosclerosis 21,
259, 1975 |
| Porcine
|
Porcine, 60-100 days |
Aorta |
Trypsin: 0.1%
|
Phosphate buffer
(see reference) |
The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin
,
Biochim Biophys Acta
237,
378, 1971 |
| Rabbit
|
Rabbit |
Corneal endothelial cells (CEC) |
Hyaluronidase: 0.05%
|
DMEM |
Subcellular Localization of the Expressed 18 kDa FGF-2 Isoform in Corneal Endothelial Cells
,
Mol Vis
6,
222, 2000 |
| Rabbit
|
Rabbit, 2-3Kg |
Endothelial, aortic |
Elastase: 0.2%
|
Hanks solution |
Characterization of lipid-laden aortic cells from cholesterol-fed rabbits. I. Resolution of aortic cell populations by metrizamide density gradient centrifugation
,
Lab Invest 37,
287, 1977 |
| Rat
|
Rat, SD, male, 250-300 g |
Smooth muscle, aorta |
Soybean Trypsin Inhibitor: 0.25%
|
HBSS with 0.2 mM Ca++ |
Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells
,
J Biol Chem
263,
13791, 1988 |
| Rat
|
Rat, SD, male, 350 - 450 g |
Lipocytes
Kupffer
Sinusoidal endothelial |
Collagenase: 0.015%
|
DMEM/Ham's F-12 |
Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan
,
Anal Biochem
161,
207, 1987 |
| Rat
|
Rat, Wistar, male, 3 mo |
Endothelial
Kupffer
Parenchymal |
Pronase: 0.25%
|
HBSS |
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells.
,
J Biol Chem
258,
12221, 1983 |
| Rat
|
Rat, Wistar-Kyoto, male, 100 - 200 g |
Endothelial
Cerebral |
Collagenase Type 2: 0.05%
|
HBSS |
Primary Culture of Rat Cerebral Microvascular Endothelial Cells.
,
Lab Invest 46,
554, 1982 |
| Rat
|
Rat, 300 G, and pig, 30-40 Kg |
Endothelial
Thoracic aorta |
Trypsin: 0.05%
|
Medium 199 and 0.01M EDTA |
Interaction of aortic endothelial and smooth muscle cells in culture. Effect on glycosaminoglycan levels.
,
Atherosclerosis 39,
147, 1981 |
| Rat
|
Rat |
Foreskin |
Collagenase: 0.5%
|
Dulbecco's modified Eagle's medium w/10% calf serum |
Long-term Culture of Capillary Endothlial Cells
,
Proc Natl Acad Sci U S A 76,
5217, 1979 |
| Rat
|
Rat, Wistar, adult, 170 g |
Endothelial |
Trypsin: 0.5%
|
BSS |
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter
,
J Anat 129,
261, 1979 |