Bone - Tissue Tables
| Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
| Bovine
|
Bovine |
Chondrocytes |
Collagenase Type 2: 0.4%
|
DMEM |
Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture
,
J Cell Sci
108,
1497, 1995 |
| Chicken
|
Chick, day old |
Osteoblasts |
Trypsin: 0.03%
|
DMEM |
Characteristics and Culture of Osteoblasts Derived From Avian Long Bone
,
In Vitro Cell Dev Biol
30A,
379, 1994 |
| Chicken
|
Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus) |
Chondrocytes |
Trypsin: 0.25%
|
Ham's F12 |
Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes
,
In Vitro Cell Dev Biol
28A,
235, 1992 |
| Chicken
|
Chick, embryo |
Vertebrae chondroblasts |
Trypsin: 0.25%
|
Simm's, CMF |
The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture,
Differentiation
1,
97, 1973 |
| Human
|
60+ year |
bone cells, osteoblasts |
Collagenase Type 2: 200-250 u/ml
|
DMEM |
Thy-1 antigen Expression by Cells in the Osteoblast Lineage
,
J Bone Miner Res
14,
362, 1999 |
| Human
|
Human |
Osteoblasts |
Trypsin: 0.1%
|
DMEM |
Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines
,
J Cell Sci
103,
1093, 1992 |
| Human
|
Human |
Osteoblasts |
Collagenase Type 4: 250 u/ml
|
DMEM |
Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro
,
J Biol Chem
265,
12200, 1990 |
| Mouse
|
Mouse, male, 6-8 week old |
Osteoclasts |
Collagenase Type 3: 0.1%
|
DMEM |
Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages
,
J Biochem (Tokyo)
130,
823, 2001 |
| Mouse
|
3-5 day, 6-8 week |
bone cells, osteoblasts |
Collagenase Type 2: 200-250 u/ml
|
DMEM |
Thy-1 antigen Expression by Cells in the Osteoblast Lineage
,
J Bone Miner Res
14,
362, 1999 |
| Mouse
|
Mouse, BALB/c |
Osteoblastlike cells
Stromal cell lines
Hematopoietic blast cells |
Trypsin: 0.1%
|
Eagle's MEM |
Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells
,
In Vitro Cell Dev Biol
30A,
384, 1994 |
| Mouse
|
Mouse, Swiss-Webster |
Neonatal bone |
Collagenase Type 2: 0.20%
|
Tris-buffered saline |
Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells
,
J Biol Chem
256,
5561, 1981 |
| Rat
|
Rat, fetal, 21 days of gestation |
Calvaria |
Collagenase: 0.2%
|
MEM |
Progressive Development of the Rat Oseoblast Phenotype In Vitro: Reciprocal Relationships in Expression of Genes Associated with Osteoblast Proliferation and Differentiation During Formation of the Bone Extracellular Matrix
,
J Cell Physiol
143,
420, 1990 |
| Rat
|
Rat, newborn |
Osteoblastlike cells |
Collagenase Type 2: 0.3%
|
MEM |
Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I
,
Calcif Tissue Int
40,
27, 1987 |
|
|