Colon - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Human human cancer stem cell Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
 RPMI-1640 Phenotypic characterization of human colorectal cancer stem cells. , Proc Natl Acad Sci U S A 104, 10158-63, 2007
Human Human colonic endothelial cells Collagenase Type 2: 0.25%
 HBSS/5%FBS Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells , Am J Physiol/Gastro 282, G1088, 2002
Human human colonocytes Collagenase:
DMEM/F12 Short chain fatty acids differentially modulate cellular phenotype and c-myc protein levels in primary human nonmalignant and malignant colonocytes. , J Nutr 46, 96-105, 2001
Mouse mouse, 6-8 week cancer stem cell Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
 RPMI-1640 Phenotypic characterization of human colorectal cancer stem cells. , Proc Natl Acad Sci U S A 104, 10158-63, 2007
Mouse mouse lympocytes Collagenase/Dispase: 100 u/ml
 RPMI 1640 Essential role for CD103 in the T cell-mediated regulation of experimental colitis. , J Exp Med 202, 1051, 2005
Mouse mouse, 6-8 week lamina propria mononuclear cells Collagenase Type 2: 0.015%
Deoxyribonuclease I: 0.01%
 RPMI Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA. , J Immunol 168(1), 411, 2002