Adipose/Fat - Tissue Tables
| Species | Species Detail | Cells | Enzyme(s) | Medium |
Reference |
| Gerbil
|
Gerbil of unknown age
(also rat, hamster, rabbit, lamb, guinea-pig) |
Brown fat |
Collagenase Type 1: 0.10%
|
Bicarbonate buffer |
The Brown Fat Cell
,
Int Rev Cytol 74,
187, 1982 |
| Guinea-Pig
|
Guinea-pig, adult
(also rat, hamster, gerbil, rabbit, lamb) |
Brown fat |
Collagenase Type 1: 0.10%
|
Bicarbonate buffer |
The Brown Fat Cell
,
Int Rev Cytol 74,
187, 1982 |
| Hamster
|
Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig) |
Brown fat |
Collagenase Type 1: 0.10%
|
Bicarbonate buffer |
The Brown Fat Cell
,
Int Rev Cytol 74,
187, 1982 |
| Human
|
human |
adipocytes |
Collagenase Type 1: 0.2%
|
HBSS |
Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat.
,
J Endocrinol 192,
279-88, 2007 |
| Human
|
Human, adult, obese |
Adipocytes |
Collagenase Type 2: 0.1%
|
DMEM/F12 |
Somatostatin Is Expressed and Secreted by Human Adipose Tissue Upon Infection and Inflammation
,
J Clin Endocrinol Metab 89(10),
4833, 2004 |
| Human
|
Human, adult, female |
Preadipocytes |
Collagenase Type 1: 0.2%
|
DMEM/F-12 |
Androgen generation in adipose tissue in women with simple obesity--a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5
,
J Endocrinol 183,
331, 2004 |
| Human
|
human, adult |
adipocytes |
Collagenase Type 1: 0.13%
|
see reference |
Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans.
,
Endocrinology 145,
2273, 2004 |
| Human
|
Human, adult, female, non-obese |
Adipocytes |
Collagenase Type 2: 0.05%
|
DMEM |
Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression.
,
Horm Metab Res
35,
158, 2003 |
| Human
|
Human |
Adipocytes |
Collagenase Type 1: see reference
|
Saline |
Discernment of Adipose versus Nervous Tissue: A Novel Adjunct Solution in Lipomyelomeningocele Surgery.
,
Ped Neurosurg 36,
314, 2002 |
| Human
|
Human, Adult, male and female |
Adipocytes |
Collagenase Type 1: 0.2%
|
DMEM:F12 |
Glucocorticoid Regulation of P450 Aromatase Activity in Human Adipose Tissue: Gender and Site Differences
,
J Clin Endocrinol Metab 87,
1327, 2002 |
| Human
|
Adult, male and female |
Adipocytes |
Collagenase Type 1: 0.1%
|
DMEM/Ham's F-12 |
Depot-specific Release of Leptin from Subcutaneous and Omental Adipocytes in Suspension Culture: Effect of Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta1.
,
Eur J Endocrinol 141 (4),
436, 1999 |
| Human
|
Human, adult, male and female |
Adipocytes |
Collagenase Type 1: 0.1%
|
DMEM: F12 |
Intrinsic Site-Specific Differences in the Expression of Leptin in Human Adipocytes and Its Autocrine Effects on Glucose Uptake
,
J Clin Endocrinol Metab 84,
2550, 1999 |
| Human
|
Human, non-diabetic, male |
Adipocytes |
Collagenase Type 1: 0.05%
|
Kreb's Ringer bicarbonate buffer |
Receptor Binding and Biological Effect of Insulin in Human Adipocytes
,
Diabetologia 13,
589, 1977 |
| Mouse
|
mouse, C57Bl/6J |
adipocytes |
Collagenase Type 1: 0.15%
|
DMEM/F12 |
Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes,
J Health Sci 51,
21, 2005 |
| Mouse
|
mouse, 3-6 day and 2-3 month |
adipose-derived stromal cells |
Collagenase Type 2: 0.075%
|
PBS |
Adipose-derived adult stromal cells heal critical-size mouse calvarial defects.
,
Nat Biotechnol
22,
560-7, 2004 |
| Mouse
|
Mouse, C57BL/6J and FVB, male, 8-9 week |
Adipocytes |
Collagenase:
|
DMEM |
Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes
,
J Biol Chem
278,
47585, 2003 |
| Mouse
|
Mouse, both sexes |
Adipocytes |
Collagenase: 0.05%
|
Krebs-Ringer Phosphate HEPES (KRPH) |
Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice.
,
Am J Physiol/Endo 281,
E1249, 2001 |
| Mouse
|
Mouse, B6D2F1, F1 hybrids, New Zealand black female & New Zealand white male |
Vascular endothelial |
Collagenase: 0.2%
|
PBS |
The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue
,
J Immunol Methods 102,
45, 1987 |
| Porcine
|
Pig, crossbred, male 1-4 day |
Adipocytes |
Collagenase Type 1: 0.2%
|
DMEM/F12 |
Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes
,
J Anim Sci 81,
3008, 2003 |
| Porcine
|
Pig, 8-9 week |
Adipose |
Collagenase Type 1: 300 u/ml
|
HEPES |
Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo
,
Comp Biochem Physiol B
123,
307, 1999 |
| Porcine
|
Crossbred, 1-3 day |
Adipose, Stromal-vascular |
Collagenase Type 1: 0.2%
|
DMEM/F12 |
Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture
,
J Anim Sci 75,
105, 1997 |
| Rat
|
Rat, Winstar, 4 week |
Adipocytes |
Collagenase: 0.2%
|
Ham's F12 |
Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro
,
Cell Struct Funct 28,
55, 2003 |
| Rat
|
Rat, SD |
Adipocytes |
Collagenase Type 1: 0.2%
|
KRHB |
The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency
,
Endocrinology 142,
1999, 2001 |
| Rat
|
SD, male, 4-7 wks |
Brown adipocytes |
Deoxyribonuclease I: 0.5%
|
DMEM |
Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes.
,
J Physiol
521 (3),
601, 1999 |
| Rat
|
Rat |
Adipocytes |
Collagenase Type 2: 0.2%
|
DMEM /F-12 |
,
Cell & Tissue Culture: Laboratory Procedures,Doyle, A., Griffiths, J., and Newell, D.,
John Wiley and Sons, Ltd.,
11B:6.1, 1995 |
| Rat
|
Rat, SD, male, 130-160 g |
Adipose
Epididymal fat pads |
Collagenase: 0.3%
|
Kreb's-Ringer bicarbonate buffer modified |
Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose Cells In Vivo
,
J Biol Chem
265,
7994, 1990 |
| Rat
|
Rat, 3 day |
Preadipocytes |
Collagenase Type 3: 0.10%
|
Parker Medium 199 |
Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture
,
Biochim Biophys Acta
968,
231, 1988 |
| Rat
|
Rat, SD, various weights and ages |
Brown adipocytes
Interscapular & cervical depots |
Collagenase: 0.2%
Soybean Trypsin Inhibitor: 0.3%
|
Krebs Ringer bicarbonate buffer |
Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline
,
Biochem J
238,
395, 1986 |
| Rat
|
Rat, CD, male, 150-200 g |
Adipocytes
Epididymal fat pads |
Collagenase: 0.1%
|
Krebs Ringer bicarbonate buffer |
B-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes
,
J Biol Chem
258,
7386, 1983 |
| Rat
|
Rat, Wistar, albino, male, 100-140 g |
Adipocytes
Epididymal-fat pads |
Collagenase: 0.3%
|
Kreb's Ringer |
Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites
,
Biochem J
180,
365, 1979 |
| Rat
|
Rat, Fischer, 344, male, 9 - 13 week |
White fat |
Collagenase: 0.3%
|
Kreb's Ringer bicarbonate buffer |
Influence of Age on Size an Number of Fat Cells in the Epididymal Depot
,
Am J Physiol 229,
1561, 1975 |
| Rat
|
Rat (CFE), albino, female |
Brown fat |
Collagenase Type 1: 0.10%
|
Bicarbonate buffer |
Isolation and Metabolism of Brown Fat Cells
,
J Biol Chem
242,
1887, 1967 |
| Rat
|
Rat, SD, male, 160-210 g |
Fat |
Collagenase: 0.3%
|
Albumin-bicarbonate buffer |
Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis
,
J Biol Chem
239 (2),
375, 1964 |
|
|