Endothelial - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine, (Bos taurus), calf Endothelial Pulmonary Collagenase: 1000 u/ml
 PBS, CMF Culture and Characterization of Pulmonary Microvascular Endothelial Cell , In Vitro Cell Dev Biol 28A, 711, 1992
Bovine Calf Endothelial Trypsin: 0.25%
 HEPES Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension , In Vitro Cell Dev Biol 28A, 403, 1992
Bovine Bovine, adult Cerebral artery Endothelial Collagenase: 0.2%
 HBSS Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries , In Vitro Cell Dev Biol 26, 291, 1990
Bovine Bovine Endothelial Aortic Trypsin: 0.05%
 Krebs Ringer solution Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled , Proc Natl Acad Sci U S A 85, 2334, 1988
Bovine Bovine Endothelial, pulmonary artery Collagenase: 0.1%
 CMF-Dulbecco's PBS Formation of Diacylglycerol by a Phospholipase D-phosphatidate Phosphatase Pathway Specific for Phosphatidylcholine in Endothelial Cells , Biochim Biophys Acta 962, 282, 1988
Bovine Bovine Endothelial Aorta Collagenase Type 2: 0.1%
 PBS Microvascular Endothelium and Pericytes: High Yield, Low Passage Cultures , In Vitro Cell Dev Biol 22, 344, 1986
Bovine Bovine Endothelial Aortic Collagenase Type 2: 0.1%
 DMEM Modulation of Sulfated Proteoglycan Synthesis by Bovine Aortic Endothelial Cells During Migration , J Cell Biol 102, 679, 1986
Bovine Bovine Endothelial Brain arteries Collagenase Type 2: 0.2%
 Dulbecco's PBS Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain , In Vitro Cell Dev Biol 21, 172, 1985
Bovine Calf Endothelial Smooth muscle Collagenase: 0.75%
 DMEM Identification and Isolation of Endothelial Cells Based on Their Increased Uptake of Acetylated-Low Density Lipoprotein , J Cell Biol 99, 2034, 1984
Bovine Bovine Endothelial Corneal Trypsin: 0.2%
 PBS: DMEM Investigation of the Attachment of Bovine Corneal Endothelial Cells , Exp Cell Res 144, 472, 1983
Bovine Bovine, 2-3 weeks Endothelial Pulmonary artery Collagenase Type 1: 0.2%
 RPMI 1640 w/1% Fetal Bovine Serum Ultrastructural Changes in Bovine Pulmonary Artery Endothelial Cells Exposed to 80% O2 In Vitro , In Vitro 19, 714, 1983
Bovine Bovine Endothelial, corneal Trypsin: 0.05%
 0.01M Phosphate buffer with 0.02% EDTA, 0.9% NaCl (See reference) Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells , J Cell Physiol 117, 368, 1983
Bovine Bovine Endothelial Subclavian vein Collagenase Type 1: 0.10%
 PBS An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells. , In Vitro 18, 99, 1982
Bovine Bovine Aortic Pulmonary artery Collagenase Type 2: 0.10%
 PBS Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells. , In Vitro 17, 450, 1981
Bovine Calf, fetal, 4-9 months Endothelial Collagenase Type 1: 0.25%
 PBS Proliferative Characteristics of Clonal Endothelial Cell Strains , J Cell Physiol 107, 123, 1981
Bovine Bovine, adult Aorta Collagenase Type 1: 125 u/ml
 Dulbecco's PBS with calcium and magnesium Studies of Morphologically Atypical ("Sprouting") Cultures of Bovine Aortic Endothelial Cells. Growth Characteristics and Connective Tissue Protein Synthesis , J Cell Physiol 102, 183, 1980
Bovine Bovine, young Pulmonary artery Collagenase: 0.1%
 Medium 199 Methods for Microcarrier Culture of Bovine Pulmonary Artery Endothelial Cells Avoiding the Use of Enzymes , Tissue Cell 12, 619, 1980
Bovine Bovine Foreskin Collagenase: 0.5%
 Dulbecco's modified Eagle's medium w/10% calf serum Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
Bovine Bovine Saphenous Vein Aorta Collagenase: 0.01%
 PBS Comparison of Tissue-Cultured Bovine Endothelial Cells from Aorta and Saphenous Vein , In Vitro 14, 903, 1978
Bovine Bovine Endothelial Thoracic aorta Saphenous veins Collagenase Type 2: 0.1%
 PBS Selection and Characterization of Bovine Aortic Endothelial Cells , In Vitro 14, 966, 1978
Bovine Bovine Pulmonary artery Collagenase Type 2: 0.25%
 Puck's solution Isolation And Culture of Pulmonary Artery Endothelial Cells , Tissue Cell 10, 535, 1978
Bovine Calf Endothelial Collagenase Type 2: 0.1%
 DMEM Characterization of the Collagen Synthesized by Endothelial Cells in Culture , Proc Natl Acad Sci U S A 73 (7), 2361, 1976
Canine Dog
(also human)		 Microvessels Collagenase Type 4: 0.1%
 DMEM Cutlured Human and Canine Endothelial Cells from Brain Microvessels , Brain Res Bull 21, 785, 1988
Canine Dog, mongrel, adult Endothelium Jugular vein Trypsin: 0.1%
 Earle's PBS, CMF Isolation of Adult Canine Venous Endothelium for Tissue Culture , In Vitro 17, 44, 1981
Guinea-Pig guinea pig, female, 300-350g coronary endothelial Collagenase Type 2: 0.1%
 see reference Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium. , Am J Physiol Heart Circ Physiol 281, H1657-66, 2001
Human Human HUVEC Collagenase Type 4: 0.1%
 RPMI 1640 Neuropeptide Y expression, localization and cellular transducing effects in HUVEC , Biol Cell 97(6), 457, 2005
Human Human colonic endothelial cells Collagenase Type 2: 0.25%
 HBSS/5%FBS Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells , Am J Physiol/Gastro 282, G1088, 2002
Human human pulmonary vascular endothelial cells Dispase: 1.18 u/ml
Elastase: 10 u/ml
 M199 Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro. , Exp Mol Pathol 72, 221, 2002
Human Human Endothelial Trypsin: 2%
 PBS Comparsion of the Rates of Deamidation, Diketopiperazine Formation and Oxidation in Recombinant Human Vascular Endothelial Growth Factor and Model Peptides , AAPS PharmSci 2 (1), 5, 2000
Human Human HUVEC, porcine pulmonary arterial endothelial cells Collagenase Type 2: 0.2%
 DMEM Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells. , Circulation 101(19), 2317, 2000
Human Human hepatic endothelial cells Collagenase: 0.2%
 DMEM A simplified method for the isolation and culture of endothelial cells from pseudointima of transjugular intrahepatic portasystemic shunts. , Lab Invest 78(11), 1469, 1998
Human Human Endothelial Trypsin: 0.3.%
 HBSS
(see reference)		 VCAM-1-, ELAM-1-, and ICAM-1-Independent Adhesion of Melanoma Cells to Cultured Human Dermal Microvascular Endothelial Cells , J Invest Dermatol 98, 79, 1992
Human human vascular endothelial Dispase: 0.15%
Trypsin: 0.25%
 M-199 Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis. , Am J Physiol 262, H1088-95, 1992
Human Human Umbilical vein HUVE Collagenase: 0.1%
 Cord buffer
(see reference)		 Intracellular Mechanisms Involved in Basement Membrane Induced Blood Vessel Differentiation In Vitro , In Vitro Cell Dev Biol 27, 327, 1991
Human Human Crypt cells Collagenase: 125 u/ml
 RMPI 1640 A Method For the Prolonged Culture of Colonic Epithelial Cells, J Tiss Cul Meth 13, 103, 1991
Human Human Endothelial Collagenase Type 1: 0.2%
 Medium 199 Migration Assay for Endothelial Cells in Multiwells Application to Studies on the Effect of Opioids , J Immunol Methods 128, 235, 1990
Human Human Human umbilical vein endothelial cells Collagenase Type 2: 75 u/ml
 M199 A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions. , J Exp Med 170, 399-414, 1989
Human Human Microvessels Collagenase Type 4: 0.1%
 DMEM Cutlured Human and Canine Endothelial Cells from Brain Microvessels , Brain Res Bull 21, 785, 1988
Human Human Endothelial/HUVE Foreskin & umbilical cord Trypsin: 0.3%
 HBSS/PBS, Medium 199
(see reference)		 Role of Laminin and Basement Membrane in Morphological Differentiation of Human Endothelial Cells into Capillary-like Structures , J Cell Biol 107, 1589, 1988
Human Human Umbilical cord Smooth muscle Collagenase: 0.1%
 HEPES Comparative Endocrinology-Paracrinology-Autocrinology of Human Adult Large Vessel Endothelial and Smooth Muscle Cells , In Vitro Cell Dev Biol 24 (4), 309, 1988
Human Human Endothelial Saphenous vein Collagenase Type 2: 0.1%
 PBS, CMF The Cytoxic Effect of Surgical Glove Powder Particles on Adult Human Vascular Endothelial Cell Cultures: Implications for Clinical Uses of Tissue Culture Techniques , J Surg Res 41, 463, 1986
Human Human Endothelial Collagenase: 0.1%
 HEPES Isolation, Growth Requirements, Cloning, Prostacylclin Production and Life-Span of Human Adult Endothelial Cells in Low Serum Culture Medium , In Vitro Cell Dev Biol 22 (1), 51, 1986
Human Human Endothelial Dermal Trypsin: 0.3%
 PBS Human Dermal Microvascular Endothelial Cells: An Improved Method for Tissue Culture and Description of Some Singular Properties in Culture , In Vitro Cell Dev Biol 21, 627, 1985
Human Human Fibroblasts Foreskin Hyaluronidase: 0.10%
 DMEM Long-Term Culture of Human Endothelial Cells , In Vitro 19, 661, 1983
Human Human Iliac arteries Collagenase: 0.25%
 PBS w/Ca++, Mg++, & BSA
(see reference)		 Cultured Endothelial Cells Derived from the Human Iliac Arteries , In Vitro 18, 859, 1982
Human Human, adult Peripheral blood mononuclear Monocytes T cells Endothelial Collagenase: 0.25%
 RPMI 1640 Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro. , J Clin Invest 67, 1490, 1981
Human Human Microvascular endothelial Neonatal foreskins Dispase: at 1000 u/ml
 Konigsberg's modification of HBSS
(see reference)		 Cultivation of Microvascular Endothelial Cells from Human Preputial Skin , In Vitro 16, 675, 1980
Human Human (adult and child) Foreskin Collagenase: 0.5%
 Dulbecco's modified Eagle's medium w/10% calf serum Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
Human Human Umbilical vein Trypsin: 100 µg/ml
 Tris-HCl,0.2 M Synthesis of Basement Membrane Collagen By Cultured Human Endothelial Cells , J Exp Med 144, 209, 1976
Human Human Umbilical vein Collagenase Type 1: 125 u/ml
 Dulbecco's PBS Culture of Vascular Endothelium , Progress in Hemostasis and Thrombosis Vol. 3,(Spaet,T.H.,ed.), Grune and Stratton, NY, 1, 1976
Human Human Umbilical cord Collagenase: 0.1%
 Dulbecco's PBS Human Vascular Endothelial Cells in Culture: Growth and DNA Synthesis , J Cell Biol 60, 673, 1974
Human Human Umbilical cord Collagenase: 0.2%
 Cord buffer
(see reference)		 Culture of Human Endothelial Cells Derived from Umbilical Veins. Identification by Morphologic and Immunologic Criteria , J Clin Invest 52, 2745, 1973
Human Human Umbilical cord Trypsin NF 1:250: 0.125%
 Saline, normal Replication of Human Endothelial Cells in Culture , Science 181, 452, 1973
Human Human Umbilical cord Trypsin NF 1:250: 0.25%
 CMF solution Human Endothelium in Cell Culture , J Atheroscler Res 6, 151, 1966
Mouse mouse, embryonic or yolk sac endothelial Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 0.001%
 PBS Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation. , J Cell Biol 172, 151-62, 2006
Mouse mouse, 4 week retinal endothelial cells Collagenase Type 1: 0.1%
 DMEM Isolation and characterization of murine retinal endothelial cells. , Mol Vis 9, 171, 2003
Mouse mouse, male endothelial cells from lymph node Collagenase Type 1: 0.1%
 PBS Expression profile of active genes in mouse lymph node high endothelial cells. , Int Immunol 11(12), 1989, 1999
Porcine Porcine
(also bovine)		 Endothelial Collagenase Type 1:
DMEM w/ 10% calf serum Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury , J Surg Res 99, 228, 2001
Porcine porcine HUVEC, porcine pulmonary arterial endothelial cells Collagenase Type 2: 0.2%
 DMEM Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells. , Circulation 101(19), 2317, 2000
Porcine pig, 6-7 month porcine pulmonary endothelial Collagenase Type 1: 0.3%
 RPMI 1640 Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV. , J Am Soc Nephrol 10(3), 481, 1999
Porcine Porcine Endothelial Trypsin: 0.25%
 Medium 199 Endothelial Cells Grown On Permeable Membrane Supports, J Tiss Cul Meth 14, 247, 1992
Porcine Pigs Endothelial Trypsin: 0.05%
 DMEM Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells , Exp Cell Res 158, 15, 1985
Porcine Porcine Endothelial Aortic Collagenase Type 2: 0.1%
 Dulbecco-Vogt MEM w/o serum Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta , In Vitro 18, 63, 1982
Porcine Porcine, 30-40 kg Endothelia Aortic Collagenase Type 4: 0.025%
 Medium 199 Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture , Atherosclerosis 39, 147, 1981
Porcine Porcine, 20-30 week Endothelial Aortas Veins Collagenase: 0.1%
 Medium 199 w/BSS & HEPES or NaHCO3 The Porcine Endothelial Cell in Tissue Culture , Atherosclerosis 21, 259, 1975
Porcine Porcine, 60-100 days Aorta Trypsin: 0.1%
 Phosphate buffer
(see reference)		 The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin , Biochim Biophys Acta 237, 378, 1971
Rabbit Rabbit Corneal endothelial cells (CEC) Hyaluronidase: 0.05%
 DMEM Subcellular Localization of the Expressed 18 kDa FGF-2 Isoform in Corneal Endothelial Cells , Mol Vis 6, 222, 2000
Rabbit Rabbit, 2-3Kg Endothelial, aortic Elastase: 0.2%
 Hanks solution Characterization of lipid-laden aortic cells from cholesterol-fed rabbits. I. Resolution of aortic cell populations by metrizamide density gradient centrifugation , Lab Invest 37, 287, 1977
Rat Rat, SD, male, 250-300 g Smooth muscle, aorta Soybean Trypsin Inhibitor: 0.25%
 HBSS with 0.2 mM Ca++ Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells , J Biol Chem 263, 13791, 1988
Rat Rat, SD, male, 350 - 450 g Lipocytes Kupffer Sinusoidal endothelial Collagenase: 0.015%
 DMEM/Ham's F-12 Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan , Anal Biochem 161, 207, 1987
Rat Rat, Wistar, male, 3 mo Endothelial Kupffer Parenchymal Pronase: 0.25%
 HBSS In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells. , J Biol Chem 258, 12221, 1983
Rat Rat, Wistar-Kyoto, male, 100 - 200 g Endothelial Cerebral Collagenase Type 2: 0.05%
 HBSS Primary Culture of Rat Cerebral Microvascular Endothelial Cells. , Lab Invest 46, 554, 1982
Rat Rat, 300 G, and pig, 30-40 Kg Endothelial Thoracic aorta Trypsin: 0.05%
 Medium 199 and 0.01M EDTA Interaction of aortic endothelial and smooth muscle cells in culture. Effect on glycosaminoglycan levels. , Atherosclerosis 39, 147, 1981
Rat Rat Foreskin Collagenase: 0.5%
 Dulbecco's modified Eagle's medium w/10% calf serum Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
Rat Rat, Wistar, adult, 170 g Endothelial Trypsin: 0.5%
 BSS Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter , J Anat 129, 261, 1979