Adipose/Fat - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Gerbil Gerbil of unknown age
(also rat, hamster, rabbit, lamb, guinea-pig)		 Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer The Brown Fat Cell , Int Rev Cytol 74, 187, 1982
Guinea-Pig Guinea-pig, adult
(also rat, hamster, gerbil, rabbit, lamb)		 Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer The Brown Fat Cell , Int Rev Cytol 74, 187, 1982
Hamster Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig)		 Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer The Brown Fat Cell , Int Rev Cytol 74, 187, 1982
Human human adipocytes Collagenase Type 1: 0.2%
 HBSS Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat. , J Endocrinol 192, 279-88, 2007
Human Human, adult, obese Adipocytes Collagenase Type 2: 0.1%
 DMEM/F12 Somatostatin Is Expressed and Secreted by Human Adipose Tissue Upon Infection and Inflammation , J Clin Endocrinol Metab 89(10), 4833, 2004
Human Human, adult, female Preadipocytes Collagenase Type 1: 0.2%
 DMEM/F-12 Androgen generation in adipose tissue in women with simple obesity--a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5 , J Endocrinol 183, 331, 2004
Human human, adult adipocytes Collagenase Type 1: 0.13%
 see reference Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans. , Endocrinology 145, 2273, 2004
Human Human, adult, female, non-obese Adipocytes Collagenase Type 2: 0.05%
 DMEM Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression. , Horm Metab Res 35, 158, 2003
Human Human Adipocytes Collagenase Type 1: see reference
 Saline Discernment of Adipose versus Nervous Tissue: A Novel Adjunct Solution in Lipomyelomeningocele Surgery. , Ped Neurosurg 36, 314, 2002
Human Human, Adult, male and female Adipocytes Collagenase Type 1: 0.2%
 DMEM:F12 Glucocorticoid Regulation of P450 Aromatase Activity in Human Adipose Tissue: Gender and Site Differences , J Clin Endocrinol Metab 87, 1327, 2002
Human Adult, male and female Adipocytes Collagenase Type 1: 0.1%
 DMEM/Ham's F-12 Depot-specific Release of Leptin from Subcutaneous and Omental Adipocytes in Suspension Culture: Effect of Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta1. , Eur J Endocrinol 141 (4), 436, 1999
Human Human, adult, male and female Adipocytes Collagenase Type 1: 0.1%
 DMEM: F12 Intrinsic Site-Specific Differences in the Expression of Leptin in Human Adipocytes and Its Autocrine Effects on Glucose Uptake , J Clin Endocrinol Metab 84, 2550, 1999
Human Human, non-diabetic, male Adipocytes Collagenase Type 1: 0.05%
 Kreb's Ringer bicarbonate buffer Receptor Binding and Biological Effect of Insulin in Human Adipocytes , Diabetologia 13, 589, 1977
Mouse mouse, C57Bl/6J adipocytes Collagenase Type 1: 0.15%
 DMEM/F12 Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes, J Health Sci 51, 21, 2005
Mouse mouse, 3-6 day and 2-3 month adipose-derived stromal cells Collagenase Type 2: 0.075%
 PBS Adipose-derived adult stromal cells heal critical-size mouse calvarial defects. , Nat Biotechnol 22, 560-7, 2004
Mouse Mouse, C57BL/6J and FVB, male, 8-9 week Adipocytes Collagenase:
DMEM Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes , J Biol Chem 278, 47585, 2003
Mouse Mouse, both sexes Adipocytes Collagenase: 0.05%
 Krebs-Ringer Phosphate HEPES (KRPH) Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice. , Am J Physiol/Endo 281, E1249, 2001
Mouse Mouse, B6D2F1, F1 hybrids, New Zealand black female & New Zealand white male Vascular endothelial Collagenase: 0.2%
 PBS The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue , J Immunol Methods 102, 45, 1987
Porcine Pig, crossbred, male 1-4 day Adipocytes Collagenase Type 1: 0.2%
 DMEM/F12 Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes , J Anim Sci 81, 3008, 2003
Porcine Pig, 8-9 week Adipose Collagenase Type 1: 300 u/ml
 HEPES Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo , Comp Biochem Physiol B 123, 307, 1999
Porcine Crossbred, 1-3 day Adipose, Stromal-vascular Collagenase Type 1: 0.2%
 DMEM/F12 Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture , J Anim Sci 75, 105, 1997
Rat Rat, Winstar, 4 week Adipocytes Collagenase: 0.2%
 Ham's F12 Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro , Cell Struct Funct 28, 55, 2003
Rat Rat, SD Adipocytes Collagenase Type 1: 0.2%
 KRHB The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency , Endocrinology 142, 1999, 2001
Rat SD, male, 4-7 wks Brown adipocytes Deoxyribonuclease I: 0.5%
 DMEM Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes. , J Physiol 521 (3), 601, 1999
Rat Rat Adipocytes Collagenase Type 2: 0.2%
 DMEM /F-12 , Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 11B:6.1, 1995
Rat Rat, SD, male, 130-160 g Adipose Epididymal fat pads Collagenase: 0.3%
 Kreb's-Ringer bicarbonate buffer modified Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose Cells In Vivo , J Biol Chem 265, 7994, 1990
Rat Rat, 3 day Preadipocytes Collagenase Type 3: 0.10%
 Parker Medium 199 Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture , Biochim Biophys Acta 968, 231, 1988
Rat Rat, SD, various weights and ages Brown adipocytes Interscapular & cervical depots Collagenase: 0.2%
Soybean Trypsin Inhibitor: 0.3%
 Krebs Ringer bicarbonate buffer Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline , Biochem J 238, 395, 1986
Rat Rat, CD, male, 150-200 g Adipocytes Epididymal fat pads Collagenase: 0.1%
 Krebs Ringer bicarbonate buffer B-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes , J Biol Chem 258, 7386, 1983
Rat Rat, Wistar, albino, male, 100-140 g Adipocytes Epididymal-fat pads Collagenase: 0.3%
 Kreb's Ringer Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites , Biochem J 180, 365, 1979
Rat Rat, Fischer, 344, male, 9 - 13 week White fat Collagenase: 0.3%
 Kreb's Ringer bicarbonate buffer Influence of Age on Size an Number of Fat Cells in the Epididymal Depot , Am J Physiol 229, 1561, 1975
Rat Rat (CFE), albino, female Brown fat Collagenase Type 1: 0.10%
 Bicarbonate buffer Isolation and Metabolism of Brown Fat Cells , J Biol Chem 242, 1887, 1967
Rat Rat, SD, male, 160-210 g Fat Collagenase: 0.3%
 Albumin-bicarbonate buffer Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis , J Biol Chem 239 (2), 375, 1964