Bone - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Chondrocytes Collagenase Type 2: 0.4%
 DMEM Buschmann, M., Gluzband, Y., Grodzinsky, A., and Hunziker, E.: Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture, J. Cell Sci. 108, 1497, 1995
Chicken Chick, day old Osteoblasts Trypsin: 0.03%
 DMEM Gay, C., Lloyd, Q., and Gilman, V.: Characteristics and Culture of Osteoblasts Derived From Avian Long Bone, In Vitro Cell. Dev. Biol. 30A, 379, 1994
Chicken Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus) Chondrocytes Trypsin: 0.25%
 Ham's F12 Rosselot, G., Reginato, A.M., and Leach, R.M.: Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes, In Vitro Cell. Dev. Biol. 28A, 235, 1992
Chicken Chick, embryo Vertebrae chondroblasts Trypsin: 0.25%
 Simm's, CMF Schiltz, J.R., Mayne R., and Holtzer, H.: The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture, Differentiation 1, 97, 1973
Human Human, 22-73 year Osteoblasts Trypsin: 0.5%
 Basal Medium Kneser U, Voogd A, Ohnolz J, Buettner O, Stangenberg L, Zhang YH, Stark GB, Schaefer DJ: Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute, Cells Tissues Organs 179, 158-69, 2005
Human Human, 60+ year Bone cells, osteoblasts Collagenase Type 2: 200-250 u/ml
 DMEM Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage, J. Bone Miner. Res. 14, 362, 1999
Human Human Osteoblasts Trypsin: 0.1%
 DMEM Meikle, M., Boyd, S., Hembry, R., Compston, J., Croucher, P., and Reynolds, J.: Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines, J. Cell Sci. 103, 1093, 1992
Human Human Osteoblasts Collagenase Type 4: 250 u/ml
 DMEM Fedarko, N.S., Termine, J.D., Young, M.F. and Robey, P.G.: Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro, J. Biol. Chem. 265, 12200, 1990
Mouse Mouse, male, 6-8 week old Osteoclasts Collagenase Type 3: 0.1%
 DMEM Sakai, E., Miyamoto, H., Okamoto, K., Kato, Y., Yamamoto, K., and Sakai, H.: Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages, J. Biochem. (Tokyo) 130, 823, 2001
Mouse Mouse, 3-5 day, 6-8 week Bone cells, osteoblasts Collagenase Type 2: 200-250 u/ml
 DMEM Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage, J. Bone Miner. Res. 14, 362, 1999
Mouse Mouse, BALB/c Osteoblastlike cells
Stromal cell lines
Hematopoietic blast cells		 Trypsin: 0.1%
 Eagle's MEM Takanashi, H., Matsuishi, T., and Yoshizato, K.: Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells, In Vitro Cell. Dev. Biol. 30A, 384, 1994
Mouse Mouse, Swiss-Webster Neonatal bone Collagenase Type 2: 0.20%
 Tris-buffered saline Chen,T. and Feldman,D.: Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells, J. Biol. Chem. 256, 5561, 1981
Rat Rat, fetal, 21 days of gestation Calvaria Collagenase: 0.2%
 MEM Owen, T., Aronow, M., Shalhoub, V., Barone, L., Wilming, L., Tassinari, M., Kennedy, M., Pockwinse, S., Lian, J., and Stein, G.: Progressive Development of the Rat Oseoblast Phenotype In Vitro: Reciprocal Relationships in Expression of Genes Associated with Osteoblast Proliferation and Differentiation During Formation of the Bone Extracellular Matrix, J. Cell. Physiol. 143, 420, 1990
Rat Rat, newborn Osteoblastlike cells Collagenase Type 2: 0.3%
 MEM Ernst, M., and Froesch, E.: Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I, Calcif. Tissue Int. 40, 27, 1987
Rat Rat, fetus, 17-21 day calvaria Collagenase: 0.01%-0.6%
 Tris-buffered saline Peck, W., Birge, S., and Fedak, S.: Bone Cells: Biochemical and Biological Studies After Enzymatic Isolation, Science 146, 1476, 1964