Adrenal - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Chromaffin Collagenase Type 1: 0.1-0.2%
Deoxyribonuclease I: 0.003-0.015%
Hyaluronidase: 0.015%
 DMEM/F12 Dominguez, N., Rodri­guez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012
Bovine Bovine, 6 month Chromaffin Collagenase Type 1: 0.125%
 Locke's solution Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B: Viability and functionality of bovine chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core, Cell Transplant 15, 121-33, 2006
Bovine Bovine Chromaffin cells Collagenase: 0.1%
Deoxyribonuclease I: 30 u/ml
 DMEM Hahm, S., Chen, Y., Vinson, C. and Eiden, L.: A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization., Mol Pharmacol 64, 1503, 2003
Bovine Bovine Chromaffin Collagenase: 0.2%
 Locke's solution Ortega, J., Sagen, J., and Pappas, G.: Short-term Immunosuppression Enhances Long-term Survival of Bovine Chromaffin Cell Xenografts in Rat CNS, Cell Transplant 1, 33, 1992
Bovine Bovine Chromaffin Deoxyribonuclease I: 30 u/mg
 HEPES Zhu, J., Li, W., Toews, M., and Hexum, T.: Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process, J Pharmacol Exp Ther 263 (3), 1479, 1992
Bovine Bovine
(also rat)		 Heart
Adrenal chromaffin
Paraneurons		 Trypsin: 0.06%
 25mM HEPES buffered Locke's solution, CMF Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
Bovine Bovine Chromaffin Collagenase Type 1: 0.25 %
 DMEM Dahmer, M., Hart, P., and Perlman, R.: Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells, J Neurochem 54 (3), 931, 1990
Bovine Bovine Chromaffin Collagenase: 0.2%
Deoxyribonuclease I: 0.015%
 Locke's solution Higgins, L., and Berg, D.: Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells, J Neurosci 7 (6), 1792, 1987
Bovine Bovine Chromaffin Collagenase: 0.05%
 Locke's solution, CMF Aunis, D., Rotllan, P., and Miras-Portugal, M.: Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture, Neurochem Int 7, 89, 1985
Bovine Bovine Chromaffin Collagenase: 0.15%
 Kreb's, CMF Almazan, G., Aunis, D., Garcia, A., Montiel, C., Nicolas, G., and Sanchez-Garcia, P.: Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells, Br J Biomed Sci 81, 599, 1984
Bovine Bovine Chromaffin Collagenase: 0.1%
 (see reference) Pollard, H., Pazoles, C., Creutz, C., Scott, J., Zinder, O., and Hotchkiss, A.: An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells, J Biol Chem 259, 1114, 1984
Bovine Bovine Chromaffin Collagenase Type 1: 0.05%
 CF Kreb's Cena, V., Garcia, A., Montiel, C., and Sanchez-Garcia, P.: Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells, Br J Pharmacol 81, 119, 1984
Bovine Bovine Chromaffin Collagenase Type 1: 0.025%
 HBSS, modified Waymire, J., Bennett, W., Boehme, R., Hankins, L., Gilmer-Waymire, K., and Haycock, J.: Bovine Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture, J Neurosci Methods 7, 329, 1983
Bovine Bovine Medulla Hyaluronidase: 0.2%
 Saline w/BSA 0.5% Knight, D. and Baker, P.: Stimulus-Secretion Coupling in Isolated Bovine Adrenal Medullary Cells, Q J Exp Physiol 68, 123, 1983
Bovine Bovine Medulla Collagenase: 0.2%
 Krebs-Ringer bicarbonate buffer, CMF Greenberg, A. and Zinder, O.: alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells, Cell Tissue Res 226, 655, 1982
Bovine Bovine Chromaffin Deoxyribonuclease I: 15 µg/ml
 Medium A
(see reference)		 Wilson, S.P., and Viveros, O.H.: Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium, Exp Cell Res 133, 159, 1981
Bovine Bovine Medulla Protease: 0.2%
 Saline Baker, P., and Knight., D: Calcium Control of Exocytosis and Endocytosis in Bovine Adrenal Medullary Cells, Phil Trans R Soc Lond 296, 83, 1981
Bovine Bovine Chromaffin Collagenase: 0.05%
 Locke's solution, CMF Trifaro, J.M., and Lee, R.W.: Morphological Characteristics and Stimulus-Secretion Coupling in Bovine Adrenal Chromaffin Cell Cultures, Neuroscience 5, 1533, 1980
Bovine Bovine, adult
(also rat, Hanover-Wistar, young; guinea-pig, newborn)		 Chromaffin Collagenase: 0.5%
Collagenase: 0.5%
 HBSS Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255, 51, 1980
Bovine Bovine Chromaffin Collagenase: 0.25%
 F-12 medium Kumakura, K., Karoum, F., Guidotti, A., and Costa, E.: Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells, Nature 283, 489, 1980
Bovine Bovine Medulla Collagenase: 0.05%
 Locke's solution, CF Kilpatrick, D., Ledbetter, F., Carson, K., Kirshner, A., Slepetis, R., and Kirshner, N.: Stability of Bovine Adrenal Medulla Cells in Culture, J Neurochem 35 (3), 679, 1980
Bovine Bovine, adult Medulla Collagenase Type 1: 0.5%
 HBSS Unsicker, K., and Griesser, G.: Establishment, Characterization and Fibre Outgrowth of Isolated Bovine Adrenal Medullary Cells in Long-Term Cultures, Neuroscience 5, 1445, 1980
Bovine Bovine Chromaffin Collagenase: 0.05%
 DMEM Aunis, D., Guerold, B., Bader, M-F., and Cieselski-Treska, J.: Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells, Neuroscience 5, 2261, 1980
Bovine Bovine Medullary Collagenase Type 2: 0.2%
 HEPES Hersey, R., and DiStefano, V.: Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured Bovine Adrenal Medullary Cells, J Pharmacol Exp Ther 209 (1), 147, 1979
Bovine Bovine Foreskin Collagenase: 0.5%
 Dulbecco's MEM w/10% calf serum Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979
Bovine Bovine Medulla Collagenase Type 1: 0.05%
 Kreb's, CF Fenwick, E., Fajdiga, P., Howe, N., and Livett, B.: Functional and Morphological Characterization of Isolated Bovine Adrenal Medullary Cell, J Cell Biol 76, 12, 1978
Bovine Bovine Chromaffin Hyaluronidase: 0.2%
 HEPES, CF Brooks, J.C.: The Isolated Bovine Adrenomedullary Chromaffin Cell: A Model of Neuronal Excitation-Secretion, Endocrinology 101, 1369, 1977
Bovine Bovine, male Chromaffin Collagenase Type 1: 0.2%
 Kreb's Ringer bicarbonate buffer, CF Schneider, A., Herz, R., and Rosenheck, K.: Stimulus-Secretion Coupling in Chromaffin Isolated From Adrenal Medulla, Proc Natl Acad Sci U S A 74, 5036, 1977
Guinea-Pig Guinea-pig, 500-700 g Chromaffin
Medulla		 Collagenase:
BSS
(see reference)		 Role, L.W., Leeman, S.E., and Perlman, R.L.: Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of Guinea-pig Adrenal Medulla, Neurochem Int 6, 1813, 1981
Guinea-Pig Guinea-pig
(also rat, Hanover-Wistar, young; newborn; cattle)		 Chromaffin Collagenase: 0.5%
Collagenase: 0.5%
 HBSS Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255, 51, 1980
Guinea-Pig Guinea-pig Adrenal
Chromaffin		 Collagenase: 0.05%-0.20%
 Kreb's-Ringer bicarb glucose buffer, CF Hochman, J., and Perlman, R.L.: Catecholamine Secretion by Isolated Adrenal Cells, Biochim Biophys Acta 421, 168, 1976
Hamster Hamster (Mesocricetus auratus) 100-150 g Adrenal
Chromaffin		 Hyaluronidase: 0.20%
 Kreb's Ringer bicarbonate buffer Liang, B.T., and Perlman, R.L.: Catecholamine Secretion by Hamster Adrenal Cells, J Neurochem 32, 927, 1979
Human Human Adrenal medulla progenitor Collagenase: 0.2%
 DMEM/F-12 Santana, M., Chung, K., Vukicevic, V., Rosmaninho-Salgado, J., Kanczkowski, W., Cortez, V., Hackmann, K., Bastos, C., Mota, A., Schrock, E., Bornstein, S., Cavadas, C. and Ehrhart-Bornstein, M.: Isolation, Characterization, and Differentiation of Progenitor Cells from Human Adult Adrenal Medulla., Stem Cells Transl Med 1, 783, 2012
Human Human Chromaffin cells Collagenase: 0.2%
 Locke's solution Jeon, Y., Baek, W., Chung, S., Shin, N., Kim, H., and Lee, S.: Cultured Human Chromaffin Cells Grafted in Spinal Subarachnoid Space Relieves Allodynia in a Pain Rat Model., Korean J Anesthesiol 60, 357, 2011
Human Human Adrenocortical Collagenase Type 1: 0.2%
Deoxyribonuclease I: 0.01%
 Krebs Ringer Caroccia, B., Fassina, A., Seccia, T., Recarti, C., Petrelli, L., Belloni, A., Pelizzo, M. and Rossi, G.: Isolation of Human Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method., Endocrinology 151, 1375, 2010
Human Human, adult Chromaffin Trypsin: 0.25%
 Eagle's MEM Tischler, A., DeLellis, R., Bailes, B., Nunnemacher, G.,Carabba, V., and Wolfe, H.: Nerve Growth Factor-Induced Neurite Outgrowth from Normal Human Chromaffin Cells, Lab Invest 43, 399, 1980
Human Human, adult and child Foreskin Collagenase: 0.5%
 Dulbecco's MEM w/10% calf serum Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979
Mouse Mouse Chromaffin Collagenase Type 1: 0.1-0.2%
Deoxyribonuclease I: 0.003-0.015%
Hyaluronidase: 0.015%
 DMEM/F12 Dominguez, N., Rodri­guez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012
Mouse Mouse, 8-10 week Chromaffin Papain: 40 u/ml
 DMEM Kolski-Andreaco, A., Cai, H., Currle, D., Chandy, K. and Chow, R.: Mouse Adrenal Chromaffin Cell Isolation., J Vis Exp 2, 129, 2007
Mouse Mouse, embryonic Chromafin cells Papain: 20-25 u/ml
 DMEM Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang: The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells, J Neurosci 25, 10546-55, 2005
Ovine Ovine, adult and fetal Adrenocortical Collagenase Type 1: 0.4%
 DMEM/Ham's F12 Valego, N. and Rose, J.: A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells., Reprod Sci 17, 477, 2010
Ovine Ovine, fetal Adrenocortical Collagenase Type 1: 0.4%
 DMEM/Ham's F12 Valego, N., Su, Y., Carey, L., Young, S., Tatter, S., Wang, J. and Rose, J.: Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression., Am J Physiol Regul Integr Comp Physiol 289, R410, 2005
Ovine Ovine, adult Chromaffin cells Collagenase Type 2: 0.2%
Deoxyribonuclease I: 100 u/ml
 Locke's solution Keating, D., Rychkov, G., Adams, M., Holgert, H., McMillen, I.C. and Roberts, M.: Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels., J Physiol 555, 489, 2004
Ovine Ovine, 3 year Anterior pituitary Trypsin: 2.5%
Deoxyribonuclease I: 0.004%
 DMEM Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J: The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep, J Endocrinol 162, 215-25, 1999
Rat Rat Chromaffin Collagenase Type 1: 0.1-0.2%
Deoxyribonuclease I: 0.003-0.015%
Hyaluronidase: 0.015%
 DMEM/F12 Dominguez, N., Rodri­guez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012
Rat Rat Chromaffin Collagenase Type 1: 0.26%
Deoxyribonuclease I: 0.015%
Hyaluronidase: 0.015%
 HBSS Gilabert, J, Montalvo, G, and Artalejo A.: Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays, Nat Protoc , 294, 2006
Rat Rat, SD Chromaffin cells Collagenase Type 1: 0.26%
Deoxyribonuclease I: 0.015%
Hyaluronidase: 0.015%
 HBSS Gilabert, J: Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture,Cell Biology of the Chromaffin Cell,Borges, R. and Gandia, L., Instituto Teofilo Hernando, , 2004
Rat Rat, SD, male Zona fasciculata/reticularis Collagenase: 0.4%
 Krebs-HEPES Bruder Eric D, Ball Dennis L, Goodfriend Theodore L, Raff Hershel: An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells, Am J Physiol Regul Integr Comp Physiol 284, R1631-5, 2003
Rat Rat, Wistar, newborn Chromaffin cells Collagenase Type 1: 0.025%
Deoxyribonuclease I: 0.0015%
 DMEM Zhang L, Castell A, Avila E, Drucker-ColĂ­n R, Escobar A: Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons, J Neuropathol Exp Neurol 59, 170-4, 2000
Rat Rat, SD, male ZG
ZFR		 Collagenase Type 1: 0.2%
 Kreb's Sayed, S., Whitehouse, B., and Jones, P.: Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis, J Endocrinol 154, 449, 1997
Rat Rat, Fischer, male, 10-16 weeks Adrenocortical Deoxyribonuclease I: 0.005%
 BSS Roskelley, C.D. and Auersperg, N.: Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture, In Vitro Cell Dev Biol 26, 493, 1990
Rat Rat, male, 120-160 g,
Rat, SD, male, 400-450 g		 Leydig
Adrenal		 Collagenase Type 2: 0.03% (adrenal)
 Krebs Ringer bicarbonate buffer Ng, T. and Liu, W.: Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis, In Vitro Cell Dev Biol 26, 24, 1990
Rat Rat, SD, 2-4 day old
(also bovine)		 Heart
Adrenal chromaffin
Paraneurons		 Trypsin: 0.06%
 25mM HEPES buffered Locke's solution, CMF Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
Rat Rat, Long-Evans, female, 150-200 g Glomerulosa Collagenase: 0.2%
 MEM-d-Val Payet, N., Deziel, Y., and Lehoux, J.-G.: Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture, J Steroid Biochem 20, 449, 1984
Rat Rat, Fischer, male, 1-10 months Adrenocortical Deoxyribonuclease I: 0.005%
 BSS Leonard, R.K., Auersperg, N., and Parkes, C.O.: Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells, In Vitro 19, 46, 1983
Rat Rat, SD, male, 400-450 g Decapular
Capsular
Glomerulosa		 Deoxyribonuclease I: 0.01%
 Medium 199 Li, C.H., Ng, T.B., and Cheng, C.H.K.: Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells, Int J Pept Protein Res 19, 361, 1982
Rat Rat Chromaffin Trypsin: 0.10%
 Ham's F-12 w/HEPES Englert, D.F.: An Optical Study of Isolated Rat Adrenal Chromaffin Cells, Exp Cell Res 125, 369, 1980
Rat Rat, Hanover-Wistar, 2nd postnatal week (also guinea-pig, cattle) Chromaffin Collagenase: 0.5%
Collagenase: 0.5%
 HBSS Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255, 51, 1980
Rat Rat , SD, female, 200 g Glomerulosa Deoxyribonuclease I: 0.05%
 Kreb's Braley, L., Williams, G., and Bradwin, G.: The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells, Endocrinology 106 (1), 50, 1980
Rat Rat Foreskin Collagenase: 0.5%
 Dulbecco's MEM w/10% calf serum Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979
Rat Rat, Wistar-Hanover, 7-12 day Medullary Trypsin: 0.125%
 HBSS Unsicker, K., Krisch, B., Otten, U., and Thoenen, H.: Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids, Proc Natl Acad Sci U S A 75 (7), 3498, 1978
Rat Rat, SD, male Cortical Trypsin: 0.25%
 Kreb's Ringer bicarbonate buffer Barofsky, A., Feinstein, M., and Halkerston, I.: Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands, Exp Cell Res 79, 263, 1973
Rat Rat, Holtzman, male, 180-250 g Adrenal Collagenase Type 1: 0.5%
 Kreb's Ringer bicarbonate buffer Kloppenborg, P., Island, D., Liddle, G., Michelakis, A., and Nicholson, W.: A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism, Endocrinology 82, 1053, 1968