Bone - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Chondrocytes Collagenase Type 2: 0.4%
 DMEM Buschmann, M., Gluzband, Y., Grodzinsky, A., and Hunziker, E.: Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture, J Cell Sci 108, 1497, 1995
Chicken Chick, day old Osteoblasts Trypsin: 0.03%
 DMEM Gay, C., Lloyd, Q., and Gilman, V.: Characteristics and Culture of Osteoblasts Derived From Avian Long Bone, In Vitro Cell Dev Biol 30A, 379, 1994
Chicken Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus) Chondrocytes Trypsin: 0.25%
 Ham's F12 Rosselot, G., Reginato, A.M., and Leach, R.M.: Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes, In Vitro Cell Dev Biol 28A, 235, 1992
Chicken Chick, embryo Vertebrae chondroblasts Trypsin: 0.25%
 Simm's, CMF Schiltz, J.R., Mayne R., and Holtzer, H.: The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture, Differentiation 1, 97, 1973
Human Human, 22-73 year Osteoblasts Trypsin: 0.5%
 Basal Medium Kneser U, Voogd A, Ohnolz J, Buettner O, Stangenberg L, Zhang YH, Stark GB, Schaefer DJ: Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute, Cells Tissues Organs 179, 158-69, 2005
Human Human, 60+ year Bone cells, osteoblasts Collagenase Type 2: 200-250 u/ml
 DMEM Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage, J Bone Miner Res 14, 362, 1999
Human Human Osteoblasts Trypsin: 0.1%
 DMEM Meikle, M., Boyd, S., Hembry, R., Compston, J., Croucher, P., and Reynolds, J.: Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines, J Cell Sci 103, 1093, 1992
Human Human Osteoblasts Collagenase Type 4: 250 u/ml
 DMEM Fedarko, N.S., Termine, J.D., Young, M.F. and Robey, P.G.: Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro, J Biol Chem 265, 12200, 1990
Mouse Mouse, male, 6-8 week old Osteoclasts Collagenase Type 3: 0.1%
 DMEM Sakai, E., Miyamoto, H., Okamoto, K., Kato, Y., Yamamoto, K., and Sakai, H.: Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages, J Biochem (Tokyo) 130, 823, 2001
Mouse Mouse, 3-5 day, 6-8 week Bone cells, osteoblasts Collagenase Type 2: 200-250 u/ml
 DMEM Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage, J Bone Miner Res 14, 362, 1999
Mouse Mouse, BALB/c Osteoblastlike cells
Stromal cell lines
Hematopoietic blast cells		 Trypsin: 0.1%
 Eagle's MEM Takanashi, H., Matsuishi, T., and Yoshizato, K.: Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells, In Vitro Cell Dev Biol 30A, 384, 1994
Mouse Mouse, Swiss-Webster Neonatal bone Collagenase Type 2: 0.20%
 Tris-buffered saline Chen,T. and Feldman,D.: Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells, J Biol Chem 256, 5561, 1981
Rat Rat, fetal, 21 days of gestation Calvaria Collagenase: 0.2%
 MEM Owen, T., Aronow, M., Shalhoub, V., Barone, L., Wilming, L., Tassinari, M., Kennedy, M., Pockwinse, S., Lian, J., and Stein, G.: Progressive Development of the Rat Oseoblast Phenotype In Vitro: Reciprocal Relationships in Expression of Genes Associated with Osteoblast Proliferation and Differentiation During Formation of the Bone Extracellular Matrix, J Cell Physiol 143, 420, 1990
Rat Rat, newborn Osteoblastlike cells Collagenase Type 2: 0.3%
 MEM Ernst, M., and Froesch, E.: Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I, Calcif Tissue Int 40, 27, 1987
Rat Rat, fetus, 17-21 day calvaria Collagenase: 0.01%-0.6%
 Tris-buffered saline Peck, W., Birge, S., and Fedak, S.: Bone Cells: Biochemical and Biological Studies After Enzymatic Isolation, Science 146, 1476, 1964