Adrenal - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Chromaffin Collagenase: 0.2%
 Locke's solution Short-term Immunosuppression Enhances Long-term Survival of Bovine Chromaffin Cell Xenografts in Rat CNS , Cell Transplant 1, 33, 1992
Bovine Bovine Chromaffin Deoxyribonuclease I: 30 u/mg
 HEPES Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process , J Pharmacol Exp Ther 263 (3), 1479, 1992
Bovine Bovine
(also rat)		 Heart Adrenal chromaffin Paraneurons Trypsin: 0.06%
 25mM HEPES buffered Locke's solution, CMF Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons , In Vitro Cell Dev Biol 26, 335, 1990
Bovine Bovine Chromaffin Collagenase Type 1: 0.25 %
 DMEM Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells , J Neurochem 54 (3), 931, 1990
Bovine Bovine Chromaffin Collagenase: 0.05%
 Locke's solution, CMF Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture, Neurochem Int 7, 89, 1985
Bovine Bovine Chromaffin Collagenase: 0.15%
 Kreb's, CMF Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells , Br J Biomed Sci 81, 599, 1984
Bovine Bovine Chromaffin Collagenase: 0.1%
 (see reference) An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells , J Biol Chem 259, 1114, 1984
Bovine Bovine Chromaffin Collagenase Type 1: 0.05%
 CF Kreb's Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells , Br J Pharmacol 81, 119, 1984
Bovine Bovine Chromaffin Collagenase Type 1: 0.025%
 HBSS, modified Bovine Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture , J Neurosci Methods 7, 329, 1983
Bovine Bovine Medulla Hyaluronidase: 0.2%
 Saline w/BSA 0.5% Stimulus-Secretion Coupling in Isolated Bovine Adrenal Medullary Cells , Q J Exp Physiol 68, 123, 1983
Bovine Bovine Medulla Collagenase: 0.2%
 Krebs-Ringer bicarbonate buffer, CMF alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells , Cell Tissue Res 226, 655, 1982
Bovine Bovine Chromaffin Deoxyribonuclease I: 15 µg/ml
 Medium A
(see reference)		 Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium , Exp Cell Res 133, 159, 1981
Bovine Bovine Medulla Protease: 0.2%
 Saline Calcium Control of Exocytosis and Endocytosis in Bovine Adrenal Medullary Cells , Phil Trans R Soc Lond 296, 83, 1981
Bovine Bovine Chromaffin Collagenase: 0.05%
 Locke's solution, CMF Morphological Characteristics and Stimulus-Secretion Coupling in Bovine Adrenal Chromaffin Cell Cultures , Neuroscience 5, 1533, 1980
Bovine Bovine, adult
(also rat, Hanover-Wistar, young; guinea-pig, newborn)		 Chromaffin Collagenase: 0.5%
 HBSS Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro , Adv Biochem Psychopharmacol 255, 51, 1980
Bovine Bovine Chromaffin Collagenase: 0.25%
 F-12 medium Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells , Nature 283, 489, 1980
Bovine Bovine Medulla Collagenase: 0.05%
 Locke's solution, CF Stability of Bovine Adrenal Medulla Cells in Culture , J Neurochem 35 (3), 679, 1980
Bovine Bovine, adult Medulla Collagenase Type 1: 0.5%
 HBSS Establishment, Characterization and Fibre Outgrowth of Isolated Bovine Adrenal Medullary Cells in Long-Term Cultures , Neuroscience 5, 1445, 1980
Bovine Bovine Chromaffin Collagenase: 0.05%
 DMEM Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells , Neuroscience 5, 2261, 1980
Bovine Bovine Medullary Collagenase Type 2: 0.2%
 HEPES Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured Bovine Adrenal Medullary Cells , J Pharmacol Exp Ther 209 (1), 147, 1979
Bovine Bovine Foreskin Collagenase: 0.5%
 Dulbecco's modified Eagle's medium w/10% calf serum Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
Bovine Bovine Medulla Collagenase Type 1: 0.05%
 Kreb's, CF Functional and Morphological Characterization of Isolated Bovine Adrenal Medullary Cell , J Cell Biol 76, 12, 1978
Bovine Bovine Chromaffin Hyaluronidase: 0.2%
 HEPES, CF The Isolated Bovine Adrenomedullary Chromaffin Cell: A Model of Neuronal Excitation-Secretion , Endocrinology 101, 1369, 1977
Guinea-Pig Guinea-pig, 500-700 g Chromaffin Medulla Collagenase:
BSS
(see reference)		 Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of Guinea-pig Adrenal Medulla , Neurochem Int 6, 1813, 1981
Guinea-Pig Guinea-pig
(also rat, Hanover-Wistar, young; newborn; cattle)		 Chromaffin Collagenase: 0.5%
 HBSS Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro , Adv Biochem Psychopharmacol 255, 51, 1980
Guinea-Pig Guinea-pig Adrenal Chromaffin Collagenase: 0.05%-0.20%
 Kreb's-Ringer bicarb glucose buffer, CF Catecholamine Secretion by Isolated Adrenal Cells , Biochim Biophys Acta 421, 168, 1976
Hamster Hamster (Mesocricetus auratus) 100-150 g Adrenal Chromaffin Hyaluronidase: 0.20%
 Kreb's Ringer bicarbonate buffer Catecholamine Secretion by Hamster Adrenal Cells , J Neurochem 32, 927, 1979
Human Human, adult Chromaffin Trypsin: 0.25%
 Eagle's MEM Nerve Growth Factor-Induced Neurite Outgrowth from Normal Human Chromaffin Cells , Lab Invest 43, 399, 1980
Human Human, adult and child Foreskin Collagenase: 0.5%
 Dulbecco's modified Eagle's medium w/10% calf serum Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
Mouse mouse, embryonic chromafin cells Papain: 20-25 u/ml
 DMEM The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells. , J Neurosci 25, 10546-55, 2005
Rat rat, SD, male zona fasciculata/reticularis Collagenase: 0.4%
 Krebs-HEPES An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells. , Am J Physiol Regul Integr Comp Physiol Vol. 284,, , R1631-5, 2003
Rat Rat, SD, male ZG ZFR Collagenase Type 1: 0.2%
 Kreb's Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis , J Endocrinol 154, 449, 1997
Rat Rat, Fischer, male, 10-16 weeks Adrenocortical Deoxyribonuclease I: 0.005%
 BSS Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture , In Vitro Cell Dev Biol 26, 493, 1990
Rat Rat, male, 120-160 g,
Rat, SD, male, 400-450 g		 Leydig Adrenal Collagenase Type 2: 0.03% (adrenal)
 Krebs Ringer bicarbonate buffer Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis , In Vitro Cell Dev Biol 26, 24, 1990
Rat Rat, SD, 2-4 day old
(also bovine)		 Heart Adrenal chromaffin Paraneurons Trypsin: 0.06%
 25mM HEPES buffered Locke's solution, CMF Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons , In Vitro Cell Dev Biol 26, 335, 1990
Rat Rat, Long-Evans, female, 150-200 g Glomerulosa Collagenase: 0.2%
 MEM-d-Val Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture , J Steroid Biochem 20, 449, 1984
Rat Rat, Fischer, male, 1-10 months Adrenocortical Deoxyribonuclease I: 0.005%
 BSS Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells , In Vitro 19, 46, 1983
Rat Rat, SD, male, 400-450 g Decapular Capsular Glomerulosa Deoxyribonuclease I: 0.01%
 Medium 199 Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells , Int J Pept Protein Res 19, 361, 1982
Rat Rat Chromaffin Trypsin: 0.10%
 Ham's F-12 w/HEPES An Optical Study of Isolated Rat Adrenal Chromaffin Cells , Exp Cell Res 125, 369, 1980
Rat Rat, Hanover-Wistar, 2nd postnatal week (also guinea-pig, cattle) Chromaffin Collagenase: 0.5%
 HBSS Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells , Adv Biochem Psychopharmacol 25, 51, 1980
Rat Rat , SD, female, 200 g Glomerulosa Deoxyribonuclease I: 0.05%
 Kreb's The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells , Endocrinology 106 (1), 50, 1980
Rat Rat Foreskin Collagenase: 0.5%
 Dulbecco's modified Eagle's medium w/10% calf serum Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
Rat Rat, Wistar-Hanover, 7-12 day Medullary Trypsin: 0.125%
 HBSS Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids
 , Proc Natl Acad Sci U S A 75 (7), 3498, 1978
Rat Rat, SD, male Cortical Trypsin: 0.25%
 Kreb's Ringer bicarbonate buffer Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands , Exp Cell Res 79, 263, 1973
Rat Rat, Holtzman, male, 180-250 g Adrenal Collagenase Type 1: 0.5%
 Kreb's Ringer bicarbonate buffer A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism , Endocrinology 82, 1053, 1968