Cartilage - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Steers, 1-2 years Chondrocytes Trypsin: 0.20%
 HBSS Pharmacological Actions of 17 Beta-oestradiol on Articular Cartilage Chondrocytes and Chondrosarcoma Chondrocytes in the Absence of Oestrogen Receptors. , Biochim Biophys Acta 964, 295, 1988
Bovine Calf, 1-14 days Chondrocytes Collagenase Type 2: 0.20%
 PBS Large Scale Preparation of Chondrocytes , Methods Enzymol 58, 560, 1979
Chicken Chick embryos, HH stage Mesenchymal Trypsin: 0.1%
 DMEM
(see reference)		 Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture , In Vitro Cell Dev Biol 29A, 917, 1993
Chicken Chicken, broiler strain, 8-10 weeks Matrix vesicles Epiphyseal growth plate Trypsin: 0.1%
 Tris-buffered saline Differential Fractionation of Matrix Vesicle Proteins: Further Characterization of the Acidic Phospholipid-dependent Ca2+-Binding Proteins , J Biol Chem 265, 4703, 1990
Chicken Chicken, broiler strain, 8-10 week Matrix vesicles Trypsin: 0.1%
 (see reference) Identification of Phospholipid-dependent Calcium-binding Proteins as Constituents of Matrix Vesicles , J Biol Chem 264, 10917, 1989
Chicken Chick embryos, White Leghorn, 19 day old Fibroblasts Epithelial-like Trypsin: 0.25%
 E 199 medium The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro , J Biol Chem 258 (11), 7190, 1983
Chicken Chick embryos, 19 day old Chondrocytes Trypsin: 0.75%
 Coon's modified F-12 The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro , Exp Cell Res 142, 197, 1982
Chicken Chick embryos, White Leghorn Wing buds Trypsin: 0.1%
 Saline G Stage-Related Capacity for Limb Chondrogenesis in Cell Culture , Dev Biol 60, 69, 1977
Human human synoviocytes Collagenase Type 1: 0.4%
 DMEM Soluble Fas ligand inhibits angiogenesis in rheumatoid arthritis. , Arthritis Res Ther 9, R42, 2007
Human human, 64-83 yr chondrocytes Collagenase Type 2: 0.08%
 DMEM/F12 Proliferation and Differentiation Potential of Chondrocytes from Osteoarthritic Patients. , Arthritis Res Ther 7(3), R560, 2005
Human adult synovial Collagenase: 0.15%
Hyaluronidase: 0.1%
Deoxyribonuclease I: 0.015%
 DMEM Diosgenin, a Plant Steroid, Induces Apoptosis in Human Rheumatoid Arthritis Synoviocytes with Cyclooxygenase-2 Overexpression , Arthritis Res Ther 6(4), R373, 2004
Human adult chondrocytes Collagenase Type 2: 0.15%
 DMEM Enzymatic Digestion of Adult Human Articular Cartilage Yields a Small Fraction of the Total Available Cells , Conn Tissue Res 44, 173, 2003
Human human, adults synoviocytes Collagenase Type 1: 0.1%
 RPMI Activation of Nuclear Orphan Receptor NURR1 Transcription by NF-kappa B and Cyclic Adenosine 5'-Monophosphate Response Element-binding Protein in Rheumatoid Arthritis Synovial Tissue , J Immunol 168(6), 2979, 2002
Human Human, 15-60 years chondrocytes Collagenase Type 2: 0.2%
 DMEM Age dependence of cellular properties of human septal cartilage: implications for tissue engineering. , Arch Otolaryngol Head Neck Surg Vol. 127,, , 1248-52, 2001
Human human synovial fibroblasts Collagenase: 0.4%
 DMEM Integrin engagement regulates proliferation and collagenase expression of rheumatoid synovial fibroblasts. , J Immunol 162, 1772-9, 1999
Human human septal chondrocytes Collagenase Type 2: 0.2%
Hyaluronidase: 0.01%
Deoxyribonuclease I: 0.015%
 DMEM/F12 Basic fibroblast growth factor and insulinlike growth factor I support the growth of human septal chondrocytes in a serum-free environment. , Arch Otolaryngol Head Neck Surg Vol. 124,, , 1325-30, 1998
Human Human w/ rheumatoid arthritis Synovial tissue Trypsin: 0.05%
 DMEM
(see reference)		 Production of Collagenase and Prostaglandins by Isolated Adherent Rheumatoid Synovial Cells , Proc Natl Acad Sci U S A 73 (3), 945, 1976
Human Human, 13-62 years Articular chondrocytes Trypsin: 0.2%
 BSS Preliminary Experience with Cell Culture of Human Articular Chondrocytes , Arthritis Rheum 17, 165, 1974
Human Human, 26-84 years Chondrocytes Collagenase:
GBSS Isolation and Culture of Chondrocytes From Human Adult Articular Cartilage , Arthritis Rheum 10, 235, 1967
Mouse mouse, 1 day chondrocytes Collagenase: 0.2%
 DMEM Reduced Chondrocyte Proliferation and Chondrodysplasia in Mice Lacking the Integrin-linked Kinase in Chondrocytes , J Cell Biol 162, 139, 2003
Rabbit rabbit, New Zealand, 1.8-2.3kg chondrocytes Hyaluronidase: .05%
Collagenase Type 2: 0.2%
Trypsin: 0.2%
 Gey's solution Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion. , Ann Rheum Dis 56, 108, 1997
Rabbit Rabbit, New Zealand, white, 4-6 wk & 22-25 wk Chondrocytes Protease XIV: 5 mg/g of tissue
 Ham's F-12 Biosynthesis of Cartilage Proteoglycan & Link Protein Articular Chondrocytes , J Biol Chem 263, 7560, 1988
Rabbit Rabbit, white, male, 8 weeks Chondrocytes Trypsin: 0.2%
 Gey's BSS The Progeny of Rabbit Articular Chondrocytes Synthesize Collagen Types I and III and Type I Trimer, but Not Type II , Biochem 16, 865, 1977
Rabbit Rabbit, New Zealand white or Dutch, 1 week
(also human, newborn)		 Chondrocytes Trypsin: 0.1%
 Saline G, CMF The Synthesis of Cartilage Collagen by Rabbit and Human Chondrocytes in Primary Cell Culture , In Vitro 12, 44, 1976
Rabbit Rabbit, New Zealand white, immature, 2.25 - 3.3 Kg Articular chondrocytes Hyaline Trypsin: 0.2%
 Gey's BSS Articular Cartilage Repair. Behavior of Rabbit Chondrocytes During Tissue Culture and Subsequent Allografting, Clinical Orthopaedics and Related Research,, , 237, 1976
Rabbit Rabbit, New Zealand white, male, 250-350 g Epiphyseal Articular cartilage Trypsin: 0.25%
 Eagle's basal medium Homotransplantation of Isolated Epiphyseal and Articular Cartilage Chondrocytes into Joint Surfaces of Rabbits , Nature 230, 385, 1971
Rat Rat, SD, young, 100-120 g Chondrocytes Trypsin: 0.2%
 Ham's F-12 medium Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib , Calcif Tissue Res 19, 179, 1975
Sheep sheep, 2 month chondrocytes Collagenase Type 2: 0.3%
 Ham's F-12 A composite tissue-engineered trachea using sheep nasal chondrocyte and epithelial cells. , FASEB J 17, 823-8, 2003