Colon - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Human Human Cancer stem cell Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
 RPMI-1640 Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells, Proc Natl Acad Sci U S A 104, 10158-63, 2007
Human Human colonic epithelial Collagenase:
Neutral Protease: 0.3%
Deoxyribonuclease I: 0.05%
 RPMI 1640 Fukushima Kouhei, Fiocchi Claudio: Paradoxical decrease of mitochondrial DNA deletions in epithelial cells of active ulcerative colitis patients.,Am J Physiol Gastrointest Liver Physiol Vol. 286,, , G804-13, 2004
Human Human Colonic endothelial cells Collagenase Type 2: 0.25%
 HBSS/5%FBS Wang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells, Am J Physiol/Gastro 282, G1088, 2002
Human Human Colonocytes Collagenase:
DMEM/F12 Emenaker N, Calaf G, Cox D, Basson M and Qureshi N: Short chain fatty acids differentially modulate cellular phenotype and c-myc protein levels in primary human nonmalignant and malignant colonocytes, J Nutr 46, 96-105, 2001
Human Human epithelial and mucosal lymphocytes Neutral Protease: 0.1%
CLSPA: 0.02%
Deoxyribonuclease I: 0.01%
 RPMI 1640 Hisamatsu T, Watanabe M, Ogata H, Ezaki T, Hozawa S, Ishii H, Kanai T, Hibi T: Interferon-inducible gene family 1-8U expression in colitis-associated colon cancer and severely inflamed mucosa in ulcerative colitis., Cancer Res 59, 5927-31, 1999
Human Human colonic epithelial Neutral Protease: 1.2 u/ml
Collagenase Type 4: 50 u/ml
 HBSS Gibson PR, Rosella O, Wilson AJ, Mariadason JM, Rickard K, Byron K, Barkla DH: Colonic epithelial cell activation and the paradoxical effects of butyrate., Carcinogenesis 20, 539-44, 1999
Human Human Lamina propria lymphocytes Collagenase: 25 u/ml
 HBSS Ueyama H, Kiyohara T, Sawada N, Isozaki K, Kitamura S, Kondo S, Miyagawa J, Kanayama S, Shinomura Y, Ishikawa H, Ohtani T, Nezu R, Nagata S, Matsuzawa Y: High Fas ligand expression on lymphocytes in lesions of ulcerative colitis, Gut 43, 48-55, 1998
Mouse Mouse, 6-8 week Cancer stem cell Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
 RPMI-1640 Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells, Proc Natl Acad Sci U S A 104, 10158-63, 2007
Mouse Mouse Lamina propria mononuclear cells Collagenase: 0.05%
Deoxyribonuclease I: 0.05%
Neutral Protease: 0.3%
 HBSS Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F: Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue, Nat Protoc 2, 2307-11, 2007
Mouse Mouse dentritic Collagenase: 300 u/ml
Deoxyribonuclease I: .002%
 RPMI 1640 Abe Kazumichi, Nguyen KimPhung, Fine SeanD, Mo Ji-Hun, Shen Carol, Shenouda Steve, Corr Maripat, Jung Steffen, Lee Jongdae, Eckmann Lars, Raz Eyal: Conventional dendritic cells regulate the outcome of colonic inflammation independently of T cells., Proc Natl Acad Sci U S A 104, 17022-7, 2007
Mouse Mouse Lympocytes Collagenase/Dispase: 100 u/ml
 RPMI 1640 Annacker O, Coombes JL, Malmstrom V, Uhlig HH, Bourne T, Johansson-Lindbom B, Agace WW, Parker CM, Powrie F.: Essential role for CD103 in the T cell-mediated regulation of experimental colitis, J Exp Med 202, 1051, 2005
Mouse Mouse, 6-8 week Lamina propria lymphocytes Collagenase Type 1: 0.2%
Deoxyribonuclease I: 0.01%
 HBSS Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M: Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis, Am J Physiol/Gastro 284, G595-603, 2003
Mouse Mouse, 6-8 week Lamina propria mononuclear cells Collagenase Type 2: 0.015%
Deoxyribonuclease I: 0.01%
 RPMI Wirtz S., Becker C., Blumberg R., Galle P., and Neurath M.: Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA, J Immunol 168(1), 411, 2002