Bone - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Chondrocytes Collagenase Type 2: 0.4%
 DMEM Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture , J Cell Sci 108, 1497, 1995
Chicken Chick, day old Osteoblasts Trypsin: 0.03%
 DMEM Characteristics and Culture of Osteoblasts Derived From Avian Long Bone , In Vitro Cell Dev Biol 30A, 379, 1994
Chicken Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus) Chondrocytes Trypsin: 0.25%
 Ham's F12 Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes , In Vitro Cell Dev Biol 28A, 235, 1992
Chicken Chick, embryo Vertebrae chondroblasts Trypsin: 0.25%
 Simm's, CMF The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture, Differentiation 1, 97, 1973
Human 60+ year bone cells, osteoblasts Collagenase Type 2: 200-250 u/ml
 DMEM Thy-1 antigen Expression by Cells in the Osteoblast Lineage , J Bone Miner Res 14, 362, 1999
Human Human Osteoblasts Trypsin: 0.1%
 DMEM Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines , J Cell Sci 103, 1093, 1992
Human Human Osteoblasts Collagenase Type 4: 250 u/ml
 DMEM Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro , J Biol Chem 265, 12200, 1990
Mouse Mouse, male, 6-8 week old Osteoclasts Collagenase Type 3: 0.1%
 DMEM Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages , J Biochem (Tokyo) 130, 823, 2001
Mouse 3-5 day, 6-8 week bone cells, osteoblasts Collagenase Type 2: 200-250 u/ml
 DMEM Thy-1 antigen Expression by Cells in the Osteoblast Lineage , J Bone Miner Res 14, 362, 1999
Mouse Mouse, BALB/c Osteoblastlike cells Stromal cell lines Hematopoietic blast cells Trypsin: 0.1%
 Eagle's MEM Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells , In Vitro Cell Dev Biol 30A, 384, 1994
Mouse Mouse, Swiss-Webster Neonatal bone Collagenase Type 2: 0.20%
 Tris-buffered saline Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells , J Biol Chem 256, 5561, 1981
Rat Rat, fetal, 21 days of gestation Calvaria Collagenase: 0.2%
 MEM Progressive Development of the Rat Oseoblast Phenotype In Vitro: Reciprocal Relationships in Expression of Genes Associated with Osteoblast Proliferation and Differentiation During Formation of the Bone Extracellular Matrix , J Cell Physiol 143, 420, 1990
Rat Rat, newborn Osteoblastlike cells Collagenase Type 2: 0.3%
 MEM Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I , Calcif Tissue Int 40, 27, 1987