Brain - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Microvascular endothelial Dispase: 0.005%
 Medium 199 Glycosphingolipid Antigens in Cultured Microvasular Bovine Brain Endothelial Cells: Sulfoglucuronosyl Paragloboside as a Target of Monoclonal IgM in Demyelinative Neuropathy , J Cell Biol 126 (1), 235, 1994
Bovine Bovine brain endothelial cells Collagenase Type 2: 0.35%
 DMEM Modulation of tight junction structure in blood-brain barrier endothelial cells. Effects of tissue culture, second messengers and cocultured astrocytes. , J Cell Sci 107, 1347, 1994
Bovine Bovine Endothelial Dispase: 0.125%
 MEM Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier, J Tiss Cul Meth 14, 217, 1992
Bovine Bovine, adult Cerebral artery Endothelial Collagenase: 0.2%
 HBSS Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries , In Vitro Cell Dev Biol 26, 291, 1990
Bovine Bovine Capillary endothelial Collagenase: 0.1%
 DMEM Choline Uptake by Cerebral Capillary Endothelial Cells in Culture , J Neurochem 54, 1467, 1990
Bovine Bovine Endothelial Dispase: 0.5%
 MEM Characterization of an In Vitro Blood-Brain Barrier Model System for Studying Drug Transport and Metabolism, Pharm Res 3 (2), 81, 1986
Bovine Calf
(also lamb)		 Oligodendroglia Neural Trypsin: 0.1%
 (see reference) Metabolic Properties of Maintained Oligodendroglia Purified from Brain , J Biol Chem 253, 1592, 1978
Guinea-Pig Guinea-pig, 200-400 g Neurons Trypsin: 0.06-0.08%
 PIPES saline Isolation of Neurons Suitable for Patch-Clamping from Adult Mammalian Central Nervous Systems , J Neurosci Methods 16, 227, 1986
Human human microglia Trypsin: 0.25%
Deoxyribonuclease I: .005%
 DMEM/F12 Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity. , Glia 51, 56-64, 2005
Human Human Microvessels Collagenase Type 4: 0.1%
 DMEM Cutlured Human and Canine Endothelial Cells from Brain Microvessels , Brain Res Bull 21, 785, 1988
Human Human Neuronal Deoxyribonuclease I: 10 µg/ml
 Tris-HCl, 50 mM, CaCl2, 2 mM Isolation and Chemical Characterization of Alzheimer's Disease Paired Helical Filament Cytoskeletons:Differentiation from Amyloid Plaque Core Protein , J Cell Biol 107, 2703, 1988
Human Human, 15-54 years Microvessels Collagenase: 0.1%
 Serum-free modified Lewis medium Isolation and Culture of Cells Derived From Human Cerebral Microvessels , Cell Tissue Res 249, 657, 1987
Monkey monkey, rhesus brain cells Collagenase Type 2: 500 u/ml
Deoxyribonuclease I: 28 u/ml
 HBSS Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection. , J Immunol 167, 5429-38, 2001
Mouse mouse neurosperes PDS kit:
DMEM/F12 Cerebellum- and forebrain-derived stem cells possess intrinsic regional character. , Development 132, 4497, 2005
Mouse mouse, 3 day microglia Papain: 90 u/ml
Deoxyribonuclease I: 2000 u/ml
 Eagle's MEM Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia , J Biol Chem 277, 4816, 2002
Mouse mouse, SD, 8-12 week microglia Trypsin: 0.125%
Collagenase Type 2: 0.01%
Deoxyribonuclease I: .005%
 RPMI-1640 IGF-I and microglia/macrophage proliferation in the ischemic mouse brain , Glia 39, 85, 2002
Mouse mouse cerebellar granule cell precursors Papain: 0.435%
Deoxyribonuclease I: 0.05%
 EBSS A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation. , J Neurosci 20, 5756, 2000
Mouse Mouse Astrocytes Trypsin: 0.25%
 DMEM Interaction Between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis , Proc Natl Acad Sci U S A 96, 7526, 1999
Mouse Mouse, SWR or CF1, 1-3 months Papillae, taste receptor Pronase E: 0.15%
 Carbonate-Phosphate buffer
(see reference)		 A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells , Brain Res 503, 326, 1989
Mouse Mouse, 0-30 day Neural Trypsin NF 1:250: 50 0.25%
 BSS Cultured Cell Systems and Methods for Neurobiology , Methods Enzymol 32, 765, 1974
Mouse mouse, newborn hippocampal cells Papain: 10 u/ml
 DMKM Enhanced hippocampal CA1 LTP but normal spatial learning in inositol 1,4,5-trisphosphate 3-kinase(A)-deficient mice. , Learn Mem Vol. 5,, , 317-30,
Porcine Mini pigs, Yucatan (Susscrofa Yucatan), 4-6 months Microvascular Collagenase: 0.1%
 HBSS Morphologic Plasticity and Periodicity : Porcine Cerebral Microvascular Cells in Culture , In Vitro Cell Dev Biol 26, 169, 1990
Rat rat, E18 hippocampal neurons Papain: 0.2%
 Hibernate Bioenergetic analysis of cerebellar granule neurons undergoing apoptosis by potassium/serum deprivation. , Cell Death Differ Vol. 13,, , 1595-610, 2006
Rat rat, Wistar, 14 day visual cortical PDS kit:
EBSS A rapid increase in the total number of cell surface functional GABAA receptors induced by brain-derived neurotrophic factor in rat visual cortex. , J Biol Chem 278, 44097, 2003
Rat Rat, Wistar, male cerebral endothelial Collagenase Type 3: 0.2%
 MEM Heparan sulfate proteoglycans modulate monocyte migration across cerebral endothelium. , J Neuropathol Exp Neurol 62, 780, 2003
Rat rat, SD, E18 cortical cells Papain: 20 u/ml
Deoxyribonuclease I: .005%
 EBSS GABA(B) receptors mediate motility signals for migrating embryonic cortical cells. , Cerebral Cortex Vol. 11,, , 744-53, 2001
Rat rat, wistar, 250-300 g cerebral artery smooth muscle cells Papain: 1.5 mg/ml
Collagenase Type 4: 1.5 mg/ml
 physiological salt solution Peroxynitrite reversibly inhibits Ca(2+)-activated K(+) channels in rat cerebral artery smooth muscle cells. , Am J Physiol Heart Circ Physiol 278, H1883, 2000
Rat rat, SD, E16 cortical neurons PDS kit: with modifications
 neurobasal medium SIB-1757 and SIB-1893: selective, noncompetitive antagonists of metabotropic glutamate receptor type 5. , J Pharmacol Exp Ther 290, 170, 1999
Rat rat, wistar, neonatal hippocampal cells Papain: 1 mg/ml
 DMEM Somatostatin inhibits excitatory transmission at rat hippocampal synapses via presynaptic receptors , J Neurosci 17, 4066, 1997
Rat SD, Fisher hippocampal neurons Papain: 0.2%
 HibernateA/B27 Isolation and culture of adult rat hippocampal neurons of any age. , J Neurosci Methods 71, 143, 1997
Rat Rat, E18 hippocampal and cortical neurons PDS kit:
Neurobasal media Neuritin: a gene induced by neural activity and neurotrophins that promotes neuritogenesis. , Proc Natl Acad Sci U S A 94, 2648-53, 1997
Rat Rat, SD, newborn Glial Trypsin: 0.0625%
 MEM, sterile The Olfactory Nerve Contains Two Populations of Glia , In Vitro 5, 269, 1992
Rat Rat, newborn Astrocytes Trypsin: 0.25%
 DMEM, HBSS Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes , Glia 5, 239, 1992
Rat Rat, 60-72 hours old Fibroblasts Trypsin: 0.2%
 HEPES buffered DMEM Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF , Neuron 7, 265, 1991
Rat Rat, SD, adult Endothelial Collagenase Type 2: 0.5%
 Medium 199 Extracellular Matrix Permits the Expression of Von Willebrand's Factor, Uptake of Di-I-Acetylated Low Density Lipoprotein and Secretion of Prostacyclin in Cultures of Endothelial Cells from Rat Brain Microvessels , In Vitro Cell Dev Biol 27A, 689, 1991
Rat Rat, SD, female Fetal rat brain Collagenase Type 4: 0.1%
 DMEM Serum Proteins Enhance Aggregate Formation of Dissociated Fetal Rat Brain Cells in an Aggregating Culture , In Vitro Cell Dev Biol 24 (10), 1031, 1988
Rat Rat, albino, adult and newborn Cerebral cortices Trypsin: 0.25%
 PBS Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain , J Neurosci 6, 2163, 1986
Rat Rat, Wistar, newborn Germinal matrix Trypsin: 0.25%
 HBSS Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture , J Neurosci 6, 52, 1986
Rat Rat, fetus, 18-20 day Hippocampal neurons Trypsin: 0.25%
 HBSS, CMF An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts , J Neurosci 4, 1944, 1984
Rat Rat, fetus, 18 day Hippocampi Trypsin: 0.1%
 HBSS Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death , J Neurosci 4 (7), 1884, 1984
Rat Rat, SD, 19-20 days pregnant Neural Trypsin: 0.25%
 DMEM Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture , J Neurosci 3 (12), 2448, 1983
Rat Rat, SD, fetal Cerebral cortex Hypothalmus Deoxyribonuclease I: 0.001%
 HEPES High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell , Brain Res 239, 463, 1982
Rat Rat, Wistar-Kyoto, male, 100 - 200 g Endothelial Cerebral Collagenase Type 2: 0.05%
 HBSS Primary Culture of Rat Cerebral Microvascular Endothelial Cells. , Lab Invest 46, 554, 1982
Rat Rat, fetus Cortical Trypsin: 0.25%
 Puck's D1 (see reference) A Method for Large-scale Production of Mouse Brain Cortical Cultures , Brain Res 255, 361, 1982
Rat Rat, Wistar, pregnant Glial Trypsin: 0.05%
 Eagle's MEM/DMEM Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain , Dev Biol 83, 301, 1981
Rat Rat, SD, one month old Capillary endothelium Pericytes Dispase: 0.1%
 Medium 199 Primary Culture of Capillary Endothelium From Rat Brain , In Vitro 17 (4), 353, 1981
Rat Rat, Wistar, male, 300-500 g Microvessels Endothelial Collagenase Type 2: 0.75%
 Ringers-HEPES buffer Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity , J Neurochem 35 (2), 374, 1980
Rat Rat, Wistar, adult, 170 g Endothelial Trypsin: 0.5%
 BSS Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter , J Anat 129, 261, 1979
Rat Rat, Holtzmann, 18 day Hippocampal neurons Trypsin: 0.1%
 HBSS Rat Hippocampal Neurons in Dispersed Cell Culture , Brain Res 126, 397, 1977
Rat Rat, SD, 2 days Pineal Trypsin: 0.25%
 DMEM, MEM Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons. , Exp Cell Res 104, 345, 1977
Sheep Lamb
(also calf)		 Oligodendroglia Neural Trypsin: 0.1%
 (see reference) Metabolic Properties of Maintained Oligodendroglia Purified from Brain , J Biol Chem 253, 1592, 1978
Shellfish Helisoma trivolvis Neurons Buccal ganglia Trypsin: 0.2%
 Saline, sterile Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation , J Neurosci 5, 3145, 1985