Endothelial - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Bovine umbilical cord (BUVEC) Collagenase: 0.1%
 Dulbecco's/Ham F-12 Ricken Albert M, Traenkner Anja, Merkwitz Claudia, Hummitzsch Katja, Grosche Jens, Spanel-Borowski Katharina: The short prolactin receptor predominates in endothelial cells of micro- and macrovascular origin, J. Vasc. Res. 44, 19-30, 2007
Bovine Bovine, (Bos taurus), calf Endothelial
Pulmonary		 Collagenase: 1000 u/ml
 PBS, CMF Del Vecchio, P.J., Siflinger-Birnboim, A., Belloni, P.N., Holleron, L.A., Lum, H., and Malik, A.B.: Culture and Characterization of Pulmonary Microvascular Endothelial Cell, In Vitro Cell. Dev. Biol. 28A, 711, 1992
Bovine Calf Endothelial Trypsin: 0.25%
 HEPES Vender, R.: Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension, In Vitro Cell. Dev. Biol. 28A, 403, 1992
Bovine Bovine, adult Cerebral artery
Endothelial		 Collagenase: 0.2%
 HBSS Machi, T., Kassell, N.F., and Scheld, W.M.: Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries, In Vitro Cell. Dev. Biol. 26, 291, 1990
Bovine Bovine Endothelial
Aortic		 Trypsin: 0.05%
 Krebs Ringer solution DeNucci, G., Gryglewski, R.J., Warner, T.D., and Vane, J.R.: Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled, Proc. Natl. Acad. Sci. USA 85, 2334, 1988
Bovine Bovine Endothelial, pulmonary artery Collagenase: 0.1%
 CMF-Dulbecco's PBS Martin, T.: Formation of Diacylglycerol by a Phospholipase D-phosphatidate Phosphatase Pathway Specific for Phosphatidylcholine in Endothelial Cells, Biochim. Biophys. Acta 962, 282, 1988
Bovine Bovine Endothelial
Aorta		 Collagenase Type 2: 0.1%
 PBS Carson, M.P. and Haudenschild, C.C.: Microvascular Endothelium and Pericytes: High Yield, Low Passage Cultures, In Vitro Cell. Dev. Biol. 22, 344, 1986
Bovine Bovine Endothelial
Aortic		 Collagenase Type 2: 0.1%
 DMEM Kinsella, Michael G. and Wight, Thomas N.: Modulation of Sulfated Proteoglycan Synthesis by Bovine Aortic Endothelial Cells During Migration, J. Cell Biol. 102, 679, 1986
Bovine Bovine Endothelial Collagenase: 0.5%
 DMEM/Ham's F-12 Gospodarowicz, D., Massoglia, S., Cheng, J., and Fujii, D.: Effect of Fibroblast Growth Factor and Lipoproteins on the Proliferation of Endothelial Cells Derived From Bovine Adrenal Cortex, Brain Cortex, and Corpus Luteum Capillaries, J. Cell. Physiol. 127, 121, 1986
Bovine Bovine Endothelial
Brain arteries		 Collagenase Type 2: 0.2%
 Dulbecco's PBS Goetz, I.., Warren, J., Estrada, C., Roberts, E., and Krause, D.: Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain, In Vitro Cell. Dev. Biol. 21, 172, 1985
Bovine Calf Endothelial
Smooth muscle		 Collagenase: 0.75%
 DMEM Voyta, J., Via, D., Butterfield, C., and Zetter, B.: Identification and Isolation of Endothelial Cells Based on Their Increased Uptake of Acetylated-Low Density Lipoprotein, J. Cell Biol. 99, 2034, 1984
Bovine Bovine Endothelial
Corneal		 Trypsin: 0.2%
 PBS: DMEM Scott, D., Murray, J., and Barnes, M.: Investigation of the Attachment of Bovine Corneal Endothelial Cells, Exp. Cell Res. 144, 472, 1983
Bovine Bovine, 2-3 weeks Endothelial
Pulmonary artery		 Collagenase Type 1: 0.2%
 RPMI 1640 w/1% Fetal Bovine Serum Lee, S., Douglas, W., Deneke, S., and Fanburg, B.: Ultrastructural Changes in Bovine Pulmonary Artery Endothelial Cells Exposed to 80% O2 In Vitro, In Vitro 19, 714, 1983
Bovine Bovine Endothelial, corneal Trypsin: 0.05%
 0.01M Phosphate buffer with 0.02% EDTA, 0.9% NaCl (See reference) Robinson, J. and Gospodarowicz, D.: Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells, J. Cell. Physiol. 117, 368, 1983
Bovine Bovine Endothelial
Subclavian vein		 Collagenase Type 1: 0.10%
 PBS Olander, J., Marasa, J., Kimes, R., Johnston, G., and Feder, J.: An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells, In Vitro 18, 99, 1982
Bovine Bovine Aortic
Pulmonary artery		 Collagenase Type 2: 0.10%
 PBS Makarski, J. S.: Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells, In Vitro 17, 450, 1981
Bovine Calf, fetal, 4-9 months Endothelial Collagenase Type 1: 0.25%
 PBS Rosen, E., Mueller, S., Noveral, J., and Levine, E.: Proliferative Characteristics of Clonal Endothelial Cell Strains, J. Cell. Physiol. 107, 123, 1981
Bovine Bovine, adult Aorta Collagenase Type 1: 125 u/ml
 Dulbecco's PBS with calcium and magnesium Cotta-Pereira, G., Sage, H., Bornstein, P., Ross, R., and Schwartz, S.: Studies of Morphologically Atypical ("Sprouting") Cultures of Bovine Aortic Endothelial Cells. Growth Characteristics and Connective Tissue Protein Synthesis, J. Cell. Physiol. 102, 183, 1980
Bovine Bovine, young Pulmonary artery Collagenase: 0.1%
 Medium 199 Ryan, U., Mortara, M., and Whitaker, C.: Methods for Microcarrier Culture of Bovine Pulmonary Artery Endothelial Cells Avoiding the Use of Enzymes, Tissue Cell 12, 619, 1980
Bovine Bovine Foreskin Collagenase: 0.5%
 Dulbecco's MEM w/10% calf serum Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc. Natl. Acad. Sci. USA 76, 5217, 1979
Bovine Bovine Saphenous Vein
Aorta		 Collagenase: 0.01%
 PBS Eskin, S., Sybers, H., Trevino, L., Lie, J., and Chimoskey, J.: Comparison of Tissue-Cultured Bovine Endothelial Cells from Aorta and Saphenous Vein, In Vitro 14, 903, 1978
Bovine Bovine Endothelial
Thoracic aorta
Saphenous veins		 Collagenase Type 2: 0.1%
 PBS Schwartz, S.M.: Selection and Characterization of Bovine Aortic Endothelial Cells, In Vitro 14, 966, 1978
Bovine Bovine Pulmonary artery Collagenase Type 2: 0.25%
 Puck's solution Ryan, U.S., Clements, E., Habliston, D., and Ryan, J.W.: Isolation And Culture of Pulmonary Artery Endothelial Cells, Tissue Cell 10, 535, 1978
Bovine Calf Endothelial Collagenase Type 2: 0.1%
 DMEM Howard, B., Macarak, E., Gunson, D., and Kefalides, N.: Characterization of the Collagen Synthesized by Endothelial Cells in Culture, Proc. Natl. Acad. Sci. USA 73 (7), 2361, 1976
Canine Dog
(also human)		 Microvessels Collagenase Type 4: 0.1%
 DMEM Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels, Brain Res. Bull. 21, 785, 1988
Canine Dog, mongrel, adult Endothelium
Jugular vein		 Trypsin: 0.1%
 Earle's PBS, CMF Ford, J., Burkel, W., and Kahn, R.: Isolation of Adult Canine Venous Endothelium for Tissue Culture, In Vitro 17, 44, 1981
Guinea-Pig Guinea pig, female, 300-350g Coronary endothelial Collagenase Type 2: 0.1%
 see reference Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J: Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium, Am. J. Physiol. Heart Circ. Physiol. 281, H1657-66, 2001
Human Human, 18-68 yr Corneal endothelial Collagenase: 0.2%
Neutral Protease: 1.0%
 DMEM/F12 Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G: A novel method of isolation, preservation, and expansion of human corneal endothelial cells, Inv. Ophthal. Visual Sci. 48, 614-20, 2007
Human Human HUVEC Collagenase Type 4: 0.1%
 RPMI 1640 Silva AP, Kaufmann JE, Vivancos C, Fakan S, Cavadas C, Shaw P, Brunner HR, Vischer U, and Grouzmann E.: Neuropeptide Y expression, localization and cellular transducing effects in HUVEC, Biol. Cell. 97(6), 457, 2005
Human Human Colonic endothelial cells Collagenase Type 2: 0.25%
 HBSS/5%FBS Wang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells, Am. J. Physiol. / Gastro. 282, G1088, 2002
Human Human Pulmonary vascular endothelial cells Neutral Protease: 1.18 u/ml
Elastase: 10 u/ml
 M199 Muller AM, Hermanns MI, Skrzynski C, Nesslinger M, Muller KM, and Kirkpatrick CJ.: Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro, Exp. Mol. Pathol. 72, 221, 2002
Human Human HUVEC Collagenase Type 2: 0.1%
 PBS Takano Manabu, Meneshian Avedis, Sheikh Emran, Yamakawa Yasuhiko, Wilkins Kirsten Bass, Hopkins Elise A, Bulkley Gregory B: Rapid upregulation of endothelial P-selectin expression via reactive oxygen species generation, Am. J. Physiol. Heart Circ. Physiol. 283, H2054-61, 2002
Human Human Endothelial Trypsin: 2%
 PBS Goolcharran, C., Cleland, J., Keck, R., Jones, A., and Borchardt, R.: Comparsion of the Rates of Deamidation, Diketopiperazine Formation and Oxidation in Recombinant Human Vascular Endothelial Growth Factor and Model Peptides, A.A.P.S. PharmSci 2 (1), 5, 2000
Human Human HUVEC, porcine pulmonary arterial endothelial cells Collagenase Type 2: 0.2%
 DMEM Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY, and Koh GY.: Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells, Circulation 101(19), 2317, 2000
Human Human Vascular endothelial cells Collagenase: 0.1%
 DMEM Schonbeck U, Sukhova GK, Graber P, Coulter S, Libby P: Augmented expression of cyclooxygenase-2 in human atherosclerotic lesions, Am. J. Pathol. 155, 1281-91, 1999
Human Human Hepatic endothelial cells Collagenase: 0.2%
 DMEM Sanyal AJ, and Mirshahi F.: A simplified method for the isolation and culture of endothelial cells from pseudointima of transjugular intrahepatic portasystemic shunts, Lab. Invest. 78(11), 1469, 1998
Human Human Foreskin microvascular endothelial Trypsin: 0.3%
 HBSS Wojta J, Gallicchio M, Zoellner H, Filonzi E, Hamilton J, McGrath K: Interleukin-4 stimulates expression of urokinase-type-plasminogen activator in cultured human foreskin microvascular endothelial cells, Blood 81, 3285-92, 1993
Human Human Endothelial Trypsin: 0.3.%
 HBSS
(see reference)		 Lee, K, Lawley, T, Xu, Y, and Swerlick, R.: VCAM-1-, ELAM-1-, and ICAM-1-Independent Adhesion of Melanoma Cells to Cultured Human Dermal Microvascular Endothelial Cells, J. Invest. Dermatol. 98, 79, 1992
Human Human Vascular endothelial Neutral Protease: 0.15%
Trypsin: 0.25%
 M-199 Farber HW, Antonov AS, Romanov YA, Smirnov VN, Scarfo LM, Beer DJ: Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis, Am. J. Physiol. 262, H1088-95, 1992
Human Human Umbilical vein
HUVE		 Collagenase: 0.1%
 Cord buffer
(see reference)		 Grant, D.S., Lelkes, P.I., Fukuda, K., Kleinman, H.K.: Intracellular Mechanisms Involved in Basement Membrane Induced Blood Vessel Differentiation In Vitro, In Vitro Cell. Dev. Biol. 27, 327, 1991
Human Human Crypt cells Collagenase: 125 u/ml
 RMPI 1640 Whitehead, R., and Eeden, P.: A Method For the Prolonged Culture of Colonic Epithelial Cells, J. Tiss. Cult. Meth. 13, 103, 1991
Human Human Endothelial Collagenase Type 1: 0.2%
 Medium 199 Fischer, E., Stingl, A., and Kirkpatrick, C.: Migration Assay for Endothelial Cells in Multiwells Application to Studies on the Effect of Opioids, J. Immunol. Methods 128, 235, 1990
Human Human Human umbilical vein endothelial cells Collagenase Type 2: 75 u/ml
 M199 Muller WA, Ratti CM, McDonnell SL, Cohn ZA: A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions, J. Exp. Med. 170, 399-414, 1989
Human Human Microvessels Collagenase Type 4: 0.1%
 DMEM Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels, Brain Res. Bull. 21, 785, 1988
Human Human Endothelial/HUVE
Foreskin & umbilical cord		 Trypsin: 0.3%
 HBSS/PBS, Medium 199
(see reference)		 Kubota, Y., Kleinman, H., Martin, G., and Lawley, T.: Role of Laminin and Basement Membrane in Morphological Differentiation of Human Endothelial Cells into Capillary-like Structures, J. Cell Biol. 107, 1589, 1988
Human Human Umbilical cord
Smooth muscle		 Collagenase: 0.1%
 HEPES Hoshi, H., Kan, M., Chen, J., and McKeehan, W.: Comparative Endocrinology-Paracrinology-Autocrinology of Human Adult Large Vessel Endothelial and Smooth Muscle Cells, In Vitro Cell. Dev. Biol. 24 (4), 309, 1988
Human Human Endothelial
Saphenous vein		 Collagenase Type 2: 0.1%
 PBS, CMF Sharefkin, J.B., Fairchild, K.D., Albus, R.A., Cruess, D.F., and Rich, N.M.: The Cytoxic Effect of Surgical Glove Powder Particles on Adult Human Vascular Endothelial Cell Cultures: Implications for Clinical Uses of Tissue Culture Techniques, J. Surg. Res. 41, 463, 1986
Human Human Endothelial Collagenase: 0.1%
 HEPES Hoshi, H., and McKeehan, W.: Isolation, Growth Requirements, Cloning, Prostacylclin Production and Life-Span of Human Adult Endothelial Cells in Low Serum Culture Medium, In Vitro Cell. Dev. Biol. 22 (1), 51, 1986
Human Human Endothelial
Dermal		 Trypsin: 0.3%
 PBS Marks, R.M., Czerniecki, M., and Penny, R.: Human Dermal Microvascular Endothelial Cells: An Improved Method for Tissue Culture and Description of Some Singular Properties in Culture, In Vitro Cell. Dev. Biol. 21, 627, 1985
Human Human Fibroblasts
Foreskin		 Hyaluronidase: 0.10%
 DMEM Gordon, P., Sussman, I., and Hatcher, V.: Long-Term Culture of Human Endothelial Cells, In Vitro 19, 661, 1983
Human Human Iliac arteries Collagenase: 0.25%
 PBS w/Ca++, Mg++, & BSA
(see reference)		 Glassberg, M., Bern, M., Coughlin, S., Haudenschild, C., Hoyer, L., and Antoniades, H.: Cultured Endothelial Cells Derived from the Human Iliac Arteries, In Vitro 18, 859, 1982
Human Human, adult Peripheral blood mononuclear
Monocytes
T cells
Endothelial		 Collagenase: 0.25%
 RPMI 1640 Ashida, E., Johnson, A., and Lipsky, P.: Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro, J. Clin. Invest. 67, 1490, 1981
Human Human Microvascular endothelial
Neonatal foreskins		 Neutral Protease: at 1000 u/ml
 Konigsberg's modification of HBSS
(see reference)		 Sherer, G., Fitzharris, T., Faulk, W., and LeRoy, E.: Cultivation of Microvascular Endothelial Cells from Human Preputial Skin, In Vitro 16, 675, 1980
Human Human (adult and child) Foreskin Collagenase: 0.5%
 Dulbecco's MEM w/10% calf serum Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc. Natl. Acad. Sci. USA 76, 5217, 1979
Human Human Umbilical vein
 Trypsin: 100 µg/ml
 Tris-HCl,0.2 M Jaffe, E.A., Minick, C.R., Adelman, B., Becker, C.G., and Nachman, R.: Synthesis of Basement Membrane Collagen By Cultured Human Endothelial Cells, J. Exp. Med. 144, 209, 1976
Human Human Umbilical vein Collagenase Type 1: 125 u/ml
 Dulbecco's PBS Gimbrone Jr., M.A.: Culture of Vascular Endothelium, Prog. Hemost. Thromb. 3, 1, 1976
Human Human Umbilical cord Collagenase: 0.1%
 Dulbecco's PBS Gimbrone, M.A., Cotran, R.S., and Folkman, J.: Human Vascular Endothelial Cells in Culture: Growth and DNA Synthesis, J. Cell Biol. 60, 673, 1974
Human Human Umbilical cord Collagenase: 0.2%
 Cord buffer
(see reference)		 Jaffe, E., Nachman, R., Becker, C., and Minick, C.: Culture of Human Endothelial Cells Derived from Umbilical Veins. Identification by Morphologic and Immunologic Criteria, J. Clin. Invest. 52, 2745, 1973
Human Human Umbilical cord Trypsin NF 1:250: 0.125%
 Saline, normal Lewis, L.J., Haok, J.C., Maca, R.D., and Fry, G.L.: Replication of Human Endothelial Cells in Culture, Science 181, 452, 1973
Human Human Umbilical cord Trypsin NF 1:250: 0.25%
 CMF solution Fryer, D.G., Birnbaum, G., and Luttrell, C.N.: Human Endothelium in Cell Culture, J. Atheroscler. Res. 6, 151, 1966
Mouse Mouse, embryonic or yolk sac Endothelial Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 0.001%
 PBS Braren Rickmer, Hu Huiqing, Kim YungHae, Beggs HilaryE, Reichardt LouisF, Wang Rong: Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation, J. Cell Biol. 172, 151-62, 2006
Mouse Mouse, 4 week Retinal endothelial cells Collagenase Type 1: 0.1%
 DMEM Su X, Sorenson CM, and Sheibani N.: Isolation and characterization of murine retinal endothelial cells, Mol. Vis. 9, 171, 2003
Mouse Mouse, male Endothelial cells from lymph node Collagenase Type 1: 0.1%
 PBS Izawa D, Tanaka T, Saito K, Ogihara H, Usui T, Kawamoto S, Matsubara K, Okubo K, and Miyasaka M: Expression profile of active genes in mouse lymph node high endothelial cells, Int. Immunol. 11(12), 1989, 1999
Mouse Mouse, 2-4 week cerebrovascular endothelial Collagenase/Dispase: 0.1%
 PBS Sapatino, B., Welsh, C., Smith, C., Bebo, B., and Linticum, D.: Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme, In Vitro Cell. Dev. Biol. 29A, 923, 1993
Porcine Porcine
(also bovine)		 Endothelial Collagenase Type 1:
DMEM w/ 10% calf serum Nugent, H., and Edelman, E.: Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury, J. Surg. Res. 99, 228, 2001
Porcine Porcine HUVEC, porcine pulmonary arterial endothelial cells Collagenase Type 2: 0.2%
 DMEM Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY, and Koh GY.: Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells, Circulation 101(19), 2317, 2000
Porcine Porcine, 6-7 month Porcine pulmonary endothelial Collagenase Type 1: 0.3%
 RPMI 1640 Hill-Kapturczak N, Kapturczak MH, Block ER, Patel JM, Malinski T, Madsen KM, and Tisher CC.: Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV, J. Am. Soc. Nephrol. 10(3), 481, 1999
Porcine Porcine Endothelial Trypsin: 0.25%
 Medium 199 Shasby, S.: Endothelial Cells Grown On Permeable Membrane Supports, J. Tiss. Cult. Meth. 14, 247, 1992
Porcine Porcine Endothelial Trypsin: 0.05%
 DMEM Vischer, P., and Buddecke, E.: Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells, Exp. Cell Res. 158, 15, 1985
Porcine Porcine Endothelial
Aortic		 Collagenase Type 2: 0.1%
 Dulbecco-Vogt MEM w/o serum Dickinson, E. and Slakey, L.: Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta, In Vitro 18, 63, 1982
Porcine Porcine, 30-40 kg Endothelia
Aortic		 Collagenase Type 4: 0.025%
 Medium 199 Merrilees, M.J., and Scott, L.: Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture, Atherosclerosis 39, 147, 1981
Porcine Porcine, 20-30 week Endothelial
Aortas
Veins		 Collagenase: 0.1%
 Medium 199 w/BSS & HEPES or NaHCO3 Slater, D.N., and Sloan, J.M.: The Porcine Endothelial Cell in Tissue Culture, Atherosclerosis 21, 259, 1975
Porcine Porcine, 60-100 days Aorta Trypsin: 0.1%
 Phosphate buffer
(see reference)		 Coulson, W.F.: The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin, Biochim. Biophys. Acta 237, 378, 1971
Rabbit Rabbit Corneal endothelial cells (CEC) Hyaluronidase: 0.05%
 DMEM Choi, J., Ko, M., and Kay, E.: Subcellular Localization of the Expressed 18 kDa FGF-2 Isoform in Corneal Endothelial Cells, Mol. Vis. 6, 222, 2000
Rabbit Rabbit, 2-3Kg Endothelial, aortic Elastase: 0.2%
 Hanks solution Haley, N., Shio, H., Fowler, S.: Characterization of lipid-laden aortic cells from cholesterol-fed rabbits. I. Resolution of aortic cell populations by metrizamide density gradient centrifugation, Lab. Invest. 37, 287, 1977
Rat Rat, SD, male, 250-300 g Smooth muscle, aorta Soybean Trypsin Inhibitor: 0.25%
 HBSS with 0.2 mM Ca++ Schwertschlag, U.S., and Whorton, A.R.: Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells, J. Biol. Chem. 263, 13791, 1988
Rat Rat, SD, male, 350 - 450 g Lipocytes
Kupffer
Sinusoidal endothelial		 Collagenase: 0.015%
 DMEM/Ham's F-12 Friedman, S. and Roll, F.: Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan, Anal. Biochem. 161, 207, 1987
Rat Rat, Wistar, male, 3 mo Endothelial
Kupffer
Parenchymal		 Pronase: 0.25%
 HBSS Nagelkenke, J., Barto, K., and VanBerkel, T.: In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells, J. Biol. Chem. 258, 12221, 1983
Rat Rat, Wistar-Kyoto, male, 100 - 200 g Endothelial
Cerebral		 Collagenase Type 2: 0.05%
 HBSS Diglio, C.A., Grammas, P., Filiberto Giacomelli, M.S., and Wiener, J.: Primary Culture of Rat Cerebral Microvascular Endothelial Cells, Lab. Invest. 46, 554, 1982
Rat Rat, 300 G, and pig, 30-40 Kg Endothelial
Thoracic aorta		 Trypsin: 0.05%
 Medium 199 and 0.01M EDTA Merrilees, M.J., and Scott, L.: Interaction of aortic endothelial and smooth muscle cells in culture. Effect on glycosaminoglycan levels, Atherosclerosis 39, 147, 1981
Rat Rat Foreskin Collagenase: 0.5%
 Dulbecco's MEM w/10% calf serum Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc. Natl. Acad. Sci. USA 76, 5217, 1979
Rat Rat, Wistar, adult, 170 g Endothelial Trypsin: 0.5%
 BSS Phillips, P., Kumar, P., Kumar, S., and Waghe, M.: Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter, J. Anat. 129, 261, 1979